Aqueous humor provides the necessary nutrition for the lens and transports the metabolites in the eye.It was a liquid that can directly reflect the microenvironment in the eye'and it can be easily obtained during ...Aqueous humor provides the necessary nutrition for the lens and transports the metabolites in the eye.It was a liquid that can directly reflect the microenvironment in the eye'and it can be easily obtained during the operation.This review intended to analyze the components of aqueous humor in patients with different types of cataracts'so as to reflect the pathogenesis and development of the disease'evaluate the incidence of postoperative complications and provide reference value for the surgical design of sequential cataract surgery.The aqueous humor components of different types of cataracts showed different degrees of inflammation'oxidative stress and extracellular matrix remodeling.The biomarker of early neuropathy in diabetic cataract was neural cell adhesion molecule-1(NCAM1).Transforming growth factor-β(TGF-β)was the evaluation factor of disease development in patients with pseudoexfoliation syndrome.The relationships between postoperative complications of different types of cataracts and aqueous humor components were as follows:Macular edema after diabetic cataract surgery was associated with tumor necrosis factor-alpha;capsular contraction after high myopic cataract surgery was related to monocyte chemoattractant protein-1(MCP-1)and TGF-β2;Klotho and glutathione S-transferase P 1(GSTP1)were associated with high intraocular pressure after primary open-angle glaucoma complicated by cataract surgery;capsular contraction after retinitis pigmentosa complicated by cataract surgery was associated with matrix metalloproteinases;pro-inflammatory cytokines and fibroblast growth factor 4 in the aqueous humor of congenital cataracts were associated with posterior capsular opacification after surgery.Granulocyte colony stimulating factor 3 and MCP-1 were the main cytokines mediating the pain of the second eye in the binocular sequential cataract surgery short interval(1 wk)'while MCP-1 mediated pain in the long interval(6 wk).The second eye after binocular sequential cataract surgery had a higher level of proinflammatory factors.The components of aqueous humor in patients with different types of cataracts were related to the pathogenesis and postoperative complications of the disease.Monitoring the components of the aqueous humor could help better understand the intraocular microenvironment of different types of cataracts and provide a reference for predicting the development of the disease and implementing relevant targeted therapy.展开更多
Toll-like receptor 4 （TLR4） recognizes pathogen ligands and mediates signaling to initiate innate and adaptive immune responses. In this experiment, a 316 bp and 382 bp fragments of TLR4 gene named T4CRBR1 and T4CRB...Toll-like receptor 4 （TLR4） recognizes pathogen ligands and mediates signaling to initiate innate and adaptive immune responses. In this experiment, a 316 bp and 382 bp fragments of TLR4 gene named T4CRBR1 and T4CRBR2, of Chinese Holstein, Sanhe cattle, and Chinese Simmental was amplified by polymerase chain reaction （PCR）, respectively. The genetic polymorphisms in the three populations were detected by Single-Strand Conformational Polymorphism （SSCP） in the first locus and by digesting the fragments with restriction endonuclease Alu I in the second one. Results showed that both alleles （A and B） of two loci were found in all the three populations and the value of polymorphism information content （PIC） indicated that these were a moderate polymorphism. Statistical results of X^2 test indicated that two polymorphism sites in the three populations fitted with Hardy-Weinberg equilibrium （P 〉 0.05）. After sequencing, A-G single nucleotide polymorphism （SNP） was identified at nucleotide 4,525 in intron 1 of TLR4 gene and C-T SNP was identified at nucleotide 1,397 in exon 3 of TLR4 gene. Meanwhile, the effect of polymorphism of TLR4 gene on somatic cell score （SCS） was analyzed, the results indicated that the cattle with allele A in T4CRBR1 showed lower somatic cell score than that of allele B （P 〈 0.05）. In short, the allele A might play an important role in mastiffs resistance in bovine.展开更多
According to the actual conditions in Sanshandao Gold Mine, the cancelling ore pillars mining method was researched. Firstly, a series of tests for the physical and mechanical characteristics of rock mass were carried...According to the actual conditions in Sanshandao Gold Mine, the cancelling ore pillars mining method was researched. Firstly, a series of tests for the physical and mechanical characteristics of rock mass were carried out and a quality classification system of rock mass applied in coastal metal deposit was established. Secondly, the reasonable demarcation depth of cancelling ore pillars was simulated using the finite element method, and the simulation results show that the ore pillars can be cancelled below the level of-555 m. Thirdly, a novel layer-backfill mining method of room-pillar alternation was designed to reduce the disturbance and settlement of terrain in mining area. Engineering practice shows that the new mining method enhanced the mining output and relieved rock disturbance. Furthermore, the settlement of the roof strata was small and no disaster occurred. The new mining technology effectively controlled the deformation of the terrain, indicating that the mining of the large-scale gold coastal deposit in Sanshandao Gold Mine was achieved safely, efficiently, and with a low loss rate.展开更多
Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in ...Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in Daohugou village, Shantou town, Ningcheng County, Nei Mongol Autonomous Region, China. The stem of plant is slender and repeatedly branches as a dichasial system with a fertile shoot in the fork, forming equal and widely divergent branches, on the branches born with the fronds of Anomozamites type. Its reproductive organs consists of some microsporophylls and a few of the bracteoid small leaves (or receptacular leaves). Some scattered bracteoid leaves and microsporophylls were formerly considered by Pan as dicots. Since then, they were proposed to be a non-committal genus Pankuangia and described them as P. haifanggouensis by Kimura et al. In this study, we discover that these bracteoid small leaves and microsporophylls should be of Anomozamites. This discovery provides strong evidence for the 'Pankuangia' determination of the taxonomical position.展开更多
The research selected 13 factors from individual and family characters, construction site characters and work characters and analyzed the influences on the willingness of purchasing houses on construction sites with L...The research selected 13 factors from individual and family characters, construction site characters and work characters and analyzed the influences on the willingness of purchasing houses on construction sites with Logistic regression mod- el. The results showed that gender, age differences, personality, willingness on fami- ly land conversion, management of enterprises on construction sites, relatives and friends on construction lands, and Job satisfaction were approved by significant testing, but marital status, annual household income, the rate of migrant worker in a household, transportation in work city, and corporate reputation were not approved by significant testing.展开更多
Objective: To evaluate of the role of transesophageal echocardiography (TEE)in percutaneous closure of atrial septal defects (ASD) with the Amplatzer septal occluder. Methods:Sixty- two patients (10 to 55 years of age...Objective: To evaluate of the role of transesophageal echocardiography (TEE)in percutaneous closure of atrial septal defects (ASD) with the Amplatzer septal occluder. Methods:Sixty- two patients (10 to 55 years of age) were selected for percutaneous closure of ASD bytrans-esophageal echocardiography, which was also used to monitor the procedure, to select theappropriate size of the Amplatzer device, to verify its position, and to access the immediateresults of the procedure. During the follow-up, transthoracic echocardiography (TTE) or TEE was usedto evaluate the presence and magnitude of residual shunt (RS), device position, and right cardiacchamber diameters. Results: The mean ASD diameter by TTE ([19. 1 +- 5. 8] mm) was significantlysmaller (P< 0. 001) than the stretched diameter of the ASD (25. 1 +- 6. 4) mm. There are nosignificant differences between the TEE -measured value (23. 5+_6. 2) mm and the stretched diameterof the ASD (P > 0. 05). Due to proper patient selection all procedures were successful. There wasimmediate and complete closure in 61/62 patients, only one patients had trivial residual shunt.Follow- up was performed using TTE or TEE right after operation, 1 d, 1 month, 3 months, 6 monthsand yearly thereafter. Ail, patients remain asymptomatic without any clinical or technical problems.Conclusion: With the aid of TEE, percutaneous closure of ASD can be performed successfully, safely,and effectively.展开更多
[Objective] The aim was to investigate ecological carrying capacity of Fangshan District in Beijing. [Method] Ecological footprint and carrying capacity of Fangshan District in 2007 and 2008 were analyzed with ecologi...[Objective] The aim was to investigate ecological carrying capacity of Fangshan District in Beijing. [Method] Ecological footprint and carrying capacity of Fangshan District in 2007 and 2008 were analyzed with ecological footprint model and the difference between ecological footprint and carrying capacity there was con- cluded. Furthermore, an assessment was made on relation between Fangshan de- velopment with local ecological carrying capacity to evaluate sustainable development of Fangshan District. [Result] Fangshan District is of lightly ecological deficit; ecologi- cal footprint per capita in 2007-2008 was deficit and tended to expand. Two meth- ods are available to reduce ecological ：deficit, including reduction of ecological foot- print and increase of carrying capacity. In general, supply and demand of carrying capacity in Fangshan are better, though with some problems. It is necessary to fur- ther develop related industries to final eliminate ecological deficit in order to achieve ecological conservation-orientation and sustainable development of Fangshan District. [Conclusion] The research provides theoretical references for overall evaluation on eco- logical carrying capacity of Fangshan District.展开更多
This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has bee...This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.展开更多
文摘Aqueous humor provides the necessary nutrition for the lens and transports the metabolites in the eye.It was a liquid that can directly reflect the microenvironment in the eye'and it can be easily obtained during the operation.This review intended to analyze the components of aqueous humor in patients with different types of cataracts'so as to reflect the pathogenesis and development of the disease'evaluate the incidence of postoperative complications and provide reference value for the surgical design of sequential cataract surgery.The aqueous humor components of different types of cataracts showed different degrees of inflammation'oxidative stress and extracellular matrix remodeling.The biomarker of early neuropathy in diabetic cataract was neural cell adhesion molecule-1(NCAM1).Transforming growth factor-β(TGF-β)was the evaluation factor of disease development in patients with pseudoexfoliation syndrome.The relationships between postoperative complications of different types of cataracts and aqueous humor components were as follows:Macular edema after diabetic cataract surgery was associated with tumor necrosis factor-alpha;capsular contraction after high myopic cataract surgery was related to monocyte chemoattractant protein-1(MCP-1)and TGF-β2;Klotho and glutathione S-transferase P 1(GSTP1)were associated with high intraocular pressure after primary open-angle glaucoma complicated by cataract surgery;capsular contraction after retinitis pigmentosa complicated by cataract surgery was associated with matrix metalloproteinases;pro-inflammatory cytokines and fibroblast growth factor 4 in the aqueous humor of congenital cataracts were associated with posterior capsular opacification after surgery.Granulocyte colony stimulating factor 3 and MCP-1 were the main cytokines mediating the pain of the second eye in the binocular sequential cataract surgery short interval(1 wk)'while MCP-1 mediated pain in the long interval(6 wk).The second eye after binocular sequential cataract surgery had a higher level of proinflammatory factors.The components of aqueous humor in patients with different types of cataracts were related to the pathogenesis and postoperative complications of the disease.Monitoring the components of the aqueous humor could help better understand the intraocular microenvironment of different types of cataracts and provide a reference for predicting the development of the disease and implementing relevant targeted therapy.
基金This work was supported by the fifteenth "Five Years Key Programs" for Science and Technology Development of China (No. 2002BA518A14)the National High Science and Technology Foundation of China (863) (No. 2002AA242011).
文摘Toll-like receptor 4 （TLR4） recognizes pathogen ligands and mediates signaling to initiate innate and adaptive immune responses. In this experiment, a 316 bp and 382 bp fragments of TLR4 gene named T4CRBR1 and T4CRBR2, of Chinese Holstein, Sanhe cattle, and Chinese Simmental was amplified by polymerase chain reaction （PCR）, respectively. The genetic polymorphisms in the three populations were detected by Single-Strand Conformational Polymorphism （SSCP） in the first locus and by digesting the fragments with restriction endonuclease Alu I in the second one. Results showed that both alleles （A and B） of two loci were found in all the three populations and the value of polymorphism information content （PIC） indicated that these were a moderate polymorphism. Statistical results of X^2 test indicated that two polymorphism sites in the three populations fitted with Hardy-Weinberg equilibrium （P 〉 0.05）. After sequencing, A-G single nucleotide polymorphism （SNP） was identified at nucleotide 4,525 in intron 1 of TLR4 gene and C-T SNP was identified at nucleotide 1,397 in exon 3 of TLR4 gene. Meanwhile, the effect of polymorphism of TLR4 gene on somatic cell score （SCS） was analyzed, the results indicated that the cattle with allele A in T4CRBR1 showed lower somatic cell score than that of allele B （P 〈 0.05）. In short, the allele A might play an important role in mastiffs resistance in bovine.
基金Projects(2013BAB02B03,2012BAB08B00)supported by the National Science and Technology Support Program of ChinaProject(51074177)supported by the Joint Funding of National Natural Science Foundation and Shanghai Baosteel Group CorporationProjects(51274254,51322403)supported by the National Natural Science Foundation of China
文摘According to the actual conditions in Sanshandao Gold Mine, the cancelling ore pillars mining method was researched. Firstly, a series of tests for the physical and mechanical characteristics of rock mass were carried out and a quality classification system of rock mass applied in coastal metal deposit was established. Secondly, the reasonable demarcation depth of cancelling ore pillars was simulated using the finite element method, and the simulation results show that the ore pillars can be cancelled below the level of-555 m. Thirdly, a novel layer-backfill mining method of room-pillar alternation was designed to reduce the disturbance and settlement of terrain in mining area. Engineering practice shows that the new mining method enhanced the mining output and relieved rock disturbance. Furthermore, the settlement of the roof strata was small and no disaster occurred. The new mining technology effectively controlled the deformation of the terrain, indicating that the mining of the large-scale gold coastal deposit in Sanshandao Gold Mine was achieved safely, efficiently, and with a low loss rate.
文摘Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in Daohugou village, Shantou town, Ningcheng County, Nei Mongol Autonomous Region, China. The stem of plant is slender and repeatedly branches as a dichasial system with a fertile shoot in the fork, forming equal and widely divergent branches, on the branches born with the fronds of Anomozamites type. Its reproductive organs consists of some microsporophylls and a few of the bracteoid small leaves (or receptacular leaves). Some scattered bracteoid leaves and microsporophylls were formerly considered by Pan as dicots. Since then, they were proposed to be a non-committal genus Pankuangia and described them as P. haifanggouensis by Kimura et al. In this study, we discover that these bracteoid small leaves and microsporophylls should be of Anomozamites. This discovery provides strong evidence for the 'Pankuangia' determination of the taxonomical position.
基金Supported by National Natural Science Foundation of China(71363027)Humanities and Social Sciences of Ministry of Education(11YJA790088)Jiangxi Humanities and Social Sciences(10YJ17)~~
文摘The research selected 13 factors from individual and family characters, construction site characters and work characters and analyzed the influences on the willingness of purchasing houses on construction sites with Logistic regression mod- el. The results showed that gender, age differences, personality, willingness on fami- ly land conversion, management of enterprises on construction sites, relatives and friends on construction lands, and Job satisfaction were approved by significant testing, but marital status, annual household income, the rate of migrant worker in a household, transportation in work city, and corporate reputation were not approved by significant testing.
文摘Objective: To evaluate of the role of transesophageal echocardiography (TEE)in percutaneous closure of atrial septal defects (ASD) with the Amplatzer septal occluder. Methods:Sixty- two patients (10 to 55 years of age) were selected for percutaneous closure of ASD bytrans-esophageal echocardiography, which was also used to monitor the procedure, to select theappropriate size of the Amplatzer device, to verify its position, and to access the immediateresults of the procedure. During the follow-up, transthoracic echocardiography (TTE) or TEE was usedto evaluate the presence and magnitude of residual shunt (RS), device position, and right cardiacchamber diameters. Results: The mean ASD diameter by TTE ([19. 1 +- 5. 8] mm) was significantlysmaller (P< 0. 001) than the stretched diameter of the ASD (25. 1 +- 6. 4) mm. There are nosignificant differences between the TEE -measured value (23. 5+_6. 2) mm and the stretched diameterof the ASD (P > 0. 05). Due to proper patient selection all procedures were successful. There wasimmediate and complete closure in 61/62 patients, only one patients had trivial residual shunt.Follow- up was performed using TTE or TEE right after operation, 1 d, 1 month, 3 months, 6 monthsand yearly thereafter. Ail, patients remain asymptomatic without any clinical or technical problems.Conclusion: With the aid of TEE, percutaneous closure of ASD can be performed successfully, safely,and effectively.
文摘[Objective] The aim was to investigate ecological carrying capacity of Fangshan District in Beijing. [Method] Ecological footprint and carrying capacity of Fangshan District in 2007 and 2008 were analyzed with ecological footprint model and the difference between ecological footprint and carrying capacity there was con- cluded. Furthermore, an assessment was made on relation between Fangshan de- velopment with local ecological carrying capacity to evaluate sustainable development of Fangshan District. [Result] Fangshan District is of lightly ecological deficit; ecologi- cal footprint per capita in 2007-2008 was deficit and tended to expand. Two meth- ods are available to reduce ecological ：deficit, including reduction of ecological foot- print and increase of carrying capacity. In general, supply and demand of carrying capacity in Fangshan are better, though with some problems. It is necessary to fur- ther develop related industries to final eliminate ecological deficit in order to achieve ecological conservation-orientation and sustainable development of Fangshan District. [Conclusion] The research provides theoretical references for overall evaluation on eco- logical carrying capacity of Fangshan District.
文摘This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.
