“遇邪”与清代假命案的形成与处理——以《巴县档案》为中心的讨论

有清一代,凡最终确定并不构成关系人犯罪的命案称为假命案。在对假命案的处理过程中,涉案人及州县官员均有借用“遇邪”之类理据来促成案件和解的行为。虽然百姓以死者“遇邪”为由和解命案的背后多隐含金钱因素的推动,但知县为了顺利结案,对于这一结果往往乐见其成,甚至在审案过程中还存在一些主动引导涉案人确认死者遇邪身亡之举。然而,由于朝廷以儒家思想规范社会,总体上不容妖邪鬼怪之说,州县在上呈详文中又往往隐去“遇邪”的死因记录,以致档案记载的司法状况多呈混乱。在当时社会环境下,此类司法实践存在一定的合理性,但同时也必须看到,这种司法实践会造成案情中许多对社会生活史和法制史研究有重要意义的真实细节的遗失。

Long TR three-dimensional inversion recovery sequence with real reconstruction(3D real IR)for quantifying inner ear endolymphatic hydrops

Objective To observe the value of long TR three-dimensional inversion recovery sequence with real reconstruction(3D real IR)for quantifying inner ear endolymphatic hydrops(EH).Methods Totally 46 Ménière's disease(MD)patients and 21 healthy volunteers were prospectively enrolled.MR scanning for inner ear based on 3D real IR and 3D fluid attenuated inversion recovery(3D FLAIR)sequence 4—6 h after administration of contrast agents were performed.The imaging qualities were scored and compared between groups.The endolymphatic space area and the membranous labyrinth area of cochlea and vestibule,as well as endolymph/membranous labyrinth area percentage were calculated,the present or not of EH and the grade of EH were evaluated.EH inner ears of MD patients were enrolled in EH group,while inner ears of healthy volunteers were taken as controls(control group).The endolymphatic space area,membranous labyrinth area and endolymph/membranous labyrinth area percentage of cochlea and vestibule were compared between groups.The receiver operating characteristic(ROC)curve was drawn to calculate the diagnostic efficacy of the above indexes.Results Cochlear and/or vestibular EH were detected in 56 ears,including cochlear EH in 52 ears and vestibular EH in 45 ears among 46 MD patients(EH group),but not in 42 ears in control group.The subjective quality scores of 3D real IR images were higher than those of 3D-FLAIR(both P<0.05).Quantitative analysis based on 3D real IR images revealed that,compared with control group,significantly larger endolymph areas and endolymph/membranous labyrinth area percentages in both cochlea and vestibule were found in EH group(all P<0.001).The area under the curve(AUC)of cochlear or vestibular endolymph/membranous labyrinth area percentage for identifying inner ear EH was 0.999 and 0.985,respectively.Taken 13.64%and 24.13%as the critical value of cochlear or vestibular endolymph,the specificity was 100%and 92.86%,respectively,and the sensitivity was 96.43%and 96.43%,respectively.Conclusion MR long TR 3D real IR was helpful to quantifying inner ear EH.

SOX2/DRD2 signaling pathway facilitates astrocytic dedifferentiation in cerebral ischemic mice

Objective:To explore the effects of dopamine receptor D2(DRD2)on astrocytic dedifferentiation based on SOX2-regulated genes in neural stem cells(NSCs)and astrocytes.Methods:Immunofluorescence staining and SOX2-GFP mice were used to examine the lineage differentiation of SOX2-positive cells during the development of cerebral cortex.Primary NSCs/astrocytes culture,ChIP-seq and Western Blot were adopted to analyze and verify the expression of candidate genes.Pharmacological manipulation,neurosphere formation,photochemical ischemia,immunofluorescence staining and behavior tests were adopted to evaluate the effects of activating DRD2 signaling on astrocytic dedifferentiation.Results:Immunofluorescence staining demonstrated the NSC-astrocyte switch of SOX2-expression in the normal development of cerebral cortex.ChIP-seq revealed enrichment of DRD2 signaling by SOX2-bound enhancers in NSCs and SOX2-bound promoters in astrocytes.Western Blot and immunofluorescence staining verified the expression of DRD2 in NSCs and reactive astrocytes.Application of quinagolide hydrocholoride(QH),an agonist of DRD2,significantly promoted astrocytic dedifferentiation both in vitro and in vivo following ischemia.In addition,quinagolide hydrocholoride treatment improved locomotion recovery.Conclusion:Activating DRD2 signaling facilitates astrocytic dedifferentiation and may be used to treat ischemic stroke.

BANACH ALGEBRA STRUCTURE IN DERIVATIVE HARDY SPACES

In this paper,we consider the algebraic structure of derivative Hardy Spaces.By using the method of[6,12,15],we get the Duhamel product forming Banach algebra in derivative Hardy Spaces,and invertibility criterion,and describe the extended eigenvalue of the integral operator V.We generalize the results in[1,2,6,11,16].

AFLP Fingerprinting Analysis of Elite Hevea brasiliensis Germplasm

There are more than 6 000 clones of Hema brasiliensis Mull. Ary germplasm in the germplasm garden of Chinese National Key Biotechnology Laboratory for Tropical Crops and some of them are elite germplasm demonstrated by production and previous studies. AFLP (amplified fragment length polymorphism) fingerprinting analysis was performed on 25 clones (15 of Wickham clones and 10 of Amazon wild clones which possess phenotypes with high-yielding,/Iow-yielding, cold-tolerance/cold-sensitivity, oidium-resistance/oidium-sensitivity, tapping panel dryness (TPD) /healthy) respectively through a 377 DNA sequencer (P. E. Corp.) and PAGE electrophoresis results were analyzed by using GeneScan(TM) and Genotype(TM) Analysis software (P. E. Corp. The fragment profiles of different clones were obtained. Five hundred and eighteen fragments were generated by two primer combinations screened from 64 primer combinations and 511 fragments appeared to be polymorphic (98.6%). Genetic distance ranged from 0.25 to 0.81 between clones and ranged from 0.07 to 0.17 within RRIM600 clone. A specific 320 bp fragment of the oidium-resistant clones was found through genotype analysis. These results showed that AFLP fingerprints were highly reproducible and powerful and can be widely used in germplasm identification and genetic diversity analysis of Hema brasiliensis. In addition, based on the AFLP data, cluster analysis was performed. Cluster results showed that all the clones studied were almost clustered into a group one by one.

Chemical constituents from Pithecellobium clypearia and their effects on T lymphocytes proliferation

Aim To investigate the chemical constituents from the twigs and leaves of Pithecellobium clypearia Benth and their immunomodulatory effects. Methods The constituents were separated and purified by various chromatographic methods and their structures were identified on the basis of spectral analysis. The immufiomodulatory effects of all the compounds were examined by a Con A-induced T lymphocytes proliferation assay. Results Eight compounds were isolated and identified as （-）- epigallocatechin （1）, （-）-5, 7, 3′, 4′, 5′-pentahydroxyflavan （2）, （-）-epigallocatechin-7-gallate （3）, （-）-5, 3′, 4′, 5′-tetrahydroxyfiavan- 7-gallate （4）, quercitin-3-O-α-L-rhamnpyranoside （5）, myricitin-3-O-α-L-rhamnpyranoside （6）, gallic acid （7）, and ethyl gallate （8）, respectively. Conclusion Compounds 3 and 8 were isolated from this genus for the first time, and compound 1 was isolated from this species for the first time. Compound 3 exhibited a strong inhibition on the T lymphocytes proliferation induced by Con A with an IC50 of 4.4 μmol·L^-1.

Recent advances in immobilized enzymes on nanocarriers

Recent progress in nanotechnology has provided high-performance nanomaterials for enzyme immobilization.Nanobiocatalysts combining enzymes and nanocarriers are drawing increasing attention because of their high catalytic performance,enhanced stabilities,improved enzyme-substrate affinities,and reusabilities.Many studies have been performed to investigate the efficient use of cellulose nanocrystals,polydopamine-based nanomaterials,and synthetic polymer nanogels for enzyme immobilization.Various nanobiocatalysts are highlighted in this review,with the emphasis on the design,preparation,properties,and potential applications of nanoscale enzyme carriers and nanobiocatalysts.

Localized Effects of Mechanical Wounding and Exogenous Jasmonic Acid on the Induction of Secondary Laticifer Differentiation in Relation to the Distribution of Jasmonic Acid in Hevea brasiliensis

The relationship between the effect of exogenous jasmonic acid (JA) on the induction of secondary laticifer differentiation and the distribution of JA in the seedling of Hevea brasiliensis Mull. Arg. was investigated with the aid of experimental morphological and radioisotope technique. Most radioactivity of H-3-JA sustained in treated site within one hour while no radioactivity was detected in new shoot and the radioactivity in upper leaf was much less than that in the parts below the treated site, suggesting that JA was mainly transported downwards in the shoot of H brasiliensis. Mechanical wounding hindered the entrance of exogenous JA remarkably while held back the entered JA to the regions around wounded site. The effect of exogenous JA and mechanical wounding on the induction of the secondary laticifer differentiation was limited to treated site where high level of JA was expected. Mechanical wounding reduced the effect of exogenous JA on the differentiation of secondary laticifer, which could be ascribed to the hindrance of mechanical wounding to the entrance of exogenous JA. It was concluded from the combined data that a high accumulation of JA was required for inducing the secondary laticifer differentiation in H. brasiliensis.

Mitochondria dysfunction was involved in copper-induced toxicity in MES23.5 cells

Objective To investigate the toxicity of copper on MES23.5 dopaminergic cells and the probable mechanisms involved in this process. Methods MES23.5 dopaminergic cells were selected as our experimental model. [3-（4, 5-dimethylthiazol2-yl）-2, 5 diphenyltetrazolium bromide] （MTT） assay was used to detect the influence of copper on the cell viability. The semiquantitative reverse transcription polymerase chain reaction （RT-PCR）, Western blotting and the high performance liquid chromatography-electrochemical detection （HPLC-ECD） have been used to detect the tyrosine hydroxlase （TH） mRNA and protein expression and the dopamine content in MES23.5 cells. The flow cytometry have been used to detect the changes of mitochondrial transmembrane potential. Results 100 and 200 μmol/L copper had no effect on the MES23.5 cell viability, whereas 400 and 800 μmol/L of copper could decrease the cell viability （P 〈 0.01）. Treating cells with 200 μmol/L copper for 24 h decreased the TH mRNA expression, the TH expression and the dopamine content compared with the control （P 〈 0.01, P 〈 0.01, P 〈 0.05, respectively）. Besides, the mitochondrial transmembrane potential also decreased with the treatment of 200 μmol/L copper for 24 h （P 〈 0.01）. Condusion Copper could exert the toxic effects on MES23.5 dopaminergic cells and decrease the cell function. The dysfunction of mitochondria may be the mechanism of this toxicity effect.

Experimental Study of Chinese Ink as a New Type of Dye Tracer in Sentinel Lymph Node Biopsy~*

Objective： To investigate the potential clinical value of Chinese ink as a tracer in dye-directed sentinel lymph node biopsy. Methods： Forty-two female rabbits were randomly divided into 7 groups. The breast drainage lymphatic vessels and nodes in axilla were exposed by surgery. Under the papillae of the second pair of breast, 0.1 mL Chinese ink dilution at concentrations of 0.1%, 1%, 10%, 50% and 100% and isosulfan blue （IB, 1.0%） were injected. The movement of ink in lymphatic chain was investigated, and the number of stained nodes and their staining and washout time ware recorded. The first stained lymph node that was followed through lymphatic drainage was identified as the sentinel lymph node （SLN）. The SLNs were dissected and prepared for histological observation 5 rain, 1 h and 2 weeks after stained. Blood samples were harvested and tested before and 2 weeks after injection. The hearts, lungs, livers and kidneys were dissected after a period of post-operation observation and sent for pathologic examination. Results： No obvious differences were observed among the staining time of SLNs and the second lymph node using ink at different concentrations （P〉0.05）. But they were obviously longer than that of IB （P〈0.05）. The mean number of nodes obtained was nearly the same （P〉0.05）. It was too light for gross visual inspection when nodes were stained by ink at the concentration of 0.1%. Nodes stained by IB faded 33.9 rain after stained. And carbon staining seemed permanent without any visible decline after 2 weeks. Carbon particles were found to be in the sinus of lymph nodes that were obtained 5 rain after stained, and then was phagocytosed by macrophages （MФ） 1 h later. The density of carbon obscured the microscopic observation of lymph nodes when they were stained using Chinese ink at concentration of higher than 20.0%. No obvious changes were investigated in blood test and vital organ pathologic examination. Conclusion： Chinese ink is potentially a novel type of dye tracer in SLN biopsy.

A Systematic Revision of "Barbodes" Fishes in China

The genus Barbodes Bleeker is a catchall group.The species assigned to this genus in China actually belong to at least four distinct genera.After comparing all the Barbodes fishes included in this research to the system used by Rainboth,reassign Barbodes fishes in China as following:①B.baoshanensis,B.benasi and B.heterostomus are assigned to genus Neolissochilus;②B.parvus are assigned as Discherodontus parvus;③B.pierrei in Yunnan is conspecific with B.vernayi and B.vernayi should be assigned to the genus Hypsibarbus as Hypsibarbus vernayi;④the remaining Barbodes fishes,B.fuxianhuensis,B.chonglingchungi,B.margarianus,B.huangchuchieni,B.cogginii (=B.daliensis),B.rhomboides,B.carinatus,B.opisthopterus and B.exiguus are assigned to genus Poropuntius.

Static and dynamic tensile failure characteristics of rock based on splitting test of circular ring

Static and dynamic splitting tests were conducted on ring marble specimens with different internal diameters to study the tensile strength and failure modes with the change of the ratio of internal radius to external radius （ρ） under different loading rates. The results show that the dynamic tensile strength of disc rock specimen is approximately five times its static tensile strength. The failure modes of ring specimens are related to the dimension of the internal hole and loading rate. Under static loading tests, when the ratio of internal radius to external radius of the rock ring is small enough （ρ〈0.3）, specimens mostly split along the diametral loading line. With the increase of the ratio, the secondary cracks are formed in the direction perpendicular to the loading line. Under dynamic loading tests, specimens usually break up into four pieces. When the ratio ρreaches 0.5, the secondary cracks are formed near the input bar. The tensile strength calculated by Hobbs’ formula is greater than the Brazilian splitting strength. The peak load and the radius ratio show a negative exponential relationship under static test. Using ring specimen to determine tensile strength of rock material is more like a test indicator rather than the material properties.

Evaluation on Uncertainty of Determining Aspartame in Beverage by HPLC

[Objective] The aim was to evaluate the uncertainty of determining aspartame in beverage by high performance liquid chromatography （ HPLC）. [Method] The content of aspartame in beverage was determined by HPLC, then the source of uncertainty in the whole determination process was analyzed, and each component of uncertainty was evaluated and combined. [ Result] Through 6 repeated determinations by the method in GB/T 22254-2008 ＂Determination of Aspartame in Food＂, the average content of aspartame in beverage was （0.806 ±0.038） g/kg, and k =2. The main sources of uncertainty to affect the process were the sample weighting process, the preparation process of standard solution introduced by sample constant volume and the uncertainty introduced by fitting standard curve. ①The uncertainty of standard work-solution. The combined uncertainty of standard work-solution was 0.013 9, among them the uncertainty introduced by standard sample purity was 0.005 8, the standard uncertainty introduced by standard material weighting was 1.49 ×10^4, the relative uncertainty introduced by glass apparatus calibration in the preparation process of aspartame standard reserving solution was 0. 007 88, and the uncertainty introduced by glass apparatus calibration in the preparation process of standard work-solution was 0. 009 9. ②The uncertainty introduced by the preparation process of sample specimen. Among them the relative standard uncertainty introduced by sample weighting process was 0. 009, and the uncertainty introduced by sample constant volume was 0.000 78. ③The uncertainty introduced by the fitting process of standard curve. Among them the relative uncertainty of curve fitting was 0.002 46, the uncertainty introduced by the determined results of aspartame was 0.017 0, the total combined standard uncertainty was 0.023 9, and the expanded standard uncertainty was 0.019. [ Conclusion] The uncertainty components of standard solution, standard curve and repeatability are the main sources of uncertainty, while those of sample weighting and sample constant volume account for little proportion.

ANALYSIS OF WORKING MODES OF HYBRID EXCITATION DOUBLY SALIENT MOTOR

A more accurate analysis method on working modes is proposed by considering the winding terminal voltage and the eondueting power device as state parameters. For the three-phase hybrid excitation doubly salient machine （HEDSM） motor and its three-phase full-bridge inverter, in the proposed analytical method, all possible working modes are generally listed. Then, with the H_PWM-L_ON control strategy, the working modes are detailed with eorresponding equivalent circuits. Experimental results verify the robustness of the analysis.

Analysis of Genetic Relationship among Medicago sativa,Medicago falcate and Trigonella foenum-graecum Using ISSR

[Objective] This study aimed to analyze the genetic relationships among Medicago sativa,Medicago falcata and Trigonella foenum-graecum.[Method] ISSR technique was adopted to determine their genetic relationships.[Result] M.sativa,M.falcate and T.foenum-graecum had a broad genetic base.T.foenum-graecum shared closer relationship with M.falcata rather than M.sativa.The study on relationship between M.sativa and T.foenum-graecum was advantageous to identify disputable transition types.But a boundary should be found to identify species to be M.sativa or T.foenum-graecum.[Conclusion] This study will provide reference for identifying some disputable transition types.

Diagnose of Rabbit Pasteurellosis and Colibacillosis Mixed Infection and Drug Sensitive Test

In order to diagnose diseased rabbits, dead rabbits were dissected, and 4 strains, DX01, DX02, JX01 and JX02 were obtained from bacterial isolation and culture. According to conventional biochemical method, physiological-biochemical test showed that DX01 and DX02 could produce acids and gases in the presence of mannitol, lactose, glucose and maltose, and did not ferment sucrose, reactions of sodium citrate, urea, V-P, hydrogen sulfide and gelatin were negative, while reactions of indole, M-R and nitrate were positive, which were in accordance with the characteristics of colibacillus; and JX01 and JX02 both fermented mannitol, sucrose, glucose, maltose, and did not ferment lactose, reactions of indole, M-R, V-P, hydrogen sulfide and gelatin were negative, while the reaction of nitrate was positive, which were in accordance with the characteristics of Pasteurella. 16S rDNA detection and sequencing showed that JX01 and JX02 shared 100% homology with Pas- teurella. The animal inoculation test of isolated bacteria showed that the mice in the experiment groups all died within 2 d. Original cultures were isolated from dead mice. DX01 and DX02 were identified as colibacillus, JX01 and JX02 were identified as Pasteurella, and the death of diseased rabbits was caused by mixed infection of pasteureUosis and colibacillosis. Drug sensitive test showed that DX01 and DX02 were only sensitive to kanamycin, ofloxacin and sulfamethoxydiazine, and not sensitive to other drugs, and JX01 and JX02 were sensitive to ofloxacin, sulfamethoxydiazine, gentamicin, furazolidone, doxycycline, streptomycin, trimoxazole and spectinomycin, and were moderately sensitive to cefradine. Ofloxacin and sulfamethoxydiazine are preferable for the clinical treatment of this disease.

Effects of Traditional Chinese Medicine on Numbers of Lymphocytes and Goblet Cells in Villus Epithelia of Layers under Heat Stress

[Objective] This study aimed to investigate the effects of traditional Chinese medicine additives on numbers of lymphocytes and goblet cells in villus epithelia of layers under heat stress.[Method] A total of 180 88-d-old healthy Esa Brown cocks were selected.They were randomly divided into 9 experimental groups：Room temperature control,High temperature control,VC addition group,High formula I,Moderate formula I,Low formula I,High formula II,Moderate formula II and Low formula II.The formula I and formula II were to add different herbal extracts to the diet of cocks with different doses.The cocks in the VC addition group were administered orally with same-concentration VC solution.After certain time,the cocks were slaughtered.Then the numbers of epithelial lymphocytes and goblet cells in various segments of small intestine were counted by using conventional histological section and HE staining.[Result] The numbers of lymphocytes and goblet cells in villus epithelia of layers under heat stress were decreased gradually with the proceeding of experiment.The herbal extracts of formula I and formula II all could promote the generation of lymphocytes and goblet cells.But the promoting effect of formula II was best.[Conclusion] The Chinese herbal medicine additives have a good relieving effect on heat stress in layers.

Molecular Identification of Gallibacterium from Hen and Analysis of Its 16S rRNA Gene

Two strains of Gallibacterium, isolated from one laying hen flock in Zhengzhou City of Henan Province, were identified by the morphological observation, genus -specific PCR, and analysis of 16S rRNA gene, which was used to generate the phylogenetic tree, with the 21 members of the 12 genera belonging to Pasteurellaceae to analyze the homology. Two strains were named Yu-ZZ-HL-I-SLG and Yu-ZZ-HL-II-GZ. The comparative result of the 16S rDNA sequence shows that the 2 isolated strains are identical in sequence; the highest identity （99.9%） was observed between the isolated strain and one of the strains of Gallibacterium anatis （AF22.8002）, the homologies between the isolated strain and 3 strains of gallibacterium accessed in NCBI （AF22.8016, Gallibacterium genomosp. 1, AF228017, Gallibacterium genomosp. 2, AF22.8018, Gal- libacterium genomosp. 1 ） were above 97.1%, higher than that of the isolated strain and the other strains of the other 11 genera which were between 90.7% -93.2%. It can be seen from the phylogenetic tree that the 2 isolated strains and the other 4 strain of gallibacterium fell into the same branch, furthermore the 2 isolated strains and the strain of Gallibacterium anatis locate in an internal branch, indicating that the 2 isolated strains belong to Gallibacterium anatis.

Effect of priming on the germination of Peltophorum dubium seeds under water stress

Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechani-cal dormancy. Seeds were primed in solutions of 0.2% Captan at 10 C and 27C, PEG 6000 -1.0 MPa at 10C and 27C, 0.5 mol KNO3, 0.75 Mol KNO3, 1.0 Mol KNO3. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27C. For the experiments of all the groups, osmotic potential were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2, and -1.4 MPa. P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to -1.0 M Pa. Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG -1.4 MPa.

《巴渝舞》名称考辨──土家族古代音乐史系列研究之二(1)

汉代以后的许多历史文献都说汉代有一种叫作"巴渝舞"的舞蹈。其实，汉代并无"巴渝舞"之名。所谓的《巴渝舞》，在汉人著作中都只作《巴俞》。"巴俞"的"俞"字不应读作"渝水"的"俞"，而应读作《楚辞·招魂》"吴蔡讴"的""，训为歌，《巴俞》即是巴歌的意思。巴人即今土家族的先民，《巴俞》是土家族先民的音乐。