[ Objective] The study was to compare the structural and moisture characteristics of leaf from the plantlets of three types of omamental lily（ Lilium brownii）. [ Method ] The paraffin sections of leaves of tested li...[ Objective] The study was to compare the structural and moisture characteristics of leaf from the plantlets of three types of omamental lily（ Lilium brownii）. [ Method ] The paraffin sections of leaves of tested lily varieties were prepared and then observed under microscope, and the stomatal characteristics and moisture characteristics of tested lily varieties were measured. I Resaltl All the three ornamental lily varieties show isobilateral leaf, single layer of epicuticula and lower epidermis, and no obvious differentiation of palisade tissue and spongy tissue; their stomata distribute in lower epidermis, and the guard cells are dumbbell-shaped; all of these matedais present high moisture. For the leaf sick- ness, midrib sickness and mesophyll tissue sickness, the order was determined to be oriental lily 〉 Lilium/ongiflorum 〉 Asian lily; of the three types of ornamental lily, Ulium Iongiflorum has the largest stomatai aperture and Asian lily has the smallest; focusing the water potential and moisture, the turn was Asian lily 〉 oriental lily 〉 Lilium Iong＇fflorum. [ Condusion] The study may facilitate the artificial regulation of the growth conditions of the plantlets of ornamental lily.展开更多
[Objective] The study had developed a means of rapid propagation Pteris vittata L.by tissue culture. The species was a perennial fern belonging to the genus Pteris. [Metbed] The leaf bud of P. vittata collected in fie...[Objective] The study had developed a means of rapid propagation Pteris vittata L.by tissue culture. The species was a perennial fern belonging to the genus Pteris. [Metbed] The leaf bud of P. vittata collected in field conditions as explantsand the 1/2 MS ＋ 3% sucrose ＋ 0.7% agar as the basic medium were used to screen the medium formula of the phytohormone ratio for callus induction and subculture of P. vittata. [Result] The best medium formula for each step was list below： 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.1 mg/L KT ＋ 0.5 mg/L 2, 4-D for in- ducing the callus from explants; 1/2MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 1.0 mg/L KT ＋ 0.01 mg/L 2,4-D for inducing the GGB from callus and the seedlings from GGB. In addition, 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.5 mg/L 2,4-D for the subculture could make the continued proliferation of callus. [Cen- clusioa] This study makes an applicable procedure by the direct use of field materi- als, for propagating P. vittata in a simplified and rapid mode.展开更多
This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has bee...This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.展开更多
[Objective] This study was to develop an in vitro tissue culture system of Rosa spp.[Method] Using ten species of Rosa spp.plants as experimental materials,different combinations of hormones were designed to establish...[Objective] This study was to develop an in vitro tissue culture system of Rosa spp.[Method] Using ten species of Rosa spp.plants as experimental materials,different combinations of hormones were designed to establish their in vitro tissue culture system with the stem segments as explants.[Result] All ten tested varieties germinated when the nodal segment explants were cultured on the sprouting medium MS＋ 0.5 mg/L BA ＋0.01 mg/L NAA and grew vigorous shoots,and the sprouting rate was up to 70%.Of the ten tested rose varieties,each has a respective optimal proliferation medium,and the multiplication rates for all the varieties reached 3.0%.The axillary buds were vigorous and normal in leaf color.The optimal medium for rooting and acclimation was 1/2MS medium containing 0.1 mg/L or 0.2 mg/L NAA,in which the rooting frequency reached 90%-100% and the root system was developed.After acclimation and transplant,the survival rate was as high as 95%.[Conclusion] An in vitro tissue culture system of Rosa spp.has been established in this study,which lays foundation for the molecular breeding of Rosa spp.展开更多
Plant tissue culture systems have enormous potential in fundamental re- search and for commercial applications such as horticultural industry. The process of tissue culture is companied with a series of changes in res...Plant tissue culture systems have enormous potential in fundamental re- search and for commercial applications such as horticultural industry. The process of tissue culture is companied with a series of changes in respect to morphology, physiology, biochemistry, molecule and epigenetics. The changes at molecule levels mainly include genetic variation, DNA sequence variation, chromosomal variation and epigenetic regulation （DNA methylation, histone modification, chromatin remodeling, small RNA regulation）. These changes are believed to facilitate explant adaptation to culture conditions and to help subsequent morphogenesis processes. Nowadays, it has played a crucial part in commercial applications and in basic research into cell biology, genetics and biochemistry, etc. In present review, we shed light on the fun- damental of plant tissue culture, culture medium preparation, explant selection, mechanism of action of various hormones, the three major problems （explant pollu- tion, browning, plantlets vitrification） and the prevention measures in tissue culture, and elaborated on in vitro propagation of plants, virus-free seedling cultivation, cry- opreservation, artificial seeds and molecule levels changes during in vitro culture further.展开更多
This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plant...This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plants and seeds for tube germination,two types of plants regeneration（organ type and organ genesis type）,methods of rooting and transplanting,factors affecting culture,as well as browning and vitrification phenomena and avoiding measures.And the further biotechnology research fields of E.mollis were prospected.展开更多
Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carri...Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.展开更多
Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic ...Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor.The cells of calli were still vigorous when treated with 0.75 mol/L hyperosmotic solution for 4 hours.The differentiation rates of calli varied from 7.5% to 93.3% in different media.The bud differentiation was apparently inhibited by hyperosmotic treatments.0.75mol/L sucrose hypertonic solution and 0.2mol/L CaCl2 solution were favorable hyperosmoticum and hypcosmoticum respectively.展开更多
Plant tissue culture continues to be of great interest within the realms of molecular biology, plant breeding and plant health However, different plant cultivars have different culture efficiencies to tissue culture. ...Plant tissue culture continues to be of great interest within the realms of molecular biology, plant breeding and plant health However, different plant cultivars have different culture efficiencies to tissue culture. In this research, the response of two Kenyan sweet potato varieties, KEMB 36 and Tainurey, cultured on a low cost tissue culture medium was evaluated. The low cost medium contained plant nutrients that were obtained from locally available fertilizers. Each conventional Murashige and Skoog （MS） macronutrient was individually substituted with a locally available fertilizer. The conventional source of micronutrients was substituted with Stanes~ Iodized Microfood while sucrose was obtained from table sugar. Performance of the two cultivars was monitored over a period of six weeks. KEMB 36 had a better performance than Tainurey with an average of eight nodes, seven leaves, three roots and height of four centimeters per plantlet indicating genotype-dependent response.展开更多
This study aims at determining and comparing the in vitro antifungal activity of six EOs （essential oils） extracted from Monodora myristica, Melaleuca quinquenervia, Eucalyptus torelliana, Chenopodium ambrosioides, ...This study aims at determining and comparing the in vitro antifungal activity of six EOs （essential oils） extracted from Monodora myristica, Melaleuca quinquenervia, Eucalyptus torelliana, Chenopodium ambrosioides, fresh Zingiber officinalis and dehydrated Zingiber officinalis with those of three synthetic fungicides （Callicuivre, Banko plus and Mancozeb） on Sclerotium rolfsii a pathogenic fungus of tomato in Cote d＇Ivoire. The products were added to PDA （potato dextrose agar） medium at various concentrations. The results showed different aspects of the mycelium, different activities on mycelium growth and sclerotia production depending on the explants （mycelium or sclerotia）. Depending on their IC and MGI （mycelium growth inhibition）, the oils stemming from Chenopodium ambrosioides and fresh Zingiber officinalis showed more inhibitory activities on mycelial growth （reduction rate of 100%） and sclerotia production at 250 ppm. These activities were close to or even greater than those of fungicides. The mancozeb fungicide showed stronger activity than the others by inhibiting mycelium growth and sclerotia production of mycelium explants at 250 ppm. This study offers the possibility of using EOs in the control strategies of Sclerotium rolfsii.展开更多
The present status of studies on regeneration of tomato in vitro was introduced, and the key factors that influence the shoot regeneration system in tomato were summarized, such as genotypes, explants type, explant ph...The present status of studies on regeneration of tomato in vitro was introduced, and the key factors that influence the shoot regeneration system in tomato were summarized, such as genotypes, explants type, explant physiological status, plant hormones and additives. The paper also discussed issues existing in tomato tissue culture and prospected the future development of this system.展开更多
An efficient protocol was developed for direct plant regeneration, multiplication and rooting of Balsam pear in vitro. The key factors influencing regeneration such as genotypes, explants type, seedling age, plant hor...An efficient protocol was developed for direct plant regeneration, multiplication and rooting of Balsam pear in vitro. The key factors influencing regeneration such as genotypes, explants type, seedling age, plant hormones and additives were summarized. The paper also discussed the problems existing on balsam pear tissue culture and prospected the future development of this system.展开更多
文摘[ Objective] The study was to compare the structural and moisture characteristics of leaf from the plantlets of three types of omamental lily（ Lilium brownii）. [ Method ] The paraffin sections of leaves of tested lily varieties were prepared and then observed under microscope, and the stomatal characteristics and moisture characteristics of tested lily varieties were measured. I Resaltl All the three ornamental lily varieties show isobilateral leaf, single layer of epicuticula and lower epidermis, and no obvious differentiation of palisade tissue and spongy tissue; their stomata distribute in lower epidermis, and the guard cells are dumbbell-shaped; all of these matedais present high moisture. For the leaf sick- ness, midrib sickness and mesophyll tissue sickness, the order was determined to be oriental lily 〉 Lilium/ongiflorum 〉 Asian lily; of the three types of ornamental lily, Ulium Iongiflorum has the largest stomatai aperture and Asian lily has the smallest; focusing the water potential and moisture, the turn was Asian lily 〉 oriental lily 〉 Lilium Iong＇fflorum. [ Condusion] The study may facilitate the artificial regulation of the growth conditions of the plantlets of ornamental lily.
基金Supported by National Natural Science Foundation of China(30900158)Research Foundation for Guangdong Pharmaceutical University(43553006)~~
文摘[Objective] The study had developed a means of rapid propagation Pteris vittata L.by tissue culture. The species was a perennial fern belonging to the genus Pteris. [Metbed] The leaf bud of P. vittata collected in field conditions as explantsand the 1/2 MS ＋ 3% sucrose ＋ 0.7% agar as the basic medium were used to screen the medium formula of the phytohormone ratio for callus induction and subculture of P. vittata. [Result] The best medium formula for each step was list below： 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.1 mg/L KT ＋ 0.5 mg/L 2, 4-D for in- ducing the callus from explants; 1/2MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 1.0 mg/L KT ＋ 0.01 mg/L 2,4-D for inducing the GGB from callus and the seedlings from GGB. In addition, 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.5 mg/L 2,4-D for the subculture could make the continued proliferation of callus. [Cen- clusioa] This study makes an applicable procedure by the direct use of field materi- als, for propagating P. vittata in a simplified and rapid mode.
文摘This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.
基金Supported by National Natural Science Foundation of China(30871733)~~
文摘[Objective] This study was to develop an in vitro tissue culture system of Rosa spp.[Method] Using ten species of Rosa spp.plants as experimental materials,different combinations of hormones were designed to establish their in vitro tissue culture system with the stem segments as explants.[Result] All ten tested varieties germinated when the nodal segment explants were cultured on the sprouting medium MS＋ 0.5 mg/L BA ＋0.01 mg/L NAA and grew vigorous shoots,and the sprouting rate was up to 70%.Of the ten tested rose varieties,each has a respective optimal proliferation medium,and the multiplication rates for all the varieties reached 3.0%.The axillary buds were vigorous and normal in leaf color.The optimal medium for rooting and acclimation was 1/2MS medium containing 0.1 mg/L or 0.2 mg/L NAA,in which the rooting frequency reached 90%-100% and the root system was developed.After acclimation and transplant,the survival rate was as high as 95%.[Conclusion] An in vitro tissue culture system of Rosa spp.has been established in this study,which lays foundation for the molecular breeding of Rosa spp.
基金Supported by Natural Science Foundation of Inner Mongolia(2012MS0516)~~
文摘Plant tissue culture systems have enormous potential in fundamental re- search and for commercial applications such as horticultural industry. The process of tissue culture is companied with a series of changes in respect to morphology, physiology, biochemistry, molecule and epigenetics. The changes at molecule levels mainly include genetic variation, DNA sequence variation, chromosomal variation and epigenetic regulation （DNA methylation, histone modification, chromatin remodeling, small RNA regulation）. These changes are believed to facilitate explant adaptation to culture conditions and to help subsequent morphogenesis processes. Nowadays, it has played a crucial part in commercial applications and in basic research into cell biology, genetics and biochemistry, etc. In present review, we shed light on the fun- damental of plant tissue culture, culture medium preparation, explant selection, mechanism of action of various hormones, the three major problems （explant pollu- tion, browning, plantlets vitrification） and the prevention measures in tissue culture, and elaborated on in vitro propagation of plants, virus-free seedling cultivation, cry- opreservation, artificial seeds and molecule levels changes during in vitro culture further.
基金Supported by Scientific and Technological Development Project of Education Department in Shanxi(606048)~~
文摘This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plants and seeds for tube germination,two types of plants regeneration（organ type and organ genesis type）,methods of rooting and transplanting,factors affecting culture,as well as browning and vitrification phenomena and avoiding measures.And the further biotechnology research fields of E.mollis were prospected.
基金Supported by the National Natural Science Foundation of China Granted Project(81173484)
文摘Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.
文摘Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor.The cells of calli were still vigorous when treated with 0.75 mol/L hyperosmotic solution for 4 hours.The differentiation rates of calli varied from 7.5% to 93.3% in different media.The bud differentiation was apparently inhibited by hyperosmotic treatments.0.75mol/L sucrose hypertonic solution and 0.2mol/L CaCl2 solution were favorable hyperosmoticum and hypcosmoticum respectively.
文摘Plant tissue culture continues to be of great interest within the realms of molecular biology, plant breeding and plant health However, different plant cultivars have different culture efficiencies to tissue culture. In this research, the response of two Kenyan sweet potato varieties, KEMB 36 and Tainurey, cultured on a low cost tissue culture medium was evaluated. The low cost medium contained plant nutrients that were obtained from locally available fertilizers. Each conventional Murashige and Skoog （MS） macronutrient was individually substituted with a locally available fertilizer. The conventional source of micronutrients was substituted with Stanes~ Iodized Microfood while sucrose was obtained from table sugar. Performance of the two cultivars was monitored over a period of six weeks. KEMB 36 had a better performance than Tainurey with an average of eight nodes, seven leaves, three roots and height of four centimeters per plantlet indicating genotype-dependent response.
文摘This study aims at determining and comparing the in vitro antifungal activity of six EOs （essential oils） extracted from Monodora myristica, Melaleuca quinquenervia, Eucalyptus torelliana, Chenopodium ambrosioides, fresh Zingiber officinalis and dehydrated Zingiber officinalis with those of three synthetic fungicides （Callicuivre, Banko plus and Mancozeb） on Sclerotium rolfsii a pathogenic fungus of tomato in Cote d＇Ivoire. The products were added to PDA （potato dextrose agar） medium at various concentrations. The results showed different aspects of the mycelium, different activities on mycelium growth and sclerotia production depending on the explants （mycelium or sclerotia）. Depending on their IC and MGI （mycelium growth inhibition）, the oils stemming from Chenopodium ambrosioides and fresh Zingiber officinalis showed more inhibitory activities on mycelial growth （reduction rate of 100%） and sclerotia production at 250 ppm. These activities were close to or even greater than those of fungicides. The mancozeb fungicide showed stronger activity than the others by inhibiting mycelium growth and sclerotia production of mycelium explants at 250 ppm. This study offers the possibility of using EOs in the control strategies of Sclerotium rolfsii.
文摘The present status of studies on regeneration of tomato in vitro was introduced, and the key factors that influence the shoot regeneration system in tomato were summarized, such as genotypes, explants type, explant physiological status, plant hormones and additives. The paper also discussed issues existing in tomato tissue culture and prospected the future development of this system.
文摘An efficient protocol was developed for direct plant regeneration, multiplication and rooting of Balsam pear in vitro. The key factors influencing regeneration such as genotypes, explants type, seedling age, plant hormones and additives were summarized. The paper also discussed the problems existing on balsam pear tissue culture and prospected the future development of this system.
