Four wood rot fungi were used to decay 300 mature trees of white birch in Liangshui experiment stations,and the mass loss of the samples was measured to study bio-degradability of the wood by the fungi. The decaying s...Four wood rot fungi were used to decay 300 mature trees of white birch in Liangshui experiment stations,and the mass loss of the samples was measured to study bio-degradability of the wood by the fungi. The decaying susceptible and resistant populations in 10 trees each with the highest and lowest mass loss were selected,in which some main chemical components in decayed and non-decayed wood such as lignin and cellulose and so on was tested. The result showed that the decomposing power of Coriolus versicolor to wood samples was better,and then were Fomes fomentarius,Irpex lacteus and Pholiota adiposa were poor. 1%NaOH extraction in decayed wood was significant higher than that in non-decayed control. Benzene-alcohol extraction in the wood decayed by Coriolus versicolor and Fomes fomentarius was significant higher than that in non-decayed control,but much lower than the control in the wood decayed by Irpex lacteus. The lignin decomposing rate by Irpex lacteus,Pholiota adipose and Fomes fomentarius were faster than the cellulose decomposing rate,the contrary result was achieved by Coriolus versicolor. This paper would provide some useful information in future study in the course and mechanism of wood decaying by rot fungi,and select engineering fungi for paper-making industry and pollution control.展开更多
Five wood rot fungi were used to decay 300 mature trees of white birch(Betula platyphylla)in Liangshui experiment stations.The decaying susceptible and resistant populations in 10 trees each with the highest and lowes...Five wood rot fungi were used to decay 300 mature trees of white birch(Betula platyphylla)in Liangshui experiment stations.The decaying susceptible and resistant populations in 10 trees each with the highest and lowest weight loss were selected,in which total phenol in wood was tested.The result showed that the birch wood with lower total phenol was easy to be decayed by Fomes fomentarius and Piptoporus betulinus;Irpex lacteus,Pholiota adiposa and Coriolus versicolor were not sensitive to total phenol in white birch wood.After GC-MS test to methanol extractionof white birch wood,6 organic compounds were found.This paper would provide some useful information in future study difference in the chemical characteristics of the wood between decaying susceptible and resistant populations,and select natural anti-decadent plants.展开更多
木腐真菌是一类木质纤维素的自然分解者,在生态系统的物质循环中发挥着重要角色。利用真菌子实体、菌落、菌丝体和分生孢子形态,结合内转录间隔区(ITS)和26S r DNA D1/D2区域序列,对环境中采集到的木腐真菌进行鉴定,并对分离鉴定到的真...木腐真菌是一类木质纤维素的自然分解者,在生态系统的物质循环中发挥着重要角色。利用真菌子实体、菌落、菌丝体和分生孢子形态,结合内转录间隔区(ITS)和26S r DNA D1/D2区域序列,对环境中采集到的木腐真菌进行鉴定,并对分离鉴定到的真菌进行木质素酶和纤维素酶活性分析,以及木材侵染腐朽能力研究。通过鉴定,共分离得到5种木腐真菌,分别是尖孢镰刀菌Fusarium oxysporum,毛栓孔菌Trametes hirsuta,层生镰刀菌Fusarium proliferatum,裂褶菌Schizophyllum commune和血红密孔菌Pycnoporus sanguineus。酶活分析表明:5种真菌均表现出较高的漆酶和锰过氧化物酶活性,但纤维素酶活性则普遍较低或不存在。利用质量损失法分析5种真菌对6种不同木材样品的生物降解能力,12周腐朽实验结果表明:毛栓孔菌的木材腐朽能力较强,造成对橡木Quercus mongolica最高的69.16%的质量损失率,而尖孢镰刀菌、层生镰刀菌和裂褶菌等的木材腐朽能力较弱,造成木材质量损失率普遍低于3.00%,血红密孔菌对不同木材的腐朽能力差异较大,可造成41.37%的橡木质量损失率,而对柚木Tectona grandis仅有2.20%。通过分析酶活性和木材腐朽结果可以得出,真菌木质纤维素酶活力和其木材腐朽能力不存在相关性,即较高的酶活力并不代表其具有较强的木材腐朽能力。展开更多
文摘Four wood rot fungi were used to decay 300 mature trees of white birch in Liangshui experiment stations,and the mass loss of the samples was measured to study bio-degradability of the wood by the fungi. The decaying susceptible and resistant populations in 10 trees each with the highest and lowest mass loss were selected,in which some main chemical components in decayed and non-decayed wood such as lignin and cellulose and so on was tested. The result showed that the decomposing power of Coriolus versicolor to wood samples was better,and then were Fomes fomentarius,Irpex lacteus and Pholiota adiposa were poor. 1%NaOH extraction in decayed wood was significant higher than that in non-decayed control. Benzene-alcohol extraction in the wood decayed by Coriolus versicolor and Fomes fomentarius was significant higher than that in non-decayed control,but much lower than the control in the wood decayed by Irpex lacteus. The lignin decomposing rate by Irpex lacteus,Pholiota adipose and Fomes fomentarius were faster than the cellulose decomposing rate,the contrary result was achieved by Coriolus versicolor. This paper would provide some useful information in future study in the course and mechanism of wood decaying by rot fungi,and select engineering fungi for paper-making industry and pollution control.
文摘Five wood rot fungi were used to decay 300 mature trees of white birch(Betula platyphylla)in Liangshui experiment stations.The decaying susceptible and resistant populations in 10 trees each with the highest and lowest weight loss were selected,in which total phenol in wood was tested.The result showed that the birch wood with lower total phenol was easy to be decayed by Fomes fomentarius and Piptoporus betulinus;Irpex lacteus,Pholiota adiposa and Coriolus versicolor were not sensitive to total phenol in white birch wood.After GC-MS test to methanol extractionof white birch wood,6 organic compounds were found.This paper would provide some useful information in future study difference in the chemical characteristics of the wood between decaying susceptible and resistant populations,and select natural anti-decadent plants.
