[Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vect...[Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vector; the recombinants were digested by Nhe Ⅰ and Hind Ⅲ, and ligated into pEGFP-N1-P7.5, yielding the recombinant vector pEGFP-N1-P7.5-H; next the expression cassette EGFP-N1-P7.5-H was first released from recombinant vector pEGFP-N1-P7.5-H by double digestion of Hind Ⅲ and Nhe Ⅰ and ligated into pUC119-TK that was digested by Kpn Ⅰ, yielding the transfer vector pUC119-TK-EGFP-P7.5-H. [Result] Identification and double enzyme digestion showed that the transfer vector pUC119-TK-EGFP-P7.5-H was correctly constructed. From the transfer vector transfected BHK-21 cells which infected GTPV AV41, specific fluorescence was observed at 48th h of transfection. [Conclusion] The construction of goat poxvirus live vector laid a foundation for the live vector vaccine of PPR vaccine.展开更多
The authors applied a the combination of Continuous Wavelet Transform (CWT) and Fast Fourier Transform (FFT) methods to gamma ray well-log data from the Q3, G1 and D2 wells. This high-resolution stratigraphic study wa...The authors applied a the combination of Continuous Wavelet Transform (CWT) and Fast Fourier Transform (FFT) methods to gamma ray well-log data from the Q3, G1 and D2 wells. This high-resolution stratigraphic study was based on Milankovitch's orbital cycle theory. It was found that the CWT scale factors, ‘a,’ of 12, 24 and 60 match the ratios of the periodicities of precession, obliquity and eccentricity very well. Nine intervals of the Permo-carboniferous strata were recognized to have Milankovitch cycles in them. For example, section A of well Q3 has 29 precession cycles, 15 obliquity cycles and 7 short eccentricity cycles. The wavelengths are 2.7, 4.4 and 7.8 m for precession, obliquity and eccentricity, respectively. Important geological parameters such as the stratigraphic completeness and the accumulation rate were also estimated. These results provide basic information for further cyclostratigraphic correlation studies in the area. They are of great significance for the study of ancient and future climate change.展开更多
Salinity stress is one of the most serious factors limiting the distribution and productivity of crops and forest trees. The detrimental effects of salt on plants are a consequence of both a water deficit resulting in...Salinity stress is one of the most serious factors limiting the distribution and productivity of crops and forest trees. The detrimental effects of salt on plants are a consequence of both a water deficit resulting in osmotic stress and the effects of excess sodium ions on critical biochemical process. A novel approach to improve salt tolerance has been established by using the technology of plant genetic transformation and using loblolly pine (Pinus taeda L.) as a model plant. Mature zygotic embryos of loblolly pine were infected with Agrobacterium tumefaciens strain LBA 4404 harbouring the plasmid pBIGM which carrying the mannitol-1-phosphate dehydrogenase (Mt1D) and glucitol-6-phosphate dehydrogenase (GutD). Organogenic transgenic calli and transgenic regenerated plantlets were produced on selection medium containing 15mg/L kanamycin and confirmed by Southern blot analysis of genomic DNA. Salt tolerance assays demonstrated that the salt tolerance of transgenic calli and regenerated plantlets were increased. These results suggested that an efficient Agrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into loblolly pine has been developed and this could be useful for the future studies on engineering breeding of conifers.展开更多
The dynamic load spectrum is one of the most important basis of design and dynamic characteristics analysis of machines. But it is difficult to measure it on many occasions, especially for mining machines, due to thei...The dynamic load spectrum is one of the most important basis of design and dynamic characteristics analysis of machines. But it is difficult to measure it on many occasions, especially for mining machines, due to their bad working circumstances and high cost of measurements. For such situation, the load spectrum has to be obtained by indirect determination methods. A new method to identify the load spectrum, cepstrum analysis method, was presented in this paper. This method can be used to eliminate the filtering influence of transfer function to the response signals so that the load spectrum can be determined indirectly. The experimental and engineering actual examples indicates that this method has the advantages that the calculation is simple and the measurement is easy.展开更多
Rural off-farm employment,rapid loss of agricultural land,and advanced technology popularization had led agricultural production in Chinese typical rural areas come at a critical transformation and upgrade stage.Based...Rural off-farm employment,rapid loss of agricultural land,and advanced technology popularization had led agricultural production in Chinese typical rural areas come at a critical transformation and upgrade stage.Based on sample survey data gathered from 401 households in Yucheng City,Shandong Province,this paper focuses on the characteristics and mechanism of agricultural transformation in typical rural areas of the eastern China.It was found that 74.3% of households surveyed kept at least one member engaging in off-farm employment,and 57.1% looked forward to expanding the operational scale of arable land,while just 13.7% had actually transferred their agricultural land.Since growing off-farm employment does not result in the collapse of traditional subsistence agriculture,therefore,agricultural production in Yucheng City is at an incomplete transformation phase from traditional small-holder farming to modern scale economy.It is caused by both macro environments and family characteristics,while urban-rural dual structure,imperfect rural development policy and agricultural technology popularization make peasant economy strengthen upon maintenance,and small-holder farming becomes peasant's natural response owing to pluriactivity,local off-farm employment,reverse elimination,etc.In order to achieve smooth agricultural transformation of typical rural areas in the eastern China,it needs to promote professional differentiation among peasants,under clear policies adopted by central government.Providing comprehensive services for agricultural production and improving production skills and knowledge of farmers who are still living in the countryside will effectively stimulate the process of agricultural transformation.展开更多
Ridge culture is a special conservation tillage method, but the long-term influence of this tillage system on soil aggregate-size stability in paddy fields is largely unknown in southwest of China. The paper is to eva...Ridge culture is a special conservation tillage method, but the long-term influence of this tillage system on soil aggregate-size stability in paddy fields is largely unknown in southwest of China. The paper is to evaluate soil aggregate stability and to determine the relationship between soil organic carbon (SOC) and soil aggregate stability. Soil samples at 0 cm-20 cm layer were adopted from a long-term (16 a) field experiment including conventional tillage: plain culture, summer rice crop and winter upland crop under drained conditions (PUR-r); and conservation tillage: ridge culture without tillage, summer rice and winter fallow with floodwater layer annually (NTR-f), winter upland crop under drained conditions (NTR-r), and wide ridge culture without tillage, summer rice crop and winter upland crop under conditions (NTRw-r), respectively. Different pretreatments, such as slaking in fast wetting, wetting and subsequent slaking, were applied to simulate the breakdown mechanisms of aggregates in paddy soil. The results show that soil particles contents were mainly consisted of silt (0.050 mm to 1.000 mm) in fraction of 42.9% to 51.2%, sand (0.050 mm to 0.001 mm) in fraction of 28.0% to 31.8%, and clay (<0.001 mm) in fraction of 17.9% to 25.4%. The amount of aggregate-size was greatly observed in fraction of 2.000 mm-6.720 mm under ridge culture in paddy soil (more than 50$) under slaking and wetting pretreatment. The proportion of soil macro-aggregates (>0.250 mm) in conservation tillage was greatly higher than that in conventional tillage under subsequent fast slaking treatment. Minimal differences of aggregate stability between slaking in fast wetting and wetting were observed, while significant differences were found between ridge culture and plain culture. The aggregate stability under slaking treatment ranked in the order of NTR-r>NTRw-r> NTR-f > PUR-r, while under wetting was NTRw-r > NTR-r > NTR-f >PUR-r. There was a positive correlation between the aggregate stability and SOC concentration under wetting, and a low correlation was observed under slaking pretreatment. Soil exposure with tillage and lack of rice/rape-seed stubble inputs caused declines in aggregation and organic carbon, both of which make soil susceptible to water erosion. Adoption of ridge culture with no-tillage integrated with crop rotation and stubble mulch significantly altered soil organic concentration. It was a valuable conservation practice for soil aggregation and soil organic carbon sequestration on paddy soil.展开更多
On the basis of analysis of the present roofbolter’s structures, the pneumatic vane motor roofbolter was proposed and researched. Structure principle of MFT 80Y type pneumatic vane motor roofbolter was expounded, a n...On the basis of analysis of the present roofbolter’s structures, the pneumatic vane motor roofbolter was proposed and researched. Structure principle of MFT 80Y type pneumatic vane motor roofbolter was expounded, a new project of the rotary shearing mechanism with integrating pneumatic vane motor with epicyclic reduction gear unit was carried out, the mathematical model for optimum design of the rotary shearing mechanism was set up, and the optimum design for the rotary shearing mechanism was made by MATLAB Optimization Toolbox. Finally, performance of MFT 80 type pneumatic vane motor roofbolter made with optimum method and the on site experiment were introduced.展开更多
文摘[Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vector; the recombinants were digested by Nhe Ⅰ and Hind Ⅲ, and ligated into pEGFP-N1-P7.5, yielding the recombinant vector pEGFP-N1-P7.5-H; next the expression cassette EGFP-N1-P7.5-H was first released from recombinant vector pEGFP-N1-P7.5-H by double digestion of Hind Ⅲ and Nhe Ⅰ and ligated into pUC119-TK that was digested by Kpn Ⅰ, yielding the transfer vector pUC119-TK-EGFP-P7.5-H. [Result] Identification and double enzyme digestion showed that the transfer vector pUC119-TK-EGFP-P7.5-H was correctly constructed. From the transfer vector transfected BHK-21 cells which infected GTPV AV41, specific fluorescence was observed at 48th h of transfection. [Conclusion] The construction of goat poxvirus live vector laid a foundation for the live vector vaccine of PPR vaccine.
基金supported by the Project Sponsored by the Scientific Research Foundation for the Re-turned Overseas Chinese Scholars, State Education Ministry (2006331) National Basic Research Program of China (2003CB214608)
文摘The authors applied a the combination of Continuous Wavelet Transform (CWT) and Fast Fourier Transform (FFT) methods to gamma ray well-log data from the Q3, G1 and D2 wells. This high-resolution stratigraphic study was based on Milankovitch's orbital cycle theory. It was found that the CWT scale factors, ‘a,’ of 12, 24 and 60 match the ratios of the periodicities of precession, obliquity and eccentricity very well. Nine intervals of the Permo-carboniferous strata were recognized to have Milankovitch cycles in them. For example, section A of well Q3 has 29 precession cycles, 15 obliquity cycles and 7 short eccentricity cycles. The wavelengths are 2.7, 4.4 and 7.8 m for precession, obliquity and eccentricity, respectively. Important geological parameters such as the stratigraphic completeness and the accumulation rate were also estimated. These results provide basic information for further cyclostratigraphic correlation studies in the area. They are of great significance for the study of ancient and future climate change.
文摘Salinity stress is one of the most serious factors limiting the distribution and productivity of crops and forest trees. The detrimental effects of salt on plants are a consequence of both a water deficit resulting in osmotic stress and the effects of excess sodium ions on critical biochemical process. A novel approach to improve salt tolerance has been established by using the technology of plant genetic transformation and using loblolly pine (Pinus taeda L.) as a model plant. Mature zygotic embryos of loblolly pine were infected with Agrobacterium tumefaciens strain LBA 4404 harbouring the plasmid pBIGM which carrying the mannitol-1-phosphate dehydrogenase (Mt1D) and glucitol-6-phosphate dehydrogenase (GutD). Organogenic transgenic calli and transgenic regenerated plantlets were produced on selection medium containing 15mg/L kanamycin and confirmed by Southern blot analysis of genomic DNA. Salt tolerance assays demonstrated that the salt tolerance of transgenic calli and regenerated plantlets were increased. These results suggested that an efficient Agrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into loblolly pine has been developed and this could be useful for the future studies on engineering breeding of conifers.
文摘The dynamic load spectrum is one of the most important basis of design and dynamic characteristics analysis of machines. But it is difficult to measure it on many occasions, especially for mining machines, due to their bad working circumstances and high cost of measurements. For such situation, the load spectrum has to be obtained by indirect determination methods. A new method to identify the load spectrum, cepstrum analysis method, was presented in this paper. This method can be used to eliminate the filtering influence of transfer function to the response signals so that the load spectrum can be determined indirectly. The experimental and engineering actual examples indicates that this method has the advantages that the calculation is simple and the measurement is easy.
基金Under the auspices of National Natural Science Foundation of China (No 40635029, 40771014)National Key Technologies R&D Program of China (No 2006BAB15B05)
文摘Rural off-farm employment,rapid loss of agricultural land,and advanced technology popularization had led agricultural production in Chinese typical rural areas come at a critical transformation and upgrade stage.Based on sample survey data gathered from 401 households in Yucheng City,Shandong Province,this paper focuses on the characteristics and mechanism of agricultural transformation in typical rural areas of the eastern China.It was found that 74.3% of households surveyed kept at least one member engaging in off-farm employment,and 57.1% looked forward to expanding the operational scale of arable land,while just 13.7% had actually transferred their agricultural land.Since growing off-farm employment does not result in the collapse of traditional subsistence agriculture,therefore,agricultural production in Yucheng City is at an incomplete transformation phase from traditional small-holder farming to modern scale economy.It is caused by both macro environments and family characteristics,while urban-rural dual structure,imperfect rural development policy and agricultural technology popularization make peasant economy strengthen upon maintenance,and small-holder farming becomes peasant's natural response owing to pluriactivity,local off-farm employment,reverse elimination,etc.In order to achieve smooth agricultural transformation of typical rural areas in the eastern China,it needs to promote professional differentiation among peasants,under clear policies adopted by central government.Providing comprehensive services for agricultural production and improving production skills and knowledge of farmers who are still living in the countryside will effectively stimulate the process of agricultural transformation.
基金Funded by the Key Projects of National Science & Technology Pillar Program (No.2006BAD05B0-02) Sichuan Educational Committee for Youths (No.09ZB049)
文摘Ridge culture is a special conservation tillage method, but the long-term influence of this tillage system on soil aggregate-size stability in paddy fields is largely unknown in southwest of China. The paper is to evaluate soil aggregate stability and to determine the relationship between soil organic carbon (SOC) and soil aggregate stability. Soil samples at 0 cm-20 cm layer were adopted from a long-term (16 a) field experiment including conventional tillage: plain culture, summer rice crop and winter upland crop under drained conditions (PUR-r); and conservation tillage: ridge culture without tillage, summer rice and winter fallow with floodwater layer annually (NTR-f), winter upland crop under drained conditions (NTR-r), and wide ridge culture without tillage, summer rice crop and winter upland crop under conditions (NTRw-r), respectively. Different pretreatments, such as slaking in fast wetting, wetting and subsequent slaking, were applied to simulate the breakdown mechanisms of aggregates in paddy soil. The results show that soil particles contents were mainly consisted of silt (0.050 mm to 1.000 mm) in fraction of 42.9% to 51.2%, sand (0.050 mm to 0.001 mm) in fraction of 28.0% to 31.8%, and clay (<0.001 mm) in fraction of 17.9% to 25.4%. The amount of aggregate-size was greatly observed in fraction of 2.000 mm-6.720 mm under ridge culture in paddy soil (more than 50$) under slaking and wetting pretreatment. The proportion of soil macro-aggregates (>0.250 mm) in conservation tillage was greatly higher than that in conventional tillage under subsequent fast slaking treatment. Minimal differences of aggregate stability between slaking in fast wetting and wetting were observed, while significant differences were found between ridge culture and plain culture. The aggregate stability under slaking treatment ranked in the order of NTR-r>NTRw-r> NTR-f > PUR-r, while under wetting was NTRw-r > NTR-r > NTR-f >PUR-r. There was a positive correlation between the aggregate stability and SOC concentration under wetting, and a low correlation was observed under slaking pretreatment. Soil exposure with tillage and lack of rice/rape-seed stubble inputs caused declines in aggregation and organic carbon, both of which make soil susceptible to water erosion. Adoption of ridge culture with no-tillage integrated with crop rotation and stubble mulch significantly altered soil organic concentration. It was a valuable conservation practice for soil aggregation and soil organic carbon sequestration on paddy soil.
文摘On the basis of analysis of the present roofbolter’s structures, the pneumatic vane motor roofbolter was proposed and researched. Structure principle of MFT 80Y type pneumatic vane motor roofbolter was expounded, a new project of the rotary shearing mechanism with integrating pneumatic vane motor with epicyclic reduction gear unit was carried out, the mathematical model for optimum design of the rotary shearing mechanism was set up, and the optimum design for the rotary shearing mechanism was made by MATLAB Optimization Toolbox. Finally, performance of MFT 80 type pneumatic vane motor roofbolter made with optimum method and the on site experiment were introduced.
