An in vitro shoot regeneration procedure was developed in pepper （ Capsicum annuum L. ） cytoplasmic male sterility （CMS） lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from co...An in vitro shoot regeneration procedure was developed in pepper （ Capsicum annuum L. ） cytoplasmic male sterility （CMS） lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog （MS） medium supplemented with different combinations of 6-benzladenine （6-BA）, indole-3-acetic acid （IAA）, gibberellic acid （GA3） and silver nitrate （AgNO3）. From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates （ respectively 100.0% and 58.3% ） and average number of adventitious bud from each explant （respectively 18.8 and 13.2） were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be： MS ＋ 3.0 mg/L 6-BA ＋ 1.0 mg/L IAA ＋ 5.0 mg/L AgNO3 ＋ 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA.展开更多
In order to understand the microtubule change of monocotyls stem-tip during mitosis, the arrangement, transformation of microtubule array and its relation with chromosome movement during mitosis were studied with free...In order to understand the microtubule change of monocotyls stem-tip during mitosis, the arrangement, transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome, indirect immunofluoreseenee, DAPI staining and fluorescence microscopy. The results showed that nucleolus was intact when the cortical microtubules formed; cortical microtubules were changed into phramoplast microtubules bands at mitosis prophase. When phramoplast microtubules came into being, nuclear membrane was ruptured and chromosome was arranged at the position of cell plate ; subsequently, phramoplast microtubules were changed into phragmoplast microtubules, phramoplast microtubules were shortening and microtubules on the sides of cell plate were increasing gradually, during this course sister ehromatid was separated by microtubules at cell plate and tract to the two poles, forming phragmoplast microtubules. Then the nucleolus of two daughter cells formed and separated in the end with the increase of cells numbers. Therefore, cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status.展开更多
Changes in the pattern of organization of microtubules in the meiotic stages of development of pollen (i.e. from pre-meiotic interphase to more or less metaphase I) of a normal (IR36) and a temperature/photoperiod sen...Changes in the pattern of organization of microtubules in the meiotic stages of development of pollen (i.e. from pre-meiotic interphase to more or less metaphase I) of a normal (IR36) and a temperature/photoperiod sensitive male sterile line (Peiai 64S) of rice were studied using immunofluorescence confocal microscopy. In IR36, from pre-meiotic interphase to metaphase I, the pattern of microtubule distribution in the meiocytes underwent a series of changes. Some new organizational patterns of microtubules (that have not been described before) were observed during microsporogenesis, including the existence of a broad band of perinuclear microtubules at the diakinesis stage of development. The pattern of microtubule distribution in the meiocytes of the male sterile line, Peiai 64S, was quite different front that seen in IR36. In Peiai 64S, the microtubules showed abnormal patterns of distribution from pre-meiotic interphase to metaphase I. For example the broad band of perinuclear microtubules seen at diakinesis in IR36 was much disorganized and loosened in Peiai 64S. The spindles formed were also very abnormal and different from the normal spindle. The appearance of abnormal microtubule distribution in the early stages of microsporogenesis may contribute to the malformation and ultimate abortion of pollen in Peiai 64S.展开更多
Cytokinins and Ca 2+ singly retarded senescence of detached rice leaves.When Ca 2+ was applied together with cytokinins,the effectiveness of cytokinins was significantly reduced.Ca 2+ and cytokinins did ...Cytokinins and Ca 2+ singly retarded senescence of detached rice leaves.When Ca 2+ was applied together with cytokinins,the effectiveness of cytokinins was significantly reduced.Ca 2+ and cytokinins did not stimulate ethylene production synergistically,ruling out the possibility that ethylene was involved in the inhibition of cytokinin induced senescence retarding effect by Ca 2+ .The experiment with specific compounds known to increase (Ca ionophore A23187),or decrease (EGTA,LaCl 3,Verapamil,chlorpromazine) cytosolic Ca 2+ level indicated that the elevated cytosolic Ca 2+ retards senescence.展开更多
基金Supported by "863" High Tech Project of China (2001AA241121-10) Natural Science Foundation of Yunnan Province (2005C0023Q)~~
文摘An in vitro shoot regeneration procedure was developed in pepper （ Capsicum annuum L. ） cytoplasmic male sterility （CMS） lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog （MS） medium supplemented with different combinations of 6-benzladenine （6-BA）, indole-3-acetic acid （IAA）, gibberellic acid （GA3） and silver nitrate （AgNO3）. From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates （ respectively 100.0% and 58.3% ） and average number of adventitious bud from each explant （respectively 18.8 and 13.2） were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be： MS ＋ 3.0 mg/L 6-BA ＋ 1.0 mg/L IAA ＋ 5.0 mg/L AgNO3 ＋ 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA.
基金Supported by the National Natural Science Foundation of China(30060038)~~
文摘In order to understand the microtubule change of monocotyls stem-tip during mitosis, the arrangement, transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome, indirect immunofluoreseenee, DAPI staining and fluorescence microscopy. The results showed that nucleolus was intact when the cortical microtubules formed; cortical microtubules were changed into phramoplast microtubules bands at mitosis prophase. When phramoplast microtubules came into being, nuclear membrane was ruptured and chromosome was arranged at the position of cell plate ; subsequently, phramoplast microtubules were changed into phragmoplast microtubules, phramoplast microtubules were shortening and microtubules on the sides of cell plate were increasing gradually, during this course sister ehromatid was separated by microtubules at cell plate and tract to the two poles, forming phragmoplast microtubules. Then the nucleolus of two daughter cells formed and separated in the end with the increase of cells numbers. Therefore, cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status.
文摘Changes in the pattern of organization of microtubules in the meiotic stages of development of pollen (i.e. from pre-meiotic interphase to more or less metaphase I) of a normal (IR36) and a temperature/photoperiod sensitive male sterile line (Peiai 64S) of rice were studied using immunofluorescence confocal microscopy. In IR36, from pre-meiotic interphase to metaphase I, the pattern of microtubule distribution in the meiocytes underwent a series of changes. Some new organizational patterns of microtubules (that have not been described before) were observed during microsporogenesis, including the existence of a broad band of perinuclear microtubules at the diakinesis stage of development. The pattern of microtubule distribution in the meiocytes of the male sterile line, Peiai 64S, was quite different front that seen in IR36. In Peiai 64S, the microtubules showed abnormal patterns of distribution from pre-meiotic interphase to metaphase I. For example the broad band of perinuclear microtubules seen at diakinesis in IR36 was much disorganized and loosened in Peiai 64S. The spindles formed were also very abnormal and different from the normal spindle. The appearance of abnormal microtubule distribution in the early stages of microsporogenesis may contribute to the malformation and ultimate abortion of pollen in Peiai 64S.
基金Supported by the N tional Natural Science Foundation of China
文摘Cytokinins and Ca 2+ singly retarded senescence of detached rice leaves.When Ca 2+ was applied together with cytokinins,the effectiveness of cytokinins was significantly reduced.Ca 2+ and cytokinins did not stimulate ethylene production synergistically,ruling out the possibility that ethylene was involved in the inhibition of cytokinin induced senescence retarding effect by Ca 2+ .The experiment with specific compounds known to increase (Ca ionophore A23187),or decrease (EGTA,LaCl 3,Verapamil,chlorpromazine) cytosolic Ca 2+ level indicated that the elevated cytosolic Ca 2+ retards senescence.
