AIM： To investigate whether red wine and green tea could exert anti-H pylori or anti-VacA activity in vivo in a mouse model of experimental infection. METHODS： Ethanol-free red wine and green tea concentrates were a...AIM： To investigate whether red wine and green tea could exert anti-H pylori or anti-VacA activity in vivo in a mouse model of experimental infection. METHODS： Ethanol-free red wine and green tea concentrates were administered orally as a mixture of the two beverages to H pylori infected mice, or separately to VacA-treated mice. Gastric colonization and gastric inflammation were quantified by microbiological, histopathological, and immunohistochemical analyses.RESULTS： In H pylori-infected mice, the red wine and green tea mixture significantly prevented gastritis and limited the localization of bacteria and VacA to the surface of the gastric epithelium. Similarly, both beverages significantly prevented gastric epithelium damage in VacA-treated mice; green tea, but not red wine, also altered the VacA localization in the gastric epithelium. CONCLUSION： Red wine and green tea are able to prevent H pylori-induced gastric epithelium damage, possibly involving VacA inhibition. This observation supports the possible relevance of diet on the pathological outcome of Hpylori infection.展开更多
In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera （Ulva prolifera O. F. Miiller）, a familiar green...In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera （Ulva prolifera O. F. Miiller）, a familiar green macroalga along the coastline of China. Rapid accumulation of a large biomass of floating U. prolifera prompted research on different aspects of this species. In this study, we constructed a nonnormalized cDNA library from the thalli of U. prolifera and acquired 10072 high-quality expressed sequence tags （ESTs）. These ESTs were assembled into 3 519 nonredundant gene groups, including 1 446 clusters and 2 073 singletons. After annotation with the nr database, a large number of genes were found to be related with chloroplast and ribosomal protein, GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy. In addition, rather comprehensive carbon fixation pathways were found in U. prolifera using KEGG. Some stress-related and signal transduction-related genes were also found in this study. All the evidences displayed that U. prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation. Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum （Ulvophyceae）.展开更多
Acrochaete leptochaete, a species in Chaetophoraceae （Chlorophyta）, was observed during our recent laboratory culture of the macroalgae Chaetornorpha that was originally collected from an intertidal pool in Rongchen...Acrochaete leptochaete, a species in Chaetophoraceae （Chlorophyta）, was observed during our recent laboratory culture of the macroalgae Chaetornorpha that was originally collected from an intertidal pool in Rongcheng, Shandong Province, China. This is the first record of this species in China. Its morphology, taxonomy, and distribution were introduced and discussed in detail. Isolated culture experiments at different temperatures （9-29℃） and light intensities （36-108 μmol/m^2.s） were also carried out. The culture-based observations have extended our knowledge of growth morphology and general biology of the species.展开更多
Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chos...Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chosen among 11 C. vulgaris strains isolated from different fresh water ponds and their cell numbers and the amount of chlorophyll a, protein, lipid, cellulose and carbohydrate were examined. The main goal of the study is to investigate increasing the biochemical contents especially the protein content of C. vulgaris strains in different mediums. In the present study, cell densities were determined through cell count for five days. In parallel with cell count, their chlorophyll a content was determined. The highest cell density was observed with C. vulgaris TOH （Tourism and Hotel Management Pond） strain as 5.5 × 104 h/mL, and the chlorophyll a content as 4.3 × 102 mg/m3. The highest intracellular protein amount was determined with C. vulgaris GUH （Gazi University Rectorship Pond） （0.061 g/100 mL） and the highest lipid amount was attained with C. vulgaris UIK （Ulus Construction Well） strain as 0.019 g/100 mL. The process of increasing the intracellular protein amount in C. vulgaris GUH strain was carried out in Prat, Yagojinski and Chlorella medium. The results indicated that Chlorella medium increased the intracellular protein amount.展开更多
The importance of phytoplankton cell death is being increasingly recognized, however, there are still no published reports on this in Xiamen Bay. In this study, the proportion of dead phytoplankton cells associated wi...The importance of phytoplankton cell death is being increasingly recognized, however, there are still no published reports on this in Xiamen Bay. In this study, the proportion of dead phytoplankton cells associated with environmental factors was assessed at a station in Xiamen Bay from December 2012 to December 2013, using a cell digestion assay, which is an effective method to analyze dead/ living ceils in complex natural phytoplankton communities. The percentages of dead cells （% DC） in the total phytoplankton in summer （16%＋6%） were lower than those in winter （27%q-16%）. Six groups of phytoplankton （G1-G6） were categorized by flow cytometry. These phytoplankton communities with diverse seasonal variations in % DC had different responses to environmental constraints. The main factors affecting mortality were temperature and salinity, while nutrient concentration showed little influence on phytoplankton death. Additio^aally, our results provide evidence that chlorophyll a concentrations had an inverse relationship with total phytoplankton % DC and viable cell abundance was more meaningful than total cells to explain variations in environmental parameters （such as Chl a）. Moreover, the lowest mean % DC in total phytoplankton was 16%q-6% at our sample site, which is in a subtropical area with high water temperatures, full solar radiation, and rich nutrients. This indicates that phytoplankton cell death is a process that cannot be ignored. In summary, phytoplankton cell death is important in understanding the dynamics of phytoplankton communities and the fimctioning of subtropical ecosystems.展开更多
基金Supported partly by a CNR Target Project Biotechnology grantand a University of Padova Young Investigator grant
文摘AIM： To investigate whether red wine and green tea could exert anti-H pylori or anti-VacA activity in vivo in a mouse model of experimental infection. METHODS： Ethanol-free red wine and green tea concentrates were administered orally as a mixture of the two beverages to H pylori infected mice, or separately to VacA-treated mice. Gastric colonization and gastric inflammation were quantified by microbiological, histopathological, and immunohistochemical analyses.RESULTS： In H pylori-infected mice, the red wine and green tea mixture significantly prevented gastritis and limited the localization of bacteria and VacA to the surface of the gastric epithelium. Similarly, both beverages significantly prevented gastric epithelium damage in VacA-treated mice; green tea, but not red wine, also altered the VacA localization in the gastric epithelium. CONCLUSION： Red wine and green tea are able to prevent H pylori-induced gastric epithelium damage, possibly involving VacA inhibition. This observation supports the possible relevance of diet on the pathological outcome of Hpylori infection.
基金Supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01)the National Natural Science Foundation of China (No. 30830015)
文摘In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera （Ulva prolifera O. F. Miiller）, a familiar green macroalga along the coastline of China. Rapid accumulation of a large biomass of floating U. prolifera prompted research on different aspects of this species. In this study, we constructed a nonnormalized cDNA library from the thalli of U. prolifera and acquired 10072 high-quality expressed sequence tags （ESTs）. These ESTs were assembled into 3 519 nonredundant gene groups, including 1 446 clusters and 2 073 singletons. After annotation with the nr database, a large number of genes were found to be related with chloroplast and ribosomal protein, GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy. In addition, rather comprehensive carbon fixation pathways were found in U. prolifera using KEGG. Some stress-related and signal transduction-related genes were also found in this study. All the evidences displayed that U. prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation. Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum （Ulvophyceae）.
基金Supported by the National Natural Science Foundation of China(Nos.40876081,30570125)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-YW-Z-018)
文摘Acrochaete leptochaete, a species in Chaetophoraceae （Chlorophyta）, was observed during our recent laboratory culture of the macroalgae Chaetornorpha that was originally collected from an intertidal pool in Rongcheng, Shandong Province, China. This is the first record of this species in China. Its morphology, taxonomy, and distribution were introduced and discussed in detail. Isolated culture experiments at different temperatures （9-29℃） and light intensities （36-108 μmol/m^2.s） were also carried out. The culture-based observations have extended our knowledge of growth morphology and general biology of the species.
文摘Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chosen among 11 C. vulgaris strains isolated from different fresh water ponds and their cell numbers and the amount of chlorophyll a, protein, lipid, cellulose and carbohydrate were examined. The main goal of the study is to investigate increasing the biochemical contents especially the protein content of C. vulgaris strains in different mediums. In the present study, cell densities were determined through cell count for five days. In parallel with cell count, their chlorophyll a content was determined. The highest cell density was observed with C. vulgaris TOH （Tourism and Hotel Management Pond） strain as 5.5 × 104 h/mL, and the chlorophyll a content as 4.3 × 102 mg/m3. The highest intracellular protein amount was determined with C. vulgaris GUH （Gazi University Rectorship Pond） （0.061 g/100 mL） and the highest lipid amount was attained with C. vulgaris UIK （Ulus Construction Well） strain as 0.019 g/100 mL. The process of increasing the intracellular protein amount in C. vulgaris GUH strain was carried out in Prat, Yagojinski and Chlorella medium. The results indicated that Chlorella medium increased the intracellular protein amount.
基金Supported by the National Natural Science Foundation of China(Nos.41330961,41406143)the Chinese Academy of Sciences Special Pilot Program(No.XDA10020103)+1 种基金the SOA Ocean Research Project,China(No.201105021-03)the Specialized Research Fund for the Doctoral Program of Higher Education(No.20130121110031)
文摘The importance of phytoplankton cell death is being increasingly recognized, however, there are still no published reports on this in Xiamen Bay. In this study, the proportion of dead phytoplankton cells associated with environmental factors was assessed at a station in Xiamen Bay from December 2012 to December 2013, using a cell digestion assay, which is an effective method to analyze dead/ living ceils in complex natural phytoplankton communities. The percentages of dead cells （% DC） in the total phytoplankton in summer （16%＋6%） were lower than those in winter （27%q-16%）. Six groups of phytoplankton （G1-G6） were categorized by flow cytometry. These phytoplankton communities with diverse seasonal variations in % DC had different responses to environmental constraints. The main factors affecting mortality were temperature and salinity, while nutrient concentration showed little influence on phytoplankton death. Additio^aally, our results provide evidence that chlorophyll a concentrations had an inverse relationship with total phytoplankton % DC and viable cell abundance was more meaningful than total cells to explain variations in environmental parameters （such as Chl a）. Moreover, the lowest mean % DC in total phytoplankton was 16%q-6% at our sample site, which is in a subtropical area with high water temperatures, full solar radiation, and rich nutrients. This indicates that phytoplankton cell death is a process that cannot be ignored. In summary, phytoplankton cell death is important in understanding the dynamics of phytoplankton communities and the fimctioning of subtropical ecosystems.
