Spinosyn A对家蝇生长发育及Na^+,K^+-ATPase、Ca^(2+),Mg^(2+)-ATPase和AChE的影响

研究了Spinosyn A对家蝇(Musca domestica L.)生长发育及其Na+,K+-ATPase、Ca2+,Mg2+-ATPase和AChE的影响。结果表明,SpinosynA对家蝇有杀卵作用,且其化蛹率显著下降;SpinosynA在离体时低浓度抑制家蝇头部Na+,K+-ATPase活性而高浓度酶被激活,活体时在48h内酶活性都受到明显抑制,并随浓度升高而增强;离体时低浓度抑制家蝇Ca2+,Mg2+-ATPase活性而高浓度时酶被激活;活体时24h后该酶被激活。离体时对家蝇头部AChE有弱的抑制作用,但不同处理浓度之间无差异,而在活体时48h内能显著激活AChE活性。

从《长发公主》谈美国动画电影故事的构建

美国第一部动画片诞生于20世纪初,在经历了开创、初步发展、两次繁荣阶段之后,形成了强大并独具风格的动画电影市场。在这百年间的发展中,美国动画电影形成了鲜明的风格,它以剧情片为主,情节曲折生动,主题特色鲜明,这种剧本模式逐渐被世界各国电影制作行业所借鉴。文章从阐述与分析动画电影《长发公主》内容梗概及创作主题着手,对美国动画电影故事的构建展开研究与探讨。

浅析迪士尼动画中的角色造型设计——以《白雪公主和七个小矮人》与《长发公主》为例

《白雪公主和七个小矮人》和《长发公主》都是美国迪士尼动画工作室的代表作品,两部电影的上映时间,前后相差70多年,但都在全球范围内产生了巨大影响。无论是70多年前的手绘动画效果,还是现在纯熟的电脑动画技术,迪士尼动画电影都将角色形象刻画得惟妙惟肖、真切动人。本文从这两部电影中的角色造型设计入手展开比较,探讨影片中角色形象的异同。

美国动画电影《长发公主》的故事构建

美国动画电影有着独特鲜明的影视风格,逐渐被世界各国电影观众所接受和喜爱。《长发公主》就是这样一部动画影片。它主题突出,故事情节曲折,将趣味性与哲理性巧妙融合,体现了美国动画电影的创作思维与价值理念。本文通过分析美国动画电影《长发公主》中的剧情特点与创作结构,阐释美国动画电影故事的建构特点,旨在对动画电影故事的研究有所助益。

目的论视角下对电影《长发公主》字幕翻译的探讨

随着全球一体化的发展,电影已经成为人们了解学习异国文化的重要途径。这里以目的论为指导,就迪斯尼电影《长发公主》的字幕翻译进行分析,以期就电影字幕翻译这一领域的研究进行新视角的探讨。

《长发公主》电影音乐赏析

本文通过对电影《长发公主》的音乐赏析,体会在不同环境下,在剧中出现的主题曲、插曲、场景音乐、背景音乐歌曲等与情节发展密切相关,表现人物的情感,突出情节发展气氛,在艺术上起点描式的作用,并阐释出音乐对影视作品生命力的作用。

长发为君

其实，我知道你喜欢看我长发披肩的样子其实，如果你活着我已经原谅你了其实，我的头发已经留长可是——什么时候，我们结婚呢？

长发与短发

张长发爱留长发，李小毛爱理短发，李小毛理发的次数自然比张长发理发的次数多。 一天，两人相遇了，李小毛对张长发说：“我2个月前与你一起理过发，没想到今天又遇到你。”

AAV-mediated expression of p65shRNA and bone morphogenetic protein 4 synergistically enhances chondrocyte regeneration

BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent years.However,given the complexity of osteoarthritis pathogenesis,single gene manipulation for the treatment of osteoarthritis may not produce satisfactory results.Previous studies have shown that nuclear factorκB could promote the inflammatory pathway in osteoarthritic chondrocytes,and bone morphogenetic protein 4(BMP4)could promote cartilage regeneration.OBJECTIVE:To test whether combined application of AAV-p65shRNA and AAV-BMP4 will yield the synergistic effect on chondrocytes regeneration and osteoarthritis treatment.METHODS:Viral particles containing AAV-p65-shRNA and AAV-BMP4 were prepared.Their efficacy in inhibiting inflammation in chondrocytes and promoting chondrogenesis was assessed in vitro and in vivo by transfecting AAV-p65-shRNA or AAV-BMP4 into cells.The experiments were divided into five groups:PBS group;osteoarthritis group;AAV-BMP4 group;AAV-p65shRNA group;and BMP4-p65shRNA 1:1 group.Samples were collected at 4,12,and 24 weeks postoperatively.Tissue staining,including safranin O and Alcian blue,was applied after collecting articular tissue.Then,the optimal ratio between the two types of transfected viral particles was further investigated to improve the chondrogenic potential of mixed cells in vivo.RESULTS AND CONCLUSION:The combined application of AAV-p65shRNA and AAV-BMP4 together showed a synergistic effect on cartilage regeneration and osteoarthritis treatment.Mixed cells transfected with AAV-p65shRNA and AAV-BMP4 at a 1:1 ratio produced the most extracellular matrix synthesis(P<0.05).In vivo results also revealed that the combination of the two viruses had the highest regenerative potential for osteoarthritic cartilage(P<0.05).In the present study,we also discovered that the combined therapy had the maximum effect when the two viruses were administered in equal proportions.Decreasing either p65shRNA or BMP4 transfected cells resulted in less collagen II synthesis.This implies that inhibiting inflammation by p65shRNA and promoting regeneration by BMP4 are equally important for osteoarthritis treatment.These findings provide a new strategy for the treatment of early osteoarthritis by simultaneously inhibiting cartilage inflammation and promoting cartilage repair.

Growth Control of Quasi-two-dimensional Perovskites:Structure-dependent Exciton and Charge Behavior

While three-dimensional perovskites have high defect tolerance and an adjustable bandgap,their charges tend to be free rather than forming excitons,making them unsuitable for use in efficient light-emitting diodes(LEDs).Rather,quasi-two-dimensional(Q-2D)perovskites offer high photoluminescence quantum yield along with the advantages of bulk perovskites,making them ideal for high-performance LEDs.In Q-2D perovskites,the structure(which includes factors like crystal orientation,phase distribution,and layer thickness)directly influences how excitons and charge carriers behave within the material.Growth control techniques,such as varying the synthesis conditions or employing methods,allow for fine-tuning the structural characteristics of these materials,which in turn affect exciton dynamics and charge transport.This review starts with a description of the basic properties of Q-2D perovskites,examines crystal growth in solution,explains how structure affects energy transfer behavior,and concludes with future directions for Q-2D perovskite LEDs.By understanding and optimizing the structure-dependent behavior,researchers can better control exciton dynamics and charge transport,which are crucial for enhancing the performance of optoelectronic devices like solar cells and LEDs.

Association of Polymorphisms of Leptin Gene with Body Weight and Body Sizes Indexes in Chinese Indigenous Cattle

PCR-SSCP was used to analyze the polymorphism of leptin gene in 539 samples of six cattle breeds, namely Nanyang （NY）, Qinchuan （QC）, Jiaxianred （JXR）, Xizhen （XZ）, Luxi （LX）, and Holstein cow （HOL） breeds. PCR products with a 330 bp were amplified and sequenced. The results showed that the frequencies of alleles A/B of NY, QC, JXR, XZ, LX, and HOL breeds were 0.558/0.442, 0.492/0.508, 0.571/0.429, 0.658/0.342, 0.591/0.409, and 0.615/0.385, respectively. The association of variations of leptin gene with growth traits in NY, QC, JXR breeds was analyzed. Some indexes of the individuals with genotype BB were higher than that with genotype AA and AB in NY breed, such as the indexes of body length, heart length, body weight, hucklebone width, body height, and average day gain. The height at hip cross of the individuals with genotype BB was higher than that of those with genotype AA and AB in QC breed （P 〈 0.05）. So leptin gene may be one of the candidate genes for growth traits with height at hip cross, but not for body weight, heart length, and body length trait. However, the height at hip cross and hucklebone width of the individuals with genotype AB and BB were higher than that of those with genotype AA in JXR breed （P 〈 0.05）, but the difference was not statistically significant in body weight and body sizes （body height, body length, and heart length）. And the polymorphisms in leptin gene were caused by G→T transversion at the 66th bp position, A→C transversion at the 67th bp position and G→T transversion at the 299th bp position. These results may be applied to marker-assisted selection of Chinese cattle breeds.

Camptothecin and 10-Hydroxycamptothecin Accumulate Differentially Under Specific Developmental Control in Camptotheca acuminata

Camptotheca acuminata Decaisne is a tree unique to China, producing terpenoid indole alkaloid (TIA), camptothecin (CPT), and 10-hydroxycamptothecin (HCPT), which possesses strong anti-cancer bioactivity. The dynamics of HCPT and CPT during seed maturation, seed germination, postgerminative development and daily growth were measured by High Performance Liquid Chromatography (HPLC). This article provides some evidence for presenting the theory that each characteristic of HCPT and CPT accumulation is under developmental regulation and then attempt to elucidate the metabolic relationships between them. HCPT accumulates particularly in young tissues and organs and is relatively unstable, while the distribution of CPT is more ubiquitous and stable. Their different distribution properties suggest that HCPT and CPT are differentially regulated and play distinct roles:during development.

Effects of Plastic Film Mulching on Physical Characters of Soil and Yield and Yield Components of Sweet Potato

Field experiments were carried out to study the effects of plastic film mulching on soil physical characters, including soil temperature, soil moisture content and soil bulk density, and yield and yield components of sweet potato. The results showed that plastic filming mulching increased soil temperature. Considering the soil temperature-increasing effect, the treatments ranked as black plastic film treatment 〉 white plastic film treatment 〉 control. However, with the deepening of soil layer, the warming effect of plastic film mulching was weakened. Black or white plastic film mulching was conducive to low T/R value, especially in the early growth stage of sweet potato. Plastic film mulching significantly improved the storage root yield of sweet potato. In terms of yield-improving effect, the treatments ranked as black plastic film treatment 〉 white plastic film treatment 〉 control. The storage root num- ber per plant showed a downward trend, but the weight of single storage root was increased.

Effects of Tiller-inhibitor on Growth and Yield Formation of Super Early Hybrid Rice Jinyou458

[Objective]The paper was to study the effect of tiller-inhibitor on the growth and yield formation of super early rice Jinyou 458.[Method] Two treatments including spraying tiller-inhibitor（TI） and spraying water（CK） at the SN-n stage were set in the test,the effect of tiller-inhibitor on yield and its components,leaf and plant morphology,field microclimate at booting stage and quality characteristics of rice population were studied.[Result] Spraying tiller-inhibitor could effectively reduce the occurrence of invalid and inefficient tillers,increase the proportion of high effective tillers（tiller with 4 or more leaves） in tiller composition at the maximal tiller stage.The panicle length,spikelets per panicle,spikelets density,number of secondary branches and the secondary spikelets,seed setting rate of rice plant sprayed with tiller-inhibitor were significantly higher than CK.Meanwhile,spraying tiller-inhibitor could increase plant height,biomass weight and leaf area index of rice population during middle and late stages of rice growing,improve the leaf temperature of top three leaves and the light transmittance of rice population at the booting stage,increase the leaf SPAD value,thus enhance photosynthetic capacity of rice plants.It also showed the characteristics of elongating the internode of rice plant and decreasing the stem thickness,etc.[Conclusion] The panicle-bearing rate of stems and tillers,effective panicles,spikelets per panicle,seed setting rate and 1 000-grain weight had coordinately increased after spraying tiller-inhibitor,thus increased the yield.

Artificial incubation and growth observation for the nestlings of Great Bustard (Otis tarda)

Growth indicators including weight, body length, wings length, tail length, tarsus, gape, the third toe and head width of 21 nestlings of Great Bustard (Otis tarda) were measured and investigated in Harbin Zoo, Harbin, China during 1999-2002, and methods on successfully fostering nestlings of the bird were also summarized in this article. The results showed: the Great Bustard is a kind of premature bird and its birth weight was 86.31?.56g (N=21); environmental temperature for the neonatal nestlings should be controlled at 36C; the feeding principle having many meals but little food at each for the nestlings should be followed; since six weeks after birth, nestlings of both gender began to show significant difference in body weight, the weight of male was 1.8 times of that of the female after fourteenth week, and by weight and body figure sexual identity could be easily discerned when 3 or 4 months old; There is no significant difference in growth and development of all organs between male and female nestlings and organ growth curves were fit into Logistic equation.

Effects of Different Intercropping Modes on Growth, Yields and Economic Benefit of Cassava and Peanut in Symbiotic Period

[Objective] This study was conducted to investigate effects of different intercropping modes on growth, yields and economic benefit of cassava and peanut in symbiotic period. [Method] With sole cropping of cassava （M1） and sole cropping of peanut （M2） as control groups, effects of intercropping of cassava with 1 row, 2 rows and 3 rows of peanut （M3, M4 and M5）on crop growth, yields and economic benefit were studied. [Result] Intercropping affected both growth and yields of cassava and peanut. Growth competition existed between cassava and peanut, and plant heights of cassava and peanut changed similarly. In late stages of intercropping, treatments M1, M2 and M5 showed higher plant heights under no nitrogen application, while treatment M3 and M4 exhibited higher plant heights under nitrogen application; intercropping improved leaf temperature, but no obvious law could be observed among different intercropping treatments; and intercropping improved total dry matter amount, which was the highest in M5 in root expanding stage and on the 30th day of the expanding stage, and the highest in M4 on the 60th day of the expanding stage. Intercropping reduced the yield of single plant, but improved the economic benefit of red upland soil; and under no nitrogen application and nitrogen application, cassava yields decreased by 25.35% and 14.55%, respectively, peanut yields decreased by 28.76% and 52.60%, respectively, while economic benefit increased by 72.90% and 56.82%, respectively. [Conclusion] Compared with sole cropping, interplanting cassava with 1 row, 2 rows or 3 rows of peanut could all improve economic benefit, and the economic benefit increased with number of rows of interplanted peanut increasing.

Effects of Different Light Qualities on Activity and Gene Expression of Caspase-like Proteases in Tobacco Leaves

[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the changes of chlorophyll content, activity and gene expression of caspase-like proteases in the tobacco leaves were studied. [Results] Compared with treatments of white, red and yellow film, blue and purple films delayed the decrease of chlorophyll content and senescence of tobacco leaves at the late growth stage, and relatively lowered the activity and gene expression of caspase-like proteases during growth, development and senescence periods. [Conclusion] Different light qualities exhibited various effects on the growth, development and senescence of tobacco leaves, possibly by affecting the activity and gene expression of caspase-like proteases to some extent.

Effect of Fulvic Acid on Growth and Yield Components of Direct Seeding Rice

ObjectiveThis study aimed to investigate the effect of fulvic acid on the growth and yield components of direct seeding rice （Nanjing 44）. MethodThe rice seeds were soaked in 0 （water as a control）, 1, 2, 4 and 6 g/L fulvic acid （FA） before sowed. Then, the rice morphological indices, leaf chlorophyll content, photosynthesis parameters, root activity and chlorophyll fluorescence parameters were measured in the following field studies. ResultCompared with the control, the leaf area index, chlorophyll content, net photosynthetic rate, stomatal conductance and dry matter weight and some fluorescence parameters such as the maximum photochemical efficiency of photosystem II （PSII） （Fv/Fm）, excitation energy capture efficiency of opened PSII reaction center （Fv’/Fm’） and efficiency of the open reaction centre （ΦPS II） increased by different levels at both jointing stage and heading stage of direct seeding rice, whose seeds were soaked by FA with different concentrations. High FA concentration （4-6 g/L） significantly increased the cultivated rice leaf area index, chlorophyll content, net photosynthetic rate, stomatal conductance, transpiration rate and dry matter weight by 10.32% -22.88% , 5.88% -13.11% , 12.16% -26.84% , 11.43% -88.46% , 10.63% -21.63% , 18.49% -19.68% , respectively, thereby improving the physiological function and light energy transform efficiency of rice at the growth stage. With FA concentration increasing, the yield, effective panicles, grain number per panicle and seed setting rate were increased significantly compared with the control by 17.52%-18.71%, 3.46%-3.85%, 6.30%- 6.51% and 7.82%-8.69% respectively. ConclusionSoaking rice seed with FA could be considered as an effective way to improve the rice competitiveness at early growth stage.

Comparison of Growth and Development Traits among Crossbreds of Dorset and Local Sheep Varieties in Gansu Province

In order to improve the meat production performance of local sheep varieties in Gansu Province, Dorset was introduced to crossbreed with the local sheep varieties, including Tan sheep, Small Tail Han sheep and Mongolia sheep. The offspring of different crossbreeding combinations were sampled randomly at different growth stages, and their growth and development traits were measured so as to screen out the best crossbreeding mode. The results showed that under the same crossbreeding mode, the growth rate of F3 was higher than that of F2, and of F2 was higher than that of F1. Among the F3 population, the growth rates of Dorset ×Han and Dorset × Mongolia hybrids were higher. Compared with those of Dorset ×Tan F3 hybrids, the body weights of male and female Dorset × Han and Dorset ×Mongolia F3 hybrids were increased by 5.59%, 4.40%, 5.93% and 3.76%, respectively. Among the F2 population, the growth rates of Dorset × Han and Dorset ×Mongolia hybrids were also higher. The body weights of male and female Dorset ×Han and Dorset × Mongolia F2 hybrids were higher than those of Dorset × Tan ×Han F2 hybrids by 5.99%, 3.67%, 9.80% and 5.00%, respectively. In the F1 population, the growth rates of Dorset × Han and Dorset × Mongolia hybrids were higher.Compared with those of Tan × Han F1 hybrids, the body weights of male and female Dorset × Han and Dorset × Mongolia F1 hybrids were increased by 11.32%,5.22%, 7.60% and 7.20%, respectively. Therefore, in the feeding area of Small Tai Han sheep, Mongolia sheep and Tan sheep, Dorset was the best sire for producing hybrid lambs. The economic benefit of crossbred offspring was obvious.

Dynamic Characteristics of Growth and Development of Xiangsha Taro in Jingjiang

The growth and development of Jingjiang Taro [Colocasia esculenta（L.） Schott] were observed, laying foundation for highly-yielding cultivation technology system of Xiangsha taro. The results indicate that germination of the second taro happened from 46 d after sprouting （7-8 leaves）; the first taro began to expand, from 125 d after sprouting （12 leaves） when the the second taro was been formed; the third taro began to form from 141 to 150 d after sprouting （15-16 leaves）. The fresh weight of the second and third taros quickly increased from the time of 15 leaves to harvest. Early September is a transformation term of Xiangsha taro from vegetative body dominant to corm growth dominant.