A method of gradient-elution HPLC with UV detection was developed for theanalysis of nine major constituents of Salvia species, a commonly used TCM herb, namely danshensu,protocatechuic acid, protocatechualdehyde, sal...A method of gradient-elution HPLC with UV detection was developed for theanalysis of nine major constituents of Salvia species, a commonly used TCM herb, namely danshensu,protocatechuic acid, protocatechualdehyde, salviano-lic acid B, methyltanshinone, dihydrotanshinone,cryptotanshinone, tanshinoneⅠand tanshinoneⅡ_A. In the present study, a Shimadzu CLC-ODS column(150 mm x 6 mm, 5 μm) was utilized and 0.5% formic acid (A) and acetonitrile (B) were used forgradient elution at a total flow rate of 0.8 mL· min^(-1). All calibration curves showed goodlinear regression ( r > 0.999) within test ranges. Extraction was conducted by refluxing methanol(10 mL) with dried herb (0.5 g) for 1.0 h.The assay was simple, convenient and reproducible. Theproposed method was successfully applied to the determination of nine major constituents in thirteenSalvia. species and the results showed that the contents of Salvia components vary in differentspecies and origin. Tanshinone was hardly detected in S. yunnanensis and S. prionitis, thereforethey are not suitable for clinical use as Danshen.展开更多
The vertical slab polyacrylamide gel electrophoretic technique is used to examine the esterase isozyme of four species (Hemiptera: Coreidae), the analysis has revealed that esterase zymogram can be divided into four z...The vertical slab polyacrylamide gel electrophoretic technique is used to examine the esterase isozyme of four species (Hemiptera: Coreidae), the analysis has revealed that esterase zymogram can be divided into four zones. Esterase zymogram of intergeneric species show distinct difference, while some intrageneric common features are discovered. The clustering analysis shows that the taxonomic status of 4 species, and sex variance is bigger than individuals and is smaller than species.展开更多
In this paper we discuss a step further some convergence and continuity problems of distribution function on R^i. We give the following results: (1)distribution function F(x_1,…,x_k) on R^k is continuous if and only ...In this paper we discuss a step further some convergence and continuity problems of distribution function on R^i. We give the following results: (1)distribution function F(x_1,…,x_k) on R^k is continuous if and only if all marginal distribution functions of F is continuous on R^1. (2)If limF_n(x_1,……,x_k)=F(x_1,…,x_k) and limF_n(x_1—0,…,x_k—0)=F(x_1—0,…,x_k—0) at all non-continuity points of F, then展开更多
Water relations of Alhagi sparsifolia Shap. at the transition zone between oases and sandy desert were studied in the southern fringe of Taklamakan Desert. Results showed that A. sparsifolia maintained the positive tu...Water relations of Alhagi sparsifolia Shap. at the transition zone between oases and sandy desert were studied in the southern fringe of Taklamakan Desert. Results showed that A. sparsifolia maintained the positive turgor during the summer. The steady high predawn water potential (psi(P)) indicated A. sparsifolia had sufficient hydration and water supply in growing season. In July, water deficit caused by drought stress had no effect on the transpiration of A. sparsifolia. Therefore, drought stress is not a main factor affecting the survival of plants. The physiological adaptation to drought of A. sparsifolia was shown mainly at the leaf level by significant difference (DeltaII) and relative water content (RWC) between the osmotic pressure at full turgor and at turgor loss, by occurring of osmotic adjustment, by high percentage of dry mass-related water content ( WCsat), by RWC at turgor loss point ( RWCp) in stable level and low RWC of the saturated symplast (RWCsym). However, the morphological features of transpiring surface reduction and deep root system seem to be the main way for the plant to adapt to the extreme drought environment. Result also suggests that one time of irregular irrigation in summer will not be helpful to recover water status of A. sparsifolia in location where the water table is very low.展开更多
Based upon the maximum entropy theorem of information theory, a novel fuzzy approach for edge detection is presented. Firstly, a definition of fuzzy partition entropy is proposed after introducing the concepts of fu...Based upon the maximum entropy theorem of information theory, a novel fuzzy approach for edge detection is presented. Firstly, a definition of fuzzy partition entropy is proposed after introducing the concepts of fuzzy probability and fuzzy partition. The relation of the probability partition and the fuzzy c-partition of the image gradient are used in the algorithm. Secondly, based on the conditional probabilities and the fuzzy partition, the optimal thresholding is searched adaptively through the maximum fuzzy entropy principle, and then the edge image is obtained. Lastly, an edge-enhancing procedure is executed on the edge image. The experimental results show that the proposed approach performs well.展开更多
The inference of genome ancestry and the estimation of molecular relatedness are of great importance for breeding efficiency and association studies. Seventy SSR loci, evenly distributed in 10 chromosomes, were assaye...The inference of genome ancestry and the estimation of molecular relatedness are of great importance for breeding efficiency and association studies. Seventy SSR loci, evenly distributed in 10 chromosomes, were assayed for polymorphism among 187 commonly used maize （Zea mays L.） inbreds which represent the genetic diversity in China. The identified 290 alleles served as raw data for estimating population structure using the coalescent linked loci, based on the ADMIXTURE model. Population number, K, has been inferred to be between five and seven. Specifying five subpopulations （K = 5） led to a distinct decrease and specifying K to be greater than six resulted in only minimal increases in the likelihood value. Therefore, population number, K, has been inferred into six subpopulations, which are PA, BSSS （includes Reid）, PB, Lan （Lancaster Sure Crop）, LRC （Luda Reb Cob, a Chinese landrace, and its derivatives）, and SPT （Si-ping-tou, a Chinese landrace and its derivatives）. The Kullback-Leibler distance of pairwise subpopulation was also inferred as n × p （187 ×6） Q matrices, which gave a detailed percentage of genetic composition of six subpopulations and molecular relatedness of each line. The genome-wide linkage disequilibrium （LD） indicated that the asso- ciation studies in QTLs and/or candidate genes might avoid nonfunctional and spurious associations, as most of the LD blocks were broken among diverse germplasm. The defined population structure has given us a clear genetic structure of these lines for breeding practice and established a good basis for association analysis.展开更多
Partially-depleted silicon-on-insulator（PDSOI）floating-body（FB）nMOSFETs and H-gate type body-contacted（BC）nMOSFETs are fabricated with different back channel implantation dosages. The off-state breakdown charact...Partially-depleted silicon-on-insulator（PDSOI）floating-body（FB）nMOSFETs and H-gate type body-contacted（BC）nMOSFETs are fabricated with different back channel implantation dosages. The off-state breakdown characteristics of these devices are presented. The off-state breakdown voltages of the FB nMOSFETs increase from 5.2 to 6. 7V, and those of the H-gate type BC nMOSFETs decrease from 11.9 to 9V as the back channel implantation dosages increase from 1.0 ×10^13 to 1.3×10^13 cm^-2. By measuring the parasitic bipolar transistor static gain and the breakdown characteristics of the pn junction between the drain and the body, the differences between the breakdown mechanisms of the FB and H-gate type BC nMOSFETs are analyzed and explained qualitatively.展开更多
In order to increase the fatigue life (FL) of road wheels (RW), a kind of double layer rubber flange (DLRF) is put forward. It consists of two layers of rubber, where metal wires are laid in the inner layer and the ou...In order to increase the fatigue life (FL) of road wheels (RW), a kind of double layer rubber flange (DLRF) is put forward. It consists of two layers of rubber, where metal wires are laid in the inner layer and the outer layer has no inlaid metal wires. Stress, strain and temperature field of DLRF were calculated with ANSYS finite element analysis (FEA) software, FL of DLRF RW was also computed with fracture mechanics fatigue theory. The results of computation indicate that the heat generated in RW's rubber flange (RF) can be reduced by the use of DLRF, and the FL of RW can be increased without affecting the mechanical intensity of RW.展开更多
[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L（safflower） varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] Th...[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L（safflower） varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] The genomic DNA of five safflower varieties from Xinjiang and one from Yunnan were analyzed and compared by SRAP molecular marker. [Result] Twelve pairs of SRAP primers were selected to amplify the genomic DNA of the six materials. Among the 171 clear DNA bands finally obtained, 93 were polymorphic, accounting for 54.4% of total. Genetic similarity coefficient of the six safflower varieties ranged from 0.60 to 0.92. [Conclusion]SRAP molecular marker is suitable for safflower varieties research and to guide the molecular breeding.展开更多
Point-of-interest(POI) recommendation is a popular topic on location-based social networks(LBSNs).Geographical proximity,known as a unique feature of LBSNs,significantly affects user check-in behavior.However,most of ...Point-of-interest(POI) recommendation is a popular topic on location-based social networks(LBSNs).Geographical proximity,known as a unique feature of LBSNs,significantly affects user check-in behavior.However,most of prior studies characterize the geographical influence based on a universal or personalized distribution of geographic distance,leading to unsatisfactory recommendation results.In this paper,the personalized geographical influence in a two-dimensional geographical space is modeled using the data field method,and we propose a semi-supervised probabilistic model based on a factor graph model to integrate different factors such as the geographical influence.Moreover,a distributed learning algorithm is used to scale up our method to large-scale data sets.Experimental results based on the data sets from Foursquare and Gowalla show that our method outperforms other competing POI recommendation techniques.展开更多
文摘A method of gradient-elution HPLC with UV detection was developed for theanalysis of nine major constituents of Salvia species, a commonly used TCM herb, namely danshensu,protocatechuic acid, protocatechualdehyde, salviano-lic acid B, methyltanshinone, dihydrotanshinone,cryptotanshinone, tanshinoneⅠand tanshinoneⅡ_A. In the present study, a Shimadzu CLC-ODS column(150 mm x 6 mm, 5 μm) was utilized and 0.5% formic acid (A) and acetonitrile (B) were used forgradient elution at a total flow rate of 0.8 mL· min^(-1). All calibration curves showed goodlinear regression ( r > 0.999) within test ranges. Extraction was conducted by refluxing methanol(10 mL) with dried herb (0.5 g) for 1.0 h.The assay was simple, convenient and reproducible. Theproposed method was successfully applied to the determination of nine major constituents in thirteenSalvia. species and the results showed that the contents of Salvia components vary in differentspecies and origin. Tanshinone was hardly detected in S. yunnanensis and S. prionitis, thereforethey are not suitable for clinical use as Danshen.
文摘The vertical slab polyacrylamide gel electrophoretic technique is used to examine the esterase isozyme of four species (Hemiptera: Coreidae), the analysis has revealed that esterase zymogram can be divided into four zones. Esterase zymogram of intergeneric species show distinct difference, while some intrageneric common features are discovered. The clustering analysis shows that the taxonomic status of 4 species, and sex variance is bigger than individuals and is smaller than species.
文摘In this paper we discuss a step further some convergence and continuity problems of distribution function on R^i. We give the following results: (1)distribution function F(x_1,…,x_k) on R^k is continuous if and only if all marginal distribution functions of F is continuous on R^1. (2)If limF_n(x_1,……,x_k)=F(x_1,…,x_k) and limF_n(x_1—0,…,x_k—0)=F(x_1—0,…,x_k—0) at all non-continuity points of F, then
文摘Water relations of Alhagi sparsifolia Shap. at the transition zone between oases and sandy desert were studied in the southern fringe of Taklamakan Desert. Results showed that A. sparsifolia maintained the positive turgor during the summer. The steady high predawn water potential (psi(P)) indicated A. sparsifolia had sufficient hydration and water supply in growing season. In July, water deficit caused by drought stress had no effect on the transpiration of A. sparsifolia. Therefore, drought stress is not a main factor affecting the survival of plants. The physiological adaptation to drought of A. sparsifolia was shown mainly at the leaf level by significant difference (DeltaII) and relative water content (RWC) between the osmotic pressure at full turgor and at turgor loss, by occurring of osmotic adjustment, by high percentage of dry mass-related water content ( WCsat), by RWC at turgor loss point ( RWCp) in stable level and low RWC of the saturated symplast (RWCsym). However, the morphological features of transpiring surface reduction and deep root system seem to be the main way for the plant to adapt to the extreme drought environment. Result also suggests that one time of irregular irrigation in summer will not be helpful to recover water status of A. sparsifolia in location where the water table is very low.
文摘Based upon the maximum entropy theorem of information theory, a novel fuzzy approach for edge detection is presented. Firstly, a definition of fuzzy partition entropy is proposed after introducing the concepts of fuzzy probability and fuzzy partition. The relation of the probability partition and the fuzzy c-partition of the image gradient are used in the algorithm. Secondly, based on the conditional probabilities and the fuzzy partition, the optimal thresholding is searched adaptively through the maximum fuzzy entropy principle, and then the edge image is obtained. Lastly, an edge-enhancing procedure is executed on the edge image. The experimental results show that the proposed approach performs well.
文摘The inference of genome ancestry and the estimation of molecular relatedness are of great importance for breeding efficiency and association studies. Seventy SSR loci, evenly distributed in 10 chromosomes, were assayed for polymorphism among 187 commonly used maize （Zea mays L.） inbreds which represent the genetic diversity in China. The identified 290 alleles served as raw data for estimating population structure using the coalescent linked loci, based on the ADMIXTURE model. Population number, K, has been inferred to be between five and seven. Specifying five subpopulations （K = 5） led to a distinct decrease and specifying K to be greater than six resulted in only minimal increases in the likelihood value. Therefore, population number, K, has been inferred into six subpopulations, which are PA, BSSS （includes Reid）, PB, Lan （Lancaster Sure Crop）, LRC （Luda Reb Cob, a Chinese landrace, and its derivatives）, and SPT （Si-ping-tou, a Chinese landrace and its derivatives）. The Kullback-Leibler distance of pairwise subpopulation was also inferred as n × p （187 ×6） Q matrices, which gave a detailed percentage of genetic composition of six subpopulations and molecular relatedness of each line. The genome-wide linkage disequilibrium （LD） indicated that the asso- ciation studies in QTLs and/or candidate genes might avoid nonfunctional and spurious associations, as most of the LD blocks were broken among diverse germplasm. The defined population structure has given us a clear genetic structure of these lines for breeding practice and established a good basis for association analysis.
文摘Partially-depleted silicon-on-insulator（PDSOI）floating-body（FB）nMOSFETs and H-gate type body-contacted（BC）nMOSFETs are fabricated with different back channel implantation dosages. The off-state breakdown characteristics of these devices are presented. The off-state breakdown voltages of the FB nMOSFETs increase from 5.2 to 6. 7V, and those of the H-gate type BC nMOSFETs decrease from 11.9 to 9V as the back channel implantation dosages increase from 1.0 ×10^13 to 1.3×10^13 cm^-2. By measuring the parasitic bipolar transistor static gain and the breakdown characteristics of the pn junction between the drain and the body, the differences between the breakdown mechanisms of the FB and H-gate type BC nMOSFETs are analyzed and explained qualitatively.
文摘In order to increase the fatigue life (FL) of road wheels (RW), a kind of double layer rubber flange (DLRF) is put forward. It consists of two layers of rubber, where metal wires are laid in the inner layer and the outer layer has no inlaid metal wires. Stress, strain and temperature field of DLRF were calculated with ANSYS finite element analysis (FEA) software, FL of DLRF RW was also computed with fracture mechanics fatigue theory. The results of computation indicate that the heat generated in RW's rubber flange (RF) can be reduced by the use of DLRF, and the FL of RW can be increased without affecting the mechanical intensity of RW.
基金Supported by Natural Science Foundation of Hubei Province(2010CDZ011)National Project for College Students to Start Innovative Career(GCX12014)~~
文摘[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L（safflower） varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] The genomic DNA of five safflower varieties from Xinjiang and one from Yunnan were analyzed and compared by SRAP molecular marker. [Result] Twelve pairs of SRAP primers were selected to amplify the genomic DNA of the six materials. Among the 171 clear DNA bands finally obtained, 93 were polymorphic, accounting for 54.4% of total. Genetic similarity coefficient of the six safflower varieties ranged from 0.60 to 0.92. [Conclusion]SRAP molecular marker is suitable for safflower varieties research and to guide the molecular breeding.
基金supported by National Key Basic Research Program of China(973 Program) under Grant No.2014CB340404National Natural Science Foundation of China under Grant Nos.61272111 and 61273216Youth Chenguang Project of Science and Technology of Wuhan City under Grant No. 2014070404010232
文摘Point-of-interest(POI) recommendation is a popular topic on location-based social networks(LBSNs).Geographical proximity,known as a unique feature of LBSNs,significantly affects user check-in behavior.However,most of prior studies characterize the geographical influence based on a universal or personalized distribution of geographic distance,leading to unsatisfactory recommendation results.In this paper,the personalized geographical influence in a two-dimensional geographical space is modeled using the data field method,and we propose a semi-supervised probabilistic model based on a factor graph model to integrate different factors such as the geographical influence.Moreover,a distributed learning algorithm is used to scale up our method to large-scale data sets.Experimental results based on the data sets from Foursquare and Gowalla show that our method outperforms other competing POI recommendation techniques.
