The SOX2 protein is an important transcription factor functioning during the early development of animals. In this study, we isolated a full-length c DNA sequence of scallop Chlamys farreri sox2, Cf-sox2 which was 219...The SOX2 protein is an important transcription factor functioning during the early development of animals. In this study, we isolated a full-length c DNA sequence of scallop Chlamys farreri sox2, Cf-sox2 which was 2194 bp in length with a 981 bp open reading frame encoding 327 amino acids. With real-time PCR analysis, it was detected that Cf-sox2 was expressed in unfertilized oocytes, fertilized eggs and all the tested embryos and larvae. The expression level increased significantly(P < 0.01) in embryos from 2-cell to blastula, and then decreased significantly(P < 0.01) and reached the minimum in umbo larva. Moreover, location of the Cfsox2 expression was revealed using whole mount in situ hybridization technique. Positive hybridization signal could be detected in the central region of unfertilized oocytes and fertilized eggs, and then strong signals dispersed throughout the embryos from 2-cell to gastrula. During larval development, the signals were concentrated and strong signals were restricted to 4 regions of viscera mass in veliger larva. In umbo larva, weak signals could be detected in regions where presumptive visceral and pedal ganglia may be formed. The expression pattern of Cf-sox2 during embryogenesis was similar to that of mammal sox2, which implied that Cf-SOX2 may participate in the regulation of early development of C. farreri.展开更多
The unique Coanda surface has a great influence on the performance of bladeless fan.However,there is few studies to explain the relationship between the performance and Coanda surface curvature at present.In order to ...The unique Coanda surface has a great influence on the performance of bladeless fan.However,there is few studies to explain the relationship between the performance and Coanda surface curvature at present.In order to gain a qualitative understanding of effect of the curvature on the performance of bladeless fan,numerical studies are performed in this paper.Firstly,three-dimensional numerical simulation is done by Fluent software.For the purpose to obtain detailed information of the flow field around the Coanda surface,two-dimensional numerical simulation is also conducted.Five types of Coanda surfaces with different curvature are designed,and the flow behaviour and the performance of them are analyzed and compared with those of the prototype.The analysis indicates that the curvature of Coanda surface is strongly related to blowing performance,It is found that there is an optimal curvature of Coanda surfaces among the studied models.Simulation result shows that there is a special low pressure region.With increasing curvature in Y direction,several low pressure regions gradually enlarged,then begin to merge slowly,and finally form a large area of low pressure.From the analyses of streamlines and velocity angle,it is found that the magnitude of the curvature affects the flow direction and reasonable curvature can induce fluid flow close to the wall.Thus,it leads to that the curvature of the streamlines is consistent with that of Coanda surface.Meanwhile,it also causes the fluid movement towards the most suitable direction.This study will provide useful information to performance improvements of bladeless fans.展开更多
This paper deals with the qualitative behavior of orbits at degenerate singular point with the method of quasi normal sector, which is a generalization of Frommer's normal sectors. Several examples show that this ...This paper deals with the qualitative behavior of orbits at degenerate singular point with the method of quasi normal sector, which is a generalization of Frommer's normal sectors. Several examples show that this method is more effective than the wellknown methods of Z-sectors, normal sectors and generalized normal sector.展开更多
A novel approach of iso-scallop trajectory generation for smooth manifold surfaces has been developed. Firstly,by defining homeomorphism mapping relations and differentiable structures,the smooth manifold surface is m...A novel approach of iso-scallop trajectory generation for smooth manifold surfaces has been developed. Firstly,by defining homeomorphism mapping relations and differentiable structures,the smooth manifold surface is mapped into several Euclidean planes,thus its trajectory generation can be decomposed into planar curve-filling tasks. Secondly,in the generation of direction-parallel trajectories,the calculation of the cutting interval and the curvature is given,depending on the generation of the first curve in the projection view. Thirdly,after automatic adherences of inverse projection curves,the filled curves are mapped into the original surface inversely to form trajectories. Although the required trajectories are iso-scallop,the trajectory intervals are variable according to the curvature changes at the projection point,which is advantageous to improving the trajectory quality. The proposed approach has appealing merits of dimensionality reduction,which decreases the algorithm complexity. Finally,numerical and machining examples are given to illustrate its feasibility and validity.展开更多
基金supported by the National High Technology Research and Development Program of China(863 Program)(2012AA10A402)
文摘The SOX2 protein is an important transcription factor functioning during the early development of animals. In this study, we isolated a full-length c DNA sequence of scallop Chlamys farreri sox2, Cf-sox2 which was 2194 bp in length with a 981 bp open reading frame encoding 327 amino acids. With real-time PCR analysis, it was detected that Cf-sox2 was expressed in unfertilized oocytes, fertilized eggs and all the tested embryos and larvae. The expression level increased significantly(P < 0.01) in embryos from 2-cell to blastula, and then decreased significantly(P < 0.01) and reached the minimum in umbo larva. Moreover, location of the Cfsox2 expression was revealed using whole mount in situ hybridization technique. Positive hybridization signal could be detected in the central region of unfertilized oocytes and fertilized eggs, and then strong signals dispersed throughout the embryos from 2-cell to gastrula. During larval development, the signals were concentrated and strong signals were restricted to 4 regions of viscera mass in veliger larva. In umbo larva, weak signals could be detected in regions where presumptive visceral and pedal ganglia may be formed. The expression pattern of Cf-sox2 during embryogenesis was similar to that of mammal sox2, which implied that Cf-SOX2 may participate in the regulation of early development of C. farreri.
基金supported by National Natural Science Foundation of China(No.51276172)National Science and TechnologySupport Project(No.2013BAF05B01)
文摘The unique Coanda surface has a great influence on the performance of bladeless fan.However,there is few studies to explain the relationship between the performance and Coanda surface curvature at present.In order to gain a qualitative understanding of effect of the curvature on the performance of bladeless fan,numerical studies are performed in this paper.Firstly,three-dimensional numerical simulation is done by Fluent software.For the purpose to obtain detailed information of the flow field around the Coanda surface,two-dimensional numerical simulation is also conducted.Five types of Coanda surfaces with different curvature are designed,and the flow behaviour and the performance of them are analyzed and compared with those of the prototype.The analysis indicates that the curvature of Coanda surface is strongly related to blowing performance,It is found that there is an optimal curvature of Coanda surfaces among the studied models.Simulation result shows that there is a special low pressure region.With increasing curvature in Y direction,several low pressure regions gradually enlarged,then begin to merge slowly,and finally form a large area of low pressure.From the analyses of streamlines and velocity angle,it is found that the magnitude of the curvature affects the flow direction and reasonable curvature can induce fluid flow close to the wall.Thus,it leads to that the curvature of the streamlines is consistent with that of Coanda surface.Meanwhile,it also causes the fluid movement towards the most suitable direction.This study will provide useful information to performance improvements of bladeless fans.
基金supported by the National Natural Science Foundation of China(No.11401111,No.11171355)the Ph.D.Programs Foundation of Ministry of Education of China(No.20100171110040)
文摘This paper deals with the qualitative behavior of orbits at degenerate singular point with the method of quasi normal sector, which is a generalization of Frommer's normal sectors. Several examples show that this method is more effective than the wellknown methods of Z-sectors, normal sectors and generalized normal sector.
基金supported by the National Natural Science Foundation of China (Grant Nos.50835004,50905131)the Natural Science Foundation of Hubei Province (Grant No.2009CDB251)
文摘A novel approach of iso-scallop trajectory generation for smooth manifold surfaces has been developed. Firstly,by defining homeomorphism mapping relations and differentiable structures,the smooth manifold surface is mapped into several Euclidean planes,thus its trajectory generation can be decomposed into planar curve-filling tasks. Secondly,in the generation of direction-parallel trajectories,the calculation of the cutting interval and the curvature is given,depending on the generation of the first curve in the projection view. Thirdly,after automatic adherences of inverse projection curves,the filled curves are mapped into the original surface inversely to form trajectories. Although the required trajectories are iso-scallop,the trajectory intervals are variable according to the curvature changes at the projection point,which is advantageous to improving the trajectory quality. The proposed approach has appealing merits of dimensionality reduction,which decreases the algorithm complexity. Finally,numerical and machining examples are given to illustrate its feasibility and validity.