Lysine-rich protein gene (lys) was cloned from Psophocarpus tetragonolobus (L.) DC. A plant expression plasmid was constructed and lys gene was under the control of maize ubiquitin promoter which is the highest effici...Lysine-rich protein gene (lys) was cloned from Psophocarpus tetragonolobus (L.) DC. A plant expression plasmid was constructed and lys gene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter. The plasmid was introduced into rice embryogenic calli by microprojectile bombardment. The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. Genomic PCR and Southern blotting analyses showed that the lys gene has been integrated into rice genome. Simultaneously, the results of GUS histochemical assay demonstrated that gus report gene is also expressed in leaves, stems and roots of the transgenic rice plants. Data analysis showed that lysine content in most of the 11 transgenic plants is differently improved, and in one of them increased by 16.04%.展开更多
C-14-glutamate and C-14-arginine were spreaded on leaves of six-day old barley (Hordeum vulgare L.) seedlings that were treated with NaCl 200 mmol/L. The result showed that the pathway of arginine-->ornithine-->...C-14-glutamate and C-14-arginine were spreaded on leaves of six-day old barley (Hordeum vulgare L.) seedlings that were treated with NaCl 200 mmol/L. The result showed that the pathway of arginine-->ornithine-->proline existed in the six-day old barley seedlings and was provoked remarkably by NaCl treatment. After seven days, proline accumulation contributed via the arginine-->ornithine-->proline pathway was 1.0 - 1.5 folds of that via the glutamate-->proline pathway. The activation of arginine-->ornithine-->proline pathway by salt stress in the salt-tolerant cultivar 'Jian 4' was 1.7 - 2.0 folds of that in the salt-sensitive cultivar 'KP 7', which suggested that the activation of arginine-->ornithine-->proline pathway in barley seedlings played an important role in improving salt tolerance of plants.展开更多
Mg2+ and PO43+ were added into the synthetic wastewater, leading to the dissociation of the complex ions in the wastewater, and resulting in removal of copper and ammonia therein. The effects of agents addition amo...Mg2+ and PO43+ were added into the synthetic wastewater, leading to the dissociation of the complex ions in the wastewater, and resulting in removal of copper and ammonia therein. The effects of agents addition amount, pH, and reaction time on the removal efficiency of copper and ammonia were investigated. In particular, two-sectional struvite formation (TSSF) process was established for copper and ammonia removal. MgCl2 and Na2HPO4 were added by following 90% addition in the first section and remained 10% in the second during the TSSF process. Compared with one sectional struvite formation, TSSF possessed much better performance. Under condition of n(NH3-N):n(Mg):n(P)=1:1.2:1.5 (molar ratio), pH=9, and reaction time of 30 min, the removal efficiencies of copper and ammonia were 98.9% and 99.96%, respectively. The enhanced performance of TSSF is explained by the competition of ammonia by copper?ammonia complexes and struvite. The dissociation of copper-ammonia complexes is further demonstrated by thermodynamic equilibrium analysis, on the basis of calculations and establishment of predominance phases diagram. Moreover, XRD and EDS analyses further confirmed the formation of struvite and precipitation of copper, which prove the transmission of copper and ammonia from liquid phase into solid phase.展开更多
High-purity magnesium ammonium phosphate (MAP) was precipitated by controlling pH value of the reaction system of 9.0-9.5. The thermal decomposition behavior of MAP and the adsorption properties of its pyrolysis pro...High-purity magnesium ammonium phosphate (MAP) was precipitated by controlling pH value of the reaction system of 9.0-9.5. The thermal decomposition behavior of MAP and the adsorption properties of its pyrolysis products toward ammonia-nitrogen were also studied by XRD, SEM, TGA-DTA and FT-IR methods. The results indicated that high-purity MAP was obtained at pH value of 9.0-9.5. Upon heating to 100-120℃ for 120 min, MAP was thermally decomposed, losing water and ammonia concomitantly with a reduction in grain size and crystallinity. The capacity of pyrolysis products for ammonia nitrogen adsorption reached 72.5 mg/g, with a removal rate of up to 95% from an 800 mg/L solution. The characteristic diffraction peaks corresponding to MAP mainly appeared in their XRD patterns after adsorption of ammonia nitrogen. The pyrolysis products of MAP at 100-120 ℃ could be recycling-used as the chemical treatment regents of ammonia nitrogen in the practical application.展开更多
Porous graphitic carbon nitride(pg-C3N4) nanosheets have been prepared through a one-step ammonia thermopolymerization method.The effects of synthetic temperature on the structural,optical and photocatalytic propert...Porous graphitic carbon nitride(pg-C3N4) nanosheets have been prepared through a one-step ammonia thermopolymerization method.The effects of synthetic temperature on the structural,optical and photocatalytic properties of the samples have been investigated.Characterization results show that the heptazine-based conjugate heterocyclic structure was formed over 500℃,which is attributed to the inhibitory effect of ammonia from the decomposition of NH4SCN.Precise nanosheet morphology and an increased pore distribution with an enlarged surface area are observed for the samples obtained under high temperatures.Optical analysis results show that the bandgap of the samples widens and photoluminescene intensity is gradually quenched as the treating temperature is increased.The results demonstrate that a higher polymerization temperature improves the nanolayer structure,porosity and migration rate of the photo-induced carriers of the samples.The pg-C3N4 nanosheets prepared at 600℃ presents the highest photocatalytic activity for hydrogen evolution from water under visible-light irradiation.This study demonstrates a novel strategy for the synthesis and optimization of polymer semiconductor nanosheets with gratifying photocatalytic performance.展开更多
In order to achieve the dual goals of complete deamination of magnesium ammonium phosphate(MAP) and ensure the pyrolysate's good removal properties towards ammonia-nitrogen, a temperature-programmed method for the ...In order to achieve the dual goals of complete deamination of magnesium ammonium phosphate(MAP) and ensure the pyrolysate's good removal properties towards ammonia-nitrogen, a temperature-programmed method for the pyrolysis of MAP was studied, as well as the thermodynamic and kinetic processes involved in the removal reaction system between MAP pyrolysate and aqueous ammonium. It was found that the pyrolysis method and pyrolysis final temperature had significant effects on the MAP pyrolysate's removal properties towards aqueous ammonium, and the following conditions were deemed to be more appropriate: pyrolysis final temperature and heating rate at 180 ℃ and 5 ℃/min, respectively, and a pH level of 9.5 for the removal reaction system. The resultant ammonium removal capacity by the MAP pyrolysate was 95.62 mg/g. After 120 min, the removal rate with an initial concentration of 1000 mg/L was 82%. The kinetic and thermodynamic results indicated that the removal of aqueous ammonium by MAP pyrolysate was the exchange process between H^+ and NH4^+ via MAP precipitation. The kinetics complied with the Lagergren quasi second-order model with an equilibrium time of 120 min, while the isothermal curves complied with the Freundlich model.展开更多
A pot experiment was conducted to study the influences of foliar application of glycine,alanine,lysine,and glutamic acid in 200 mg/kg or 500 mg/kg upon the quality and enzyme activity of flowering Chinese cabbage(Bra...A pot experiment was conducted to study the influences of foliar application of glycine,alanine,lysine,and glutamic acid in 200 mg/kg or 500 mg/kg upon the quality and enzyme activity of flowering Chinese cabbage(Brassica parachinensis Bailey).The results showed that all the application of these four amino acids could increase the yield of flowering Chinese cabbage,significantly raise the content of soluble sugar,and reduce the accumulation of nitrate.The applications of three other amino acids except alanine can increase the content of soluble proteins and decrease the accumulation of oxalic acid.However,the application of amino acid has insignificant influences on the SPAD number of chlorophyll,and causes the decrease of Vitamin C content.Meanwhile,the application of amino acid can improve the activity of nitrate reductase(NR) and glutamate dehydrogenase(GDH) as well.It shows that the application of amino acid is beneficial to improve ammonia metabolism,reduce the accumulation of nitrate and oxalic acid,increase the content of soluble sugar and soluble proteins,and improve the quality of flowering Chinese cabbage.展开更多
Recent studies show that a reduced effect of inhibitory transmitter system in the visual cortex may underlie aged visual function degradation. Whether excitatory transmitter system changes with age and hence affects i...Recent studies show that a reduced effect of inhibitory transmitter system in the visual cortex may underlie aged visual function degradation. Whether excitatory transmitter system changes with age and hence affects intracortical excitation-inhibition balance is not clear. To explore this issue, we used Nissl staining and immunohistochemical methods as well as Image-Pro Express software to examine the density of Nissl-stained neurons, Glutamie acid-immunoreactive (Glu-IR) neurons and T-Aminobutyric acid-immunoreactive (GABA-IR) neurons in the primary visual cortex of young adult and aged cats. The results showed that there was no significant difference in the density of Nissl-stained neurons between young and old cats (2〉0.05). However, the density of Glu-IR neurons and GABA-IR neurons in the primary visual cortex of aged cats was significantly lower than that of young ones (P〈0.01). The ratio between Glu-IR neurons and GABA-IR neurons was significantly increased in old cats compared to that in young adult ones (P〈0.01). These results indicated that the effect of excitatory transmitter system in the old visual cortex was increased relative to the inhibitory transmitter system, which might cause an imbalance between cortical excitation and inhibition and might be an important factor mediating the visual function decline during aging.展开更多
The extraction behaviors of zinc from ammoniacal solutions were investigated using β-diketone (HA) and their mixtures with CYANEX923 or LIX84I. The effects of pH, total ammonia concentration, extractant concentrati...The extraction behaviors of zinc from ammoniacal solutions were investigated using β-diketone (HA) and their mixtures with CYANEX923 or LIX84I. The effects of pH, total ammonia concentration, extractant concentration, anion species and temperature on zinc extraction were examined. The synergistic mechanism was discussed with regard to the structure of extractant and the extracted zinc complexes. It is found that the increase of total ammonia concentration and pH inhibits zinc extraction for all extraction systems due to the formation of zinc ammine complexes in aqueous phase. This effect of HA with CYANEX923 is evidently smaller than that of HA with LIX84I or HA alone system. Effect of anion species on the zinc extraction by HA with CYANEX923 can be neglected, but this effect of HA alone and the mixture of HA with LIX84I decreases in the order of (NH4)2SO4 〉 NH4NO3 〉 NH4Cl.展开更多
Amino-bacterial cellulose(amino-BC) was prepared by chemical modification of bacterial cellulose(BC).The adsorption characteristics and mechanism of amino-BC were studied.The results show that adsorption data can ...Amino-bacterial cellulose(amino-BC) was prepared by chemical modification of bacterial cellulose(BC).The adsorption characteristics and mechanism of amino-BC were studied.The results show that adsorption data can be fitted well by Langmuir equation and the pseudo-second order kinetics,indicating that the adsorption of amino-BC would obey monolayer molecule adsorption and the main action was chemisorption.Meanwhile,the adsorption process was studied by the Elovich equation and the intra-particle diffusion model,indicating that the absorption characteristics of metal ions on amino-BC is controlled by both film diffusion and particle diffusion.The increase of reaction temperature will accelerate the adsorbing rate because of endothermic reaction.展开更多
A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase (LOX), a key enzyme ofjasmonate (JA) pathway, and phenylalanine ammonia lya...A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase (LOX), a key enzyme ofjasmonate (JA) pathway, and phenylalanine ammonia lyase (PAL), a rate-limiting enzyme of phenyl- propanoid pathway, were measured in the leaves of one-year-old poplar (Populus simonii × P. pyramidalis 'Opera 8277') cuttings after Clostera anachoreta larvae attack. The results show that the increased activities of LOX and PAL were found not only in the leaves wounded by C. anachoreta larvae but also in their tipper systemic leaves, indicating that JA and phenylpropanoid pathways were activated, and the defense response was stimulated systemically. The increase in LOX and PAL activities in neighboring intact poplar cuttings sug- gested that there exists the interplant communication between poplar plants mediated by the herbivore-induced volatiles. Methyl jasmonate (MeJA) was also proved to be an airborne signal to induce defense response in P simonii × P pyramidalis 'Opera 8277' cuttings.展开更多
ZSM-5 zeolites were directly synthesized on the surface of honeycomb cordierite substrates by hydrothermal method and certified by XRD and SEM techniques; the adhesion of ZSM-5 coatings was evaluated by ultrasonic vib...ZSM-5 zeolites were directly synthesized on the surface of honeycomb cordierite substrates by hydrothermal method and certified by XRD and SEM techniques; the adhesion of ZSM-5 coatings was evaluated by ultrasonic vibration. Cu-ZSM-5/cordierite monolithic catalyst was prepared by ion-exchange and impregnation method and applied for the selective catalytic reduction (SCR) of NO by NH3 using a simulated diesel exhaust. The results show that the cordierite surface is almost completely covered by ZSM-5 crystals and the crystallization time greatly impacts the loadings and adhesion of ZSM-5 coatings on substrate, the NOx removal rate over Cu-ZSM-5/cordierite is above 90% in a temperature range of 240-480℃. Moreover, Cu-ZSM-5/cordierite prepared by different methods shows a wide temperature window (240-540 ℃) with high NO removal activities.展开更多
Amino-functionalized magnetic nanoparticle (NH2-MNP) were prepared by a sol-gel approach. The adsorption behavior of Cu(II) ions on NH2-MNP was discussed systematically by batch experiments. The effects of initial...Amino-functionalized magnetic nanoparticle (NH2-MNP) were prepared by a sol-gel approach. The adsorption behavior of Cu(II) ions on NH2-MNP was discussed systematically by batch experiments. The effects of initial Cu(II) ions concentration, time, pH and temperature were investigated. In kinetic studies, the pseudo-second-order model was successfully employed, and the pseudo-first-order model substantiated that Cu(II) adsorption on NH2-MNP was a diffusion-based process. Langmuir model and Dubinin-Radushkevich model (R2〉0.99) were more corresponded with the adsorption isotherm data of Cu(II) ions than Freundlich model. The adsorption capacity was increased with the increment of temperature and pH. NH2-MNP remains excellent Cu(II) recoveries after reusing five adsorption and desorption cycles, making NH2-MNP a promising candidate for repetitively removing heavy metal ions from environmental water samples. According to the results obtained from adsorption activation energy and thermodynamic studies, it can be inferred that the main adsorption mechanism between absorbent and Cu(II) ions is ion exchange-surface complexation.展开更多
[Objective] The aim of this study was to provide metabolic evidence for the analysis of the ecological and safety assessment of Pi-d2-transgenic rice.[Method] The main agronomic characters of Pi-d2-transgenic rice wer...[Objective] The aim of this study was to provide metabolic evidence for the analysis of the ecological and safety assessment of Pi-d2-transgenic rice.[Method] The main agronomic characters of Pi-d2-transgenic rice were observed in field experiment and the grain chemical characters and amino acid content were measured.[Results] Introduction of foreign gene Pi-d2 resulted in stably hereditable variation in agronomic characteristics in the descents.Most of the transgenic lines grew normally and orderly.Compared with the control(wild type plants),about half of transgenic plants showed an increased or reduced plant height.There was no observable difference between transgenic plants and controls in tiller number,length of panicle,panicles per plant,seed-setting rate and 1 000-grain weight.Total amino acid content in transgenic rice was reduced,while the starch content,GC and GT were not altered in comparison with the control.[Conclusion] Introduction of foreign gene Pi-d2 has remarkable influence on plant height,while little on grain chemical characters.展开更多
文摘Lysine-rich protein gene (lys) was cloned from Psophocarpus tetragonolobus (L.) DC. A plant expression plasmid was constructed and lys gene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter. The plasmid was introduced into rice embryogenic calli by microprojectile bombardment. The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. Genomic PCR and Southern blotting analyses showed that the lys gene has been integrated into rice genome. Simultaneously, the results of GUS histochemical assay demonstrated that gus report gene is also expressed in leaves, stems and roots of the transgenic rice plants. Data analysis showed that lysine content in most of the 11 transgenic plants is differently improved, and in one of them increased by 16.04%.
文摘C-14-glutamate and C-14-arginine were spreaded on leaves of six-day old barley (Hordeum vulgare L.) seedlings that were treated with NaCl 200 mmol/L. The result showed that the pathway of arginine-->ornithine-->proline existed in the six-day old barley seedlings and was provoked remarkably by NaCl treatment. After seven days, proline accumulation contributed via the arginine-->ornithine-->proline pathway was 1.0 - 1.5 folds of that via the glutamate-->proline pathway. The activation of arginine-->ornithine-->proline pathway by salt stress in the salt-tolerant cultivar 'Jian 4' was 1.7 - 2.0 folds of that in the salt-sensitive cultivar 'KP 7', which suggested that the activation of arginine-->ornithine-->proline pathway in barley seedlings played an important role in improving salt tolerance of plants.
基金Project(51674305)supported by the National Natural Science Foundation of ChinaProject(2013WK2007)supported by the Key Project of Science and Technology of Hunan Province,China+1 种基金Project(2015CX001)supported by the Innovation Stimulating Program of Central South University,ChinaKey Project(1602FKDC007)supported by Science and Technology Program of Gansu Province,China
文摘Mg2+ and PO43+ were added into the synthetic wastewater, leading to the dissociation of the complex ions in the wastewater, and resulting in removal of copper and ammonia therein. The effects of agents addition amount, pH, and reaction time on the removal efficiency of copper and ammonia were investigated. In particular, two-sectional struvite formation (TSSF) process was established for copper and ammonia removal. MgCl2 and Na2HPO4 were added by following 90% addition in the first section and remained 10% in the second during the TSSF process. Compared with one sectional struvite formation, TSSF possessed much better performance. Under condition of n(NH3-N):n(Mg):n(P)=1:1.2:1.5 (molar ratio), pH=9, and reaction time of 30 min, the removal efficiencies of copper and ammonia were 98.9% and 99.96%, respectively. The enhanced performance of TSSF is explained by the competition of ammonia by copper?ammonia complexes and struvite. The dissociation of copper-ammonia complexes is further demonstrated by thermodynamic equilibrium analysis, on the basis of calculations and establishment of predominance phases diagram. Moreover, XRD and EDS analyses further confirmed the formation of struvite and precipitation of copper, which prove the transmission of copper and ammonia from liquid phase into solid phase.
基金Project(ZDSY20120619093952884)supported by Shenzhen Strategic New Industry Development,China
文摘High-purity magnesium ammonium phosphate (MAP) was precipitated by controlling pH value of the reaction system of 9.0-9.5. The thermal decomposition behavior of MAP and the adsorption properties of its pyrolysis products toward ammonia-nitrogen were also studied by XRD, SEM, TGA-DTA and FT-IR methods. The results indicated that high-purity MAP was obtained at pH value of 9.0-9.5. Upon heating to 100-120℃ for 120 min, MAP was thermally decomposed, losing water and ammonia concomitantly with a reduction in grain size and crystallinity. The capacity of pyrolysis products for ammonia nitrogen adsorption reached 72.5 mg/g, with a removal rate of up to 95% from an 800 mg/L solution. The characteristic diffraction peaks corresponding to MAP mainly appeared in their XRD patterns after adsorption of ammonia nitrogen. The pyrolysis products of MAP at 100-120 ℃ could be recycling-used as the chemical treatment regents of ammonia nitrogen in the practical application.
基金supported by the National Natural Science Foundation of China(21503096)the Natural Science Foundation of Jiangsu Province(BK20140507)~~
文摘Porous graphitic carbon nitride(pg-C3N4) nanosheets have been prepared through a one-step ammonia thermopolymerization method.The effects of synthetic temperature on the structural,optical and photocatalytic properties of the samples have been investigated.Characterization results show that the heptazine-based conjugate heterocyclic structure was formed over 500℃,which is attributed to the inhibitory effect of ammonia from the decomposition of NH4SCN.Precise nanosheet morphology and an increased pore distribution with an enlarged surface area are observed for the samples obtained under high temperatures.Optical analysis results show that the bandgap of the samples widens and photoluminescene intensity is gradually quenched as the treating temperature is increased.The results demonstrate that a higher polymerization temperature improves the nanolayer structure,porosity and migration rate of the photo-induced carriers of the samples.The pg-C3N4 nanosheets prepared at 600℃ presents the highest photocatalytic activity for hydrogen evolution from water under visible-light irradiation.This study demonstrates a novel strategy for the synthesis and optimization of polymer semiconductor nanosheets with gratifying photocatalytic performance.
基金Project(ZDSY20120619093952884)supported by Shenzhen Strategic New Industry Development,China
文摘In order to achieve the dual goals of complete deamination of magnesium ammonium phosphate(MAP) and ensure the pyrolysate's good removal properties towards ammonia-nitrogen, a temperature-programmed method for the pyrolysis of MAP was studied, as well as the thermodynamic and kinetic processes involved in the removal reaction system between MAP pyrolysate and aqueous ammonium. It was found that the pyrolysis method and pyrolysis final temperature had significant effects on the MAP pyrolysate's removal properties towards aqueous ammonium, and the following conditions were deemed to be more appropriate: pyrolysis final temperature and heating rate at 180 ℃ and 5 ℃/min, respectively, and a pH level of 9.5 for the removal reaction system. The resultant ammonium removal capacity by the MAP pyrolysate was 95.62 mg/g. After 120 min, the removal rate with an initial concentration of 1000 mg/L was 82%. The kinetic and thermodynamic results indicated that the removal of aqueous ammonium by MAP pyrolysate was the exchange process between H^+ and NH4^+ via MAP precipitation. The kinetics complied with the Lagergren quasi second-order model with an equilibrium time of 120 min, while the isothermal curves complied with the Freundlich model.
基金Supported by National Scientific and Technological Supporting Project(2008BADA4B04-09)Guangdong Province Scientific and Technological Project(2008A020100017)Guangdong Province Department of Finance Project[(2006)143]~~
文摘A pot experiment was conducted to study the influences of foliar application of glycine,alanine,lysine,and glutamic acid in 200 mg/kg or 500 mg/kg upon the quality and enzyme activity of flowering Chinese cabbage(Brassica parachinensis Bailey).The results showed that all the application of these four amino acids could increase the yield of flowering Chinese cabbage,significantly raise the content of soluble sugar,and reduce the accumulation of nitrate.The applications of three other amino acids except alanine can increase the content of soluble proteins and decrease the accumulation of oxalic acid.However,the application of amino acid has insignificant influences on the SPAD number of chlorophyll,and causes the decrease of Vitamin C content.Meanwhile,the application of amino acid can improve the activity of nitrate reductase(NR) and glutamate dehydrogenase(GDH) as well.It shows that the application of amino acid is beneficial to improve ammonia metabolism,reduce the accumulation of nitrate and oxalic acid,increase the content of soluble sugar and soluble proteins,and improve the quality of flowering Chinese cabbage.
基金Natural Science Fund of Anhui Province (070413138)Key Laboratory Foundation of Anhui Province for Researches on the Conservation and Utilization of Important Biological ResourceKey Laboratory Foundation for Universities and Colleges in Anhui
文摘Recent studies show that a reduced effect of inhibitory transmitter system in the visual cortex may underlie aged visual function degradation. Whether excitatory transmitter system changes with age and hence affects intracortical excitation-inhibition balance is not clear. To explore this issue, we used Nissl staining and immunohistochemical methods as well as Image-Pro Express software to examine the density of Nissl-stained neurons, Glutamie acid-immunoreactive (Glu-IR) neurons and T-Aminobutyric acid-immunoreactive (GABA-IR) neurons in the primary visual cortex of young adult and aged cats. The results showed that there was no significant difference in the density of Nissl-stained neurons between young and old cats (2〉0.05). However, the density of Glu-IR neurons and GABA-IR neurons in the primary visual cortex of aged cats was significantly lower than that of young ones (P〈0.01). The ratio between Glu-IR neurons and GABA-IR neurons was significantly increased in old cats compared to that in young adult ones (P〈0.01). These results indicated that the effect of excitatory transmitter system in the old visual cortex was increased relative to the inhibitory transmitter system, which might cause an imbalance between cortical excitation and inhibition and might be an important factor mediating the visual function decline during aging.
基金Project (2007CB613601) supported by the National Basic Research Program of ChinaProject (CX2010B112) supported by Hunan Provincial Innovation Foundation for Postgraduate, China
文摘The extraction behaviors of zinc from ammoniacal solutions were investigated using β-diketone (HA) and their mixtures with CYANEX923 or LIX84I. The effects of pH, total ammonia concentration, extractant concentration, anion species and temperature on zinc extraction were examined. The synergistic mechanism was discussed with regard to the structure of extractant and the extracted zinc complexes. It is found that the increase of total ammonia concentration and pH inhibits zinc extraction for all extraction systems due to the formation of zinc ammine complexes in aqueous phase. This effect of HA with CYANEX923 is evidently smaller than that of HA with LIX84I or HA alone system. Effect of anion species on the zinc extraction by HA with CYANEX923 can be neglected, but this effect of HA alone and the mixture of HA with LIX84I decreases in the order of (NH4)2SO4 〉 NH4NO3 〉 NH4Cl.
基金Project (20130206059G X) supported by Science and Technology Key Project of Jilin Province,ChinaProject (20101553) supported by the Natural Science Foundation of Jilin Province,China+1 种基金Project (BSJXM-201226) supported by Doctor Science Research Starting Projects of Northeast Dianli University,ChinaProject (2013) supported by the 12th Five-Year Enhancing Innovation Projects of Northeast Dianli University,China
文摘Amino-bacterial cellulose(amino-BC) was prepared by chemical modification of bacterial cellulose(BC).The adsorption characteristics and mechanism of amino-BC were studied.The results show that adsorption data can be fitted well by Langmuir equation and the pseudo-second order kinetics,indicating that the adsorption of amino-BC would obey monolayer molecule adsorption and the main action was chemisorption.Meanwhile,the adsorption process was studied by the Elovich equation and the intra-particle diffusion model,indicating that the absorption characteristics of metal ions on amino-BC is controlled by both film diffusion and particle diffusion.The increase of reaction temperature will accelerate the adsorbing rate because of endothermic reaction.
基金supported by the Pro-gramme for Changjiang Scholars and the Innovative Research Team in Universities of China (PCSIRT0607)by the National Natural Science Foundation of China (30871727+2 种基金 30872037)the National Key Project of Scientific and Technical Supporting Programmes Funded by the Ministry of Science & Technology of China (2006BAD01A15 2006BAD24B04)
文摘A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase (LOX), a key enzyme ofjasmonate (JA) pathway, and phenylalanine ammonia lyase (PAL), a rate-limiting enzyme of phenyl- propanoid pathway, were measured in the leaves of one-year-old poplar (Populus simonii × P. pyramidalis 'Opera 8277') cuttings after Clostera anachoreta larvae attack. The results show that the increased activities of LOX and PAL were found not only in the leaves wounded by C. anachoreta larvae but also in their tipper systemic leaves, indicating that JA and phenylpropanoid pathways were activated, and the defense response was stimulated systemically. The increase in LOX and PAL activities in neighboring intact poplar cuttings sug- gested that there exists the interplant communication between poplar plants mediated by the herbivore-induced volatiles. Methyl jasmonate (MeJA) was also proved to be an airborne signal to induce defense response in P simonii × P pyramidalis 'Opera 8277' cuttings.
基金Project (20906067) supported by the National Natural Science Foundation of China
文摘ZSM-5 zeolites were directly synthesized on the surface of honeycomb cordierite substrates by hydrothermal method and certified by XRD and SEM techniques; the adhesion of ZSM-5 coatings was evaluated by ultrasonic vibration. Cu-ZSM-5/cordierite monolithic catalyst was prepared by ion-exchange and impregnation method and applied for the selective catalytic reduction (SCR) of NO by NH3 using a simulated diesel exhaust. The results show that the cordierite surface is almost completely covered by ZSM-5 crystals and the crystallization time greatly impacts the loadings and adhesion of ZSM-5 coatings on substrate, the NOx removal rate over Cu-ZSM-5/cordierite is above 90% in a temperature range of 240-480℃. Moreover, Cu-ZSM-5/cordierite prepared by different methods shows a wide temperature window (240-540 ℃) with high NO removal activities.
基金Project(CXZZ11-0812)supported by Graduate Students Innovative Projects of Jiangsu Province,ChinaProject(J20122288)supported by Guizhou Provincial Natural Science Foundation of China+1 种基金Project(Y4110235)supported by Zhejiang Provincial Natural Science Foundation of ChinaProject(JKY2011008)supported by Fundamental Research Funds for the Central Universities,China
文摘Amino-functionalized magnetic nanoparticle (NH2-MNP) were prepared by a sol-gel approach. The adsorption behavior of Cu(II) ions on NH2-MNP was discussed systematically by batch experiments. The effects of initial Cu(II) ions concentration, time, pH and temperature were investigated. In kinetic studies, the pseudo-second-order model was successfully employed, and the pseudo-first-order model substantiated that Cu(II) adsorption on NH2-MNP was a diffusion-based process. Langmuir model and Dubinin-Radushkevich model (R2〉0.99) were more corresponded with the adsorption isotherm data of Cu(II) ions than Freundlich model. The adsorption capacity was increased with the increment of temperature and pH. NH2-MNP remains excellent Cu(II) recoveries after reusing five adsorption and desorption cycles, making NH2-MNP a promising candidate for repetitively removing heavy metal ions from environmental water samples. According to the results obtained from adsorption activation energy and thermodynamic studies, it can be inferred that the main adsorption mechanism between absorbent and Cu(II) ions is ion exchange-surface complexation.
基金Supported by Program for New Century Excellent Talents in University of Ministry of Education of China(NCET-04-0907)Program for Young Scholars in Breeding from Sichuan Provincial Department of Finance(2009QNJJ-D18)~~
文摘[Objective] The aim of this study was to provide metabolic evidence for the analysis of the ecological and safety assessment of Pi-d2-transgenic rice.[Method] The main agronomic characters of Pi-d2-transgenic rice were observed in field experiment and the grain chemical characters and amino acid content were measured.[Results] Introduction of foreign gene Pi-d2 resulted in stably hereditable variation in agronomic characteristics in the descents.Most of the transgenic lines grew normally and orderly.Compared with the control(wild type plants),about half of transgenic plants showed an increased or reduced plant height.There was no observable difference between transgenic plants and controls in tiller number,length of panicle,panicles per plant,seed-setting rate and 1 000-grain weight.Total amino acid content in transgenic rice was reduced,while the starch content,GC and GT were not altered in comparison with the control.[Conclusion] Introduction of foreign gene Pi-d2 has remarkable influence on plant height,while little on grain chemical characters.