Follistatin （Fst） is a hyperplasia factor that plays a crucial role in muscle development. DNA methylation, a significant process, regulates gene expression. The aim of our study is to examine the DNA methylation a...Follistatin （Fst） is a hyperplasia factor that plays a crucial role in muscle development. DNA methylation, a significant process, regulates gene expression. The aim of our study is to examine the DNA methylation and expression patterns ofFst gene at five different development stages of Japanese flounder （stage A, 7 dph; stage B, 90 dph; stage C, about 180 dph; stage D, about 24 months; stage E, about 36 months）. The muscle tissue of Japanese flounder was obtained at different development stages in dais experiment. DNA methylation levels in the promoter and exon 2 of Fst were determined by bisulfite sequencing, and the relative expression of the Fst gene at the five stages was measured by quantitative PCR. The results showed that the lowest methylation level was at stage A and the highest methylation level was at stage B. Moreover, the highest expression level of the Fst gene was observed at stage A. The mRNA abundance was negatively correlated with DNA methylation level. Three CpG islands in the promoter region and three CpG islands in exon 2 of Fst were found in the binding sequence of the putative transcription factor. These results offered a theoretical basis for the mechanism of Fst gene regulation to muscle development at different development stages.展开更多
Authenticity is an important issue in language testing. Business English （BE） assessment should focus more on authenticity in order to reflect the characteristics and changing teaching methods of BE. This paper expl...Authenticity is an important issue in language testing. Business English （BE） assessment should focus more on authenticity in order to reflect the characteristics and changing teaching methods of BE. This paper explores the idea of authenticity and uses real examples to show how to set criteria, and how to design authentic tasks to achieve authentic BE assessment.展开更多
基金supported by Natural Science Foundation of Shandong Province,China(No.ZR2014CM018)the National Natural Science Foundation of China(No.31672642)the AoShan Talents Program Supported by Qingdao National Laboratory for Marine Science and Technology(No.2017ASTCP-ES06)
文摘Follistatin （Fst） is a hyperplasia factor that plays a crucial role in muscle development. DNA methylation, a significant process, regulates gene expression. The aim of our study is to examine the DNA methylation and expression patterns ofFst gene at five different development stages of Japanese flounder （stage A, 7 dph; stage B, 90 dph; stage C, about 180 dph; stage D, about 24 months; stage E, about 36 months）. The muscle tissue of Japanese flounder was obtained at different development stages in dais experiment. DNA methylation levels in the promoter and exon 2 of Fst were determined by bisulfite sequencing, and the relative expression of the Fst gene at the five stages was measured by quantitative PCR. The results showed that the lowest methylation level was at stage A and the highest methylation level was at stage B. Moreover, the highest expression level of the Fst gene was observed at stage A. The mRNA abundance was negatively correlated with DNA methylation level. Three CpG islands in the promoter region and three CpG islands in exon 2 of Fst were found in the binding sequence of the putative transcription factor. These results offered a theoretical basis for the mechanism of Fst gene regulation to muscle development at different development stages.
文摘Authenticity is an important issue in language testing. Business English （BE） assessment should focus more on authenticity in order to reflect the characteristics and changing teaching methods of BE. This paper explores the idea of authenticity and uses real examples to show how to set criteria, and how to design authentic tasks to achieve authentic BE assessment.
