The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, se...The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.展开更多
[Objective] The aim was to establish the water quality evaluation index system for freshwater aquaculture pond.[Method] The expert survey,DELPHI,field research and other methods were used.Based on the analysis of fact...[Objective] The aim was to establish the water quality evaluation index system for freshwater aquaculture pond.[Method] The expert survey,DELPHI,field research and other methods were used.Based on the analysis of factors influencing water quality of freshwater aquaculture pond,and the importance degree of 14 factors influencing water quality of freshwater aquaculture pond was ordered.Then,five factors were selected as index to establish the water quality evaluation index system of freshwater aquaculture with the determination for thresholds of these factors.[Result] The importance degree of water quality of freshwater aquaculture pond showed an order of Dissolved oxygenpHPhytoplankton biomassSecchi depthTotal NitrogenZooplankton biomassWater temperatureBiochemical Oxygen DemandWater colorSalinityTotal hardness;according to the value of importance degree,the dissolved oxygen,pH,secchi depth,phytoplankton biomass and total nitrogen these five factors were selected as the index system of water quality evaluation of pond.The level of water quality of freshwater aquaculture pond was divided into five,and the tolerance range of fish to each index was obtained by expert observation.[Conclusion] This study had provided a scientific basis for water quality evaluation system of freshwater aquaculture pond.展开更多
In order to explore the effect of Fenlong cultivation in rebuilding and uti- lization of saline-alkali land, the Fenlong tillage machine and Fenlong technology were invented and put into tests in the saline-alkali lan...In order to explore the effect of Fenlong cultivation in rebuilding and uti- lization of saline-alkali land, the Fenlong tillage machine and Fenlong technology were invented and put into tests in the saline-alkali land from 2015-2016 in Xinjiang Autonomous Region and Shaanxi province, respectively. The results showed that in Xinjiang, the total salt content in the heavy saline-alkali soil decreased by 31.31% after one season of cotton planting using Fenlong, while cotton yield increased by 48.80%, and the soil salinity level was fallen from ＂severe＂ to ＂moderate＂ level. In Shaanxi, the total salt content in decreased by 42.73% after planting summer corn summer corn the mild saline-alkali land using Fenlong cultivation, while the corn yield increased by 34.83%, and the soil salinity degree was changed from ＂mild de- salination＂ to ＂normal farmland＂. At last, the paper provided the mechanism and reasons for desalination in soil and yield increase by Fenlong cultivation.展开更多
A dissolved oxygen fuzzy system predicting model based on neural network was put forward in this study. 106 groups of data were used to confirm the fitness of the predicting model. The first 80 groups of data were act...A dissolved oxygen fuzzy system predicting model based on neural network was put forward in this study. 106 groups of data were used to confirm the fitness of the predicting model. The first 80 groups of data were acted as training input and the other 26 groups of data were acted as the confirmed data in the system. The result showed that the testing data was approximately the same as the predicted data. So it gave a new way to solve the problem that the status of the water quality couldn＇t be predicted in time and it＇s hard to watching and measuring the factors dynamic.展开更多
基金Project(50925417)supported by the National Funds for Distinguished Young Scientist,ChinaProject(2012BAC09B04)supported by the National Key Technology Research and Development Program,China+2 种基金Project supported by the Post-doctoral Program of Central South University,ChinaProjects(31100082,61171061)supported by the National Natural Science Foundation of ChinaProject(2012SK4028)supported by the Science and Technology Program of Hunan Province,China
文摘The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.
基金Supported by National Natural Science Foundation of China (40801227)Foundation of Key Laboratory of Aquatic Genetic Resources and Aquacul-tural Ecology,Ministry of Agriculture (KFT2006-7)~~
文摘[Objective] The aim was to establish the water quality evaluation index system for freshwater aquaculture pond.[Method] The expert survey,DELPHI,field research and other methods were used.Based on the analysis of factors influencing water quality of freshwater aquaculture pond,and the importance degree of 14 factors influencing water quality of freshwater aquaculture pond was ordered.Then,five factors were selected as index to establish the water quality evaluation index system of freshwater aquaculture with the determination for thresholds of these factors.[Result] The importance degree of water quality of freshwater aquaculture pond showed an order of Dissolved oxygenpHPhytoplankton biomassSecchi depthTotal NitrogenZooplankton biomassWater temperatureBiochemical Oxygen DemandWater colorSalinityTotal hardness;according to the value of importance degree,the dissolved oxygen,pH,secchi depth,phytoplankton biomass and total nitrogen these five factors were selected as the index system of water quality evaluation of pond.The level of water quality of freshwater aquaculture pond was divided into five,and the tolerance range of fish to each index was obtained by expert observation.[Conclusion] This study had provided a scientific basis for water quality evaluation system of freshwater aquaculture pond.
文摘In order to explore the effect of Fenlong cultivation in rebuilding and uti- lization of saline-alkali land, the Fenlong tillage machine and Fenlong technology were invented and put into tests in the saline-alkali land from 2015-2016 in Xinjiang Autonomous Region and Shaanxi province, respectively. The results showed that in Xinjiang, the total salt content in the heavy saline-alkali soil decreased by 31.31% after one season of cotton planting using Fenlong, while cotton yield increased by 48.80%, and the soil salinity level was fallen from ＂severe＂ to ＂moderate＂ level. In Shaanxi, the total salt content in decreased by 42.73% after planting summer corn summer corn the mild saline-alkali land using Fenlong cultivation, while the corn yield increased by 34.83%, and the soil salinity degree was changed from ＂mild de- salination＂ to ＂normal farmland＂. At last, the paper provided the mechanism and reasons for desalination in soil and yield increase by Fenlong cultivation.
基金Supported by National Natural Science Foundation of China (40801227)Open Foundation of Marine and Estuarine Fisheries Resources of Ministry of Agriculture and the Key Laboratory of Ecology (Open-2-04-09)~~
文摘A dissolved oxygen fuzzy system predicting model based on neural network was put forward in this study. 106 groups of data were used to confirm the fitness of the predicting model. The first 80 groups of data were acted as training input and the other 26 groups of data were acted as the confirmed data in the system. The result showed that the testing data was approximately the same as the predicted data. So it gave a new way to solve the problem that the status of the water quality couldn＇t be predicted in time and it＇s hard to watching and measuring the factors dynamic.
