In order to investigate the effects of cadmium on the immune defense function of spiders infected with bacteria,Pirata subpiraticus was taken as research object,and the cadmium-treated group and the control group were...In order to investigate the effects of cadmium on the immune defense function of spiders infected with bacteria,Pirata subpiraticus was taken as research object,and the cadmium-treated group and the control group were all infected with bacteria(S.aureus and E.coli).After 6 and 24 h of infection,the energy substances contents and the activity of immune detoxification enzyme system in Pirata subpiraticus were determined.The results showed that after 6 and 24 h of infection with S.aureus and 6 h of infection with E.coli,the amount of soluble sugars and soluble proteins of Pirata subpiraticus in the cadmium-treated group increased significantly.Compared with the control,after 6 h of infection with S.aureus and 24 h of infection with E.coli,the activity of acetylcholinesterase(AChE)of Pirata subpiraticus in the cadmium-treated group decreased significantly.This indicated that cadmium inhibited the activity of AChE in infected Pirata subpiraticus.After 24 h of infection with S.aureus and 6 h of infection with E.coli,the activity of polyphenol oxidase(PPO)was signicantly higher than that of the control,indicating that cadmium induced the activity of PPO in infected Pirata subpiraticus.These results suggested that cadmium can affect the content of energy substance and immune detoxification enzyme activity in infected Pirata subpiraticus.展开更多
文摘In order to investigate the effects of cadmium on the immune defense function of spiders infected with bacteria,Pirata subpiraticus was taken as research object,and the cadmium-treated group and the control group were all infected with bacteria(S.aureus and E.coli).After 6 and 24 h of infection,the energy substances contents and the activity of immune detoxification enzyme system in Pirata subpiraticus were determined.The results showed that after 6 and 24 h of infection with S.aureus and 6 h of infection with E.coli,the amount of soluble sugars and soluble proteins of Pirata subpiraticus in the cadmium-treated group increased significantly.Compared with the control,after 6 h of infection with S.aureus and 24 h of infection with E.coli,the activity of acetylcholinesterase(AChE)of Pirata subpiraticus in the cadmium-treated group decreased significantly.This indicated that cadmium inhibited the activity of AChE in infected Pirata subpiraticus.After 24 h of infection with S.aureus and 6 h of infection with E.coli,the activity of polyphenol oxidase(PPO)was signicantly higher than that of the control,indicating that cadmium induced the activity of PPO in infected Pirata subpiraticus.These results suggested that cadmium can affect the content of energy substance and immune detoxification enzyme activity in infected Pirata subpiraticus.
