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曹文轩儿童小说中的颜色词研究——以《草房子》《青铜葵花》《细米》为例 被引量:1
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作者 张文丽 《陕西学前师范学院学报》 2023年第9期118-124,共7页
选取中国当代著名儿童文学家曹文轩代表作《草房子》《青铜葵花》《细米》中的颜色词作为研究对象,通过搜集颜色词、分析颜色词的构成方式和在句子中承担的语法作用,发现小说中的颜色词的使用和基本颜色词理论基本契合;同时作为儿童文... 选取中国当代著名儿童文学家曹文轩代表作《草房子》《青铜葵花》《细米》中的颜色词作为研究对象,通过搜集颜色词、分析颜色词的构成方式和在句子中承担的语法作用,发现小说中的颜色词的使用和基本颜色词理论基本契合;同时作为儿童文学作品中的颜色词,其使用符合儿童认知规律和语言习得规律。 展开更多
关键词 《草房子》 《青铜葵花》 《细米》 颜色词
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踏上青春征途的骑士——《细米》主人公形象解读
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作者 马希武 马燕 《南京师范大学文学院学报》 CSSCI 2017年第2期72-75,共4页
曹文轩的《细米》被普遍认为是对此前类似作品的超越,主人公细米的人物形象更为复杂而多面。他和桑桑、林冰等乡村少年一样健康、聪慧、顽皮,却更多了一份细腻优雅和勇猛果敢。那么,如此多面的性格,为何能和谐地统一在一个人身上?这个... 曹文轩的《细米》被普遍认为是对此前类似作品的超越,主人公细米的人物形象更为复杂而多面。他和桑桑、林冰等乡村少年一样健康、聪慧、顽皮,却更多了一份细腻优雅和勇猛果敢。那么,如此多面的性格,为何能和谐地统一在一个人身上?这个问题并没有得到很好的回答。兼有温柔细腻的情怀、优雅的风度和勇猛的血性,其实正是一种西方骑士的形象。细米的骑士形象,能够从作品的情节中得到充分的印证。有了这个形象的统辖,细米性格中的多个侧面便不再冰火难容,而是和谐相通了。 展开更多
关键词 曹文轩 《细米》 骑士
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为少年心思写歌——评曹文轩的小说《细米》 被引量:7
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作者 李红叶 《盐城师范学院学报(人文社会科学版)》 2004年第1期66-70,共5页
曹文轩近年来所取得的文学成就 ,不仅在于他独树一帜的美学追求 ,还在于他探入了一个新的表现领域———少年情怀。《细米》格调优雅浪漫 ,以细腻的笔致描写了敏感多思的少年心思。《细米》有美的内容 ,也有美的形式 ,是当代文坛不可多... 曹文轩近年来所取得的文学成就 ,不仅在于他独树一帜的美学追求 ,还在于他探入了一个新的表现领域———少年情怀。《细米》格调优雅浪漫 ,以细腻的笔致描写了敏感多思的少年心思。《细米》有美的内容 ,也有美的形式 ,是当代文坛不可多得的美的收获之一。 展开更多
关键词 曹文轩 小说 《细米》 美学 文学风格 少年情怀
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一曲至真至纯至善的人性颂歌——读曹文轩《细米》所感
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作者 刘玉芳 李深 《现代语文(上旬.文学研究)》 2009年第8期131-132,共2页
曹文轩的长篇青春小说《细米》为我们呈现了至纯至善的人间真情。本文从作品主题、创作心理、情感处理方式三个维度透析至真至纯的人性颂歌。
关键词 曹文轩 《细米》 主题 创作心理 情感处理方式
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曹文轩《细米》的比喻辞格及其语用动因探析
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作者 杨雨菡 《柳州职业技术学院学报》 2018年第3期82-86,95,共6页
曹文轩先生的小说《细米》是一本极具美感的文学作品,言辞细腻,并且有大量修辞手法的运用,其中比喻的修辞手法刻画出一个个立体且鲜明的人物形象,并创造了唯美的意境。据统计,全文共出现了109例比喻辞格,其中明喻有75例,暗喻15例,借喻1... 曹文轩先生的小说《细米》是一本极具美感的文学作品,言辞细腻,并且有大量修辞手法的运用,其中比喻的修辞手法刻画出一个个立体且鲜明的人物形象,并创造了唯美的意境。据统计,全文共出现了109例比喻辞格,其中明喻有75例,暗喻15例,借喻19例。小说中比喻辞格的描写对象主要有人物、具象事物和抽象事物。作者认为,无论本体的选取还是喻体的选取,语用动因都是在符合儿童文学受众心理的前提下,为读者创造更大的联想空间和唯美意境。 展开更多
关键词 比喻辞格 儿童文学 曹文轩 《细米》
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浅谈《细米》人物形象塑造的独特性
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作者 张小军 《试题与研究(教学论坛)》 2010年第23期70-70,共1页
《细米》这本书属于“曹文轩纯美小说系列”。作者非常注重对于人物形象刻画的艺术表现手法,运用大量的语言、心理、环境描写刻画了人物鲜明的形象特征。整本书以一首民谣作为主题,全文连贯通畅,其中的各种人物角色也都刻画得淋漓尽... 《细米》这本书属于“曹文轩纯美小说系列”。作者非常注重对于人物形象刻画的艺术表现手法,运用大量的语言、心理、环境描写刻画了人物鲜明的形象特征。整本书以一首民谣作为主题,全文连贯通畅,其中的各种人物角色也都刻画得淋漓尽致,书中流淌着一股浓郁的乡土气息,而在对现实生活的精准描写之下,文学的浪漫之美依然表现得格外出色。 展开更多
关键词 人物形象塑造 《细米》 特性 艺术表现手法 人物形象刻画 环境描写 形象特征 人物角色
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稻花香---读曹文轩《细米》
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作者 许强 《作文与考试(高中版)》 2013年第28期25-26,共2页
风吹起如花般破碎的流年,而她的影子摇摇晃晃,成为他心中最美的风景。看天,看地,看青春季节里深深的暗影。细米与梅纹的相遇,从眼神交接的那一刻起,就注定是一段难以忘怀的记忆。
关键词 《细米》 曹文轩 流年 暗影
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细米
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作者 曹文轩 《海峡儿童(读写)(3-6年级)》 2010年第5期32-33,共2页
少年细米生来就是一个爱脸红的男孩儿,他与表妹红藕两小无猜,一同长大,日子如清水一般自然流淌。然而,有一天大河上飘来一叶巨大的白帆,一群仿佛来自天国的女孩儿给平静的乡村带来了一股新鲜而迷人的气息。
关键词 小学 课外阅读 阅读材料 《细米》
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Molecular dynamics study on temperature and strain rate dependences of mechanical tensile properties of ultrathin nickel nanowires 被引量:3
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作者 王卫东 易成龙 樊康旗 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第11期3353-3361,共9页
Based on the EAM potential, a molecular dynamics study on the tensile properties of ultrathin nickel nanowires in the (100〉 orientation with diameters of 3.94, 4.95 and 5.99 nm was presented at different temperature... Based on the EAM potential, a molecular dynamics study on the tensile properties of ultrathin nickel nanowires in the (100〉 orientation with diameters of 3.94, 4.95 and 5.99 nm was presented at different temperatures and strain rates. The temperature and strain rate dependences of tensile properties were investigated. The simulation results show that the elastic modulus and the yield strength are gradually decreasing with the increase of temperature, while with the increase of the strain rate, the stress--strain curves fluctuate more intensely and the ultrathin nickel nanowires rupture at one smaller and smaller strain. At an ideal temperature of 0.01 K, the yield strength of the nanowires drops rapidly with the increase of strain rate, and at other temperatures the strain rate has a little influence on the elastic modulus and the yield strength. Finally, the effects of size on the tensile properties of ultrathin nickel nanowires were briefly discussed. 展开更多
关键词 ultrathin nickel nanowires temperature dependence strain rate dependence tensile properties molecular dynamics simulation
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Microstructure refinement of a dual phase titanium alloy by severe room temperature compression 被引量:1
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作者 张志强 董利民 +3 位作者 杨洋 关少轩 刘羽寅 杨锐 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第11期2604-2608,共5页
Microstructure refinement of a dual phase titanium alloy, Ti-3AI-4.5V-5Mo, by severe room temperature compression was investigated. Nanocrystalline grains were observed in the sample with 75% reduction, in which the g... Microstructure refinement of a dual phase titanium alloy, Ti-3AI-4.5V-5Mo, by severe room temperature compression was investigated. Nanocrystalline grains were observed in the sample with 75% reduction, in which the grain sizes of a phase and β phase were approximately 50 and 100 nm. Conversely, the average thicknesses of a phase and β phase in as-received microstructure were measured to be 0.7 and 0.5 μm, respectively. TEM and XRD methods were used to analyze the microstructure and texture changes after severe deformation. Microstructure refinement was deduced to the complex interaction among slip dislocations in the a phase, the complex interaction among slip dislocations and martensites in the β phases. In addition, the interaction between the a phase and the β phase also contributed to the microstructure refinement. 展开更多
关键词 dual phase titamum alloy Ti-3AI-4.3V-3Mo alloy severe plastic detormatlon mlcrostructure retinement nanocrystalline grains TEXTURE
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恬淡微妙的爱情萌动与物质化下的情感追求
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作者 郑楚云 《现代语文(上旬.文学研究)》 2008年第2期121-121,共1页
一、前言 人的一生中不能没有爱情,《易经》里说“天地氤氲,万物化醇;男女构精,万物化生。”可见爱情的重要,古往今来,几乎人间所有的悲欢离合都和它有关。爱情,是人类生活中不可缺少的一部分。尤其是在《细米》成长中的那一股... 一、前言 人的一生中不能没有爱情,《易经》里说“天地氤氲,万物化醇;男女构精,万物化生。”可见爱情的重要,古往今来,几乎人间所有的悲欢离合都和它有关。爱情,是人类生活中不可缺少的一部分。尤其是在《细米》成长中的那一股最初的爱情萌动——它是敏感的,同时也是美丽的:它是柔和的,同时也是抒情的。然而,对比文本中的纯美情感,我们不难发现在物质化的今天,感情的纯真浪漫正经受着世俗世界的莫大挑战与考验。 展开更多
关键词 物质化 爱情 情感 《易经》 人类生活 《细米》 物化 天地
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Effects of Nanometer Hydroxyapatite on Proliferation of Periodontal Ligament Fibroblasts 被引量:2
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作者 孙卫斌 吴亚菲 +1 位作者 丁一 刘天佳 《Journal of Nanjing Medical University》 2004年第6期288-292,共5页
Objective:To investigate possible effects of nanophase powder of hydroxyapatite on proliferation of periodontal ligament cells. Methods: With sol-gel method, the nanophase hydroxyapatite powders were fabricated. These... Objective:To investigate possible effects of nanophase powder of hydroxyapatite on proliferation of periodontal ligament cells. Methods: With sol-gel method, the nanophase hydroxyapatite powders were fabricated. These powders were proved nanopaticles by transmission electron microscope. The effects on proliferation of periodontal ligament cell(PDLC) were observed in vitro with MTT [3-(4,5dimethylthiazo;-2-yl)-2,5-diphenytetralium bromide] method. Results: On the 2nd,3rd,4th day after treated with nanoparticles of hydroxyapatite, the proliferate activity of the PDLC increases significantly, compared with those with dense hydroxyaoatite and control but no significant difference could be found between the dense hydroxyapatite and the control. Conclusion: Nanophase hydroxyapatite can promote the regeneration of periodontal tissue. 展开更多
关键词 HYDROXYAPATITE nanoparticle material periodontal disease
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In Vitro Fertilization in Maize and Tobacco: The Fusion Patterns During Sexual Cell Fusion 被引量:1
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作者 孙蒙祥 杨弘远 Mauro CRESTI 《Acta Botanica Sinica》 CSCD 2002年第10期1142-1145,共4页
Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns cou... Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns could be recognized in sexual cell fusion, which was supported by fluorescent microscopy with the aid of video enhanced system and cooled CCD. It was found that the fusion pattern and cell membrane behavior were related to the cell volume ratio of two fusing cells. The results reported here might be useful for interpreting and evaluating data from in vitro fertilization experiments and for explaining male cytoplasm exclusion during fertilization. 展开更多
关键词 in vitro fertilization MAIZE TOBACCO cell volume fusion pattern FITC-Con A
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Analysis of Heterosis, Combining Ability and Heritability of Cadmium Content in Brown Rice of Three-line Indica Hybrid Rice
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作者 游书梅 曹应江 +8 位作者 蒋开锋 郑家奎 吴先军 张涛 杨莉 杨乾华 万先齐 罗婧 李昭祥 《Agricultural Science & Technology》 CAS 2012年第11期2358-2362,共5页
Five cytoplasmic male sterile (CMS) lines were used as parents in an incomplete diallet cross and six restorer lines of rice design (Nc II design). Thirty hybrid combinations with the same growth period were selec... Five cytoplasmic male sterile (CMS) lines were used as parents in an incomplete diallet cross and six restorer lines of rice design (Nc II design). Thirty hybrid combinations with the same growth period were selected as experimental ma- terials to investigate the heterosis, combined ability and heredity of Cd content in brown rice of indica hybrid rice. According to the results, Cd content in brown rice showed a significantly negative heterosis; the general combining ability and specific combination ability of Cd content in CMS and restorer lines both reached extremely significant level (P〈0.01), indicating that both genetic improvement of parents and e- valuation of combinations are important to the breeding of hybrid combinations with low accumulation of Cd; the broad-sense heritabitity and narrow-sense heritability of Cd content were both relatively high with slight differences, which respectively reached 97.73% and 80.10%, indicating that Cd content in brown rice mainly de- pends on the additive action of genes; in addition, parent improvement showed bet- ter effect on the selection of early generation. 展开更多
关键词 Indica hybrid rice Brown rice Cytoplasmic male sterile (CMS) line Re-storer line Cadmium HETEROSIS Combining ability HERITABILITY
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Effect of nano TiN/Ti refiner on as-cast and hot-working microstructure of commercial purity aluminum 被引量:4
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作者 蔡思祺 李玉洁 +2 位作者 谌援 李欣蔚 薛丽红 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第7期1890-1897,共8页
A novel type nano TiN/Ti composite grain refiner (TiN/Ti refiner) was prepared by high energy ball milling, and its effect on as-cast and hot-working microstructure of commercial purity aluminum (pure Al) was inve... A novel type nano TiN/Ti composite grain refiner (TiN/Ti refiner) was prepared by high energy ball milling, and its effect on as-cast and hot-working microstructure of commercial purity aluminum (pure Al) was investigated. The results show that TiN/Ti refiner exhibits excellent grain refining performances on pure Al. With an addition of 0.2% TiN/Ti refiner, the average grain size of pure Al decreases to 82 μm, which is smaller than that of pure Ti and Al 5Ti 1B master alloy as refiners. The microstructure of weld joint of pure Al with 0.1% TiN/Ti refiner is fine equiaxed grains and the hardness of weld joint is higher than that of the base metal. For pure Al with 40% cold deformation and recrystallization at 250 °C for 1.0 h, the grains of the sample added 0.1% Ti powder have an obvious grain growth behavior. In contrast, oriented grains caused by deformation have been eliminated, and there is no obvious grain growth in pure Al refined with 0.1% TiN/Ti refiner, indicating that nano TiN in the refiner inhibits the growth of grain during recrystallization. 展开更多
关键词 nano TiN/Ti composite grain refiner commercial purity aluminum grain refinement weld joint deformation and recrystallization
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Distance-Dependent Long-Range Electron Transfer in Protein:a Case Study of Photosynthetic Bacterial Light-Harvesting Antenna Complex LH2 Assembled on TiO 2 Nanoparticle by Femto-Second Time-Resolved Spectroscopy
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作者 翁羽翔 张蕾 +9 位作者 杨健 全冬晖 汪力 杨国桢 藤井律子 小山泰 张建平 冯娟 余军华 张宝文 《Acta Botanica Sinica》 CSCD 2003年第4期488-493,共6页
The function of protein in long-range biological electron transfer is a question of debate. We report some preliminary results in femtosecond spectroscopic study of photosynthetic bacterial light-harvesting antenna co... The function of protein in long-range biological electron transfer is a question of debate. We report some preliminary results in femtosecond spectroscopic study of photosynthetic bacterial light-harvesting antenna complex assembled onto TiO2 nanoparticle with an average size of 8 nm in diameter. Crystal structure shows that photosynthetic bacterial antenna complex LH2 has a ring-like structure composed by alpha- and beta-apoprotein helices. The alpha- and beta-transmembrance helices construct two concentric cylinders with pigments bacteriochlorophyll a (Bchl a) and carotenoid (Car) buried inside the protein. We attempt to insert TiO2 nanoparticle into the cavity of the inner cylindrical hollow of LH2 to investigate the nature of the electron transfer between the excited-state Bchl a and the TiO2 nanoparticle. A significant decrease in the ground state bleaching recovery time constant for Bchl a at 850 run (B850) in respect to that of the Bchl a in free LH2 has been observed. By using the relation of distance-dependent long-range electron transfer rate in protein, the distance between the donor B850 and the acceptor TiO2 nanoparticle has been estimated, which is about 0.6 nm. The proposed method of assembling proteins onto wide-gap semiconductor nanoparticle can be a promising way to determine the role of the protein playing in biological electron transfer processes. 展开更多
关键词 TiO2 nanoparticle LH2 time-resolved spectroscopy charge transfer energy transfer protein
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Antineoplastic Effect of Calcium Channel Blocker-Verapamil and 5-Fluorouracil Intraperitoneal Chemotherapy on Hepatocarcinoma-Bearing Rats
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作者 曹天生 史海安 周亚魁 《The Chinese-German Journal of Clinical Oncology》 CAS 2002年第2期84-87,共4页
Objective To study the antineoplastic effect of the calcium channel blocker verapamil and 5-fluorouracil intraperitoneal chemotherapy onhepatocarcinoma-bearing rats, and examine the action between calcium channel bloc... Objective To study the antineoplastic effect of the calcium channel blocker verapamil and 5-fluorouracil intraperitoneal chemotherapy onhepatocarcinoma-bearing rats, and examine the action between calcium channel blockers and cytotoxic drugs.Methods We adopted the method of subcapsular implantation of carcinoma tissues of walker-256 in the left liver lobe as a model of livercarcinoma-bearing rats. All experimental animals were divided into four groups. On the sixth day post implantation, in group A (controlgroup) 6 ml of saline was injected intraperitoneally once a day for 3 days. In group B (single chemotherapy group) 6 ml of 5-Fu 75 mg/kg was injected intraperitoneally once a day for 3 days. In group C (combination of treatment group) both 5-Fu (75 mg/kg) and verapamil(25 mg/kg) were administered simultaneously as in A and B. In group D (simple verapamil group) only 6 ml of verapamil (25 mg/kg)was administered as above.Results Compared with groups A, B and D, The volume of cancer and the contents of liver cancer DNA and protein were significantlyreduced. The rates of inhibiting cancer (89.9% in group C and 35.4% in group B) were significantly increased in group C. Group C hadsignificantly long survival time compared to groups A, B and D ( P < 0.05) . By light microscopy, a number of focal necroses were foundin cancer tissue in group C.Conclusion Calcium channel blockers can enhance the antineoplastic effect of 5-Fu intraperitoneal chemotherapy to liver cancer ; Theuse of verapamil can not increase the toxicity of 5-Fu. 展开更多
关键词 calcium channel blockers VERAPAMIL 5-FLUOROURACIL HEPATOCARCINOMA intraperitoneal chemotherapy
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Developmental characteristics and response to iron toxicity of root border cells in rice seedlings 被引量:9
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作者 Cheng-hua XING Mei-hong ZHU +3 位作者 Miao-zhen CAI Peng LIU Gen-di XU Shao-hui WU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第3期261-264,共4页
To investigate the Fe^2+ effects on root tips in rice plant, experiments were carried out using border cells in vitro. The border cells were pre-planted in aeroponic culture and detached from root tips. Most border c... To investigate the Fe^2+ effects on root tips in rice plant, experiments were carried out using border cells in vitro. The border cells were pre-planted in aeroponic culture and detached from root tips. Most border cells have a long elliptical shape. The number and the viability of border cells in situ reached the maxima of 1600 and 97.5%, respectively, at 20---25 mm root length. This mortality was more pronounced at the first 1-12 h exposure to 250 mg/L Fe^2+ than at the last 12-36 h. After 36 h, the cell viability exposed to 250 mg/L Fe^2+ decreased to nought, whereas it was 46.5% at 0 mg/L Fe^2+. Increased Fe^2+ dosage stimulated the death of detached border cells from rice cultivars. After 4 h Fe^2+ treatment, the cell viabilities were _〉80% at 0 and 50 mg/L Fe^2+ treatment and were 〈62% at 150, 250 and 350 mg/L Fe^2+ treatment; The viability of border cells decreased by 10% when the Fe^2+ concentration increased by 100 mg/L. After 24 h Fe^2+ treatment, the viabilities of border cells at all the Fe^2+ levels were 〈65%; The viability of border cells decreased by 20% when the Fee+ concentration increased by 100 mg/L. The decreased viabilities of border cells indicated that Fe^2+ dosage and treatment time would cause deadly effect on the border cells. The increased cell death could protect the root tips from toxic harm. Therefore, it may protect root from the damage caused by harmful iron toxicity. 展开更多
关键词 Rice plant Border cells Iron toxicity
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Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis 被引量:29
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作者 Xi-Ping Zhang Li Chen +10 位作者 Qi-Fang Hu Hua Tian Ru-Jun Xu Zhi-Wei Wang Ke-Yi Wang Qi-Hui Cheng Wei Yan Yun Li Qing-Yu Li Qing He Fei Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第41期5506-5511,共6页
AIM: To investigate the influence of high dose of dexamethasone on inflammatory mediators and apoptosis of rats with severe acute pancreatitis (SAP). METHODS: SAP rats were randomly assigned to the model group and tre... AIM: To investigate the influence of high dose of dexamethasone on inflammatory mediators and apoptosis of rats with severe acute pancreatitis (SAP). METHODS: SAP rats were randomly assigned to the model group and treatment group while the normal rats were assigned to the sham operation group. The mortality,ascite volumes,ascites/body weight ratio and pancreas pathological changes of all rats were observed at 3,6 and 12 h after operation. Their contents of amylase and endotoxin in plasma and contents of tumor necrosis factor (TNF-α),phospholipase A2 (PLA2) and IL-6 in serum were also determined. The microarray sections of their pancreatic tissues were prepared,terminal transferase dUTP nick end labeling (TUNEL) staining was performed and apoptotic indexes were calculated. RESULTS: There was no marked difference between treatment group and model group in survival. The contents of amylase and endotoxin in plasma and contents of TNF-α,PLA2 and IL-6 in serum,ascite volumes,ascites/body weight ratio and pancreas pathological scores were all lower in treatment group than in model group to different extents at different time points P < 0.05,58.3 (26.4) ng/L vs 77.535 (42.157) ng/L in TNF-α content,8.00 (2.00) points vs 9.00 (2.00) points in pathological score of pancreas respectively; P < 0.01,0.042 (0.018) EU/mL vs 0.056 (0.0195) EU/mL in endotoxin content,7791 (1863) U/L vs 9195 (1298) U/L in plasma amylase content,1.53 (0.79) vs 2.38 (1.10) in ascites/body weight ratio,8.00 (1.00) points vs 11.00 (1.50) points in pathological score of pancreas; P < 0.001,3.36 (1.56) ng/L vs 5.65 (1.08) ng/L in IL-6 content,4.50 (2.00) vs 7.20 (2.00),4.20 (1.60) vs 6.40 (2.30),3.40 (2.70) vs 7.90 (1.70) in ascite volumes,respectively. The apoptotic indexes of pancreas head and pancreas tail were all higher in treatment group than in model group at 6 h P < 0.01,0.00 (2.00)% vs 0.00 (0.00)%,0.20 (1.80) vs 0.00 (0.00) in apoptosis indexes,respectively. CONCLUSION: The mechanism of dexamethasone treatment in acute pancreatitis is related to its inhibition of inflammatory mediator generation and induction of pancreatic acinar cell apoptosis. 展开更多
关键词 Severe acute pancreatitis APOPTOSIS Inflammatory mediators DEXAMETHASONE Tissue microarrays
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Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone 被引量:25
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作者 LiYANG JinBoYANG +8 位作者 JiaCHEN GuangYaoYU PeiZHOU LeiLEI ZhenZhenWANG CatherineCYCHANG XinYingYANG TaYuanCHANG BoLiangLI 《Cell Research》 SCIE CAS CSCD 2004年第4期315-323,共9页
In macrophages, the accumulation of cholesteryl esters synthesized by the activated acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT1) results in the foam cell formation, a hallmark of early atherosclerotic lesions... In macrophages, the accumulation of cholesteryl esters synthesized by the activated acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT1) results in the foam cell formation, a hallmark of early atherosclerotic lesions. In this study, with the treatment of a glucocorticoid hormone dexamethasone (Dex), lipid staining results clearly showed the large accumulation of lipid droplets containing cholesteryl esters in THP-1-derived macrophages exposed to lower concentration of the oxidized low-density lipoprotein (ox-LDL). More notably, when treated together with specific anti-ACAT inhibitors, the abundant cholesteryl ester accumulation was markedly diminished in THP-1-derived macrophages, confirming that ACAT is the key enzyme responsible for intracellular cholesteryl ester synthesis. RT-PCR and Western blot results indicated that Dex caused up-regulation of human ACAT1 expression at both the mRNA and protein levels in THP-1 and THP-1-derived macrophages. The luciferase activity assay demonstrated that Dex could enhance the activity of human ACAT1 gene P1 promoter, a major factor leading to the ACAT1 activation, in a cell-specific manner. Further experimental evidences showed that a glucocorticoid response element (GRE) located within human ACAT1 gene P1 promoter to response to the elevation of human ACAT1 gene expression by Dex could be functionally bound with glucocorticoid receptor (GR) proteins. These data supported the hypothesis that the clinical treatment with Dex, which increased the incidence of atherosclerosis, may in part due to enhancing the ACAT1 expression to promote the accumulation of cholesteryl esters during the macrophage-derived foam cell formation, an early stage of atherosclerosis. 展开更多
关键词 ACAT DEXAMETHASONE MACROPHAGE cholesteryl ester gene promoter.
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