In order to solve the problems of color bias and visual deviation caused by inaccurate estimation of transmittance and atmospheric light in image defogging,a new algorithm based on multi-scale morphological reconstruc...In order to solve the problems of color bias and visual deviation caused by inaccurate estimation of transmittance and atmospheric light in image defogging,a new algorithm based on multi-scale morphological reconstruction with adaptive transmittance and atmospheric light correction was proposed.Firstly,the algorithm used the open operation under morphological reconstruction to replace the minimum filter operation in the dark channel,and used the morphological edge to set the scale of the open operation structure elements,and constructed a multi-scale open operation fusion dark channel.After morphological noise reduction,the exact initial transmittance was obtained.According to the relationship between brightness and saturation difference and transmittance,an adaptive transmittance correction model was fitted with Gaussian function to correct the initial transmittance of the sky fog map.Then the local atmospheric light was improved according to the image brightness information and morphology closure operation.Finally,the proposed algorithm was combined with the atmospheric scattering model to obtain an accurate fog free image.The experimental results showed that the proposed algorithm was suitable for fog image restoration under various scenes,the restoration effect was good,and the brightness was suitable.展开更多
Accurate and continuous identification of individual cattle is crucial to precision farming in recent years.It is also the prerequisite to monitor the individual feed intake and feeding time of beef cattle at medium t...Accurate and continuous identification of individual cattle is crucial to precision farming in recent years.It is also the prerequisite to monitor the individual feed intake and feeding time of beef cattle at medium to long distances over different cameras.However,beef cattle can tend to frequently move and change their feeding position during feeding.Furthermore,the great variations in their head direction and complex environments(light,occlusion,and background)can also lead to some difficulties in the recognition,particularly for the bio-similarities among individual cattle.Among them,AlignedReID++model is characterized by both global and local information for image matching.In particular,the dynamically matching local information(DMLI)algorithm has been introduced into the local branch to automatically align the horizontal local information.In this research,the AlignedReID++model was utilized and improved to achieve the better performance in cattle re-identification(ReID).Initially,triplet attention(TA)modules were integrated into the BottleNecks of ResNet50 Backbone.The feature extraction was then enhanced through cross-dimensional interactions with the minimal computational overhead.Since the TA modules in AlignedReID++baseline model increased the model size and floating point operations(FLOPs)by 0.005 M and 0.05 G,the rank-1 accuracy and mean average precision(mAP)were improved by 1.0 percentage points and 2.94 percentage points,respectively.Specifically,the rank-1 accuracies were outperformed by 0.86 percentage points and 0.12 percentage points,respectively,compared with the convolution block attention module(CBAM)and efficient channel attention(ECA)modules,although 0.94 percentage points were lower than that of squeeze-and-excitation(SE)modules.The mAP metric values were exceeded by 0.22,0.86 and 0.12 percentage points,respectively,compared with the SE,CBAM,and ECA modules.Additionally,the Cross-Entropy Loss function was replaced with the CosFace Loss function in the global branch of baseline model.CosFace Loss and Hard Triplet Loss were jointly employed to train the baseline model for the better identification on the similar individuals.AlignedReID++with CosFace Loss was outperformed the baseline model by 0.24 and 0.92 percentage points in the rank-1 accuracy and mAP,respectively,whereas,AlignedReID++with ArcFace Loss was exceeded by 0.36 and 0.56 percentage points,respectively.The improved model with the TA modules and CosFace Loss was achieved in a rank-1 accuracy of 94.42%,rank-5 accuracy of 98.78%,rank-10 accuracy of 99.34%,mAP of 63.90%,FLOPs of 5.45 G,frames per second(FPS)of 5.64,and model size of 23.78 M.The rank-1 accuracies were exceeded by 1.84,4.72,0.76 and 5.36 percentage points,respectively,compared with the baseline model,part-based convolutional baseline(PCB),multiple granularity network(MGN),and relation-aware global attention(RGA),while the mAP metrics were surpassed 6.42,5.86,4.30 and 7.38 percentage points,respectively.Meanwhile,the rank-1 accuracy was 0.98 percentage points lower than TransReID,but the mAP metric was exceeded by 3.90 percentage points.Moreover,the FLOPs of improved model were only 0.05 G larger than that of baseline model,while smaller than those of PCB,MGN,RGA,and TransReID by 0.68,6.51,25.4,and 16.55 G,respectively.The model size of improved model was 23.78 M,which was smaller than those of the baseline model,PCB,MGN,RGA,and TransReID by 0.03,2.33,45.06,14.53 and 62.85 M,respectively.The inference speed of improved model on a CPU was lower than those of PCB,MGN,and baseline model,but higher than TransReID and RGA.The t-SNE feature embedding visualization demonstrated that the global and local features were achieve in the better intra-class compactness and inter-class variability.Therefore,the improved model can be expected to effectively re-identify the beef cattle in natural environments of breeding farm,in order to monitor the individual feed intake and feeding time.展开更多
A novel process that combines squeeze casting with partial remelting to obtain AZ61 magnesium alloy with semi-solid microstructures was proposed. In this route, the squeeze casting was used to predeform the magnesium ...A novel process that combines squeeze casting with partial remelting to obtain AZ61 magnesium alloy with semi-solid microstructures was proposed. In this route, the squeeze casting was used to predeform the magnesium alloy billets to obtain small dendritic structures. During subsequent partial remelting, small dendritic structures transform into globular grains surrounded by liquid films. The results show that the squeeze casting AZ61 alloy after partial remelting produces more ideal, finer semi-solid microstructure compared with as-cast AZ61 alloy treated by the same isothermal holding conditions. Moreover, the mechanical properties of the thixoformed AZ61 alloy prepared by squeeze casting plus partial remelting are better than those of the thixoformed alloy prepared by conventional casting plus partial remelting.展开更多
Modified potential ecological risk index (MRI) was proposed based on the potential ecological risk index (RI) and risk assessment code (RAC) by modifying an index. The modified index was relevant to the chemical...Modified potential ecological risk index (MRI) was proposed based on the potential ecological risk index (RI) and risk assessment code (RAC) by modifying an index. The modified index was relevant to the chemical speciation of heavy metals. Xiawan Port, a typical region contaminated by industrial production, was selected as a case study area. The total concentrations and chemical speciation of heavy metals in sediments of Xiawan Port were analyzed. The experimental data indicate that Xiawan Port is seriously polluted by heavy metals, especially by Cd. The risks of heavy metals are evaluated by RI, RAC and MRI, respectively. The resluts of MRI show that the risks of heavy metals are in the decreasing order of Cd〉Pb〉Cu〉Zn. Comparison of results by different methods reveals that MRI integrates the characters of RI and RAC. MRI is recognized to be useful for risk managemnt of heavy metals in sediments.展开更多
The pollution characteristics of Pb, Cd, Zn, Cu and Ni in soil of lead-zinc mining area were studied. The results indicate that the contamination degree followed the sequence of Cd〉Pb〉Zn〉Ni〉Cu and concentrations o...The pollution characteristics of Pb, Cd, Zn, Cu and Ni in soil of lead-zinc mining area were studied. The results indicate that the contamination degree followed the sequence of Cd〉Pb〉Zn〉Ni〉Cu and concentrations of Pb, Cd and Zn exceeded corresponding limits of the Chinese National Soil Environmental Quality Standard III. The soil was extremely polluted by Cd(Iego=5.26), moderately to heavily polluted by Zn(Iego=2.38), heavily to extremely polluted by Pb(Iego=4.13). The results of BCR three-step sequential extraction procedure show that the active Cd, Pb and Zn were relatively high and might exert adverse effects on the plants grown in the soil, while Cu and Ni existed in soil with a relatively stable form. Potential ecological risk results indicate that soils were engaging in a high potential ecological risk by pollution of Cd and should be given rise to concern.展开更多
This paper sets up a robotic manipulator model on slewing crane. The model can synthetically describe the dynamic behavior of the load of slewing crane in rotating, elevating and hoisting motions. The dynamic equation...This paper sets up a robotic manipulator model on slewing crane. The model can synthetically describe the dynamic behavior of the load of slewing crane in rotating, elevating and hoisting motions. The dynamic equations of the system are recursively derived by a Newton Euler method. The dynamic behavior of the load of slewing crane in rotating motion is simulated on a computer. The method of robotic dynamics to derive the dynamic equations of the swing of load is accurate and convenient and it has good regularity. The result of the study provides a base in theory on design of crane and an accurate mathematical model for controlling the swing of load.展开更多
Plant development is a process from germination of seed to vegetative growth, flower initiation and development, fertilization and finally to the next generation seed formation. A lot of achievements have been obtai...Plant development is a process from germination of seed to vegetative growth, flower initiation and development, fertilization and finally to the next generation seed formation. A lot of achievements have been obtained in plant developmental biology in China. Since the 1990's, those young generation scientists returned to China from abroad to use molecular and genetic techniques to study morphological, physiological and biochemical process of plant development. The present paper reviews the progress in some research area of plant developmental biology in the past decades and also prospects the chance and future of plant developmental studies, due to the recent advances of plant genome sequencing and functional genomics in China under the international research background.展开更多
[Objective] The aim was to investigate the effects of soil geochemistry on the quality of winter jujube.[Method]Based on the data of ecological geochemical survey in lower Yellow River Basin,the relevance of the geoch...[Objective] The aim was to investigate the effects of soil geochemistry on the quality of winter jujube.[Method]Based on the data of ecological geochemical survey in lower Yellow River Basin,the relevance of the geochemistry environment in top soils and the quality of winter jujubes was analyzed.[Result]Cd,Ni and other heavy metals in the soil more obviously affected the nutrition of winter jujubes.[Conclusion]B,Mn,MgO,Cd,Zn,K2O and other elements in the root soil played an important role in the enrichment of Cr and Hg of winter jujube fruit.And the absorption of heavy metals in winter jujube root could be interfered by improving fertilization method,to reduce the enrichment of harmful elements in jujube fruit.展开更多
Laser ablated boron atoms have been reacted with hydrogen sulfide and the reaction products condensed with argon at 4 K, which gave BS2, BS2-, HSBS, and HBS molecules. Reagent isotopic substitution (H2S, H234S, D2S, ...Laser ablated boron atoms have been reacted with hydrogen sulfide and the reaction products condensed with argon at 4 K, which gave BS2, BS2-, HSBS, and HBS molecules. Reagent isotopic substitution (H2S, H234S, D2S, 10 B, 11B) and variation of boron and hydrogen sulfide concentrations have been used to identify the major products. Both BS2-- and BS2 were identified as linear molecules with no significant difference in the structure parameters, but the B-S anti-symmetric stretching vibration of BS2 is significantly lower than that of BS2-, which is ascribed to pseudo Jahn-Teller effect. Theoretical calculation was employed to have an insight into the interaction nature of the bonds in the corresponding products.展开更多
A total of 153 soil samples were collected from Changsha City, China, to analyze the contents of As, Cd, Cr, Cu, Hg, Mn, Ni, Pb and Zn. A combination of sampling data, multivariate statistical method, geostatistical a...A total of 153 soil samples were collected from Changsha City, China, to analyze the contents of As, Cd, Cr, Cu, Hg, Mn, Ni, Pb and Zn. A combination of sampling data, multivariate statistical method, geostatistical analysis, direct exposure method and triangulated irregular network (TIN) model was successfully employed to discriminate sources, simulate spatial distributions and evaluate children's health risks of heavy metals in soils. The results show that not all sites in Changsha city may be suitable for living without remediation. About 9.0% of the study area provided a hazard index (HI)1.0, and 1.9% had an HI2.0. Most high HIs were located in the southern and western areas. The element of arsenic and the pathway of soil ingestion were the largest contribution to potential health risks for children. This study indicates that we should attach great importance to the direct soil heavy metals exposure for children's health.展开更多
Objective: To investigate the relationship among the latest WHO classification of thymoma, myasthenia gravis (MG) and clinical stages. Methods: To review the pathological sections of 74 patients with thymoma from ...Objective: To investigate the relationship among the latest WHO classification of thymoma, myasthenia gravis (MG) and clinical stages. Methods: To review the pathological sections of 74 patients with thymoma from 1980-2004 using WHO classification (1999), the statistical software was used to analyze the relationship among the WHO classification, MG and clinical stages. Results: (1) Two cases of type A, 23 cases of type AB, 4 cases of type B1, 27 cases of type B2, 16 cases of type B3 and 2 cases of type C were classified. Type B2 more likely accompanied MG (P〈0.05), while none with MG occurred for type C. (2) One patient was in stage Ⅰ, 30 were in stage Ⅱ, 38 were in stage Ⅲ, and 5 were in stage Ⅳ. The latest histologic classification was significantly correlated with Masaoka stages (P〈0.01). Conclusion: The latest WHO classification was correlated with occurrence of MG and finely reflected clinical stage. It can also evaluate the prognosis of patients.展开更多
This paper aims to provide better guidance for construction of trehalose-producing recombinant strains to further improve the yield of trehalose. The research progress on trehalose biosynthesis pathways and the applic...This paper aims to provide better guidance for construction of trehalose-producing recombinant strains to further improve the yield of trehalose. The research progress on trehalose biosynthesis pathways and the application of molecular biology technique in trehalose study in recent 30 years, especially the last 10 years are reviewed. Results show that there are 5 pathways of trehalose synthesis. Although enzymes and genes of trehalose synthesis have been isolated and genetic engineering strains have increased gradually, the improvement of trehalose yield is still inadequate because most recombinant strains are limited to study the physicochemical properties of single enzyme. With the development of modern biological technology, especially the rapid development of DNA recombinant technology, metagenomics and synthetic biology, high expression of heterologous trehalose in recombinant strains would become a hot research topic in the future.展开更多
[Objective] The aim of this study was to realize efficient expression of the porcine reproductive and respiratory syndrome virus (PRRSV) ORF7 gene in genetic engineering bacteria and analYze the immunological activi...[Objective] The aim of this study was to realize efficient expression of the porcine reproductive and respiratory syndrome virus (PRRSV) ORF7 gene in genetic engineering bacteria and analYze the immunological activity of the recombinant protein after purification. [ Method] The constructed recombinant expression vector pET-ORF7 was transformed into Escherichia co1BL21 (DE3) and induced by IPTG under the optimal condition. After analysis of SDS-PAGE and Western Blot, the expression products were purified by Ni-NTA His · Bind Resin chrom- atographic column under denaturing condition and renatured by gradient dialysis. Subsequently, the immunological activity of the renatured recombinant protein was detected by Westem Blot and indirect ELISA. [ Result] The recombinant plasmid pET-ORF7 expressed in E. coli successfully, and the fusion protein was in the form of inclusion body. By SDS-PAGE detection, the molecular weight of the expression protein was approximate 33 kD, according with the expectation. Analysis by Bandscan software showed that the expressed fusion protein was about 50% of total bacterial protein of BL21 (DE3). Wastem Blot and indirect ELISA detection showed that the renatured protein could react with PRRSV positive serum specifically, indicating its good immunological activity. [ Conclusion] This study lays a foundation for the preparation of PRRSV monoclonal antibody and diagnostic kit.展开更多
The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted...The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted in urine, feces and bile within 24 h afterintravenous administration of ^(125)I recombinant E. col L-asparaginase to rats was 68.95% ,4.44%and 5.36% of the dose respectively. ^(125)I recombinant E. coli L-asparaginase in plasma samples wasdetermined. The levels of structural intact molecule in plasma samples were evaluated by SDS-PAGEand bio-imaging analyzer system. Pharmacokinetic parameters were assessed with a model-dependentmethod. The concentration-time curves of recombinant E. coli L-asparaginase after intravenousinjection at 1 250 IU·kg^(-1), 2 500, IU·kg^(-1), 5 000 IU·kg^(-1) to rats were consistent withthe two-compartment model. The first and terminal elimination t_(1/2) were 0.52 ~ 0.63 h and 2.39 ~2.76 h respectively. AUC was linearly related to the doses. The results of distribution in tissuesor organs and excretion in urine suggested that the metabolites of the enzyme were cleared bymechanisms of urinary excretion. Pharmacokinetics parameters of recombinant E. coli L- asparaginasein rats are warranted for the design of future clinical trials.展开更多
\ According to the analysis of the residual products by thermogravimetric analysis (TGA), the thermal decomposition process of cefazolin sodium (CEZ·Na) was thought to be similar to the degradation in solid sta...\ According to the analysis of the residual products by thermogravimetric analysis (TGA), the thermal decomposition process of cefazolin sodium (CEZ·Na) was thought to be similar to the degradation in solid state in its storage time. This laid a foundation for estimating the relative chemical stability of the drug by determination of its decomposition kinetics using TGA. Although the observed thermal decomposition kinetics of CEZ·Na was complex, a conversion level of 1% was chosen for evaluation of the stability of CEZ·Na crystalline since the mechanism here was more likely to be that of the actual product failure. The evaluation results suggested that the α form of CEZ·Na had the best stability and the amorphous one was the least stable one among α form, dehydrated α form and amorphous form.展开更多
Gravity die casting(GC) and squeeze casting(SC) T4-treated Al-7.0Zn-2.5Mg-2.1Cu alloys were employed to investigate the microstructures,mechanical properties and low cycle fatigue(LCF) behavior.The results show that m...Gravity die casting(GC) and squeeze casting(SC) T4-treated Al-7.0Zn-2.5Mg-2.1Cu alloys were employed to investigate the microstructures,mechanical properties and low cycle fatigue(LCF) behavior.The results show that mechanical properties of SC specimens are significantly better than those of GC specimens due to less cast defects and smaller secondary dendrite arm spacing(SDAS).Excellent fatigue properties are obtained for the SC alloy compared with the GC alloy.GC and SC alloys both exhibit cyclic stabilization at low total strain amplitudes(less than 0.4%) and cyclic hardening at higher total strain amplitudes.The degree of cyclic hardening of SC samples is greater than that of GC samples.Fatigue cracks of GC samples dominantly initiate from shrinkage porosities and are easy to propagate along them,while the crack initiation sites for SC samples are slip bands,eutectic phases and inclusions at or near the free surface.展开更多
Functionalizing and patterning of the silicon surface can be realized simultaneously by the chemomechanical method. The oxide-coated crystalline silicon (100) surface is scratched with a diamond tool in the presence...Functionalizing and patterning of the silicon surface can be realized simultaneously by the chemomechanical method. The oxide-coated crystalline silicon (100) surface is scratched with a diamond tool in the presence of aryldiazonium salt (C6H5N2BF4). Scratching activates the silicon surface by removing the passivation oxide layer to expose fresh Si atoms. The sur- face morphologies before and after chemomechanical reaction are characterized with atomic force microscopy. Time-of-flight secondary ion mass spectroscopy confirms the presence of C6H5 and provides evidence for the formation of self-assembled monolayer (SAM) on silicon surface via Si-C covalent bonds by scratching the silicon in the presence of C6H5N2BF4. C6H5 groups further bond with surface Si atoms via Si-C covalent bonds as confirmed from infrared spectroscopy results. We propose that chemomechanical reaction, which occurred during scratching the silicon surface, produce C6H5 groups from aryldiazonium salt. The relevant adhesion of SAM is measured. It is found that SAM can reduce the adhesion of silicon. The monolayer can be used as anti-adhesion monolayer for micro/nanoelectromechanical systems components under different environments and operating conditions.展开更多
To identify the main sources responsible for soil heavy metal contamination, 70 topsoils were sampled from the Daxing County in the urban-rural transition zone of Beijing. The concentrations of heavy metals Cu, Zn, Pb...To identify the main sources responsible for soil heavy metal contamination, 70 topsoils were sampled from the Daxing County in the urban-rural transition zone of Beijing. The concentrations of heavy metals Cu, Zn, Pb, Cr, Cd, Ni, As, Se, Hg, and Co; the soil texture; and the organic matter content were determined for each soil sample. Descriptive statistics and geostatistics were used to analyze the data, and Kriging analysis was used to estimate the unobserved points and to map the spatial patterns of soil heavy metals. The results showed that the concentrations of all the soil heavy metals exceeded their background levels with the exception of As and Se. However, only the Cd concentration in some areas exceeded the critical value of the national soil quality standard. The semivariance analysis showed that the spatial correlation distances for soil Cu, Zn, Cr, Cd, As, Ni, and Co ranged from 4.0 to 7.0 km, but soil Se, Pb, and Hg had a larger correlation distance. Soil Co, Se, Cd, Cu and Zn showed a strong spatial correlation, whereas the other soil heavy metals showed medium spatial correlation. Soil heavy metal concentrations were related to soil texture, organic matter content, and the accumulation of heavy metals in the soils, which was because of air deposition and use of water from the Liangshui, Xinfeng, and Fenghe rivers that are contaminated by wastewater and sewage for the purpose of irrigation of fields. Hence, a comprehensive treatment plan for these rivers should be formulated.展开更多
基金supported by National Natural Science Foundation of China(No.61561030)College Industry Support Plan Project of Gansu Provincial Department of Education(No.2021CYZC-04)Educational Reform Fund of Lanzhou Jiaotong University(No.JG201928)。
文摘In order to solve the problems of color bias and visual deviation caused by inaccurate estimation of transmittance and atmospheric light in image defogging,a new algorithm based on multi-scale morphological reconstruction with adaptive transmittance and atmospheric light correction was proposed.Firstly,the algorithm used the open operation under morphological reconstruction to replace the minimum filter operation in the dark channel,and used the morphological edge to set the scale of the open operation structure elements,and constructed a multi-scale open operation fusion dark channel.After morphological noise reduction,the exact initial transmittance was obtained.According to the relationship between brightness and saturation difference and transmittance,an adaptive transmittance correction model was fitted with Gaussian function to correct the initial transmittance of the sky fog map.Then the local atmospheric light was improved according to the image brightness information and morphology closure operation.Finally,the proposed algorithm was combined with the atmospheric scattering model to obtain an accurate fog free image.The experimental results showed that the proposed algorithm was suitable for fog image restoration under various scenes,the restoration effect was good,and the brightness was suitable.
基金National Key Research and Development Program(2023YFD1301801)National Natural Science Foundation of China(32272931)+1 种基金Shaanxi Province Agricultural Key Core Technology Project(2024NYGG005)Shaanxi Province Key R&D Program(2024NC-ZDCYL-05-12)。
文摘Accurate and continuous identification of individual cattle is crucial to precision farming in recent years.It is also the prerequisite to monitor the individual feed intake and feeding time of beef cattle at medium to long distances over different cameras.However,beef cattle can tend to frequently move and change their feeding position during feeding.Furthermore,the great variations in their head direction and complex environments(light,occlusion,and background)can also lead to some difficulties in the recognition,particularly for the bio-similarities among individual cattle.Among them,AlignedReID++model is characterized by both global and local information for image matching.In particular,the dynamically matching local information(DMLI)algorithm has been introduced into the local branch to automatically align the horizontal local information.In this research,the AlignedReID++model was utilized and improved to achieve the better performance in cattle re-identification(ReID).Initially,triplet attention(TA)modules were integrated into the BottleNecks of ResNet50 Backbone.The feature extraction was then enhanced through cross-dimensional interactions with the minimal computational overhead.Since the TA modules in AlignedReID++baseline model increased the model size and floating point operations(FLOPs)by 0.005 M and 0.05 G,the rank-1 accuracy and mean average precision(mAP)were improved by 1.0 percentage points and 2.94 percentage points,respectively.Specifically,the rank-1 accuracies were outperformed by 0.86 percentage points and 0.12 percentage points,respectively,compared with the convolution block attention module(CBAM)and efficient channel attention(ECA)modules,although 0.94 percentage points were lower than that of squeeze-and-excitation(SE)modules.The mAP metric values were exceeded by 0.22,0.86 and 0.12 percentage points,respectively,compared with the SE,CBAM,and ECA modules.Additionally,the Cross-Entropy Loss function was replaced with the CosFace Loss function in the global branch of baseline model.CosFace Loss and Hard Triplet Loss were jointly employed to train the baseline model for the better identification on the similar individuals.AlignedReID++with CosFace Loss was outperformed the baseline model by 0.24 and 0.92 percentage points in the rank-1 accuracy and mAP,respectively,whereas,AlignedReID++with ArcFace Loss was exceeded by 0.36 and 0.56 percentage points,respectively.The improved model with the TA modules and CosFace Loss was achieved in a rank-1 accuracy of 94.42%,rank-5 accuracy of 98.78%,rank-10 accuracy of 99.34%,mAP of 63.90%,FLOPs of 5.45 G,frames per second(FPS)of 5.64,and model size of 23.78 M.The rank-1 accuracies were exceeded by 1.84,4.72,0.76 and 5.36 percentage points,respectively,compared with the baseline model,part-based convolutional baseline(PCB),multiple granularity network(MGN),and relation-aware global attention(RGA),while the mAP metrics were surpassed 6.42,5.86,4.30 and 7.38 percentage points,respectively.Meanwhile,the rank-1 accuracy was 0.98 percentage points lower than TransReID,but the mAP metric was exceeded by 3.90 percentage points.Moreover,the FLOPs of improved model were only 0.05 G larger than that of baseline model,while smaller than those of PCB,MGN,RGA,and TransReID by 0.68,6.51,25.4,and 16.55 G,respectively.The model size of improved model was 23.78 M,which was smaller than those of the baseline model,PCB,MGN,RGA,and TransReID by 0.03,2.33,45.06,14.53 and 62.85 M,respectively.The inference speed of improved model on a CPU was lower than those of PCB,MGN,and baseline model,but higher than TransReID and RGA.The t-SNE feature embedding visualization demonstrated that the global and local features were achieve in the better intra-class compactness and inter-class variability.Therefore,the improved model can be expected to effectively re-identify the beef cattle in natural environments of breeding farm,in order to monitor the individual feed intake and feeding time.
基金Project(51405466)supported by the National Natural Science Foundation of ChinaProject(Y32Z010F10)supported by the Western Light Program of the Chinese Academy of Sciences+1 种基金Project(cstc2014jcyj A50009)supported by Chongqing Research of Application Foundation and Advanced Technology,ChinaProject(cstc2014jcyj A50037)supported by Chongqing Research of Application Foundation and Advanced Technology,China
文摘A novel process that combines squeeze casting with partial remelting to obtain AZ61 magnesium alloy with semi-solid microstructures was proposed. In this route, the squeeze casting was used to predeform the magnesium alloy billets to obtain small dendritic structures. During subsequent partial remelting, small dendritic structures transform into globular grains surrounded by liquid films. The results show that the squeeze casting AZ61 alloy after partial remelting produces more ideal, finer semi-solid microstructure compared with as-cast AZ61 alloy treated by the same isothermal holding conditions. Moreover, the mechanical properties of the thixoformed AZ61 alloy prepared by squeeze casting plus partial remelting are better than those of the thixoformed alloy prepared by conventional casting plus partial remelting.
基金Projects (51039001, 50978087, 51009063, 50808071) supported by the National Natural Science Foundation of ChinaProject (SX2010-026) supported by State Council Three Gorges Project Construction Committee Executive Office,China+2 种基金Project (2009ZX07212-001) supported by Ministry of Environmental Protection of ChinaProject (BYHGLC-2010-02) supported by Guangzhou Water Authority,ChinaProject (CX2010B157) supported by Hunan Provincial Innovation Foundation for Postgraduate,China
文摘Modified potential ecological risk index (MRI) was proposed based on the potential ecological risk index (RI) and risk assessment code (RAC) by modifying an index. The modified index was relevant to the chemical speciation of heavy metals. Xiawan Port, a typical region contaminated by industrial production, was selected as a case study area. The total concentrations and chemical speciation of heavy metals in sediments of Xiawan Port were analyzed. The experimental data indicate that Xiawan Port is seriously polluted by heavy metals, especially by Cd. The risks of heavy metals are evaluated by RI, RAC and MRI, respectively. The resluts of MRI show that the risks of heavy metals are in the decreasing order of Cd〉Pb〉Cu〉Zn. Comparison of results by different methods reveals that MRI integrates the characters of RI and RAC. MRI is recognized to be useful for risk managemnt of heavy metals in sediments.
基金Project(2012FJ1010)supported by the National Science and Technology Major Project,ChinaProject(2012BAC09B04)supported by the National"Twelfth Five-Year"Plan for Science&Technology,ChinaProject(2012AA06202)supported by the National High-tech Research and Development Program of China
文摘The pollution characteristics of Pb, Cd, Zn, Cu and Ni in soil of lead-zinc mining area were studied. The results indicate that the contamination degree followed the sequence of Cd〉Pb〉Zn〉Ni〉Cu and concentrations of Pb, Cd and Zn exceeded corresponding limits of the Chinese National Soil Environmental Quality Standard III. The soil was extremely polluted by Cd(Iego=5.26), moderately to heavily polluted by Zn(Iego=2.38), heavily to extremely polluted by Pb(Iego=4.13). The results of BCR three-step sequential extraction procedure show that the active Cd, Pb and Zn were relatively high and might exert adverse effects on the plants grown in the soil, while Cu and Ni existed in soil with a relatively stable form. Potential ecological risk results indicate that soils were engaging in a high potential ecological risk by pollution of Cd and should be given rise to concern.
文摘This paper sets up a robotic manipulator model on slewing crane. The model can synthetically describe the dynamic behavior of the load of slewing crane in rotating, elevating and hoisting motions. The dynamic equations of the system are recursively derived by a Newton Euler method. The dynamic behavior of the load of slewing crane in rotating motion is simulated on a computer. The method of robotic dynamics to derive the dynamic equations of the swing of load is accurate and convenient and it has good regularity. The result of the study provides a base in theory on design of crane and an accurate mathematical model for controlling the swing of load.
文摘Plant development is a process from germination of seed to vegetative growth, flower initiation and development, fertilization and finally to the next generation seed formation. A lot of achievements have been obtained in plant developmental biology in China. Since the 1990's, those young generation scientists returned to China from abroad to use molecular and genetic techniques to study morphological, physiological and biochemical process of plant development. The present paper reviews the progress in some research area of plant developmental biology in the past decades and also prospects the chance and future of plant developmental studies, due to the recent advances of plant genome sequencing and functional genomics in China under the international research background.
基金Supported by China Geological Survey Project(121210310306)~~
文摘[Objective] The aim was to investigate the effects of soil geochemistry on the quality of winter jujube.[Method]Based on the data of ecological geochemical survey in lower Yellow River Basin,the relevance of the geochemistry environment in top soils and the quality of winter jujubes was analyzed.[Result]Cd,Ni and other heavy metals in the soil more obviously affected the nutrition of winter jujubes.[Conclusion]B,Mn,MgO,Cd,Zn,K2O and other elements in the root soil played an important role in the enrichment of Cr and Hg of winter jujube fruit.And the absorption of heavy metals in winter jujube root could be interfered by improving fertilization method,to reduce the enrichment of harmful elements in jujube fruit.
文摘Laser ablated boron atoms have been reacted with hydrogen sulfide and the reaction products condensed with argon at 4 K, which gave BS2, BS2-, HSBS, and HBS molecules. Reagent isotopic substitution (H2S, H234S, D2S, 10 B, 11B) and variation of boron and hydrogen sulfide concentrations have been used to identify the major products. Both BS2-- and BS2 were identified as linear molecules with no significant difference in the structure parameters, but the B-S anti-symmetric stretching vibration of BS2 is significantly lower than that of BS2-, which is ascribed to pseudo Jahn-Teller effect. Theoretical calculation was employed to have an insight into the interaction nature of the bonds in the corresponding products.
基金Project (50925417) supported by the National Funds for Distinguished Young Scientists, ChinaProject (50830301) supported by the Key Project of National Natural Science Foundation of China
文摘A total of 153 soil samples were collected from Changsha City, China, to analyze the contents of As, Cd, Cr, Cu, Hg, Mn, Ni, Pb and Zn. A combination of sampling data, multivariate statistical method, geostatistical analysis, direct exposure method and triangulated irregular network (TIN) model was successfully employed to discriminate sources, simulate spatial distributions and evaluate children's health risks of heavy metals in soils. The results show that not all sites in Changsha city may be suitable for living without remediation. About 9.0% of the study area provided a hazard index (HI)1.0, and 1.9% had an HI2.0. Most high HIs were located in the southern and western areas. The element of arsenic and the pathway of soil ingestion were the largest contribution to potential health risks for children. This study indicates that we should attach great importance to the direct soil heavy metals exposure for children's health.
文摘Objective: To investigate the relationship among the latest WHO classification of thymoma, myasthenia gravis (MG) and clinical stages. Methods: To review the pathological sections of 74 patients with thymoma from 1980-2004 using WHO classification (1999), the statistical software was used to analyze the relationship among the WHO classification, MG and clinical stages. Results: (1) Two cases of type A, 23 cases of type AB, 4 cases of type B1, 27 cases of type B2, 16 cases of type B3 and 2 cases of type C were classified. Type B2 more likely accompanied MG (P〈0.05), while none with MG occurred for type C. (2) One patient was in stage Ⅰ, 30 were in stage Ⅱ, 38 were in stage Ⅲ, and 5 were in stage Ⅳ. The latest histologic classification was significantly correlated with Masaoka stages (P〈0.01). Conclusion: The latest WHO classification was correlated with occurrence of MG and finely reflected clinical stage. It can also evaluate the prognosis of patients.
文摘This paper aims to provide better guidance for construction of trehalose-producing recombinant strains to further improve the yield of trehalose. The research progress on trehalose biosynthesis pathways and the application of molecular biology technique in trehalose study in recent 30 years, especially the last 10 years are reviewed. Results show that there are 5 pathways of trehalose synthesis. Although enzymes and genes of trehalose synthesis have been isolated and genetic engineering strains have increased gradually, the improvement of trehalose yield is still inadequate because most recombinant strains are limited to study the physicochemical properties of single enzyme. With the development of modern biological technology, especially the rapid development of DNA recombinant technology, metagenomics and synthetic biology, high expression of heterologous trehalose in recombinant strains would become a hot research topic in the future.
文摘[Objective] The aim of this study was to realize efficient expression of the porcine reproductive and respiratory syndrome virus (PRRSV) ORF7 gene in genetic engineering bacteria and analYze the immunological activity of the recombinant protein after purification. [ Method] The constructed recombinant expression vector pET-ORF7 was transformed into Escherichia co1BL21 (DE3) and induced by IPTG under the optimal condition. After analysis of SDS-PAGE and Western Blot, the expression products were purified by Ni-NTA His · Bind Resin chrom- atographic column under denaturing condition and renatured by gradient dialysis. Subsequently, the immunological activity of the renatured recombinant protein was detected by Westem Blot and indirect ELISA. [ Result] The recombinant plasmid pET-ORF7 expressed in E. coli successfully, and the fusion protein was in the form of inclusion body. By SDS-PAGE detection, the molecular weight of the expression protein was approximate 33 kD, according with the expectation. Analysis by Bandscan software showed that the expressed fusion protein was about 50% of total bacterial protein of BL21 (DE3). Wastem Blot and indirect ELISA detection showed that the renatured protein could react with PRRSV positive serum specifically, indicating its good immunological activity. [ Conclusion] This study lays a foundation for the preparation of PRRSV monoclonal antibody and diagnostic kit.
基金ProjectsupportedbytheNationalNinth FivePlanKeyProjectFoundation No 96 90 2 0 1 2 5
文摘The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted in urine, feces and bile within 24 h afterintravenous administration of ^(125)I recombinant E. col L-asparaginase to rats was 68.95% ,4.44%and 5.36% of the dose respectively. ^(125)I recombinant E. coli L-asparaginase in plasma samples wasdetermined. The levels of structural intact molecule in plasma samples were evaluated by SDS-PAGEand bio-imaging analyzer system. Pharmacokinetic parameters were assessed with a model-dependentmethod. The concentration-time curves of recombinant E. coli L-asparaginase after intravenousinjection at 1 250 IU·kg^(-1), 2 500, IU·kg^(-1), 5 000 IU·kg^(-1) to rats were consistent withthe two-compartment model. The first and terminal elimination t_(1/2) were 0.52 ~ 0.63 h and 2.39 ~2.76 h respectively. AUC was linearly related to the doses. The results of distribution in tissuesor organs and excretion in urine suggested that the metabolites of the enzyme were cleared bymechanisms of urinary excretion. Pharmacokinetics parameters of recombinant E. coli L- asparaginasein rats are warranted for the design of future clinical trials.
文摘\ According to the analysis of the residual products by thermogravimetric analysis (TGA), the thermal decomposition process of cefazolin sodium (CEZ·Na) was thought to be similar to the degradation in solid state in its storage time. This laid a foundation for estimating the relative chemical stability of the drug by determination of its decomposition kinetics using TGA. Although the observed thermal decomposition kinetics of CEZ·Na was complex, a conversion level of 1% was chosen for evaluation of the stability of CEZ·Na crystalline since the mechanism here was more likely to be that of the actual product failure. The evaluation results suggested that the α form of CEZ·Na had the best stability and the amorphous one was the least stable one among α form, dehydrated α form and amorphous form.
基金Project(2015A030312003)supported by the Guangdong Natural Science Foundation for Research Team,ChinaProject(51374110)supported by the National Natural Science Foundation of China
文摘Gravity die casting(GC) and squeeze casting(SC) T4-treated Al-7.0Zn-2.5Mg-2.1Cu alloys were employed to investigate the microstructures,mechanical properties and low cycle fatigue(LCF) behavior.The results show that mechanical properties of SC specimens are significantly better than those of GC specimens due to less cast defects and smaller secondary dendrite arm spacing(SDAS).Excellent fatigue properties are obtained for the SC alloy compared with the GC alloy.GC and SC alloys both exhibit cyclic stabilization at low total strain amplitudes(less than 0.4%) and cyclic hardening at higher total strain amplitudes.The degree of cyclic hardening of SC samples is greater than that of GC samples.Fatigue cracks of GC samples dominantly initiate from shrinkage porosities and are easy to propagate along them,while the crack initiation sites for SC samples are slip bands,eutectic phases and inclusions at or near the free surface.
基金We thank Prof. Yang Gan of Harbin Institute of Technology, and Prof. Fu-long Yuan of Heilongjiang University for the help in the experiments. This work was supported by the Center for Precision Engineering of Harbin Institute of Technology, the Youth the Colleges and Universities in Heilongjiang Province in 2010 (No.1155G54), the Training Fund Project of Jiamusi University (No.RC2009-037), and the National Natural Science Foundation of China (No.51105174).
文摘Functionalizing and patterning of the silicon surface can be realized simultaneously by the chemomechanical method. The oxide-coated crystalline silicon (100) surface is scratched with a diamond tool in the presence of aryldiazonium salt (C6H5N2BF4). Scratching activates the silicon surface by removing the passivation oxide layer to expose fresh Si atoms. The sur- face morphologies before and after chemomechanical reaction are characterized with atomic force microscopy. Time-of-flight secondary ion mass spectroscopy confirms the presence of C6H5 and provides evidence for the formation of self-assembled monolayer (SAM) on silicon surface via Si-C covalent bonds by scratching the silicon in the presence of C6H5N2BF4. C6H5 groups further bond with surface Si atoms via Si-C covalent bonds as confirmed from infrared spectroscopy results. We propose that chemomechanical reaction, which occurred during scratching the silicon surface, produce C6H5 groups from aryldiazonium salt. The relevant adhesion of SAM is measured. It is found that SAM can reduce the adhesion of silicon. The monolayer can be used as anti-adhesion monolayer for micro/nanoelectromechanical systems components under different environments and operating conditions.
基金Project supported by the National Natural Science Foundation of China (Nos. 40401025 and 49871005)the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0412)
文摘To identify the main sources responsible for soil heavy metal contamination, 70 topsoils were sampled from the Daxing County in the urban-rural transition zone of Beijing. The concentrations of heavy metals Cu, Zn, Pb, Cr, Cd, Ni, As, Se, Hg, and Co; the soil texture; and the organic matter content were determined for each soil sample. Descriptive statistics and geostatistics were used to analyze the data, and Kriging analysis was used to estimate the unobserved points and to map the spatial patterns of soil heavy metals. The results showed that the concentrations of all the soil heavy metals exceeded their background levels with the exception of As and Se. However, only the Cd concentration in some areas exceeded the critical value of the national soil quality standard. The semivariance analysis showed that the spatial correlation distances for soil Cu, Zn, Cr, Cd, As, Ni, and Co ranged from 4.0 to 7.0 km, but soil Se, Pb, and Hg had a larger correlation distance. Soil Co, Se, Cd, Cu and Zn showed a strong spatial correlation, whereas the other soil heavy metals showed medium spatial correlation. Soil heavy metal concentrations were related to soil texture, organic matter content, and the accumulation of heavy metals in the soils, which was because of air deposition and use of water from the Liangshui, Xinfeng, and Fenghe rivers that are contaminated by wastewater and sewage for the purpose of irrigation of fields. Hence, a comprehensive treatment plan for these rivers should be formulated.