On-Resistance Degradations Under Different Stress Conditions in High Voltage pLEDMOS Transistors and an Improved Method

The on-resistance degradations of the p-type lateral extended drain MOS transistor （pLEDMOS） with thick gate oxide under different hot carrier stress conditions are different, which has been experimentally investigated. This difference results from the interface trap generation and the hot electron injection, and trapping into the thick gate oxide and field oxide of the pLEDMOS transistor. An improved method to reduce the on-resistance degradations is also presented, which uses the field oxide as the gate oxide instead of the thick gate oxide. The effects are analyzed with a MEDICI simulator.

Inducible Expression of Translation Elongation Factor 1A Gene in Rice Seedlings in Response to Environmental Stresses

Differences of gene expression between salinity_stressed and control rice ( Oryza sativa L. ssp. indica ) cultivar “Zhaiyeqing 8' were compared using differential display PCR (DD_PCR) technique. Sequence analysis of one salt_inducible cDNA clone revealed that this clone represented a new member of rice translation elongation factor 1A (eEF1A) gene family and was tentatively named REF1A. Northern blot hybridization using REF1A fragment as a probe was performed to investigate the expression of rice translation elongation factor 1A gene in response to various environmental factors. It was observed that expression of the eEF1A gene in rice shoots was dramatically induced by salinity stress or exogenous application of abscisic acid (ABA). The induction of this gene by ABA stress occurred more quickly than that by salinity stress. In addition, expression of rice translation elongation factor 1A gene was also induced by drought (15% PEG6000), cold (4 ℃) or heat_shock (37 ℃) stresses. The results suggested that the induction of translation elongation factor 1A gene expression by environmental stresses might reflect the general adaptive response of rice plants to the adverse circumstances.

Site-directed Mutagenesis Based on Overlap Extension PCR

[Objective] To establish an efficient, convenient and economical method for site-directed mutagenesis. [Method] The target mutation was introduced into primers designed by DNAMAN5.0 software. Through overlap extension PCR for twice obtained the mutation gene which of the full length of the recombinant Human Tissue type plasminogen activator （Reteplase）. The mutation gene cloned it into pEASY- blunt simple cloning vector for sequencing. [Result] The sequencing results showed that three site mutations were fully consistent with the expected results （10~ site had been added a base-pair of A, C had been changed into G at 137~ site, G had been changed into A at 686~ site）.Three site mutations were introduced by using overlap extension PCR on one-step. The overall rate of obtaining the mutant sites was 100%. Site-directed mutagenesis will clone the recombinant Human Tissue type plas- minogen activator and laid the basis for the functional study. [Conclusion] Site-directed mutagenesis was successfully implemented based on the overlap extension PCR which is an efficient, convenient and economical DNA-directed mutagenesis method.

Site-directed Mutagenesis of Streptomyces avermitilis aveD Gene

[Objective] The aim of this study was to produce Streptomyces avermitilis strain with site-directed mutagenesis in aveD gene,and so as to provide theoretical basis for genetic breeding of S.avermitilis.[Method] PCR-driven overlap extension was conducted for the site-directed mutagenesis in aveD gene;the mutated aveD gene then was used to construct vector pDC3（pKC1139∷aveD） via molecular manipulations like in vitro enzyme digestion and ligation;the vector pDC3（pKC1139∷aveD） was then introduced to aveD deletion mutant 489 of avermectin-producing strain S.avermitilis 76-9.[Result] Mutant strain 536 of site-directed mutagenesis of S.avermitilis 76-9 was obtained by homologous recombination.The sequencing results show that the sixty-ninth base C in aveD-coding region of mutant 536 was substituted by T,and the corresponding amino acid Thr was mutated to be Ile.[Conclusion] This study laid basis for the development of strains specifically producing avermectin B.

High Performance 70nm CMOS Devices

A high performance 70nm CMOS device has been demonstrated for the first time in the continent, China. Some innovations in techniques are applied to restrain the short channel effect and improve the driving ability, such as 3nm nitrided oxide, dual poly Si gate electrode, novel super steep retrograde channel doping by heavy ion implantation, ultra shallow S/D extension formed by Ge PAI(Pre Amorphism Implantation) plus LEI(Low Energy Implantation), thin and low resistance Ti SALICIDE by Ge PAI and special cleaning, etc. The shortest channel length of the CMOS device is 70nm. The threshold voltages, G m and off current are 0 28V,490mS·mm -1 and 0 08nA/μm for NMOS and -0 3V,340mS·mm -1 and 0 2nA/μm for PMOS, respectively. Delays of 23 5ps/stage at 1 5V, 17 5ps/stage at 2 0V and 12 5ps/stage at 3V are achieved in the 57 stage unloaded 100nm CMOS ring oscillator circuits.

Nephelauxetic-Effect in DMS Zn_(1-x)Co_xSe and Effect of Pressure on Nephelauxetic-Effect

Based on the 3D electron's radial wave function of Co 2+ free ion,a Nephelauxetic effect modifying factor to modify the radial wave function is introduced when Co 2+ cations are put into the crystal field of Zn 1-x Co x Se.With the modified wave functions,the optical transitions for Zn 1-x Co x Se crystals are calculated.Moreover,based on the first principle of physics,the influences of high pressure to the Nephelauxetic effect modifying factor is considered,and the high pressure blue shift for the Zn 1-x Co x Se crystal absorption spectra are calculated and a shift rate of d E /d p =0 45meV/GPa is obtained.

Microstructural evolution of(TiAl)+Nb+W+B alloy

A newly designed TiAl alloy containing W,Nb,and B was produced through magnetic-flotation-melting method.Mass production of this TiAl-based alloy,15 kg ingot size,which is quite different from the 0.05 kg small ingot produced by arc-melting,has a large effect on the metallurgical properties,such as the grain size and the phase structures of the alloy.Heat treatments were carefully designed in order to reduce the amount of the high-temperature remaining β phase in the alloy,and to obtain optimal microstructures for mechanical behavior studies.A room-temperature ductility of 1.9% was obtained in the cast TiAl-based alloy after the appropriate heat treatment.The mechanical behavior of the large ingot through mass production of the TiAl-based alloy was largely improved by the alloy design and subsequent heat treatments.

Preparation and Detection of Extended DNA Fibers of Chinese Cabbage

Abstract [Objective] The paper was to prepare and detect the extended DNA fibers of Chinese cabbage. [Method] Chinese cabbage nuclei was first successfully isolated by chopping young leaves with a blade, then nuclei were lysed by SDS to release DNA, and DNA fibers were dragged and extended with a coverslip. [Result] The results of Fiber-FISH with genomic DNA and 25S rDNA as probes showed that DNA fiber size as long as about 1.93 Mb could be measured and the number of 25S rDNA copies region were estimated to be 258 and 687 in Chinese cabbage genome. DNA fibers prepared by this method showed equally spread parallel thread with clear background, and were suitable for FISH analysis. [Conclusion] The study would accelerate Chinese cabbage genome mapping and organization analysis.

The implementation of an improved NPML absorbing boundary condition in elastic wave modeling

In elastic wave forward modeling, absorbing boundary conditions （ABC） are used to mitigate undesired reflections from the model truncation boundaries. The perfectly matched layer （PML） has proved to be the best available ABC. However, the traditional splitting PML （SPML） ABC has some serious disadvantages： for example, global SPML ABCs require much more computing memory, although the implementation is easy. The implementation of local SPML ABCs also has some difficulties, since edges and corners must be considered. The traditional non-splitting perfectly matched layer （NPML） ABC has complex computation because of the convolution. In this paper, based on non-splitting perfectly matched layer （NPML） ABCs combined with the complex frequency-shifted stretching function （CFS）, we introduce a novel numerical implementation method for PML absorbing boundary conditions with simple calculation equations, small memory requirement, and easy programming.

Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers

Genes encoding enzymes involved in biosynthesis of very long chain fatty acids were significantly up-regulatedduring early cotton fiber development. Two cDNAs, GhKCR1 and GhKCR2 encoding putative cotton 3-ketoacyl-CoAreductases that catalyze the second step in fatty acid elongation, were isolated from developing cotton fibers. GhKCR1and 2 contain open reading frames of 963 bp and 924 bp encoding proteins of 320 and 307 amino acid residues,respectively. Quantatitive RT-PCR analysis showed that both these genes were highly preferentially expressed duringthe cotton fiber elongation period with much lower levels recovered from roots, stems and leaves. GhKCR1 and 2showed 30%-32% identity to Saccharomyces cerevisiae Ybr159p at the deduced amino acid level. These cotton cDNAswere cloned and expressed in yeast haploid ybr159w? mutant that was deficient in 3-ketoacyl-CoA reductase activity.Wild-type growth rate was restored in ybr159w? cells that expressed either GhKCR1 or 2. Further analysis showed thatGhKCR1 and 2 were co-sedimented within the membranous pellet fraction after high-speed centrifugation, similar to theyeast endoplasmic reticulum marker ScKar2p. Both GhKCR(s) showed NADPH-dependent 3-ketoacyl-CoA reductaseactivity in an in vitro assay system using palmitoyl-CoA and malonyl-CoA as substrates. Our results suggest thatGhKCR1 and 2 are functional orthologues of ScYbr159p.

New Periodic Wave Solutions to Generalized Klein-Gordon and Benjamin Equations

By making use of extended mapping method and auxiliary equation for finding new periodic wave solu tions of nonlinear evolution equations in mathematical physics, we obtain some new periodic wave solutions for generalized Klein-Cordon equation and Benjamin equation, which cannot be found in previous work. This method also can be used to find new periodic wave solutions of other nonlinear evolution equations.

Application of extension method to fault diagnosis of transformer

A novel extension diagnosis method was proposed for enhancing the diagnosis ability of the conventional dissolved gas analysis. Based on the extension theory a matter-element model was established for qualitatively and quantitatively describing the fault diagnosis problem of power transformers. The degree of relation based on the dependent functions was employed to determine the nature and the grade of the faults in a transformer system. And the proposed method was verified with the experimental data. The results show that accuracy rate of the diagnosis method exceeds 90% and two kinds of faults can be detected at the same time.

Modified Heisenberg Ferromagnet Model and Integrable Equation

We investigate some integrable modified Heisenberg ferromagnet models by using the prolongation structure theory. Through associating them with the motion of curve in Minkowski space, the corresponding coupled integrable equations are presented.

Prolongation Structure of Semi-discrete Nonlinear Evolution Equations

Based on noncommutative differential calculus, we present a theory of prolongation structure for semidiscrete non/inear evolution equations. As an illustrative example, a semi-discrete model of the non/inear SchrSdinger equation is discussed in terms of this theory and the corresponding Lax pairs are also given.

Near Crack Line Elastic-Plastic Analysis for an Infinite Plate Loaded by a Pair of Point Shear Forces

The near crack line analysis method was used to investigate a crack loaded by a pair of point shear forces in an infinite plate in an elastic-perfectly plastic solid. The analytical solution was obtained, that is the elastic-plastic fields near crack line and law that the length of the plastic zone along the crack line is varied with external loads. The results are sufficiently precise near the crack line and are not confined by small scale yielding conditions.

Analysis and behaviour of FRP-confined short concrete columns subjected to eccentric loading

Fibre-reinforced polymer (FRP) composites were widely utilized in civil engineering structures as the retrofit of reinforced concrete (RC) columns. To design FRP jackets safely and economically, the behaviour of such columns should be predicted first. This paper is concerned with the analysis and behaviour of FRP-confined RC circular and rectangular short col- umns subjected to eccentric loading. A simple design-oriented stress-strain model for FRP-confined concrete in a section analysis was first proposed. The accuracy was then proved by two test data. Following that, a parametric study including amount of FRP confinement, FRP strain capacity, unconfined concrete strength and shape of column section is provided. Some conclusions were obtained at the end of the paper. The work here will provide a comprehensive understanding of the behaviour of FRP-confined concrete columns. The simplicity of the model also enables a simple equivalent stress block to be developed for direct use in practical design.

Modeling effects of constituents and dispersoids on tensile ductility of aluminum alloy

The modeling effects of constituents and dispersoids on the tensile ductility of aluminum alloy were studied.The results show that the tensile ductility decreases with the increase of the volume fraction and size of constituents.Thus,purification can improve the tensile ductility by decreasing the volume fraction of constituents(normally compositions of Fe and Si)and the first-class microcracks.The model also indicates that the tensile ductility decreases with the increase in the volume fraction of dispersoids.Decreasing the volume fraction of dispersoids along the grain boundaries by proper heat-treatment and improving the cohesion strength between dispersoids and matrix can also improve the tensile ductility by decreasing the volume fraction of the second-class microcracks.

Agricultural Extension in Balochistan, Pakistan:Date Palm Farmers' Access and Satisfaction

This study analyzed information gathered from 200 date palm households in the mountainous Panjgur District of Balochistan in Pakistan with the aim of determining date farmers＇ access to and satisfaction with agricultural extension services. Information was collected through a structured questionnaire survey, group discussions and semi- structured personal interviews. The findings of the analysis revealed very poor access to extension services. The results of logistic regression analysis identified five variables out of six which significantly influence date farmers＇ access to extension services： household bead＇s age, household bead＇s literacy, number of date palm trees owned, mostly inherited date palm trees, and percentage of dead date palm trees. The overwhelming majority of farmers were dissatisfied with all three main types of extension services being provided by public extension officials. Explanations for this as well as for associations between access to extension services and the six independent variables are provided. Important policy conclusions are drawn based on the findings.

Periodic Semifolded Solitary Waves for (2+1)-Dimensional Variable Coefficient Broer-Kaup System

Applying the extended mapping method via Riccati equation, many exact variable separation solutions for the （2＆1 ）-dimensional variable coefficient Broer-Kaup equation are obtained. Introducing multiple valued function and Jacobi elliptic function in the seed solution, special types of periodic semifolded solitary waves are derived. In the long wave limit these periodic semifolded solitary wave excitations may degenerate into single semifolded localized soliton structures. The interactions of the periodic semifolded solitary waves and their degenerated single semifolded soliton structures are investigated graphically and found to be completely elastic.