《流感》:戏剧化灾难叙事中的人性救赎

瘟疫题材电影《流感》讲述了韩国盆塘地区暴发猪流感引发恐慌和灾难的故事。影片采用高度戏剧化的艺术手法讲述这一灾难故事,最典型体现便是贯穿始终的“最后一分钟营救”,具体表现为姜智久对美日的营救、金仁海对美日的营救、孟瑟对美日的营救和总统对民众的营救,密集而丰富的“最后一分钟营救”令影片自始至终都扣人心弦。《流感》通过戏剧化的灾难叙事,讴歌了人性善和人间爱,正是这人性至善和人间大爱拯救了深陷瘟疫灾难中的盆塘居民,帮助他们战胜猪流感瘟疫,重新迎来幸福美好的生活。在诸多瘟疫题材影片中,《流感》以其戏剧化的灾难叙事和环环相扣的情节设置而成为代表性佳作,但仍不可避免地存在叙事缺陷。

电影艺术中的“幻想世界”到人性灾难——以韩国灾难性电影《流感》为例

随着韩国电影模式的升级,从2006年的《汉江怪物》到2013年的《流感》,韩国灾难性电影取得了很大的进步。电影艺术创作者可以通过一个完全幻想的世界,使得现实世界中的利害关系、实际情况不复存在,使观众远离现实的情况,只沉迷于其所创造的"幻想世界"中,引起观众的共鸣。本文以《流感》为例,对韩国灾难电影的特色及人性书写进行深入的剖析。

《流感》:疫病灾难中的二律背反及爱与互助的人道表达

《流感》(金成洙,2013)讲述因偷渡客携带致命猪流感病毒导致韩国盆塘市陷入疫病灾难的情境中,消防队救援队员姜智久与女医生金仁海共同寻找病毒宿主和解救民众的故事。影片揭示了人类面对疫病灾难时,个人求生本能与身肩社会责任,国际政治制衡与民众迫切期待的复杂纠结语境中,人类生存与家国存亡的二律背反。基于灾难片类型成规的生动表达,真挚动人的大爱精神与团结互助的人道情怀最终战胜了致命病毒,进而见出无私奉献、和衷共济才是克难度险的最有力武器。无疑,这是《流感》给正在抗击新冠肺炎的当下世界所带来的启示。

菊蓝抗流感片治疗新型冠状病毒感染的药效物质及作用机制整合研究

目的通过网络药理学和现代药理基础分析菊蓝抗流感片(JLKLGT)治疗新型冠状病毒感染(COVID-19)的药效物质及作用机制。方法采用网络药理学和分子对接技术,从TCMSP、BATMAN-TCM数据库检索菊蓝抗流感片各药物的活性成分及其靶点信息,使用GeneCards、OMIM和DrugBank数据库检索COVID-19疾病靶点。采用Cytoscape 3.7.2软件构建“菊蓝抗流感片药物-活性成分-靶点”网络图,通过STRING在线平台构建蛋白互作网络。通过Metascape数据库进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路分析,推测其药效物质及作用机制。结果共筛选菊蓝抗流感片中52种活性成分,对应靶点468个,COVID-19疾病靶点1410个和交集靶点107个。推测槲皮素、阿司匹林、木犀草素、金合欢素、胆固醇、豆甾醇和胆酸为治疗COVID-19主要药效物质,TP53、RELA、AKT1、TNF、MAPK1、NFKB1和MAPK14为关键核心靶点。分子对接结果表明,主要药效物质与关键核心靶点、血管紧张素转化酶2(ACE2)结合构象稳定。结论菊蓝抗流感片通过多成分、多靶点、多通路发挥防治COVID-19的作用。

抗流感颗粒剂治疗表寒里热证流行性感冒临床观察

目的观察并评价抗流感颗粒剂治疗表寒里热证流行性感冒的临床疗效。方法将103例患者随机分为两组,治疗组口服抗流感颗粒剂,对照组予以西药抗病毒治疗。结果治疗组愈显率高于对照组,且退热效果优于对照组。结论抗流感颗粒剂治疗表寒里热证流感疗效显著,安全可靠。

比较视野中的疫病灾难片研究——以《感染列岛》与《流感》为例

本文以日本电影《感染列岛》与韩国电影《流感》为例,分析其对相似题材的不同艺术表达,在比较视野中探讨疫病灾难片的主要特征及其现实意义。两部影片的主题都是人类在灾难中的反思,不同之处在于前者重在彰显平民的价值和力量,后者侧重批判权威意志对平民价值的无视和抹杀。

Effect of Curcumin on Influenza Virus HIN1 and H3N2 in Vitro

Objective：To investigate the inhibitory effect of curcumin on influenza virus HIN1 and H3N2 in vitro, Methods：The directly killing role of cureumin extract in vitro to influenza virus type A subtype H1N1 and H3N2 was evaluated by the canine kidney cells （MDCK）, Results：The largest non toxic concentration of curcumin extract was 12, 5g/L and the effective inhibitory concentration to H1N1 and H3N2 was 6, 25G/1 AND 1,56g/L respectively, Conclusion： Curcumin extract have directly killing effect on H1N1 and H3N2 infections.

Development and Characterization of Monoclonal Antibody Specific to Nuclear Protein of Avian Influenza Virus Type A

Five monoclonal antibodies（Mabs） to nuclear protein of avain influenza virus（AIV） were developed by syncretizing SP 2/0 and the spleen cells from BALB of mice immuized with H9 subtype AIV. Specificity of these Mabs were identified by immunofluorescent assay（IFA） and enzyme linked immunosorbent assay （ELISA）. These five Mabs which were named as AIV-NP-2C3, AIV-NP-6A5, AIV-NP-3 H9, AIV-NP-7B4, AIV-NP-2H4 could react with all viruses of AIV-H9 strains in tests. The result of Western blotting showed that only the 60 ku protein antigen of AIV-H9 could be recognized by the Mabs but never recognized by New castle disease virus, REV and infectious bursa disease virus. The result of preliminary application showed that avian influenza viruses could be deetected bv Mabs in IFA and ELISA. All these Mabs will probably play important roles in preventing and monitoring avian influenza viruses.

Designing Primers for H5 and H7 Subtypes of Avian Influenza Virus and Multiplex RT-PCR Amplification

[Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus （AIV） ; [Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank, and design primers（ by Primer Premier 5.0） on high homologous region of these sequences, and then amplified by RT-PCR. [Result] The multiplex RT-PCR amplification, agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV. [Conclusion] It is feasible to rapidly diagnose AIV through this method.

A Review on 2009 Influenza A Virus

[Objective] The paper was to introduce the research progress of 2009 influenza A virus. [Method] 2009 influenza A virus was introduced from the aspects of classification and host, virology, molecular characteristics and vaccine. [Result] A novel influenza A/H1N1 virus emerged in early April 2009 quickly spread worldwide through human-to-human transmission. The virus contained a group of novel gene segments, the nearest known precursor was the virus found in swine. The virus appeared to retain the potential to infect swine again and thus continued reassort with swine viruses. All registered 2009 influenza A vaccines were tested for safety and immunogenicity in clinical trials on human volunteers, and all vaccines were found to be safe, single dose of vaccine could cause protective antibody responses. [Conclusion] The paper provided basis for further study on 2009 influenza A virus.

Complete Genome Sequencing and Genetic Variation Analysis of Two H9N2 Subtype Avian Influenza Virus Strains

[Objective] The study aimed to investigate the genetic variation characters of entire sequences between two H9N2 subtype avian influenza virus strains and other reference strains.[Method] The entire sequences of 8 genes were obtained by using RT-PCR,and these sequences were analyzed with that of six H9N2 subtype avian influenza isolates in homology comparison and genetic evolution relation.[Result] The results showed that the nucleotide sequence of entire gene of the strain shared 91.1%-95.4% homology with other seven reference strains,and PG08 shared the highest homology 91.3% with C/BJ/1/94;ZD06 shared the highest homology 92.3% with D/HK/Y280/97.HA cleavage sites of two H9N2 subtype avian influenza virus isolated strains were PARSSR/GLF,typical of mildly pathogenic avian influenza virus.[Conclusion] Phylogenetic tree for entire gene of eight strains showed that the genetic relationship was the closest between ZD06 and C/Pak/2/99 strains,which belonged to the Eurasian lineage;PG08 shared the highest homology 91.3% with ZD06,it may be the product of gene rearrangements of other sub-lines.

Preparation and Preliminary Identification of Fluorescein Labeled Monoclonal Antibody against Canine Distemper Virus

[Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper （CD） with FITC-conjugated monoclonal antibodies （FITC-McAb）.[ Metbod] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofluorescence assay. [ Result] The absorption test, blocking test and specificity test showed that the labeled antibody had high specificity and sensitivity, but didn＇t have cross reaction with canine parvovirus （CPV）, canine parainfluenza virus （CPIV）, canine adenovirus （CAV） and rabies virus （RV）. The optimal working concentration was 1：80. The positive rate of clinical suspected samples was 48%. [ Conclusion] The direct immunofluorescence assay developed in this study was rapid, specific and convenient, and had great significance for the early diagnosis of canine distemper.

Sequence Comparison and Analysis of HA Gene of Four H9N2 Avian Influenza Virus Isolates

[ Objective] To determine the HA gene sequences of four H9N2 Avian influenza virus （AIV） strains and carry out comparative analysis so as to understand the difference and variation pattern of each strain from the angle of molecular biology and to know the distribution and epidemic law of H9N2 AIV. [Method] One pair of primers was designed referring to HA gene sequences of H9N2 AIV. The HA genes of A/Chicken/Hebei/WD/98 （H9N2; WD98 for short）, A/Chicken/Hebei/ZD/04 （H9N2; ZD04 for short））, A/Chicken/Beijing/MY/06 （H9N2; MY06 for short） ）, and A/Chicken/Beijing/PG/08 （H9N2; PG08 for short）） were amplified, cloned and sequenced. Then the HA gene sequences of these strains were compared with that of 10 H9N2 AIV stains in GenBank. [Result] The ORF of HA genes of the four strains was 1 683 bp in size, encoding 516 amino acids. The HA gene sequences of the four strains, WD98, MY06, PG08, and ZD04, were 82.6% -95.1%, 83.0% -99.0%, 82.7% -95.5%, and 81.3% -95.7% homologous to that of the 10 H9N2 AIV stains, respectively. And the homology of amino acid was respectively 86.6% -96.3%, 86.6% -97.9%, 87.0% -97.1%, and 86.9% -97.3%. [ Conclusion] The HA gene has greatly high homology among different strains.

Effects of spinal cord stimulation on cerebrovascular flow: role of sym-pathetic and parasympathetic innervations

Objective Cervical spinal cord stimulation （SCS） has been found to augment cerebral blood flow （CBF） in a number of animal models. However, the effective use of SCS is hampered by a lack of understanding of its mechanism（s） of action. In this paper, we focus on the sympathetic and parasympathetic effects of SCS on CBF. Method SpragueDawley rats were selected for the experimental series. The animals were divided into 5 groups to underwent SCS and laser Doppler flowmeter （LDF） recordings. Control group, the animal underwent SCS and LDF recordings without any surgery of the nerve fibers and ganglia. V 1 group, the animal underwent bilateral resection of the nasociliary and post-ganglionic parasympathetic nerve fibbers. SCG group, the animal underwent bilateral resection of supper cervical ganglion. V 1 ＋ SCG group, the animal underwent both surgeries as V1- and SCG-group animals did. Sham group, the animal underwent the carotid manipulation with blunt-tipped forceps as well as the dissection of nasociliary and post-ganglionic parasympathetic nerve fibers around the ethmoidal foramen, but without cutting any nerves. Results During the SCS, the LDF was no statistical difference between the V 1 or SCG group and the control group. Yet, the effects of SCS on CBF are completely abolished in V1＋ SCG group. Conclusions Surgical interruption of both the parasympathetic and sympathetic pathways has the contradict effect on SCS-induced CBF augmentation.

Cloning and Phylogenetic Analysis of NS1 Genes from Different Isolates of H9N2 Subtype Duck Influenza Virus

[ Objective] The study aimed to lay a foundation for the further studies on function mechanism of NS1 protein in the interspecies transmission of waterfowl influenza virus. [Method] Using the serologic assay and the specific RT-PCR method, some strains of H9 subtype waterfowl influenza virus were isolated from the 12 to 20 day-old muscovy duck flocks without any clinical symptoms in different areas of Guangdong Province. Four of these strains, including A/duck/ZQ/303/2007（H9N2） （A3 for short）, A/Duck/FJ/301/2007 （H9N2） （C1 for short）, A/Duck/NH/306/2007（H9N2） （ D6 for short）, A/duck/SS/402/2007（H9N2） （ E2 for short）, and a strain named A/duck/ZC/2007（H9N2） （L1 for short） from a muscovy duck died of avian influenza virus （AIV）, were used for NSl gene cloning and sequencing. Subsequently, the obtained NSl gene sequences were compared with other NS1 sequences registered in GenBank, and the phylogenetic analysis was also conducted. [Result] When compared with the H9N2 AIV NS1 sequences in GenBank, the NSl genes of the four AIV strains A3, C1, 136 and E2 displayed homologies ranging from 99% to 100% at nucleotide level, and 95% to 100% at amino acid level; while the NSl gene of L1 strain displayed homology ranging from 94% to 97% at nucleotide level, and 93% to 98% at amino acid level. The phylogenetic tree demonstrated that A3, C1, D6 and E2 were highly resemblant, and L1 was closest to AY66473 （chicken, 2003）. By comparison with the NS1 gene sequences of L1, AF523514 （duck）, AY664743 （chicken） and EF155262.1 （quail） using DNAstar, A3, C1, D6 and E.2 presented nucleotide variations at site 21 （ R→Q）, 70, 71 （ KE→EG）, 86 （ A→S）, 124 （V→M） and 225 （ S→N）, and amino acid variations at site 21,70, 71 and 86 in dsRNA- dependent protein kinase （PKR） binding domain of NSl gene, which induced the evident variations of antigenic determinant and surface proba- bility plot of NS1 protein. [ Conclusion] This study suggested that the amino acid sequence variation in PKR binding domain of NS1 protein had something to do with the virus pathogenicity.

Traffic-aware static channel assignment algorithm in wireless mesh networks

A channel assignment algorithm with awareness of link traffic is proposed in multi-radio multi-channel wireless mesh networks. First, the physical interference model based on the signal-to-interference-plus-noise ratio and successful transmission condition is described. The model is more suitable for a wireless communication environment than other existing models. Secondly, a pure integer quadratic programming （PIQP） model is used to solve the channel assignment problem and improve the capacity of wireless mesh networks. Consequently, a traffic- aware static channel assignment algorithm（TASC） is designed. The algorithm adopts some network parameters, including the network connectivity, the limitation of the number of radios and the successful transmission conditions in wireless communications. The TASC algorithm can diminish network interference and increase the efficiency of channel assignment while keeping the connectivity of the network. Finally, the feasibility and effectivity of the channel assignment solution are illustrated by the simulation results. Compared witb similar algorithms, the proposed algorithm can increase the capacity of WMNs.

MATERIAL SURFACE THERMAL PROPERTY IDENTIFICATION USING HEAT FLUX TACTILE SENSOR

Eased on the mechanism of temperature tactile sensing of human finger,a heat flux tactile sensor com- posed of a thermostat module and a heat flux sensor is designed to identify material thermal properties. The ther- mostat module maintains the sensor temperature invariable, and the heat flux sensor（Peltier device） detects the heat flux temperature difference between the thermostat module and the object surface. Two different modes of the heat flux tactile sensor are proposed, and they are simulated and experimented for different material objects. The results indicate that the heat flux tactile sensor can effectively identify different thermal properties.

Performance Analysis of RF Spiral Inductor with Gradually Changed Metal Width and Space

To decrease the metal losses of RF spiral inductor,a novel layout structure with gradually reduced metal line width and space from outside to inside is presented. This gradual changed inductor has less eddy-current effect than the conventional inductor of fixed metal width and space. So the series resistance can be reduced and the quality （Q） factor of the inductor relating to metal losses is increased. The obtained experimental results corroborate the validity of the proposed method. For a 6nH inductor on high-resistivity silicon at 2.46GHz,Q factor of 14.25 is 11.3% higher than the conventional inductor with the same layout size. This inductor can be integrated with radio frequency integrated circuits to gain better performance in RF front end of a wireless communication system.

Ultrasonic method for measuring water holdup of low velocity and high-water-cut oil-water two-phase flow

Oil reservoirs with low permeability and porosity that are in the middle and late exploitation periods in China＇s onshore oil fields are mostly in the high-water-cut production stage.This stage is associated with severely non-uniform local-velocity flow profiles and dispersed-phase concentration（of oil droplets） in oil-water two-phase flow,which makes it difficult to measure water holdup in oil wells.In this study,we use an ultrasonic method based on a transmission-type sensor in oil-water two-phase flow to measure water holdup in lowvelocity and high water-cut conditions.First,we optimize the excitation frequency of the ultrasonic sensor by calculating the sensitivity of the ultrasonic field using the finite element method for multiphysics coupling.Then we calculate the change trend of sound pressure level attenuation ratio with the increase in oil holdup to verify the feasibility of the employed diameter for the ultrasonic sensor.Based on the results,we then investigate the effects of oildroplet diameter and distribution on the ultrasonic field.To further understand the measurement characteristics of the ultrasonic sensor,we perform a flow loop test on vertical upward oilwater two-phase flow and measure the responses of the optimized ultrasonic sensor.The results show that the ultrasonic sensor yields poor resolution for a dispersed oil slug in water flow（D OS/W flow）,but the resolution is favorable for dispersed oil in water flow（D O/W flow） and very fine dispersed oil in water flow（VFD O/W flow）.This research demonstrates the potential application of a pulsed-transmission ultrasonic method for measuring the fraction of individual components in oil-water two-phase flow with a low mixture velocity and high water cut.

FPGA implementation of bit-stream neuron and perceptron based on sigma delta modulation

To solve the excessive huge scale problem of the traditional multi-bit digital artificial neural network（ANN） hardware implementation methods,a bit-stream ANN hardware implementation method based on sigma delta（ΣΔ） modulation is presented.The bit-stream adder,multiplier,threshold function unit and fully digital ΣΔ modulator are implemented in a field programmable gate array（FPGA）,and these bit-stream arithmetical units are employed to build the bit-stream artificial neuron.The function of the bit-stream artificial neuron is verified through the realization of the logic function and a linear classifier.The bit-stream perceptron based on the bit-stream artificial neuron with the pre-processed structure is proved to have the ability of nonlinear classification.The FPGA resource utilization of the bit-stream artificial neuron shows that the bit-stream ANN hardware implementation method can significantly reduce the demand of the ANN hardware resources.