Eighteen species of lady beetles, collected from Pinus armandii infested with Pineus sp. (Homoptera: Adelgidae), in Lijiang, Yunnan province, were enumerated, l of which was described as new to science: Scym...Eighteen species of lady beetles, collected from Pinus armandii infested with Pineus sp. (Homoptera: Adelgidae), in Lijiang, Yunnan province, were enumerated, l of which was described as new to science: Scymnus (Pullus) huashansong, and l as new record for China: Adalia conglomerata (L.). Among these 18 species, 14 were also collected from the hemlocks (Tsuga dumosa, T. forrestii, and T. chinensis ) infested with the hemlock woolly adelgid ( Adelges tsugae ).展开更多
Luseweilei is an easily-bursted microcapsule insecticide. A test of effectiveness of the insecticide to control the larvae of Dendrolimus superans T. was carried out in larch forest in Baoan Forest Farm of Nehe City, ...Luseweilei is an easily-bursted microcapsule insecticide. A test of effectiveness of the insecticide to control the larvae of Dendrolimus superans T. was carried out in larch forest in Baoan Forest Farm of Nehe City, Heilongjiang Province, in April 2001. The solutions of different concentrations (1:150, 1:250, 1:350, and 1:450 Luseweilei : water) were sprayed on the larch trunk before the overwintering larvae climbing on trees and the spraying lengths (height) designed were 1.0, 2.5, and 3.5 m high from ground. The control result showed that spraying 150-, 250-, and 350-fold solutions of the insecticide all produced a good control result, with a mortality rate of 97%, but the 450-fold solution only produced 70% mortality. It is concluded that this insecticide can be used as a kind of good insecticide to control the overwintering larvae of D. superans in spring. Spraying 350-fold solution of easy-burst microcapsule insecticide and one meter spraying length are recommended for the future application..展开更多
[Objective] This study was aimed to review the controlling experience of pine wilt disease in the past 25 years, explore the theories and methods of controlling pine wilt disease, and improve the scientific level of c...[Objective] This study was aimed to review the controlling experience of pine wilt disease in the past 25 years, explore the theories and methods of controlling pine wilt disease, and improve the scientific level of controlling techniques and the protection capacity of healthy pine trees. [Method] Eleven items of effects were used to refine the theory of clearing dead pine trees affected by pine wilt disease, namely, "1 priority", "2 objections", "3 principles", "4 measures", and "5 manage- ments". On the basis of comprehensive control and complete removal of the infect- ed pine trees, a variety of comprehensive and efficient controlling methods were developed to carry out targeted chemical ecology trapping, bionic pesticide killing and releasing natural enemies of Sclerodermus guani, Dastarcus helophoroides. High ef- ficient emamectin benzoate immune injection was developed to inject the healthy pine trees for prevention, so as to extinguish the pine wilt disease. [Result] The pine wilt disease dropped from the peak of 3.5 million dead trees with an infecting area of 28 273 hectares in 1999 to 0.068 million with an area of 4 333 hectares in 2012 gradually, reducing by 98.06% in number and 84.84% in area, respectively. On the basis of removal, Dastarcus helophoroides was also released, which could make the number of dead pines decrease more significantly than the control, and af- ter releasing for 5 consecutive years, the dead pine trees dropped to 0.511 plant/hm2 in 2012, with a mortality rate of 0.022 7%, which achieved the control effect, reaching extremely significant level. "Forest land removal+infected trees isolation+natural enemy release" could extinguish the pine wilt disease. The test of isolating 24 heaps of infected pine trees showed that there were 9 heaps of pine trees extinguished the pine wilt disease, which controlled the occurrence of pine wilt disease for 100%, accounting for 37.5% of the total, in which the number of those isolated using iron netting and nylon net were 4 for each, accounting for 88.9%, and there was one heap using polypropylene net, accounting for 11.1%. The invention of em- amectin benzoate immune injection laid the foundation for extinguishing pine wilt disease. The follow checking of the effects of emamectin benzoate immune injection on pine wilt disease found that the number of dead trees caused by pine wilt dis- ease decreased significantly after injecting, and became very small in October of the next year, and the disease was completely extinguished in the third year. [Conclusionl Pine wilt disease could be controlled and extinguished with positive control by using "comprehensive cleaning+industrialized removal", "comprehensive cleaning+ natural enemy release", "comprehensive cleaning+infected trees isolation+natural ene- my release" and "comprehensive cleaning+emamectin benzoate immune".展开更多
Larch caterpillar (Dendrolimus superans) is very common in the Da Hinggan Mountains, Northeast China, affecting fire regime and forest ecosystem change at large spatio-temporal scales. In this study, we used a spatial...Larch caterpillar (Dendrolimus superans) is very common in the Da Hinggan Mountains, Northeast China, affecting fire regime and forest ecosystem change at large spatio-temporal scales. In this study, we used a spatially explicit landscape model, LANDIS, to simulate the changes of fire regime and forest landscape under four larch caterpillar disturbance intensity levels scenarios in Huzhong forest area, northern of Da Hinggan Mountains. The results indicate that larch caterpillar disturbances would decrease fine fuel load and increase coarse fuel load in the 300 simulation years. Larch caterpillar disturbances would decrease fire frequency in the first 200 years, and the disturbances also decrease fire intensity and fire risk in the early and late stage of simulation. Larch caterpillar disturbances would decrease the area percent of larch cohorts and increase the proportion of white birch, and increase the degree of aggregation of white birch as a result of its strong seed dispersal and colonization ability. Disturbances would also decrease the mature and over-mature larch cohorts and increase all cohorts of white birch, especially the mature and over-mature cohorts. Larch caterpillar disturbances will decrease the stability of forest landscape, therefore,some measures preventing in- sect outbreak and ensuring the sustainable management of forest ecosystem should been taken in the study area.展开更多
The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment, preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and...The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment, preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and agglomerating and the diameter of capsule is in range of 10-30 靘, conformed to the technical requirement. The aftereffect of the 3.3% Beta-Cypermethrin aqueous capsule suspension which was manufactured according to the optimal formula was up more than 25 days. The experiments on controlling the larvae of Dendrolinmus superans Butler and the adults of Xylotrechus rusticus L were carried out with different concentrations of this chemical. The death rate reached 80% when 250 times solution of the chemical was sprayed on stem to control the larvae of D. superans. For control of the adults of X. rusticus, 200, 400 and 600 times solution of the chemical were applied and their control effects (death rate) reached 85.23%, 74.21% and 66.59% respec-tively. Two kinds of solution (200 times and 300 times) of the chemical were used to control the larvae of D. superans in large area, and the control effect for both concentrations was over 90%.展开更多
Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of IT...Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit (LSU) rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.展开更多
A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to...A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil.展开更多
Pasteuria penetrans controls root knots nematodes (Meloidogyne spp.) either by preventing invasion or by causing female sterility. The greatest control effect ofP. penetrans occurred when an efficient quantity ofP. ...Pasteuria penetrans controls root knots nematodes (Meloidogyne spp.) either by preventing invasion or by causing female sterility. The greatest control effect ofP. penetrans occurred when an efficient quantity ofP. penetrans spores attached to nematodes cuticle. The number of spores attaching to J2s within a given time increased with increasing the time of attachment. Based to that, we produced attachment data in vitro recorded encumbered nematodes 1, 3, 6 and 9 h after placing nematodes in a standard P. penetrans spore suspensions. From the count data obtained we modeled P. penetrans attachment using the Poisson and the negative binomial distribution. Attachment count data observed to be over dispersed with respect to high numbers of spores sticks on each J2 after at 6 and 9 h after spores application. We concluded that negative binomial distribution was shown to be the most appropriate model to fit the observed data sets considering that P. penetrans spores are clumped.展开更多
The growth of Pinus tabulaeformis is influenced by the sonsau cocuus sinensis chen. When the insect density is more than 300-350 head/hundred beam conifer, sonsau cocuus sinensis chen occurred significantly influence ...The growth of Pinus tabulaeformis is influenced by the sonsau cocuus sinensis chen. When the insect density is more than 300-350 head/hundred beam conifer, sonsau cocuus sinensis chen occurred significantly influence the Pinus tabulaeformis' s growth. when the insect density reached 250-300 head/100 beam coniferous, sonsau cocuus sinensis chen significantly influence the accumulation of Pinus tabulaeformis volume. To determine the sonsau cocuus sinensis chen 2nd instar nymph theory control index was 220.5 / hundred beam needles has a guiding significance to the production practice.展开更多
Sumithion is widely used to control brittle in paddy fields and tiger bug in fish larval rearing ponds. The objective of this study was to elucidate the toxic effects of sumithion on larval stages of stinging catfish ...Sumithion is widely used to control brittle in paddy fields and tiger bug in fish larval rearing ponds. The objective of this study was to elucidate the toxic effects of sumithion on larval stages of stinging catfish Heteropneustes fossilis. Larvae were exposed to two concentrations(150 and 250 μg/L) of sumithion with one control in three replicates of each. Larvae samples were collected at 20- and 24-h intervals followed by observation under a digital microscope. Exposures of stinging catfish larvae to sumithion produced deformities including irregular head shape, lordosis, yolk sac edema, body arcuation, tissue ulceration, etc. The mortality rates of larvae were significantly increased in response to increase in sumithion concentrations. Furthermore, around 30% of the total adult stinging catfish reared in sumithiontreated aquaculture ponds were found to be deformed permanently. These findings highlight that exposure of stinging catfish to sumithion at the critical and sensitive stages in their life cycle may significantly reduce the number of returning adults. Therefore, the use of sumithion for crop protection needs to be considered carefully and alternatives to sumithion should to be developed for controlling aquatic insects in aqua-ponds during larval rearing.展开更多
The rDNA ITS2 regions of T. dendrolimi Matsumura and T. ostriniae Pang et Chen (Hymenoptera: Trichogrammatidae) were cloned and sequenced. The homologous sequences available in GenBank were retrieved and...The rDNA ITS2 regions of T. dendrolimi Matsumura and T. ostriniae Pang et Chen (Hymenoptera: Trichogrammatidae) were cloned and sequenced. The homologous sequences available in GenBank were retrieved and analyzed, and then specific primers were designed for molecular identification and detection of T. dendrolimi. Repeated screening showed that PCR amplification by the diagnostic primers enabled the differentiation of not only bulk samples and single adult (male or female), but also eggs and juveniles, which was not possible by conventional methods. The advantage of this system over morphology based systems is that non specialists are able to identify individuals or trace specimens efficiently. The derived molecular detection technique was then used to identify 12 specimens collected from different localities on the Chinese mainland; the results showed that this protocol could be applied to molecular monitoring of Trichogramma species in the field.Finally,ITS2s of 6 geographical populations of T.dendrolimi (TdCHA,TDJL,TdXZ,TdRH,TdGZ and TdYBL) were cloned and sequenced.The multialignment analysis of intraspecific ITS2 se quences showed that the diagnostic primers have their own theoretical bases.展开更多
A 2-year-old pine plantation was selected to receive treatments of fertilizers and herbicides to evaluate effects on Nantucket pine tip moth infestations and the tree growth parameters of height, diameter and volume i...A 2-year-old pine plantation was selected to receive treatments of fertilizers and herbicides to evaluate effects on Nantucket pine tip moth infestations and the tree growth parameters of height, diameter and volume increment. Nitrogen and phosphorus fertilizers, and hexazinone and sulfometuron methyl herbicides were used in creating six treatments: (i) control; (ii) phosphorus; (iii) nitrogen and phosphorus; (iv) phosphorus and herbicide; (v) nitrogen, phosphorus and herbicide; and (vi) herbicide. Treatments were applied in 1987 and 1988. In 1987, trees treated with nitrogen, phosphorus and herbicide had significantly greater height, diameter and volume growth than trees not receiving fertilizer treatments, but did not have significantly higher tip moth infestations than control trees. Treatments receiving phosphorus only had much lower tip moth infestation rates than other treatments except nitrogen and phosphorus. In 1988, tip moth infestations were uniformly low, with no differences in treatment effects observed.展开更多
The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, ...The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, etc. By virtue of so much complicated interplay among the biological factors related to pine wilt disease which is different from other forest disease, it also points out that the further researches about pine wilt disease should focus on interplay mechanism of key biological factors to discover pathogenic mechanism, and simple and quick inspection and quarantine methods.展开更多
The change of pine scale (Hemiberlesia pitysophila Takagi) population density on pine forest in urban park between treatment (tending operations) and CK (no tending operations) was researched in this paper. The result...The change of pine scale (Hemiberlesia pitysophila Takagi) population density on pine forest in urban park between treatment (tending operations) and CK (no tending operations) was researched in this paper. The results were shown as follows: population density declined to 0.63 head per bundle from 2.61 heads per bundle when crown density of pine in urban park had become 0.5 or so by means of cultivation activities such as felling, pruning and thinning and so on, and drop got to 75.86 percent. The pinewoods would soon get revived once using these cultivation activities, so tending operations was main measures controlling over harm of pine scale in urban park; At the same time, population density of pine scale on pinewoods in the sunny slope was more than that in the somber slope and it had become less and less from sunny slope to somber slope. Therefore, tree species must be chosen in the planning and design of urban park for preventing harm and happening of pine scale.展开更多
This article briefly introduced the disease incidence and disservice situation in China. The disease is regarded as cancer in pines for its higher spread and death speed and the difficulty in prevention. Strict quaran...This article briefly introduced the disease incidence and disservice situation in China. The disease is regarded as cancer in pines for its higher spread and death speed and the difficulty in prevention. Strict quarantine is the main measure on countrol of the disease, for there are many difficulties in the prevention. The most important part of the disease study is the early diagnosis and detection.展开更多
Large areas of forest plantations have been developed in China. It is important to evaluate the soil fauna in plantations and the conditions needed for their recovery in view of the large areas of plantations in China...Large areas of forest plantations have been developed in China. It is important to evaluate the soil fauna in plantations and the conditions needed for their recovery in view of the large areas of plantations in China. Three Pinus tabulaeformis forests, a 26-year-old plantation (P26) and a 45-year-old plantation (P45), exposed to clear-cutting before plantation, and an 80 260-year-old natural forest (N260), were chosen to study the effects of different forest ages/types on Collembola community in the lifter and soil layers during 2008 and 2009. Soil conditions in P26 and P45 were significantly deteriorated when compared to N260. A higher value of soil bulk density and lower values of soil organic matter, soil N, litter depth, soil pH, and soil water content were observed in P26 and P45. Totally, the same genera of Collembola tended to occur in the forests of all ages studied; however, the Collembola community structure was significantly impacted by the differences in forest age. Both in the litter and soil layers, the density and generic richness of the Collembola were the highest in N260 and the lowest in P26. Some collembolan groups were sensitive to soil conditions in particular forest ages. N260 was associated with relatively high abundance of Plutomus collembolans and P45 with relatively high abundance of Pseudofolsomia collembolans. The canonical correspondence analysis showed that the community structure of Collembola was mainly affected by forest age in both litter and soil layer. The ordination analysis of non-metric multidimensional scaling also found that the Collembola community did not recover to the level of natural forests in 26-year regeneration after clear-cutting. Even in 45-year regeneration after clear-cutting, the Collembola community only showed a slight recovery to the level of natural forests. Our results clearly showed that both Collembola community and soil conditions did not recover in 26- and 45-year regeneration after clear-cutting in P. tabulaeforrnis plantations; however, they might have the potential to recover in the future because the same genera of Collembola were distributed in the plantations and natural forests.展开更多
Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. al...Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle's immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.展开更多
文摘Eighteen species of lady beetles, collected from Pinus armandii infested with Pineus sp. (Homoptera: Adelgidae), in Lijiang, Yunnan province, were enumerated, l of which was described as new to science: Scymnus (Pullus) huashansong, and l as new record for China: Adalia conglomerata (L.). Among these 18 species, 14 were also collected from the hemlocks (Tsuga dumosa, T. forrestii, and T. chinensis ) infested with the hemlock woolly adelgid ( Adelges tsugae ).
文摘Luseweilei is an easily-bursted microcapsule insecticide. A test of effectiveness of the insecticide to control the larvae of Dendrolimus superans T. was carried out in larch forest in Baoan Forest Farm of Nehe City, Heilongjiang Province, in April 2001. The solutions of different concentrations (1:150, 1:250, 1:350, and 1:450 Luseweilei : water) were sprayed on the larch trunk before the overwintering larvae climbing on trees and the spraying lengths (height) designed were 1.0, 2.5, and 3.5 m high from ground. The control result showed that spraying 150-, 250-, and 350-fold solutions of the insecticide all produced a good control result, with a mortality rate of 97%, but the 450-fold solution only produced 70% mortality. It is concluded that this insecticide can be used as a kind of good insecticide to control the overwintering larvae of D. superans in spring. Spraying 350-fold solution of easy-burst microcapsule insecticide and one meter spraying length are recommended for the future application..
基金Supported by the Scientific Research Project of National Level of YANG Zhongqi of Chinese Academy of Forestry(2012AA101503)~~
文摘[Objective] This study was aimed to review the controlling experience of pine wilt disease in the past 25 years, explore the theories and methods of controlling pine wilt disease, and improve the scientific level of controlling techniques and the protection capacity of healthy pine trees. [Method] Eleven items of effects were used to refine the theory of clearing dead pine trees affected by pine wilt disease, namely, "1 priority", "2 objections", "3 principles", "4 measures", and "5 manage- ments". On the basis of comprehensive control and complete removal of the infect- ed pine trees, a variety of comprehensive and efficient controlling methods were developed to carry out targeted chemical ecology trapping, bionic pesticide killing and releasing natural enemies of Sclerodermus guani, Dastarcus helophoroides. High ef- ficient emamectin benzoate immune injection was developed to inject the healthy pine trees for prevention, so as to extinguish the pine wilt disease. [Result] The pine wilt disease dropped from the peak of 3.5 million dead trees with an infecting area of 28 273 hectares in 1999 to 0.068 million with an area of 4 333 hectares in 2012 gradually, reducing by 98.06% in number and 84.84% in area, respectively. On the basis of removal, Dastarcus helophoroides was also released, which could make the number of dead pines decrease more significantly than the control, and af- ter releasing for 5 consecutive years, the dead pine trees dropped to 0.511 plant/hm2 in 2012, with a mortality rate of 0.022 7%, which achieved the control effect, reaching extremely significant level. "Forest land removal+infected trees isolation+natural enemy release" could extinguish the pine wilt disease. The test of isolating 24 heaps of infected pine trees showed that there were 9 heaps of pine trees extinguished the pine wilt disease, which controlled the occurrence of pine wilt disease for 100%, accounting for 37.5% of the total, in which the number of those isolated using iron netting and nylon net were 4 for each, accounting for 88.9%, and there was one heap using polypropylene net, accounting for 11.1%. The invention of em- amectin benzoate immune injection laid the foundation for extinguishing pine wilt disease. The follow checking of the effects of emamectin benzoate immune injection on pine wilt disease found that the number of dead trees caused by pine wilt dis- ease decreased significantly after injecting, and became very small in October of the next year, and the disease was completely extinguished in the third year. [Conclusionl Pine wilt disease could be controlled and extinguished with positive control by using "comprehensive cleaning+industrialized removal", "comprehensive cleaning+ natural enemy release", "comprehensive cleaning+infected trees isolation+natural ene- my release" and "comprehensive cleaning+emamectin benzoate immune".
基金Under the auspices of National Natural Science Foundation of China(No.31070422,40871245)Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA05050201)
文摘Larch caterpillar (Dendrolimus superans) is very common in the Da Hinggan Mountains, Northeast China, affecting fire regime and forest ecosystem change at large spatio-temporal scales. In this study, we used a spatially explicit landscape model, LANDIS, to simulate the changes of fire regime and forest landscape under four larch caterpillar disturbance intensity levels scenarios in Huzhong forest area, northern of Da Hinggan Mountains. The results indicate that larch caterpillar disturbances would decrease fine fuel load and increase coarse fuel load in the 300 simulation years. Larch caterpillar disturbances would decrease fire frequency in the first 200 years, and the disturbances also decrease fire intensity and fire risk in the early and late stage of simulation. Larch caterpillar disturbances would decrease the area percent of larch cohorts and increase the proportion of white birch, and increase the degree of aggregation of white birch as a result of its strong seed dispersal and colonization ability. Disturbances would also decrease the mature and over-mature larch cohorts and increase all cohorts of white birch, especially the mature and over-mature cohorts. Larch caterpillar disturbances will decrease the stability of forest landscape, therefore,some measures preventing in- sect outbreak and ensuring the sustainable management of forest ecosystem should been taken in the study area.
文摘The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment, preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and agglomerating and the diameter of capsule is in range of 10-30 靘, conformed to the technical requirement. The aftereffect of the 3.3% Beta-Cypermethrin aqueous capsule suspension which was manufactured according to the optimal formula was up more than 25 days. The experiments on controlling the larvae of Dendrolinmus superans Butler and the adults of Xylotrechus rusticus L were carried out with different concentrations of this chemical. The death rate reached 80% when 250 times solution of the chemical was sprayed on stem to control the larvae of D. superans. For control of the adults of X. rusticus, 200, 400 and 600 times solution of the chemical were applied and their control effects (death rate) reached 85.23%, 74.21% and 66.59% respec-tively. Two kinds of solution (200 times and 300 times) of the chemical were used to control the larvae of D. superans in large area, and the control effect for both concentrations was over 90%.
基金Project (No.2002C34003) supported by the Science and Technology Department of Zhejiang Province,China
文摘Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit (LSU) rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.
文摘A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil.
文摘Pasteuria penetrans controls root knots nematodes (Meloidogyne spp.) either by preventing invasion or by causing female sterility. The greatest control effect ofP. penetrans occurred when an efficient quantity ofP. penetrans spores attached to nematodes cuticle. The number of spores attaching to J2s within a given time increased with increasing the time of attachment. Based to that, we produced attachment data in vitro recorded encumbered nematodes 1, 3, 6 and 9 h after placing nematodes in a standard P. penetrans spore suspensions. From the count data obtained we modeled P. penetrans attachment using the Poisson and the negative binomial distribution. Attachment count data observed to be over dispersed with respect to high numbers of spores sticks on each J2 after at 6 and 9 h after spores application. We concluded that negative binomial distribution was shown to be the most appropriate model to fit the observed data sets considering that P. penetrans spores are clumped.
文摘The growth of Pinus tabulaeformis is influenced by the sonsau cocuus sinensis chen. When the insect density is more than 300-350 head/hundred beam conifer, sonsau cocuus sinensis chen occurred significantly influence the Pinus tabulaeformis' s growth. when the insect density reached 250-300 head/100 beam coniferous, sonsau cocuus sinensis chen significantly influence the accumulation of Pinus tabulaeformis volume. To determine the sonsau cocuus sinensis chen 2nd instar nymph theory control index was 220.5 / hundred beam needles has a guiding significance to the production practice.
文摘Sumithion is widely used to control brittle in paddy fields and tiger bug in fish larval rearing ponds. The objective of this study was to elucidate the toxic effects of sumithion on larval stages of stinging catfish Heteropneustes fossilis. Larvae were exposed to two concentrations(150 and 250 μg/L) of sumithion with one control in three replicates of each. Larvae samples were collected at 20- and 24-h intervals followed by observation under a digital microscope. Exposures of stinging catfish larvae to sumithion produced deformities including irregular head shape, lordosis, yolk sac edema, body arcuation, tissue ulceration, etc. The mortality rates of larvae were significantly increased in response to increase in sumithion concentrations. Furthermore, around 30% of the total adult stinging catfish reared in sumithiontreated aquaculture ponds were found to be deformed permanently. These findings highlight that exposure of stinging catfish to sumithion at the critical and sensitive stages in their life cycle may significantly reduce the number of returning adults. Therefore, the use of sumithion for crop protection needs to be considered carefully and alternatives to sumithion should to be developed for controlling aquatic insects in aqua-ponds during larval rearing.
文摘The rDNA ITS2 regions of T. dendrolimi Matsumura and T. ostriniae Pang et Chen (Hymenoptera: Trichogrammatidae) were cloned and sequenced. The homologous sequences available in GenBank were retrieved and analyzed, and then specific primers were designed for molecular identification and detection of T. dendrolimi. Repeated screening showed that PCR amplification by the diagnostic primers enabled the differentiation of not only bulk samples and single adult (male or female), but also eggs and juveniles, which was not possible by conventional methods. The advantage of this system over morphology based systems is that non specialists are able to identify individuals or trace specimens efficiently. The derived molecular detection technique was then used to identify 12 specimens collected from different localities on the Chinese mainland; the results showed that this protocol could be applied to molecular monitoring of Trichogramma species in the field.Finally,ITS2s of 6 geographical populations of T.dendrolimi (TdCHA,TDJL,TdXZ,TdRH,TdGZ and TdYBL) were cloned and sequenced.The multialignment analysis of intraspecific ITS2 se quences showed that the diagnostic primers have their own theoretical bases.
文摘A 2-year-old pine plantation was selected to receive treatments of fertilizers and herbicides to evaluate effects on Nantucket pine tip moth infestations and the tree growth parameters of height, diameter and volume increment. Nitrogen and phosphorus fertilizers, and hexazinone and sulfometuron methyl herbicides were used in creating six treatments: (i) control; (ii) phosphorus; (iii) nitrogen and phosphorus; (iv) phosphorus and herbicide; (v) nitrogen, phosphorus and herbicide; and (vi) herbicide. Treatments were applied in 1987 and 1988. In 1987, trees treated with nitrogen, phosphorus and herbicide had significantly greater height, diameter and volume growth than trees not receiving fertilizer treatments, but did not have significantly higher tip moth infestations than control trees. Treatments receiving phosphorus only had much lower tip moth infestation rates than other treatments except nitrogen and phosphorus. In 1988, tip moth infestations were uniformly low, with no differences in treatment effects observed.
基金The paper is supported by National Basic Research and Development Program, 2002CB111404.
文摘The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, etc. By virtue of so much complicated interplay among the biological factors related to pine wilt disease which is different from other forest disease, it also points out that the further researches about pine wilt disease should focus on interplay mechanism of key biological factors to discover pathogenic mechanism, and simple and quick inspection and quarantine methods.
文摘The change of pine scale (Hemiberlesia pitysophila Takagi) population density on pine forest in urban park between treatment (tending operations) and CK (no tending operations) was researched in this paper. The results were shown as follows: population density declined to 0.63 head per bundle from 2.61 heads per bundle when crown density of pine in urban park had become 0.5 or so by means of cultivation activities such as felling, pruning and thinning and so on, and drop got to 75.86 percent. The pinewoods would soon get revived once using these cultivation activities, so tending operations was main measures controlling over harm of pine scale in urban park; At the same time, population density of pine scale on pinewoods in the sunny slope was more than that in the somber slope and it had become less and less from sunny slope to somber slope. Therefore, tree species must be chosen in the planning and design of urban park for preventing harm and happening of pine scale.
文摘This article briefly introduced the disease incidence and disservice situation in China. The disease is regarded as cancer in pines for its higher spread and death speed and the difficulty in prevention. Strict quarantine is the main measure on countrol of the disease, for there are many difficulties in the prevention. The most important part of the disease study is the early diagnosis and detection.
基金supported by the Innovation Pro-gram of Chinese Academy of Science(No.KSCX2EW-Z-6)the Special Program of the Ministry of Science and Technology,China(Nos.2009ZX08012-005B and 2012ZX08011002)the National Natural Sciences Foundation of China(No.31200331)
文摘Large areas of forest plantations have been developed in China. It is important to evaluate the soil fauna in plantations and the conditions needed for their recovery in view of the large areas of plantations in China. Three Pinus tabulaeformis forests, a 26-year-old plantation (P26) and a 45-year-old plantation (P45), exposed to clear-cutting before plantation, and an 80 260-year-old natural forest (N260), were chosen to study the effects of different forest ages/types on Collembola community in the lifter and soil layers during 2008 and 2009. Soil conditions in P26 and P45 were significantly deteriorated when compared to N260. A higher value of soil bulk density and lower values of soil organic matter, soil N, litter depth, soil pH, and soil water content were observed in P26 and P45. Totally, the same genera of Collembola tended to occur in the forests of all ages studied; however, the Collembola community structure was significantly impacted by the differences in forest age. Both in the litter and soil layers, the density and generic richness of the Collembola were the highest in N260 and the lowest in P26. Some collembolan groups were sensitive to soil conditions in particular forest ages. N260 was associated with relatively high abundance of Plutomus collembolans and P45 with relatively high abundance of Pseudofolsomia collembolans. The canonical correspondence analysis showed that the community structure of Collembola was mainly affected by forest age in both litter and soil layer. The ordination analysis of non-metric multidimensional scaling also found that the Collembola community did not recover to the level of natural forests in 26-year regeneration after clear-cutting. Even in 45-year regeneration after clear-cutting, the Collembola community only showed a slight recovery to the level of natural forests. Our results clearly showed that both Collembola community and soil conditions did not recover in 26- and 45-year regeneration after clear-cutting in P. tabulaeforrnis plantations; however, they might have the potential to recover in the future because the same genera of Collembola were distributed in the plantations and natural forests.
基金supported by the Forestry Industry Research Special Funds for Public Welfare Project(201204501)National Key Plan for Scientific Research and Development of China(2016YFC1200604,2016YFD0500300)+2 种基金the High Technology Research and Development Program(HTRDP)of China(2014AA020529)National Natural Science Foundation of China(31572272,31370650,31402013,31221091,31672291,L1524009)the CAS Key Research Projects of the Frontier Science(QYZDBSSW-SMC014),and CAS(2015-SM-C-02)
文摘Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle's immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.