Spatial transcriptomics is an organizational study done on tissue sections that preserves the spatial information of the sample.Spatial transcriptomics aims to combine spatial information with gene expression data to ...Spatial transcriptomics is an organizational study done on tissue sections that preserves the spatial information of the sample.Spatial transcriptomics aims to combine spatial information with gene expression data to quantify the mRNA expression of a large number of genes in the spatial context of tissues and cells.As a paradigm shift in biological research,spatial transcriptomics can provide both spatial location information and transcriptome-level cellular gene expression data,elucidating the interactions between cells and the microenvironment.From the understanding of the entire functional life cycle of RNA to the characterization of molecular mechanisms to the mapping of gene expression in various tissue regions,by choosing the appropriate spatial transcriptome technology,researchers can achieve a deeper exploration of biological developmental processes,disease pathogenesis,etc.In recent years,the field of spatial transcriptomics has ushered in several challenges along with its rapid development,such as the dependence on sample types,the resolution of visualized genes,the difficulty of commercialization,and the ability to obtain detailed single-cell information.In this paper,we summarize and review the four major categories of spatial transcriptome technologies and compare and analyze the technical advantages and major challenges of multiple research strategies to assist current experimental design and research analysis.Finally,the importance of spatial transcriptomics in the integration of multi-omics analysis and disease modeling as well as the future development prospects are summarized and outlined.展开更多
In this study,the effect of inclination angles relative to the building direction in the additively manufactured eutectic Al-5Mg-2Si alloy was investigated through the laser powder bed fusion(LPBF).The microstructures...In this study,the effect of inclination angles relative to the building direction in the additively manufactured eutectic Al-5Mg-2Si alloy was investigated through the laser powder bed fusion(LPBF).The microstructures and mechanical properties of the Al-5Mg-2Si alloy manufactured with different inclination angles(0°,30°,45°,60°and 90°)were reported and discussed.It is found that the“semicircular”melt pool(MP)in the load bearing face of 0°sample was eventually transformed into“stripe-like”MP in the 90°sample,accompanied by an increased fraction of melt pool boundaries(MPBs).Moreover,the microstructural analysis revealed that the columnar-to-equiaxed transition(CET)of theα-Al grains and eutectic Mg2Si was completed in the 90°sample,which were significantly refined with the average size of 10.6μm and 0.44μm,respectively.It is also found that the 90°sample exhibited good combination of strength and elongation(i.e.yield strength of 393 MPa,ultimate tensile strength of 483 MPa and elongation of 8.1%).The anisotropic mechanical properties were highly associated with the refined microstructures,thermal stress,and density of MPBs.Additionally,the CET driven by inclination angles was attributed to the variation of thermal conditions inside the local MPs.展开更多
The COVID-19 pandemic encouraged the use of plastic-based personal protective equipment (PPE), which aidedgreatly in its management. However, the increased production and usage of these PPEs put a strain on the enviro...The COVID-19 pandemic encouraged the use of plastic-based personal protective equipment (PPE), which aidedgreatly in its management. However, the increased production and usage of these PPEs put a strain on the environment,especially in developing and underdeveloped countries. This has led various researchers to study low-costand effective technologies for the recycling of these materials. One such material is disposable facemasks. However,previous studies have only been able to engage electrically powered reactors for their thermochemical conversion,which is a challenge as these reactors cannot be used in regions with an insufficient supply of electricity. In thisstudy, the authors utilized a biomass-powered reactor for the conversion of waste disposable facemasks and almondleaves into hybrid biochar. The reactor, which is relatively cheap, simple to use, environmentally friendly, and modifiedfor biochar production, is biomass-powered. The co-carbonization process, which lasted 100 min, produced a 46%biochar yield, which is higher than previously obtained biochar yields by other researchers. The biochar thus obtainedwas characterized to determine its properties. FTIR analysis showed that the biochar contained functional groupssuch as alkenes, alkynes, hydroxyls, amines, and carbonyls. The EDX analysis revealed that the biochar was primarilymade of carbon, tellurium, oxygen, and calcium in the ratios of 57%, 19%, 9%, and 7%, respectively. The inclusion ofthe facemask decreased the surface area and porosity of the biochar material, as evidenced by its surface area andpore characteristics.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.22275071)
文摘Spatial transcriptomics is an organizational study done on tissue sections that preserves the spatial information of the sample.Spatial transcriptomics aims to combine spatial information with gene expression data to quantify the mRNA expression of a large number of genes in the spatial context of tissues and cells.As a paradigm shift in biological research,spatial transcriptomics can provide both spatial location information and transcriptome-level cellular gene expression data,elucidating the interactions between cells and the microenvironment.From the understanding of the entire functional life cycle of RNA to the characterization of molecular mechanisms to the mapping of gene expression in various tissue regions,by choosing the appropriate spatial transcriptome technology,researchers can achieve a deeper exploration of biological developmental processes,disease pathogenesis,etc.In recent years,the field of spatial transcriptomics has ushered in several challenges along with its rapid development,such as the dependence on sample types,the resolution of visualized genes,the difficulty of commercialization,and the ability to obtain detailed single-cell information.In this paper,we summarize and review the four major categories of spatial transcriptome technologies and compare and analyze the technical advantages and major challenges of multiple research strategies to assist current experimental design and research analysis.Finally,the importance of spatial transcriptomics in the integration of multi-omics analysis and disease modeling as well as the future development prospects are summarized and outlined.
基金Project(52071343)supported by the National Natural Science Foundation of China。
文摘In this study,the effect of inclination angles relative to the building direction in the additively manufactured eutectic Al-5Mg-2Si alloy was investigated through the laser powder bed fusion(LPBF).The microstructures and mechanical properties of the Al-5Mg-2Si alloy manufactured with different inclination angles(0°,30°,45°,60°and 90°)were reported and discussed.It is found that the“semicircular”melt pool(MP)in the load bearing face of 0°sample was eventually transformed into“stripe-like”MP in the 90°sample,accompanied by an increased fraction of melt pool boundaries(MPBs).Moreover,the microstructural analysis revealed that the columnar-to-equiaxed transition(CET)of theα-Al grains and eutectic Mg2Si was completed in the 90°sample,which were significantly refined with the average size of 10.6μm and 0.44μm,respectively.It is also found that the 90°sample exhibited good combination of strength and elongation(i.e.yield strength of 393 MPa,ultimate tensile strength of 483 MPa and elongation of 8.1%).The anisotropic mechanical properties were highly associated with the refined microstructures,thermal stress,and density of MPBs.Additionally,the CET driven by inclination angles was attributed to the variation of thermal conditions inside the local MPs.
文摘The COVID-19 pandemic encouraged the use of plastic-based personal protective equipment (PPE), which aidedgreatly in its management. However, the increased production and usage of these PPEs put a strain on the environment,especially in developing and underdeveloped countries. This has led various researchers to study low-costand effective technologies for the recycling of these materials. One such material is disposable facemasks. However,previous studies have only been able to engage electrically powered reactors for their thermochemical conversion,which is a challenge as these reactors cannot be used in regions with an insufficient supply of electricity. In thisstudy, the authors utilized a biomass-powered reactor for the conversion of waste disposable facemasks and almondleaves into hybrid biochar. The reactor, which is relatively cheap, simple to use, environmentally friendly, and modifiedfor biochar production, is biomass-powered. The co-carbonization process, which lasted 100 min, produced a 46%biochar yield, which is higher than previously obtained biochar yields by other researchers. The biochar thus obtainedwas characterized to determine its properties. FTIR analysis showed that the biochar contained functional groupssuch as alkenes, alkynes, hydroxyls, amines, and carbonyls. The EDX analysis revealed that the biochar was primarilymade of carbon, tellurium, oxygen, and calcium in the ratios of 57%, 19%, 9%, and 7%, respectively. The inclusion ofthe facemask decreased the surface area and porosity of the biochar material, as evidenced by its surface area andpore characteristics.
