[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Re...[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.展开更多
PSⅡ core antenna complexes, CP43 and CP47, were purified from spinach (Spinacia oleracea L.) by DEAE Fractogel TSK 650S anion exchange chromatography. Their normal temperature (298 K) resonance Raman spectra were...PSⅡ core antenna complexes, CP43 and CP47, were purified from spinach (Spinacia oleracea L.) by DEAE Fractogel TSK 650S anion exchange chromatography. Their normal temperature (298 K) resonance Raman spectra were measured. The results suggest that all β carotenoids bound to CP43 and CP47 are in all trans configuration and likely in twisted conformations.展开更多
The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of...The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of farmers and agricultural benefits in the tropical area of China. This study summarized the major advances on cashew germplasm resources in China, analyzed the major research progress and existing problems from the aspects of the collection and evaluation, resistances, genetic diversity of cashew germplasm resources, and put forward the work ideas to analyze the construction of core germplasm bank and the genetic stability of the major biological characteristics of cashew germplasm resources, which were of important significance for the improvement of the cultivar classification and resource evaluation system of cashew germplasm resources in China.展开更多
In that DNA diversity detected nowadays could not mean phenotypic diversity,it is the precondition of breeding project and basic research of crop improvement that genetic diversity analysis and sampling of core collec...In that DNA diversity detected nowadays could not mean phenotypic diversity,it is the precondition of breeding project and basic research of crop improvement that genetic diversity analysis and sampling of core collection by phenotypes.Phenotyping and statistic analysis on 9 traits of 92 accessions of cotton germplasm resource from three species（Gossypium hirsutum L.,Gossypium barbadence L.and Gossypium arboreum L.）were conducted.And the statistics（variation coefficient,proportion of special accessions and Shannon-Weaver information index）indicated that initial collection had abundant phenotypic diversity;software NTSYS-pc and the unweighted pair group method of arithmetic（UPGMA）were used for the cluster analysis on genetic similarity coefficient and genetic distance matrix,and the result showed that the genetic relationship among accessions was highly consistent with the pedigree;22 accessions of core collection were selected by software QGAStation,four statistics,such as variance difference percentage（VD%）,mean difference percentage（MD%）,coincidence rate（CR%）and variable rate（VR%）,showed that the genetic diversity of core collection was approximately equal to the initial collection.The results of genetic diversity analysis based on phenotypic data and sampling of core collection would provide reference for breeding projects and basic research.展开更多
Using the Java 2 enterprise edition (J2EE), the industry standard technique, a component and web-based manufacturing execution system (MES), is presented. It is considered to be open, modularized, distributed, configu...Using the Java 2 enterprise edition (J2EE), the industry standard technique, a component and web-based manufacturing execution system (MES), is presented. It is considered to be open, modularized, distributed, configurable, interoperable and maintainable. After introducing some core techniques of J2EE as the basic foundations of the proposed MES, this paper briefly analyzes the MES domain in manufacturing systems and constructs the abstract object and component model, and then establishes the MES architecture. This MES can be facilely integrated with other manufacturing applications. Finally, an example of integration with the enterprise resource planning (ERP) system is given.展开更多
To solve the contradiction between supply and demand of land, the con- struction of land resource security guarantee system is an important part to coordi- nate the economic development and cultivated land protection ...To solve the contradiction between supply and demand of land, the con- struction of land resource security guarantee system is an important part to coordi- nate the economic development and cultivated land protection in the core grain pro- duction region. Based on the deep analysis on the land resources characteristics and problems using the combined method of literature review method with qualita- tive and quantitative analysis, we constructed the land resource security system for different categories according to the land use principles of optimization, intensivism, improvement, and overall planning, and proposed the corresponding supporting mea- sures. The results showed that controlling the new increase, promoting the reclama- tion and improvement of land are the basis for land resource security, clearing the idle, improving the utilization rate of stock are the core of the efficient use of land resources, and establishing a unified urban and rural construction land market is the key to promoting the economical and intensive utilization of land resources, provid- ing scientific basis for the sustainable utilization of land resources in the core grain production regions.展开更多
By using diamond model, the current development status and core competitiveness of the rice seed industry in Guangxi Province were analyzed in detail from the 6 aspects of production factors, demand factors, related i...By using diamond model, the current development status and core competitiveness of the rice seed industry in Guangxi Province were analyzed in detail from the 6 aspects of production factors, demand factors, related industries and support industries, firm strategy, chance factor and government factor, which clarified the advantages of the rice seed industry of Guangxi in the nature and technological factors, firm strategy and government policy, and the disadvantages in demand factors, production cost, infrastructure and related industries. And the corresponding countermeasures were proposed to enhance the competitiveness of the rice seed industry of Guangxi.展开更多
The aim of this work was to improve the rate of conventionally blind enucleation for bovine somatic cell nuclear transfer. The cross section of a 0.5 ml Eppendorf tube was attached with a sheet of 400 mesh/inch2-cell ...The aim of this work was to improve the rate of conventionally blind enucleation for bovine somatic cell nuclear transfer. The cross section of a 0.5 ml Eppendorf tube was attached with a sheet of 400 mesh/inch2-cell screen after the bottom of the Eppendorf tube had been cut, and put into a 1 mL Eppendorf tube. In experiment 1, the oocytes in the metaphase Ⅱ stage were placed on the membrane in the Eppendorf tube, and centrifuged at 1,000, 2,000, or 3,000 r/min for 10 min, respectively. The oocytes were stained with Hoechst 33342 and then the relative position of the first polar body to the chromosomes, and the efficiency of enucleation were evaluated. In experiment 2, enucleated oocytes were fused with granulosa cells, following centrifugation and enucleation, and the potential development of the reconstituted embryos was estimated. The results indicated that the rate of enucleation in oocytes after centrifugation at 2,000 r/min for 10 min was 86.6% with an angle less then 20° between the first polar body and chromosomes. The rate of enucleation in cells spun at 2,000 r/min was higher than that of controls （87.4% vs. 64.4%, P 〈 0.05）. Fur- thermore; centrifugation of recipient oocytes did not have a detrimental effect on the development of reconstituted embryos following nuclear transfer. In conclusion, centrifugation assisted enucleation may significantly improve the rate of bovine oocyte enucleation.展开更多
Ultrafast time_resolved fluorescence experiments have been performed with core antennas CP43 and CP47 of PS Ⅱ. Their dynamic fluorescence spectra were obtained with excitation wavelength 514.5 nm. For CP43, the emiss...Ultrafast time_resolved fluorescence experiments have been performed with core antennas CP43 and CP47 of PS Ⅱ. Their dynamic fluorescence spectra were obtained with excitation wavelength 514.5 nm. For CP43, the emission spectrum was found to be from 640 to 780 nm with a peak at ～680 nm and the lifetime of fluorescence was 3.54 ns. For CP47, the emission spectrum was from 630 to 775 nm with a peak at ～691 nm and the fluorescence lifetime was 3.22 ns. The fluorescence emission efficiencies of Chl a in CP43 and CP47 were calculated to be approximately 38.3% and 40.6%, respectively. The energy transfer from β_Car to Chl a in CP43 and CP47 was discussed. The rates of energy transfer from β_Car to Chl a were measured to be about 9.6×10 11 s -1 and 1.3×10 12 s -1 and energy transfer efficiencies 47.5% and 66.5% respectively. The edge_edge distances between β_Car and Chl a in CP43 and CP47 were estimated to be ～0.110 nm and ～0.085 nm respectively.展开更多
基金Supported by Excellent Team Training Program of Yunnan Academy of Agriculture Sciences(YAAS2014YY002)~~
文摘[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.
文摘PSⅡ core antenna complexes, CP43 and CP47, were purified from spinach (Spinacia oleracea L.) by DEAE Fractogel TSK 650S anion exchange chromatography. Their normal temperature (298 K) resonance Raman spectra were measured. The results suggest that all β carotenoids bound to CP43 and CP47 are in all trans configuration and likely in twisted conformations.
基金Supported by the National Nonprofit Institute Research Grant of CATAS-TCGRI(16300320150331630032014032+1 种基金PZSFYL-201613)the Ministry of Agriculture Tropical Crop Germplasm Resources Protection(16RZZY-101)~~
文摘The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of farmers and agricultural benefits in the tropical area of China. This study summarized the major advances on cashew germplasm resources in China, analyzed the major research progress and existing problems from the aspects of the collection and evaluation, resistances, genetic diversity of cashew germplasm resources, and put forward the work ideas to analyze the construction of core germplasm bank and the genetic stability of the major biological characteristics of cashew germplasm resources, which were of important significance for the improvement of the cultivar classification and resource evaluation system of cashew germplasm resources in China.
基金Supported by National Natural Science Foundation of China(30971821)the Research Fund for the Doctoral Program of Higher Education of China(20090204120017)"Program for Key Academic Youths"of Northwest A&F University(Z111020712)~~
文摘In that DNA diversity detected nowadays could not mean phenotypic diversity,it is the precondition of breeding project and basic research of crop improvement that genetic diversity analysis and sampling of core collection by phenotypes.Phenotyping and statistic analysis on 9 traits of 92 accessions of cotton germplasm resource from three species（Gossypium hirsutum L.,Gossypium barbadence L.and Gossypium arboreum L.）were conducted.And the statistics（variation coefficient,proportion of special accessions and Shannon-Weaver information index）indicated that initial collection had abundant phenotypic diversity;software NTSYS-pc and the unweighted pair group method of arithmetic（UPGMA）were used for the cluster analysis on genetic similarity coefficient and genetic distance matrix,and the result showed that the genetic relationship among accessions was highly consistent with the pedigree;22 accessions of core collection were selected by software QGAStation,four statistics,such as variance difference percentage（VD%）,mean difference percentage（MD%）,coincidence rate（CR%）and variable rate（VR%）,showed that the genetic diversity of core collection was approximately equal to the initial collection.The results of genetic diversity analysis based on phenotypic data and sampling of core collection would provide reference for breeding projects and basic research.
文摘Using the Java 2 enterprise edition (J2EE), the industry standard technique, a component and web-based manufacturing execution system (MES), is presented. It is considered to be open, modularized, distributed, configurable, interoperable and maintainable. After introducing some core techniques of J2EE as the basic foundations of the proposed MES, this paper briefly analyzes the MES domain in manufacturing systems and constructs the abstract object and component model, and then establishes the MES architecture. This MES can be facilely integrated with other manufacturing applications. Finally, an example of integration with the enterprise resource planning (ERP) system is given.
文摘To solve the contradiction between supply and demand of land, the con- struction of land resource security guarantee system is an important part to coordi- nate the economic development and cultivated land protection in the core grain pro- duction region. Based on the deep analysis on the land resources characteristics and problems using the combined method of literature review method with qualita- tive and quantitative analysis, we constructed the land resource security system for different categories according to the land use principles of optimization, intensivism, improvement, and overall planning, and proposed the corresponding supporting mea- sures. The results showed that controlling the new increase, promoting the reclama- tion and improvement of land are the basis for land resource security, clearing the idle, improving the utilization rate of stock are the core of the efficient use of land resources, and establishing a unified urban and rural construction land market is the key to promoting the economical and intensive utilization of land resources, provid- ing scientific basis for the sustainable utilization of land resources in the core grain production regions.
文摘By using diamond model, the current development status and core competitiveness of the rice seed industry in Guangxi Province were analyzed in detail from the 6 aspects of production factors, demand factors, related industries and support industries, firm strategy, chance factor and government factor, which clarified the advantages of the rice seed industry of Guangxi in the nature and technological factors, firm strategy and government policy, and the disadvantages in demand factors, production cost, infrastructure and related industries. And the corresponding countermeasures were proposed to enhance the competitiveness of the rice seed industry of Guangxi.
基金This work was supported by the National High Technology Research and Development Program of China(863 Program)(No.2001 AA213081).
文摘The aim of this work was to improve the rate of conventionally blind enucleation for bovine somatic cell nuclear transfer. The cross section of a 0.5 ml Eppendorf tube was attached with a sheet of 400 mesh/inch2-cell screen after the bottom of the Eppendorf tube had been cut, and put into a 1 mL Eppendorf tube. In experiment 1, the oocytes in the metaphase Ⅱ stage were placed on the membrane in the Eppendorf tube, and centrifuged at 1,000, 2,000, or 3,000 r/min for 10 min, respectively. The oocytes were stained with Hoechst 33342 and then the relative position of the first polar body to the chromosomes, and the efficiency of enucleation were evaluated. In experiment 2, enucleated oocytes were fused with granulosa cells, following centrifugation and enucleation, and the potential development of the reconstituted embryos was estimated. The results indicated that the rate of enucleation in oocytes after centrifugation at 2,000 r/min for 10 min was 86.6% with an angle less then 20° between the first polar body and chromosomes. The rate of enucleation in cells spun at 2,000 r/min was higher than that of controls （87.4% vs. 64.4%, P 〈 0.05）. Fur- thermore; centrifugation of recipient oocytes did not have a detrimental effect on the development of reconstituted embryos following nuclear transfer. In conclusion, centrifugation assisted enucleation may significantly improve the rate of bovine oocyte enucleation.
文摘Ultrafast time_resolved fluorescence experiments have been performed with core antennas CP43 and CP47 of PS Ⅱ. Their dynamic fluorescence spectra were obtained with excitation wavelength 514.5 nm. For CP43, the emission spectrum was found to be from 640 to 780 nm with a peak at ～680 nm and the lifetime of fluorescence was 3.54 ns. For CP47, the emission spectrum was from 630 to 775 nm with a peak at ～691 nm and the fluorescence lifetime was 3.22 ns. The fluorescence emission efficiencies of Chl a in CP43 and CP47 were calculated to be approximately 38.3% and 40.6%, respectively. The energy transfer from β_Car to Chl a in CP43 and CP47 was discussed. The rates of energy transfer from β_Car to Chl a were measured to be about 9.6×10 11 s -1 and 1.3×10 12 s -1 and energy transfer efficiencies 47.5% and 66.5% respectively. The edge_edge distances between β_Car and Chl a in CP43 and CP47 were estimated to be ～0.110 nm and ～0.085 nm respectively.
