The dynamic concentrations of glucose, fructose, sucrose and total sugar were determined through exogenous ABA and GA3 treatments during young period of fruit and before fruit coloring in pulp of ‘Cara cara’ navel o...The dynamic concentrations of glucose, fructose, sucrose and total sugar were determined through exogenous ABA and GA3 treatments during young period of fruit and before fruit coloring in pulp of ‘Cara cara’ navel orange. The results are as fol ows: 10 mg/L ABA increased glucose, fructose and total sugar concen-tration significantly or very significantly, ABA treatment of 50 mg/L increased sucrose concentration very significantly, but ABA treatment of 100 mg/L decreased glucose concentration very significantly. GA3 treatment of lower and middle concentrations(10, 50 and 250 mg/L) increased sucrose concentration very significantly, GA3 treatment of 10 mg/L had no remarkable effect on glucose and fructose concentration but in-creased total sugar concentration very significantly, GA3 treatment of 50, 250 and 500 mg/L decreased glucose, fructose and total sugar concentration very significant-ly. Therefore, ABA treatment of lower concentration could increase one or several kinds of sugar concentration, but GA3 treatment of higher concentration (250 and 500 mg/L) restrained sugar concentration in pulp of ‘Cara cara’ navel orange seri-ously.展开更多
A full-length cDNA encoding fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from maize (Zea mays L.) was cloned by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplifica...A full-length cDNA encoding fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from maize (Zea mays L.) was cloned by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and designated as mF2KP. The encoded protein is composed of two regions. Its COOH-terminal region is catalytic region and homologous to the enzymes from other eukaryotes; and its NH 2-terminal region is common and special region only in plant. A truncated fragment of mF2KP covering integrated catalytic region was expressed in Escherichia coli. The fusion protein had the activities of fructose-6-phosphate, 2-kinase as well as fructose-2,6-bisphosphatase. Northern blot showed that the transcript level of mF2KP in seedlings initiated from strong-vigor seeds is lower than that from weak-vigor seeds.展开更多
[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6' kiwifruit (Actinidia eriantha Benth) were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as mater...[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6' kiwifruit (Actinidia eriantha Benth) were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as materials every 15 days since 20 days after full bloom (DAFB) to 170 DAFB until in mature stage. [Result] The results showed that during fruit development of ‘Ganmi 6', the total sugar soluble contents had a rising trend with relatively stable at 95 DAFB, then rose until har- vest with the maximum content (10.35%). The titratable acid content showed a trend of increasing, then declining, then increase to the harvest content (1.10%). From the sugar acid ratio, we can knew it decreased in 95 DAFB, then up to the max (9.38). The changes of AsA contents showed double ‘S' shape, decreased af- ter the first increased rapidly, slightly increased and then decreased in the early harvest. [Conclusion] It provided a theoretical basis for scientific cultivation methods to explore the nutrients regulation.展开更多
[ Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus. [ Method ] The free radical scavenging ab...[ Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus. [ Method ] The free radical scavenging ability and reducing capacity of DPPH as well as inhibitory rate of nonenzymatic glycation reaction were measured targets for comparing changes of anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of fermentation lyophilizer and fermentation extraction before and after EGB fermention adsorbed by AB-8 macroporous resin. The EGB fermention was biotransformed by Hericium erinaceus. [ Result ] After adsorbed by AB-8 macroporous resin, the bioactive matters were concentrated and separated. The free radical scavenging rate, reducing capacity and inhibitory rate of nonenzymatic glycation reaction were increased significantly after adsorbed by AB-8 macroporous resin. [ Conclusion] AB-8 macroporous resin could be used for preliminary concentration of EGB fermentation which was biotransformed by Hericium erinaceus.展开更多
Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Bat...Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Batsch cv. Feicheng) is described. The pMT18 clone is 1188 bp in length, with an open reading frame of 393 amino acids. The homology and phylogenetic analyses indicate a remarkable similarity between peach PG and other ripening related PG. And seven consensus sequences have revealed in peach PG compared to the PG from other plants. However, the profound divergence with other PG and the unique structure features suggest that peach PG probably belongs to a new evolutionary class. In RT PCR analysis, pMT18 related RNA was undetectable in leaves, and was much abundant in ripe fruits. The ripening specific expression pattern of this cDNA will be useful in investigating the roles of PG in fruit ripening and developing a transgenic peach with the improved post harvesting quality in the future.展开更多
The variation of sugar, acid and vitamin C contents in fruits of red flesh kiwifruit (Actinidia chinensis) "Hongyang" and green fresh kiwifruit (A. deliciosa) 'Jinkui' were investigated during fruit developmen...The variation of sugar, acid and vitamin C contents in fruits of red flesh kiwifruit (Actinidia chinensis) "Hongyang" and green fresh kiwifruit (A. deliciosa) 'Jinkui' were investigated during fruit development. The results showed that the to- tal sugar soluble contents of "Hongyang' and 'Jinkui" during fruit development ex- isted different variations. With the upward trend of 'Hongyang' fruit development, 95 days after full bloom (DAFB), the total soluble sugar accumulation was relatively slow, and then rose rapidly until harvest with the maximum content (6.87%). While 'Jinkui' fruit showed a fluctuant process, rising in 50 DAFB, then declining, then rising rapidly and decreasing slightly right before harvest. The variation of fruit titrat- able acid between them was more consistent, which was increased and then de- creased. The only difference was that the titratable acid content of 'Jinkui' fruit de- creased slowly from the late of fruit development to fruit ripening, similar to the maximum value, but that of 'Hongyang' fruit decreased rapidly in the late. Titrat- able acid contents of them in the maturity were 1.08% and 1.20%, respectively. The trends of sugar acid ratio for 'Hongyang' and 'Jinkui' fruit were quite different. 'Hongyang' fruit increased slowly and then rapidly; 'Jinkui' changed from decreas- ing to increasing, followed by slight decreasing in mature stage. In addition, the two kiwifruit varieties had a similar change trend of Vc content during fruit development, which changed from rapid increasing to declining, followed by slight growing in the harvest. The changing tendency of 'Jinkui' fruit was later than that of 'Hengyang'.展开更多
[Objective] The aim was to study the preservation of fresh Chinese wolfberry fruit via chitosan coating. [Method] Using Chinese wolfberry "Ningqi No. 1" as experimental material, the fresh fruits were coated...[Objective] The aim was to study the preservation of fresh Chinese wolfberry fruit via chitosan coating. [Method] Using Chinese wolfberry "Ningqi No. 1" as experimental material, the fresh fruits were coated by different concentrations of chitosan(0.75%, 1.00%, 1.25%, 1.50% and 1.75%) at room temperature to study the changes in weight loss rate, rate of rotten fruits rot, soluble solid content and vitamin C(Vc) content. [Result] Of all the treatments, the fruits coated with 1.25% chitosan showed lowest rate of rotten fruit and weight loss rate, and highest Vc and soluble solid content, presenting the optimal fresh-keeping effect. [Conclusion] Chitosan coating is helpful for the preservation of fresh Chinese wolfberry fruit at room temperature.展开更多
A natural attapulgite (ATP)‐based catalyst, sulfated In2O3‐ATP (SO42-/In2O3‐ATP), was obtained by an impregnation‐calcination method and was used to efficiently and selectively produce the useful platform chem...A natural attapulgite (ATP)‐based catalyst, sulfated In2O3‐ATP (SO42-/In2O3‐ATP), was obtained by an impregnation‐calcination method and was used to efficiently and selectively produce the useful platform chemical 5‐hydroxymethylfurfural (HMF) from hexoses. Some important reaction param‐eters were studied, revealing that Lewis and Br-nsted acid sites on SO42-/In2O3‐ATP catalyze glu‐cose isomerization and fructose dehydration. The yields of HMF from glucose and fructose were 40.2%and 46.2%, respectively, using the optimal conditions of 180℃ for 60 min with 10 wt%of solid acid catalyst in a mixture of γ‐valerolactone‐water (9:1).展开更多
Objective: To study the effects of sodium magnesiusm fructose diphosphate(FDPM) on brain damage of rats after ischemia-reperfusion. Methods: Rats were subjected to cerebral ischemia-reperfusion induced by inserting a ...Objective: To study the effects of sodium magnesiusm fructose diphosphate(FDPM) on brain damage of rats after ischemia-reperfusion. Methods: Rats were subjected to cerebral ischemia-reperfusion induced by inserting a nylon thread into internal carotid artery to block the origin of middle cerebral artery and removing the thread later. FDPM (400 mg·kg -1), fructose-1,6-diphosphate (FDP, 400 mg·kg -1)and magnesium sulfate (MgSO 4, 30 mg·kg -1) were administrated 10 min after the onset of ischemia. Neurological scale, brain infarct area, Malondialdehyde(MDA) content and histopathological changes of brain tissue were studied. Results: FDPM decreased neurological scale, diminished brain infarct area, reduced MDA content and relieved histopathological change of rat brain tissue subjected to ischemia-reperfusion. These effects were more powerful than that of FDP or MgSO 4. Conclusions: It is suggested that FDPM markedly prevented rats against brain damage after cerebral ischemia-reperfusion, and its effect was better than that of FDP or MgSO 4.展开更多
The 1 195 bp 5′ flanking region of rice ( Oryza sativa L.) cytosolic fructose_1, 6_bisphosphatase (cyFBPase) can direct tissue, cell specific expression in transgenic rice. In order to identify sequence elements ...The 1 195 bp 5′ flanking region of rice ( Oryza sativa L.) cytosolic fructose_1, 6_bisphosphatase (cyFBPase) can direct tissue, cell specific expression in transgenic rice. In order to identify sequence elements responsible for the regulation of mesophyll_specific expression, the 5′ flanking regions of -1 195 bp, -1 102 bp, -768 bp, and -644 bp upstream of the translation initiation ATG codon were fused to the reporter gene encoding β_glucuronidase (GUS) and transferred to rice via particle bombardment. Analysis of the 5′ promoter deletions identified that a 93 bp fragment between -1 195 bp and -1 102 bp is essential for directing mesophyll specific expression. High constitutive expression of GUS reporter gene was found in the -768 deletion lines and another two deletion series. These results indicate the great potential utility of the promoter in rice biotechnology.展开更多
基金Supported by Startup Foundation for Doctors of Yangtze University~~
文摘The dynamic concentrations of glucose, fructose, sucrose and total sugar were determined through exogenous ABA and GA3 treatments during young period of fruit and before fruit coloring in pulp of ‘Cara cara’ navel orange. The results are as fol ows: 10 mg/L ABA increased glucose, fructose and total sugar concen-tration significantly or very significantly, ABA treatment of 50 mg/L increased sucrose concentration very significantly, but ABA treatment of 100 mg/L decreased glucose concentration very significantly. GA3 treatment of lower and middle concentrations(10, 50 and 250 mg/L) increased sucrose concentration very significantly, GA3 treatment of 10 mg/L had no remarkable effect on glucose and fructose concentration but in-creased total sugar concentration very significantly, GA3 treatment of 50, 250 and 500 mg/L decreased glucose, fructose and total sugar concentration very significant-ly. Therefore, ABA treatment of lower concentration could increase one or several kinds of sugar concentration, but GA3 treatment of higher concentration (250 and 500 mg/L) restrained sugar concentration in pulp of ‘Cara cara’ navel orange seri-ously.
文摘A full-length cDNA encoding fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from maize (Zea mays L.) was cloned by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and designated as mF2KP. The encoded protein is composed of two regions. Its COOH-terminal region is catalytic region and homologous to the enzymes from other eukaryotes; and its NH 2-terminal region is common and special region only in plant. A truncated fragment of mF2KP covering integrated catalytic region was expressed in Escherichia coli. The fusion protein had the activities of fructose-6-phosphate, 2-kinase as well as fructose-2,6-bisphosphatase. Northern blot showed that the transcript level of mF2KP in seedlings initiated from strong-vigor seeds is lower than that from weak-vigor seeds.
基金Supported by the Special Science Foundation of Jiangxi Province(20143ACF60015)National Natural Scientific Fund(31360472)~~
文摘[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6' kiwifruit (Actinidia eriantha Benth) were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as materials every 15 days since 20 days after full bloom (DAFB) to 170 DAFB until in mature stage. [Result] The results showed that during fruit development of ‘Ganmi 6', the total sugar soluble contents had a rising trend with relatively stable at 95 DAFB, then rose until har- vest with the maximum content (10.35%). The titratable acid content showed a trend of increasing, then declining, then increase to the harvest content (1.10%). From the sugar acid ratio, we can knew it decreased in 95 DAFB, then up to the max (9.38). The changes of AsA contents showed double ‘S' shape, decreased af- ter the first increased rapidly, slightly increased and then decreased in the early harvest. [Conclusion] It provided a theoretical basis for scientific cultivation methods to explore the nutrients regulation.
基金the Natural Science Foundation of Jiangsu Province (BK2003047)~~
文摘[ Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus. [ Method ] The free radical scavenging ability and reducing capacity of DPPH as well as inhibitory rate of nonenzymatic glycation reaction were measured targets for comparing changes of anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of fermentation lyophilizer and fermentation extraction before and after EGB fermention adsorbed by AB-8 macroporous resin. The EGB fermention was biotransformed by Hericium erinaceus. [ Result ] After adsorbed by AB-8 macroporous resin, the bioactive matters were concentrated and separated. The free radical scavenging rate, reducing capacity and inhibitory rate of nonenzymatic glycation reaction were increased significantly after adsorbed by AB-8 macroporous resin. [ Conclusion] AB-8 macroporous resin could be used for preliminary concentration of EGB fermentation which was biotransformed by Hericium erinaceus.
文摘Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Batsch cv. Feicheng) is described. The pMT18 clone is 1188 bp in length, with an open reading frame of 393 amino acids. The homology and phylogenetic analyses indicate a remarkable similarity between peach PG and other ripening related PG. And seven consensus sequences have revealed in peach PG compared to the PG from other plants. However, the profound divergence with other PG and the unique structure features suggest that peach PG probably belongs to a new evolutionary class. In RT PCR analysis, pMT18 related RNA was undetectable in leaves, and was much abundant in ripe fruits. The ripening specific expression pattern of this cDNA will be useful in investigating the roles of PG in fruit ripening and developing a transgenic peach with the improved post harvesting quality in the future.
文摘The variation of sugar, acid and vitamin C contents in fruits of red flesh kiwifruit (Actinidia chinensis) "Hongyang" and green fresh kiwifruit (A. deliciosa) 'Jinkui' were investigated during fruit development. The results showed that the to- tal sugar soluble contents of "Hongyang' and 'Jinkui" during fruit development ex- isted different variations. With the upward trend of 'Hongyang' fruit development, 95 days after full bloom (DAFB), the total soluble sugar accumulation was relatively slow, and then rose rapidly until harvest with the maximum content (6.87%). While 'Jinkui' fruit showed a fluctuant process, rising in 50 DAFB, then declining, then rising rapidly and decreasing slightly right before harvest. The variation of fruit titrat- able acid between them was more consistent, which was increased and then de- creased. The only difference was that the titratable acid content of 'Jinkui' fruit de- creased slowly from the late of fruit development to fruit ripening, similar to the maximum value, but that of 'Hongyang' fruit decreased rapidly in the late. Titrat- able acid contents of them in the maturity were 1.08% and 1.20%, respectively. The trends of sugar acid ratio for 'Hongyang' and 'Jinkui' fruit were quite different. 'Hongyang' fruit increased slowly and then rapidly; 'Jinkui' changed from decreas- ing to increasing, followed by slight decreasing in mature stage. In addition, the two kiwifruit varieties had a similar change trend of Vc content during fruit development, which changed from rapid increasing to declining, followed by slight growing in the harvest. The changing tendency of 'Jinkui' fruit was later than that of 'Hengyang'.
基金Supported by the Scientific and Technological Project of Yinchuan City, Ningxia Province~~
文摘[Objective] The aim was to study the preservation of fresh Chinese wolfberry fruit via chitosan coating. [Method] Using Chinese wolfberry "Ningqi No. 1" as experimental material, the fresh fruits were coated by different concentrations of chitosan(0.75%, 1.00%, 1.25%, 1.50% and 1.75%) at room temperature to study the changes in weight loss rate, rate of rotten fruits rot, soluble solid content and vitamin C(Vc) content. [Result] Of all the treatments, the fruits coated with 1.25% chitosan showed lowest rate of rotten fruit and weight loss rate, and highest Vc and soluble solid content, presenting the optimal fresh-keeping effect. [Conclusion] Chitosan coating is helpful for the preservation of fresh Chinese wolfberry fruit at room temperature.
基金supported by the Fundamental Research Funds for the Central Universities (TD2011-11,BLYJ201519)Beijing Higher Education Young Elite Teacher Project (YETP0765)+2 种基金National Natural Science Foundation of China (31170556)New Century Excellent Talents in University (NCET-13-0671)State Forestry Administration of China (201204803)~~
文摘A natural attapulgite (ATP)‐based catalyst, sulfated In2O3‐ATP (SO42-/In2O3‐ATP), was obtained by an impregnation‐calcination method and was used to efficiently and selectively produce the useful platform chemical 5‐hydroxymethylfurfural (HMF) from hexoses. Some important reaction param‐eters were studied, revealing that Lewis and Br-nsted acid sites on SO42-/In2O3‐ATP catalyze glu‐cose isomerization and fructose dehydration. The yields of HMF from glucose and fructose were 40.2%and 46.2%, respectively, using the optimal conditions of 180℃ for 60 min with 10 wt%of solid acid catalyst in a mixture of γ‐valerolactone‐water (9:1).
文摘Objective: To study the effects of sodium magnesiusm fructose diphosphate(FDPM) on brain damage of rats after ischemia-reperfusion. Methods: Rats were subjected to cerebral ischemia-reperfusion induced by inserting a nylon thread into internal carotid artery to block the origin of middle cerebral artery and removing the thread later. FDPM (400 mg·kg -1), fructose-1,6-diphosphate (FDP, 400 mg·kg -1)and magnesium sulfate (MgSO 4, 30 mg·kg -1) were administrated 10 min after the onset of ischemia. Neurological scale, brain infarct area, Malondialdehyde(MDA) content and histopathological changes of brain tissue were studied. Results: FDPM decreased neurological scale, diminished brain infarct area, reduced MDA content and relieved histopathological change of rat brain tissue subjected to ischemia-reperfusion. These effects were more powerful than that of FDP or MgSO 4. Conclusions: It is suggested that FDPM markedly prevented rats against brain damage after cerebral ischemia-reperfusion, and its effect was better than that of FDP or MgSO 4.
文摘The 1 195 bp 5′ flanking region of rice ( Oryza sativa L.) cytosolic fructose_1, 6_bisphosphatase (cyFBPase) can direct tissue, cell specific expression in transgenic rice. In order to identify sequence elements responsible for the regulation of mesophyll_specific expression, the 5′ flanking regions of -1 195 bp, -1 102 bp, -768 bp, and -644 bp upstream of the translation initiation ATG codon were fused to the reporter gene encoding β_glucuronidase (GUS) and transferred to rice via particle bombardment. Analysis of the 5′ promoter deletions identified that a 93 bp fragment between -1 195 bp and -1 102 bp is essential for directing mesophyll specific expression. High constitutive expression of GUS reporter gene was found in the -768 deletion lines and another two deletion series. These results indicate the great potential utility of the promoter in rice biotechnology.