Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupunctur...Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupuncturing growing point,respectively.The doubling rate and mortality rate in different treatments were analyzed by variance analysis and multiple comparisons.The result showed that growing point acupuncturing method exhibited the highest doubling efficiency with an average doubling rate of 23%,seed-setting rate of 21.4%,and mortality rate of 16.3%.Composed with other two chemical doubling methods,growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower application dose of colchicine.This study laid the foundation for industrial application of haploid breeding techniques.展开更多
With apical leaves of Lycium ruthenicum Murr as experimental material, the polyploids of L. ruthenicum were induced with colchicine solution, and total four polyploidy plants were identified by chromosome courts. The ...With apical leaves of Lycium ruthenicum Murr as experimental material, the polyploids of L. ruthenicum were induced with colchicine solution, and total four polyploidy plants were identified by chromosome courts. The results showed that tetraploid plants of L. ruthenicum were successfully induced with 300 mg/L of colchicine solution after 7 and 21 d of induction, with 400 mg/L of colchicine solution after 14 days of induction, and with 500 mg/L of colchicine solution after 7 days of induction, respectively.展开更多
[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with dif...[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.展开更多
文摘Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupuncturing growing point,respectively.The doubling rate and mortality rate in different treatments were analyzed by variance analysis and multiple comparisons.The result showed that growing point acupuncturing method exhibited the highest doubling efficiency with an average doubling rate of 23%,seed-setting rate of 21.4%,and mortality rate of 16.3%.Composed with other two chemical doubling methods,growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower application dose of colchicine.This study laid the foundation for industrial application of haploid breeding techniques.
基金Supported by 2016 College Students’Innovative Entrepreneurial Training Program of Yanbian University(ydbksky2016127)~~
文摘With apical leaves of Lycium ruthenicum Murr as experimental material, the polyploids of L. ruthenicum were induced with colchicine solution, and total four polyploidy plants were identified by chromosome courts. The results showed that tetraploid plants of L. ruthenicum were successfully induced with 300 mg/L of colchicine solution after 7 and 21 d of induction, with 400 mg/L of colchicine solution after 14 days of induction, and with 500 mg/L of colchicine solution after 7 days of induction, respectively.
基金Supported by Agricultural Seed Breeding Program of Shandong Province in 2014([2014]96)
文摘[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.
