电影《神迹》（原名Something The Lord Made）也翻译为《上帝造物》、《天赐良医》、《上帝的杰作》,是一部时常110分钟的英语传记片,同时也是一部关于医疗试验的影片.影片根据真实故事改编,记录了白人医生阿尔佛雷德·布雷拉克（...电影《神迹》（原名Something The Lord Made）也翻译为《上帝造物》、《天赐良医》、《上帝的杰作》,是一部时常110分钟的英语传记片,同时也是一部关于医疗试验的影片.影片根据真实故事改编,记录了白人医生阿尔佛雷德·布雷拉克（阿伦·瑞克曼Alan Rickman饰）与黑人合作伙伴维文·托马斯（茅斯·达夫Mos Def饰）攻克法洛四联症（又称紫绀婴儿）的重大医学事件.展开更多
Anti β NGF antiserum was prepared by immunizing rabbits with purified mouse β NGF. It presented immunopositivity to β NGF on Western blot, resulting in a single band approximately at the molecular weight of 14 kD...Anti β NGF antiserum was prepared by immunizing rabbits with purified mouse β NGF. It presented immunopositivity to β NGF on Western blot, resulting in a single band approximately at the molecular weight of 14 kDa. The antiserum was further purified into anti β NGF IgG by affinity chromatography. Chicken embryonic dorsal root ganglia (DRG) were cultured to test the biological activities of β NGF and its antibody.展开更多
This paper addresses the problem of three-dimensional trajectory tracking control for underactuated autonomous underwater vehicles in the presence of parametric uncertainties,environmental disturbances and input satur...This paper addresses the problem of three-dimensional trajectory tracking control for underactuated autonomous underwater vehicles in the presence of parametric uncertainties,environmental disturbances and input saturation.First,a virtual guidance control strategy is established on the basis of tracking error kinematics,which resolves the overall control system into two cascade subsystems.Then,a first-order sliding mode differentiator is introduced in the derivation to avoid tedious analytic calculation,and a Gaussian error function-based continuous differentiable symmetric saturation model is explored to tackle the issue of input saturation.Combined with backstepping design techniques,the neural network control method and an adaptive control approach are used to estimate composite items of the unknown uncertainty and approximation errors.Meanwhile,Lyapunov-based stability analysis guarantees that control error signals of the closed-loop system are uniformly ultimately bounded.Finally,simulation studies are conducted for the trajectory tracking of a moving target and a spiral line to validate the effectiveness of the proposed controller.展开更多
Human cytomegalovirus （HCMV） is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of...Human cytomegalovirus （HCMV） is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia ceils by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.展开更多
A dynamic velocity feed-forward compensation （RBF-NN） dynamic model identification was presented for control （DVFCC） approach with RBF neural network the adaptive trajectory tracking of industrial robots. The prop...A dynamic velocity feed-forward compensation （RBF-NN） dynamic model identification was presented for control （DVFCC） approach with RBF neural network the adaptive trajectory tracking of industrial robots. The proposed control approach combined the advantages of traditional feedback closed-loop position control and computed torque control based on inverse dynamic model. The feed-forward compensator used a nominal robot dynamics as accurate dynamic model and on-line identification with RBF-NN as uncertain part to improve dynamic modeling accu- racy. The proposed compensation was applied as velocity feed-forward by an inverse velocity controller that can con- vert torque signal into velocity in the standard industrial controller. Then, the need for a torque control interface was avoided in the real-time dynamic control of industrial robot. The simulations and experiments were carried out on a gas cutting manipulator. The results show that the proposed control approach can reduce steady-state error, suppress overshoot and enhance tracking accuracy and efficiency in joint space and Cartesian space, especially under high- speed condition.展开更多
Fermentative production of chlortetracycline is a complex fed-batch bioprocess. It generally takes over 90 h for cultivation and is often contaminated by undesired microorganisms. Once the fermentation system is conta...Fermentative production of chlortetracycline is a complex fed-batch bioprocess. It generally takes over 90 h for cultivation and is often contaminated by undesired microorganisms. Once the fermentation system is contaminated to certain extent, the product quality and yield will be seriously affected, leading to a substantial economic loss. Using information fusion based on the Dezer–Smarandache theory, self-recursive wavelet neural network and unscented kalman filter, a novel method for online prediction of contamination is developed. All state variables of culture process involving easy-to-measure and difficult-to-measure variables commonly obtained with soft-sensors present their contamination symptoms. By extracting and fusing latent information from the changing trend of each variable, integral and accurate prediction results for contamination can be achieved. This makes preventive and corrective measures be taken promptly. The field experimental results show that the method can be used to detect the contamination in time, reducing production loss and enhancing economic efficiency.展开更多
Surgical accesses to lesions of the posterolateral pontomesencephalic junction（PMJ）region and the posterolateral tentorial gap remain a challenge in the field of neurosurgery.Since the first report of application of...Surgical accesses to lesions of the posterolateral pontomesencephalic junction（PMJ）region and the posterolateral tentorial gap remain a challenge in the field of neurosurgery.Since the first report of application of the extreme lateral supracerebellar infratentorial（ELSI）approach in resecting the PMJ lesions in 2000,a few articles concerning the ELSI approach have been published.The present review mainly provided an intimate introduction of the ELSI approach,and evalu- ated it in facets of patient position,skin incision,craniectomy,draining veins,retraction against the cerebellum,exposure limits,patient healing,as well as advantages and limitations compared with other approaches.The ELSI approach is proposed to be a very young and promising approach to access the lesions of posterolateral PMJ region and the posterolateral tentorial gap.Besides,it has several advantages such as having a shorter surgical pathway,causing less surgical complications,laborsaving,etc.Still,more studies are needed to improve this approach.展开更多
Multivariate statistical analyses were used to assess the contents and distributions of trace elements in agricultural soils in Xinzhou of Shanxi Province, China, and to identify their sources. Samples with high level...Multivariate statistical analyses were used to assess the contents and distributions of trace elements in agricultural soils in Xinzhou of Shanxi Province, China, and to identify their sources. Samples with high levels of trace elements were concentrated in eastern Xinzhou, with contents declining from the east to west. Principal component and redundancy analyses revealed strong correlations among Co, Cu, Mn, Ni, Se, V, and Zn contents, suggesting that these elements were derived from similar parent materials. There were also strong correlations between the contents of these elements and soil properties. Contents of Cd and Pb were significantly higher in the agricultural soil samples than in the background soil samples(P < 0.05), and were higher in areas with higher levels of gross domestic product but decreased with distance to the nearest road. Therefore, human activities appear to have a strong influence on the Cd and Pb distribution patterns. A novel artificial neural network(ANN) model, using environmental input data, was used to predict the soil Cd and Pb contents of specified test dates. The performances of the ANN model and a traditional multilinear model were compared. The ANN model could successfully predict Cd and Pb content distributions, projecting that soil Cd and Pb contents will increase by 128% and 25%, respectively, by 2020. The results thus indicated that the economic condition of an area has a greater effect on trace element contents and distributions in the soil than the scale of the economy itself.展开更多
The inhibitory co-receptor programmed death 1 （PD-1, encoded by pdcdl） and its two ligands PD-L1 and PD-L2 comprise an important immune inhibitory signaling pathway for defense against microbes and for self-toleranc...The inhibitory co-receptor programmed death 1 （PD-1, encoded by pdcdl） and its two ligands PD-L1 and PD-L2 comprise an important immune inhibitory signaling pathway for defense against microbes and for self-tolerance. Unlike other members of the B7-CD28 family, expression of PD-L1 and PD-L2 is not limited to the immune system. In this study, we determined that a polyclonal antibody （pAb） （R＆D Systems） against extracellular domains of mouse PD-L2 （mPD-L2） could recognize anti- gen（s） in diverse mouse tissues, including the anterior and intermediate pituitary gland, olfactory bulbs and olfactory epitheli- um, tongue epithelium, keratinized epithelial ceils and skin and whisker hair follicles. These findings differed from previous reports of mPD-L2 localization. Reverse transcription PCR and Western blot analyses, however, were unable to detect any mPD-L2 transcripts or proteins of the 25-kD predicted molecular weight in RNA and protein extracts, respectively, from the above tissues, suggesting that the anti-mPD-L2 pAb cross-reacts with certain novel antigen（s）. Developmental studies revealed that the earliest expression of mPD-L2-1ike antigen was in the olfactory epithelium at embryonic day 12.5 （E12.5）. At E14.5, mPD-L2-1ike antigen was present in the skin, tongue and follicles of the skin and whiskers. The distribution patterns of mPD-L2-1ike antigen remained similar from E18.5 to adulthood. The results of bioinformatic analysis and other experiments suggested neural cell adhesion molecule and hemicentin-1 as candidate proteins with cross-reactivity to the anti-mPD-L2 pAb. These results demonstrate that care is required in interpreting staining patterns generated when anti-PD-L2 pAb is used to lo-cate PD-L2-expressing cells in the central nervous system and epithelial tissues, such as the olfactory epithelium. In addition, this anti-PD-L2 pAb may be used as an alternative antibody for labeling the olfactory epithelium during embryonic develop-ment in mice.展开更多
Trace theory is a very important linguistic hypothesis within the framework of Generative Grammar. But whether the theory is correct, namely whether the assumed empty trace exists in syntactic representation, must be ...Trace theory is a very important linguistic hypothesis within the framework of Generative Grammar. But whether the theory is correct, namely whether the assumed empty trace exists in syntactic representation, must be verified by experiments concerning the neural mechanisms of language. On the basis of Generative Grammar, the current study uses event- related potentials （ERP） to examine the neural mechanism of trace in Chinese topicalized constructions. The results show that three types of waves （a sustained negativity, a negativity elicited by the verb and a P600 elicited at the sentence-final position） are observed in the processing of Chinese topicalized constructions. These neural electrophysiological indexes indicate that the sentence-initial topic does leave a trace in the position out of which it moves, i.e. the sentence-final position, suggesting that there is a syntactic dependency between the topic and its trace. Consequently, we draw the conclusion that trace is neurologically supported in Chinese topicalized constructions and trace theory isneurophysiologically viable. Meanwhile, the study also adds neural evidence to the view that Chinese topicalized constructions are structures derived from syntactic movement.展开更多
During free exploration,the emergence of patterned and sequential behavioral responses to an unknown environment reflects exploration traits and adaptation.However,the behavioral dynamics and neural substrates underly...During free exploration,the emergence of patterned and sequential behavioral responses to an unknown environment reflects exploration traits and adaptation.However,the behavioral dynamics and neural substrates underlying the exploratory behavior remain poorly understood.We developed computational tools to quantify the exploratory behavior and performed in vivo electrophysiological recordings in a large arena in which mice made sequential excursions into unknown territory.Occupancy entropy was calculated to characterize the cumulative and moment-to-moment behavioral dynamics in explored and unexplored territories.Local field potential analysis revealed that the theta activity in the dorsal hippocampus(d HPC)was highly correlated with the occupancy entropy.Individual d HPC and prefrontal cortex(PFC)oscillatory activities could classify various aspects of free exploration.Initiation of exploration was accompanied by a coordinated decrease and increase in theta activity in PFC and d HPC,respectively.Our results indicate that d HPC and PFC work synergistically in shaping free exploration by modulating exploratory traits during emergence and visits to an unknown environment.展开更多
文摘电影《神迹》（原名Something The Lord Made）也翻译为《上帝造物》、《天赐良医》、《上帝的杰作》,是一部时常110分钟的英语传记片,同时也是一部关于医疗试验的影片.影片根据真实故事改编,记录了白人医生阿尔佛雷德·布雷拉克（阿伦·瑞克曼Alan Rickman饰）与黑人合作伙伴维文·托马斯（茅斯·达夫Mos Def饰）攻克法洛四联症（又称紫绀婴儿）的重大医学事件.
文摘Anti β NGF antiserum was prepared by immunizing rabbits with purified mouse β NGF. It presented immunopositivity to β NGF on Western blot, resulting in a single band approximately at the molecular weight of 14 kDa. The antiserum was further purified into anti β NGF IgG by affinity chromatography. Chicken embryonic dorsal root ganglia (DRG) were cultured to test the biological activities of β NGF and its antibody.
基金Project(51979116)supported by the National Natural Science Foundation of ChinaProject(2018KFYYXJJ012,2018JYCXJJ045)supported by the Fundamental Research Funds for the Central Universities,China+1 种基金Project(YT19201702)supported by the Innovation Foundation of Maritime Defense Technologies Innovation Center,ChinaProject supported by the HUST Interdisciplinary Innovation Team Project,China。
文摘This paper addresses the problem of three-dimensional trajectory tracking control for underactuated autonomous underwater vehicles in the presence of parametric uncertainties,environmental disturbances and input saturation.First,a virtual guidance control strategy is established on the basis of tracking error kinematics,which resolves the overall control system into two cascade subsystems.Then,a first-order sliding mode differentiator is introduced in the derivation to avoid tedious analytic calculation,and a Gaussian error function-based continuous differentiable symmetric saturation model is explored to tackle the issue of input saturation.Combined with backstepping design techniques,the neural network control method and an adaptive control approach are used to estimate composite items of the unknown uncertainty and approximation errors.Meanwhile,Lyapunov-based stability analysis guarantees that control error signals of the closed-loop system are uniformly ultimately bounded.Finally,simulation studies are conducted for the trajectory tracking of a moving target and a spiral line to validate the effectiveness of the proposed controller.
基金National Natural Science Foundation of China (30770105)Mt. Tai Scholar Construction Engineering Special Foundation of Shandong province.
文摘Human cytomegalovirus （HCMV） is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia ceils by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.
文摘A dynamic velocity feed-forward compensation （RBF-NN） dynamic model identification was presented for control （DVFCC） approach with RBF neural network the adaptive trajectory tracking of industrial robots. The proposed control approach combined the advantages of traditional feedback closed-loop position control and computed torque control based on inverse dynamic model. The feed-forward compensator used a nominal robot dynamics as accurate dynamic model and on-line identification with RBF-NN as uncertain part to improve dynamic modeling accu- racy. The proposed compensation was applied as velocity feed-forward by an inverse velocity controller that can con- vert torque signal into velocity in the standard industrial controller. Then, the need for a torque control interface was avoided in the real-time dynamic control of industrial robot. The simulations and experiments were carried out on a gas cutting manipulator. The results show that the proposed control approach can reduce steady-state error, suppress overshoot and enhance tracking accuracy and efficiency in joint space and Cartesian space, especially under high- speed condition.
文摘Fermentative production of chlortetracycline is a complex fed-batch bioprocess. It generally takes over 90 h for cultivation and is often contaminated by undesired microorganisms. Once the fermentation system is contaminated to certain extent, the product quality and yield will be seriously affected, leading to a substantial economic loss. Using information fusion based on the Dezer–Smarandache theory, self-recursive wavelet neural network and unscented kalman filter, a novel method for online prediction of contamination is developed. All state variables of culture process involving easy-to-measure and difficult-to-measure variables commonly obtained with soft-sensors present their contamination symptoms. By extracting and fusing latent information from the changing trend of each variable, integral and accurate prediction results for contamination can be achieved. This makes preventive and corrective measures be taken promptly. The field experimental results show that the method can be used to detect the contamination in time, reducing production loss and enhancing economic efficiency.
基金supported by the grants from Natural Science Foundation of Shandong Province(No.Z2008C12,Y2006C13)
文摘Surgical accesses to lesions of the posterolateral pontomesencephalic junction（PMJ）region and the posterolateral tentorial gap remain a challenge in the field of neurosurgery.Since the first report of application of the extreme lateral supracerebellar infratentorial（ELSI）approach in resecting the PMJ lesions in 2000,a few articles concerning the ELSI approach have been published.The present review mainly provided an intimate introduction of the ELSI approach,and evalu- ated it in facets of patient position,skin incision,craniectomy,draining veins,retraction against the cerebellum,exposure limits,patient healing,as well as advantages and limitations compared with other approaches.The ELSI approach is proposed to be a very young and promising approach to access the lesions of posterolateral PMJ region and the posterolateral tentorial gap.Besides,it has several advantages such as having a shorter surgical pathway,causing less surgical complications,laborsaving,etc.Still,more studies are needed to improve this approach.
基金supported by the National High Technology Research and Development Program of China (863 Program) (Nos. 2012AA100601 and 2012AA101401)the National Natural Science Foundation of China (Nos. 41271338 and 41301342)
文摘Multivariate statistical analyses were used to assess the contents and distributions of trace elements in agricultural soils in Xinzhou of Shanxi Province, China, and to identify their sources. Samples with high levels of trace elements were concentrated in eastern Xinzhou, with contents declining from the east to west. Principal component and redundancy analyses revealed strong correlations among Co, Cu, Mn, Ni, Se, V, and Zn contents, suggesting that these elements were derived from similar parent materials. There were also strong correlations between the contents of these elements and soil properties. Contents of Cd and Pb were significantly higher in the agricultural soil samples than in the background soil samples(P < 0.05), and were higher in areas with higher levels of gross domestic product but decreased with distance to the nearest road. Therefore, human activities appear to have a strong influence on the Cd and Pb distribution patterns. A novel artificial neural network(ANN) model, using environmental input data, was used to predict the soil Cd and Pb contents of specified test dates. The performances of the ANN model and a traditional multilinear model were compared. The ANN model could successfully predict Cd and Pb content distributions, projecting that soil Cd and Pb contents will increase by 128% and 25%, respectively, by 2020. The results thus indicated that the economic condition of an area has a greater effect on trace element contents and distributions in the soil than the scale of the economy itself.
基金supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China (Grant No. HG3102)National Natural Science Foundation of China (Grant No. 81100899)
文摘The inhibitory co-receptor programmed death 1 （PD-1, encoded by pdcdl） and its two ligands PD-L1 and PD-L2 comprise an important immune inhibitory signaling pathway for defense against microbes and for self-tolerance. Unlike other members of the B7-CD28 family, expression of PD-L1 and PD-L2 is not limited to the immune system. In this study, we determined that a polyclonal antibody （pAb） （R＆D Systems） against extracellular domains of mouse PD-L2 （mPD-L2） could recognize anti- gen（s） in diverse mouse tissues, including the anterior and intermediate pituitary gland, olfactory bulbs and olfactory epitheli- um, tongue epithelium, keratinized epithelial ceils and skin and whisker hair follicles. These findings differed from previous reports of mPD-L2 localization. Reverse transcription PCR and Western blot analyses, however, were unable to detect any mPD-L2 transcripts or proteins of the 25-kD predicted molecular weight in RNA and protein extracts, respectively, from the above tissues, suggesting that the anti-mPD-L2 pAb cross-reacts with certain novel antigen（s）. Developmental studies revealed that the earliest expression of mPD-L2-1ike antigen was in the olfactory epithelium at embryonic day 12.5 （E12.5）. At E14.5, mPD-L2-1ike antigen was present in the skin, tongue and follicles of the skin and whiskers. The distribution patterns of mPD-L2-1ike antigen remained similar from E18.5 to adulthood. The results of bioinformatic analysis and other experiments suggested neural cell adhesion molecule and hemicentin-1 as candidate proteins with cross-reactivity to the anti-mPD-L2 pAb. These results demonstrate that care is required in interpreting staining patterns generated when anti-PD-L2 pAb is used to lo-cate PD-L2-expressing cells in the central nervous system and epithelial tissues, such as the olfactory epithelium. In addition, this anti-PD-L2 pAb may be used as an alternative antibody for labeling the olfactory epithelium during embryonic develop-ment in mice.
基金supported by grants from the Major Program of the National Social Science Fund of China(10&ZD126)the National Natural Science Foundation of China(31271196)+5 种基金the Humanities and Social Science Project of the Ministry of Education(13YJC740057)the Social Science Foundation of Jiangsu Province(11YYC011)the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Major Program of Philosophy and Social Science Key Research Base of Jiangsu Higher Education Institutionsthe Major Basic Research Program of the Natural Science Foundation of Jiangsu Higher Education Institutions(10KJA180051)the Faculty Research Grant of Jiangsu Normal University(11XLR47)
文摘Trace theory is a very important linguistic hypothesis within the framework of Generative Grammar. But whether the theory is correct, namely whether the assumed empty trace exists in syntactic representation, must be verified by experiments concerning the neural mechanisms of language. On the basis of Generative Grammar, the current study uses event- related potentials （ERP） to examine the neural mechanism of trace in Chinese topicalized constructions. The results show that three types of waves （a sustained negativity, a negativity elicited by the verb and a P600 elicited at the sentence-final position） are observed in the processing of Chinese topicalized constructions. These neural electrophysiological indexes indicate that the sentence-initial topic does leave a trace in the position out of which it moves, i.e. the sentence-final position, suggesting that there is a syntactic dependency between the topic and its trace. Consequently, we draw the conclusion that trace is neurologically supported in Chinese topicalized constructions and trace theory isneurophysiologically viable. Meanwhile, the study also adds neural evidence to the view that Chinese topicalized constructions are structures derived from syntactic movement.
基金the National Natural Science Foundation of China(32070985)Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions(NYKFKT20190018)Shenzhen Key Laboratory of Translational Research for Brain Diseases(ZDSYS20200828154800001)。
文摘During free exploration,the emergence of patterned and sequential behavioral responses to an unknown environment reflects exploration traits and adaptation.However,the behavioral dynamics and neural substrates underlying the exploratory behavior remain poorly understood.We developed computational tools to quantify the exploratory behavior and performed in vivo electrophysiological recordings in a large arena in which mice made sequential excursions into unknown territory.Occupancy entropy was calculated to characterize the cumulative and moment-to-moment behavioral dynamics in explored and unexplored territories.Local field potential analysis revealed that the theta activity in the dorsal hippocampus(d HPC)was highly correlated with the occupancy entropy.Individual d HPC and prefrontal cortex(PFC)oscillatory activities could classify various aspects of free exploration.Initiation of exploration was accompanied by a coordinated decrease and increase in theta activity in PFC and d HPC,respectively.Our results indicate that d HPC and PFC work synergistically in shaping free exploration by modulating exploratory traits during emergence and visits to an unknown environment.
