Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of it...Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of its 1H, 13C NMR and EI-MS spectral data. It could be inferred that the enzyme responsible for the biotransforma-tion reaction was a kind of extracellular and constitutive enzyme since the transformation reaction of the substrate could be carried out in cell free extracts of the fermentation broth of the Mucor spinosus.展开更多
Ultrastructural changes in the sieve element and dense cells of nectariferous tissue during the development of floral nectary in Arabidopsis thaliana L. were investigated with transmission electron microscopy. Samples...Ultrastructural changes in the sieve element and dense cells of nectariferous tissue during the development of floral nectary in Arabidopsis thaliana L. were investigated with transmission electron microscopy. Samples were prepared with high pressure freezing and freeze substitution techniques. The ultrastructure of dense cell was similar to that of sieve element at its early developmental stage. With the concurrent agglutination of chromatin in the nucleus, the abnormal location of organelles and the high density of cytoplasm, the ultrastructural characteristics in die dense cells of the nectariferous tissue and in the sieve element are matched with those of the programmed cell death in animal and plant reported in recent years. The disorganization of nucleus and most organelles in the differentiation of sieve elements and dense cells is closely associated with the transportation and modification of pre-nectar and the transference of nectar. This suggests that the cytological changes in sieve element and nectariferous tissue are closely associated with the nectary functional activities.展开更多
[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extru...[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.展开更多
Anthers of male sterile line and its maintainer line of Zidao type rice, Yingxiang A and Yingxiang B were studied by cytological methods, such as F-KI staining, silver nitrate staining and paraffin sections, It is fou...Anthers of male sterile line and its maintainer line of Zidao type rice, Yingxiang A and Yingxiang B were studied by cytological methods, such as F-KI staining, silver nitrate staining and paraffin sections, It is found that in Yingxiang A, there are some abnormalities in meiosis, represented by unequal division at the meiotic phase; the abortion of CMS line arises at the meiotic phase and is almost completed at the phase of monomicrospore, but only a tiny amount of aborted mi- crospores are observed; the abortion is complete and thorough, and none normal functional pollens are found; the morphology of aborted pollens is similar to that of typically aborted pollens; the tapetal cells still exist in anthers from the microsporocyte stage to the tetrad stage, but they are abnormally hyperplastic. However, in Yingxiang B, there are no abnormalities in meiosis at the meiotic phase; the tapetal cells exist in anthers before the tetrad stage, but they disappear completely when the pollen are mature. It is indicated that the abortion of CMS Yingxiang line mainly occurs at the meiotic phase, and is basically completed at the phase of monomicrospore. The abortion is probably caused by the abnormally hyperplastic tapetal cells. The abnormalities in tapetal cells may affect the normal development of pollens, resulting in the emergence of abortion. In addition, the abortion in Yingxiang A is complete and thorough, which is of great significance in hybrid breeding.展开更多
Objective： To block the apoptosis of T lymphocytes induced by Fas/FasL in order to establish a method of the large-scale preparation of large amounts of tumor-specific cytoxic T-lymphocytes （CTL）. Methods： Liver c...Objective： To block the apoptosis of T lymphocytes induced by Fas/FasL in order to establish a method of the large-scale preparation of large amounts of tumor-specific cytoxic T-lymphocytes （CTL）. Methods： Liver cancer cells and tumor infiltrating lymphocytes （TIL） were isolated from FasL positive fresh specimens, and co-cultured. Specific CTL were activated and prepared in the presence of the co-stimulation of monoclonal antibody CD28. Then the blocking and activation of apoptosis of T lymphocytes was activated by soluble Fas receptor, which was detected by cytometry and DNA ladder test simultaneously. The apoptosis-blocking effect was compared with the control group. Furthermore, the changes of T cell proliferation and killing activity were detected by the method of ^3H thymidine incorporation and ^51Cr release test. Results： There was a significant increase in apoptosis rate in unblocking group compared with blocking group and quiescent group, with the unblocking group of 47.82%±0.13%, quiescent group of 3.76%±0.25%, and the blocking group of 8.22%±0.26% respectively （P〈0.01）. T cell-ladder appeared in unblocking group by DNA ladder test. Both the killing ability and proliferation rate of T cells were significantly increased after blocking. There was significant difference among blocking group, unblocking group and quiescent group （P〈0.01）. Conclusion： With this method we obtained large amounts of tumor-specific T lymphocytes, which was able to kill liver cancer cells effectively.展开更多
Scope Cell Research is an international journal publishing peer- reviewed original articles, invited reviews, commentaries, and letters to the editor. Original articles report significant original basic research that ...Scope Cell Research is an international journal publishing peer- reviewed original articles, invited reviews, commentaries, and letters to the editor. Original articles report significant original basic research that describe novel molecular and cellular processes and events and/or address the underlying biological mechanisms. Reviews and Commentaries review or comment on current progress in any field covered by the journal. Reviews are generally invited in advance by the editors. Letters to the editor report novel findings that have an immediate major impact on current biological research with only one figure and less than 10 references.展开更多
It has been 15 years since Cell Research was launched in 1990. At that time, Chinese scientists rarely published their work in non-Chinese journals, and China did not have a major English journal of its own in life sc...It has been 15 years since Cell Research was launched in 1990. At that time, Chinese scientists rarely published their work in non-Chinese journals, and China did not have a major English journal of its own in life sciences. In a way, Cell Research was born at the right time, in the right place, and with the right kind of people guiding its development. The rise of Cell Research as China＇s leading journal publishing peer-reviewed articles in basic life sciences has paralleled the rapid development of the Chinese economy in general and the science and technology sector in particular over the same period. The current economic trend should allow even more rapid advances in the life sciences which will ensure a healthy growth of Cell Research in years to come. Thus, we are confident that Cell Research has a brighter future.展开更多
2016年4月22日，浙江大学医学院李晓明教授团队在《细胞研究》（Cell Research）上发表题为“ Loss of MeCP2 in eholinergic neurons causes part of RTr - like phenotypes via a7 receptor in hippocampus＂ （ http：//www, nature,...2016年4月22日，浙江大学医学院李晓明教授团队在《细胞研究》（Cell Research）上发表题为“ Loss of MeCP2 in eholinergic neurons causes part of RTr - like phenotypes via a7 receptor in hippocampus＂ （ http：//www, nature, com/cr/journal/ v26/n6/abs/cr201648a, html）的论文，报道了胆碱能系统通过调控γ-氨酪酸在瑞特综合征发病机制中发挥作用，并提出胡乙酰胆碱受体可能是治疗瑞特综合征的潜在靶点。展开更多
Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to th...Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to the lack of systematic study. In recent years, the importance of Coix has been realized with the exploration of its effects in treating and preventing cancer. The systematic study of Coix has met a superb development opportunity with the rapid development of biotechnology especially the deep-sequencing technique. Therefore, this paper presented here the current status of origin and evolution, classification, cytology and molecular biology progress of Coix, analyzed the ploidy of Coix and the genetic relationships with Zea, Sorghum and so on from the point of cytology, and then based on the molecular biology, analyzed the important significance of DNA molecular marker, genetic mapping and gene library in the research on identification of genetic relationship between species and genetic breeding, which could provide useful information for further Coix research.展开更多
Cell Research, an international peer-reviewed journal in English, publishes results of significance and originality in all disciplines of cell biology and molecular cell biology. The journal is indexed in PubMed, Inde...Cell Research, an international peer-reviewed journal in English, publishes results of significance and originality in all disciplines of cell biology and molecular cell biology. The journal is indexed in PubMed, Index Medicus/MEDLINE, Science Citation Index, Current Contents-Life Sciences,SciSearch, ISI Web of Science, BIOSIS, CA, VINITI, ChinaInfo(CD & Web), Chinese Journal Full-text Database (CJFD) and Chinese Electronic Periodicals Service (CEPS) et al.展开更多
CellResearch为中国科学院上海生命科学研究院主办，以全英文刊登国内外细胞生物学及其相关领域的原创性研究论文、综述、快报和述评的国际性期刊。已被Index Medicus，Medline，SCI（SciSearch ISI Web of Science），BIOSIS，CA，VINI...CellResearch为中国科学院上海生命科学研究院主办，以全英文刊登国内外细胞生物学及其相关领域的原创性研究论文、综述、快报和述评的国际性期刊。已被Index Medicus，Medline，SCI（SciSearch ISI Web of Science），BIOSIS，CA，VINIT以及《中国科学引文数据库》、《万方数据库》（光盘版，网络版）、《中国英文版科技期刊数据库》、《中文科技期刊数据库》、《中国学术期刊》（光盘版，网络版）、展开更多
Welcome to Cell Research Cell Research covers a broad scope in basic molecular and cell biology research, including cell growth and differentiation, signal transduction, apoptosis, stem cells, development, immunology,...Welcome to Cell Research Cell Research covers a broad scope in basic molecular and cell biology research, including cell growth and differentiation, signal transduction, apoptosis, stem cells, development, immunology, neurosciences, plant cell biology, chromatin modulation, epigenetics and transcription. Cell Research is China＇s most promoted journal in the life sciences with a current Impact Factor of 3.426 （Thomson-ISI, PA, USA 2007）.展开更多
基金The National Outstanding Youth Foundation by NSF of ChinaTrans-Century Training Program Foundation for Talents by the Ministry of Education for financial support.
文摘Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of its 1H, 13C NMR and EI-MS spectral data. It could be inferred that the enzyme responsible for the biotransforma-tion reaction was a kind of extracellular and constitutive enzyme since the transformation reaction of the substrate could be carried out in cell free extracts of the fermentation broth of the Mucor spinosus.
文摘Ultrastructural changes in the sieve element and dense cells of nectariferous tissue during the development of floral nectary in Arabidopsis thaliana L. were investigated with transmission electron microscopy. Samples were prepared with high pressure freezing and freeze substitution techniques. The ultrastructure of dense cell was similar to that of sieve element at its early developmental stage. With the concurrent agglutination of chromatin in the nucleus, the abnormal location of organelles and the high density of cytoplasm, the ultrastructural characteristics in die dense cells of the nectariferous tissue and in the sieve element are matched with those of the programmed cell death in animal and plant reported in recent years. The disorganization of nucleus and most organelles in the differentiation of sieve elements and dense cells is closely associated with the transportation and modification of pre-nectar and the transference of nectar. This suggests that the cytological changes in sieve element and nectariferous tissue are closely associated with the nectary functional activities.
文摘[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.
文摘Anthers of male sterile line and its maintainer line of Zidao type rice, Yingxiang A and Yingxiang B were studied by cytological methods, such as F-KI staining, silver nitrate staining and paraffin sections, It is found that in Yingxiang A, there are some abnormalities in meiosis, represented by unequal division at the meiotic phase; the abortion of CMS line arises at the meiotic phase and is almost completed at the phase of monomicrospore, but only a tiny amount of aborted mi- crospores are observed; the abortion is complete and thorough, and none normal functional pollens are found; the morphology of aborted pollens is similar to that of typically aborted pollens; the tapetal cells still exist in anthers from the microsporocyte stage to the tetrad stage, but they are abnormally hyperplastic. However, in Yingxiang B, there are no abnormalities in meiosis at the meiotic phase; the tapetal cells exist in anthers before the tetrad stage, but they disappear completely when the pollen are mature. It is indicated that the abortion of CMS Yingxiang line mainly occurs at the meiotic phase, and is basically completed at the phase of monomicrospore. The abortion is probably caused by the abnormally hyperplastic tapetal cells. The abnormalities in tapetal cells may affect the normal development of pollens, resulting in the emergence of abortion. In addition, the abortion in Yingxiang A is complete and thorough, which is of great significance in hybrid breeding.
基金This project was supported by a grant from the National Natural Science Foundation of China (No. 03030302).
文摘Objective： To block the apoptosis of T lymphocytes induced by Fas/FasL in order to establish a method of the large-scale preparation of large amounts of tumor-specific cytoxic T-lymphocytes （CTL）. Methods： Liver cancer cells and tumor infiltrating lymphocytes （TIL） were isolated from FasL positive fresh specimens, and co-cultured. Specific CTL were activated and prepared in the presence of the co-stimulation of monoclonal antibody CD28. Then the blocking and activation of apoptosis of T lymphocytes was activated by soluble Fas receptor, which was detected by cytometry and DNA ladder test simultaneously. The apoptosis-blocking effect was compared with the control group. Furthermore, the changes of T cell proliferation and killing activity were detected by the method of ^3H thymidine incorporation and ^51Cr release test. Results： There was a significant increase in apoptosis rate in unblocking group compared with blocking group and quiescent group, with the unblocking group of 47.82%±0.13%, quiescent group of 3.76%±0.25%, and the blocking group of 8.22%±0.26% respectively （P〈0.01）. T cell-ladder appeared in unblocking group by DNA ladder test. Both the killing ability and proliferation rate of T cells were significantly increased after blocking. There was significant difference among blocking group, unblocking group and quiescent group （P〈0.01）. Conclusion： With this method we obtained large amounts of tumor-specific T lymphocytes, which was able to kill liver cancer cells effectively.
文摘Scope Cell Research is an international journal publishing peer- reviewed original articles, invited reviews, commentaries, and letters to the editor. Original articles report significant original basic research that describe novel molecular and cellular processes and events and/or address the underlying biological mechanisms. Reviews and Commentaries review or comment on current progress in any field covered by the journal. Reviews are generally invited in advance by the editors. Letters to the editor report novel findings that have an immediate major impact on current biological research with only one figure and less than 10 references.
文摘It has been 15 years since Cell Research was launched in 1990. At that time, Chinese scientists rarely published their work in non-Chinese journals, and China did not have a major English journal of its own in life sciences. In a way, Cell Research was born at the right time, in the right place, and with the right kind of people guiding its development. The rise of Cell Research as China＇s leading journal publishing peer-reviewed articles in basic life sciences has paralleled the rapid development of the Chinese economy in general and the science and technology sector in particular over the same period. The current economic trend should allow even more rapid advances in the life sciences which will ensure a healthy growth of Cell Research in years to come. Thus, we are confident that Cell Research has a brighter future.
文摘2016年4月22日，浙江大学医学院李晓明教授团队在《细胞研究》（Cell Research）上发表题为“ Loss of MeCP2 in eholinergic neurons causes part of RTr - like phenotypes via a7 receptor in hippocampus＂ （ http：//www, nature, com/cr/journal/ v26/n6/abs/cr201648a, html）的论文，报道了胆碱能系统通过调控γ-氨酪酸在瑞特综合征发病机制中发挥作用，并提出胡乙酰胆碱受体可能是治疗瑞特综合征的潜在靶点。
基金Supported by the Special Financial Program of Hebei Province,the Fund for Agricultural Science and Technology Innovation Talent Team Construction(2011055001)~~
文摘Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to the lack of systematic study. In recent years, the importance of Coix has been realized with the exploration of its effects in treating and preventing cancer. The systematic study of Coix has met a superb development opportunity with the rapid development of biotechnology especially the deep-sequencing technique. Therefore, this paper presented here the current status of origin and evolution, classification, cytology and molecular biology progress of Coix, analyzed the ploidy of Coix and the genetic relationships with Zea, Sorghum and so on from the point of cytology, and then based on the molecular biology, analyzed the important significance of DNA molecular marker, genetic mapping and gene library in the research on identification of genetic relationship between species and genetic breeding, which could provide useful information for further Coix research.
文摘Cell Research, an international peer-reviewed journal in English, publishes results of significance and originality in all disciplines of cell biology and molecular cell biology. The journal is indexed in PubMed, Index Medicus/MEDLINE, Science Citation Index, Current Contents-Life Sciences,SciSearch, ISI Web of Science, BIOSIS, CA, VINITI, ChinaInfo(CD & Web), Chinese Journal Full-text Database (CJFD) and Chinese Electronic Periodicals Service (CEPS) et al.
文摘CellResearch为中国科学院上海生命科学研究院主办，以全英文刊登国内外细胞生物学及其相关领域的原创性研究论文、综述、快报和述评的国际性期刊。已被Index Medicus，Medline，SCI（SciSearch ISI Web of Science），BIOSIS，CA，VINIT以及《中国科学引文数据库》、《万方数据库》（光盘版，网络版）、《中国英文版科技期刊数据库》、《中文科技期刊数据库》、《中国学术期刊》（光盘版，网络版）、
文摘Welcome to Cell Research Cell Research covers a broad scope in basic molecular and cell biology research, including cell growth and differentiation, signal transduction, apoptosis, stem cells, development, immunology, neurosciences, plant cell biology, chromatin modulation, epigenetics and transcription. Cell Research is China＇s most promoted journal in the life sciences with a current Impact Factor of 3.426 （Thomson-ISI, PA, USA 2007）.
