To investigate the mutations in the upstream regulatory region (URR) of human papillomavirus type 16 (HPV-16) from the cervical cancer biopsies in Xinjiang Uygur women and its relationship to the high incidence of cer...To investigate the mutations in the upstream regulatory region (URR) of human papillomavirus type 16 (HPV-16) from the cervical cancer biopsies in Xinjiang Uygur women and its relationship to the high incidence of cervical cancer in the southern Xinjiang, the tissue DNA was extracted from the cervical cancer biopsies, and the URR segment of HPV-16 DNA was amplified, sequenced and analyzed. Thereafter, the polymorphism of URR in HPV-16 was then analyzed. It was demonstrated that the positive rate detected for the presence of URR in HPV-16 was 89.47% (17/19). Compared with the previously published sequence in URR of prototype HPV-16, some mutations were detected in the sequence of URR. The mutations in 17 URR fragments of HPV-16 could be divided into 11 patterns (XJU-1 to XJU-11) at nucleic acid level, in which each of XJU-1 and XJU-4 accounted for 23.53% (4/17), and other patterns of mutation accounted for 5.88% (1/17) . In comparison with the URR of prototype HPV-16, the DNA identity of these patterns was 98.50%-99.68% . In these 17 URR fragments, two point mutations occurred at position 7192 (G to T) and position 7520 (G to A) and they appeared to be constant in Xinjiang area. These two mutations were ubiquitous in the Asia-American type and conferred strong infection activity and carcinogenicity of this virus. In addition, the mutations at position 7729 (A to C), position 7843 (A to G) and position 7792 (C to T) could enhance its transcription activity considerably. It is concluded that some mutations occur in URR gene of HPV-16 in the cervical cancer biopsies taken from Uygur women in Xinjiang area, suggesting that certain relationship exists among the mutations in URR of HPV-16, the phylogeny of HPV-16 and the high incidence of cervical cancer in southern part of Xinjiang area.展开更多
The Chars Livelihoods Programme （CLP）＇s response has been to provide a comprehensive package of support, which aims to increase resilience to disasters and build women＇s empowerment. In 2012, CLP developed an inno...The Chars Livelihoods Programme （CLP）＇s response has been to provide a comprehensive package of support, which aims to increase resilience to disasters and build women＇s empowerment. In 2012, CLP developed an innovative approach to monitor women＇s empowerment. Understanding that empowerment is context specific, therefore CLP used qualitative approaches to understand how char households define empowerment. From this research, CLP developed a set of 10 （community and household level） indicators, which made up the chars empowerment scorecard. Results show that CLP has a considerable impact on women＇s empowennent. They also show that CLP has impacts at both the household and community level, driven by two main factors： increased knowledge （through social development training） and increased wealth （through CLP＇s Asset Transfer Project）. Further, impacts on empowerment are seen to occur relatively quickly and sustain over time. The effects of climate change are only likely to further compound problems for char-dwellers. By increasing women＇s empowerment, CLP is confident that they will be better able to build sustainable livelihoods and increase their resilience to future disasters.展开更多
基金grants from the National Natural Science Foundation of China （No. 30460008） .
文摘To investigate the mutations in the upstream regulatory region (URR) of human papillomavirus type 16 (HPV-16) from the cervical cancer biopsies in Xinjiang Uygur women and its relationship to the high incidence of cervical cancer in the southern Xinjiang, the tissue DNA was extracted from the cervical cancer biopsies, and the URR segment of HPV-16 DNA was amplified, sequenced and analyzed. Thereafter, the polymorphism of URR in HPV-16 was then analyzed. It was demonstrated that the positive rate detected for the presence of URR in HPV-16 was 89.47% (17/19). Compared with the previously published sequence in URR of prototype HPV-16, some mutations were detected in the sequence of URR. The mutations in 17 URR fragments of HPV-16 could be divided into 11 patterns (XJU-1 to XJU-11) at nucleic acid level, in which each of XJU-1 and XJU-4 accounted for 23.53% (4/17), and other patterns of mutation accounted for 5.88% (1/17) . In comparison with the URR of prototype HPV-16, the DNA identity of these patterns was 98.50%-99.68% . In these 17 URR fragments, two point mutations occurred at position 7192 (G to T) and position 7520 (G to A) and they appeared to be constant in Xinjiang area. These two mutations were ubiquitous in the Asia-American type and conferred strong infection activity and carcinogenicity of this virus. In addition, the mutations at position 7729 (A to C), position 7843 (A to G) and position 7792 (C to T) could enhance its transcription activity considerably. It is concluded that some mutations occur in URR gene of HPV-16 in the cervical cancer biopsies taken from Uygur women in Xinjiang area, suggesting that certain relationship exists among the mutations in URR of HPV-16, the phylogeny of HPV-16 and the high incidence of cervical cancer in southern part of Xinjiang area.
文摘The Chars Livelihoods Programme （CLP）＇s response has been to provide a comprehensive package of support, which aims to increase resilience to disasters and build women＇s empowerment. In 2012, CLP developed an innovative approach to monitor women＇s empowerment. Understanding that empowerment is context specific, therefore CLP used qualitative approaches to understand how char households define empowerment. From this research, CLP developed a set of 10 （community and household level） indicators, which made up the chars empowerment scorecard. Results show that CLP has a considerable impact on women＇s empowennent. They also show that CLP has impacts at both the household and community level, driven by two main factors： increased knowledge （through social development training） and increased wealth （through CLP＇s Asset Transfer Project）. Further, impacts on empowerment are seen to occur relatively quickly and sustain over time. The effects of climate change are only likely to further compound problems for char-dwellers. By increasing women＇s empowerment, CLP is confident that they will be better able to build sustainable livelihoods and increase their resilience to future disasters.
