AIM: To prepare a kind of magnetic iron-dextran nanopartides that was coated with anti-E.coli O157:H7 IgG, analyze its application conditions, and try to use it to isolate E.coli O157:H7 from foods. METHODS: Magnetic ...AIM: To prepare a kind of magnetic iron-dextran nanopartides that was coated with anti-E.coli O157:H7 IgG, analyze its application conditions, and try to use it to isolate E.coli O157:H7 from foods. METHODS: Magnetic iron-dextran nanopartides were prepared by the reaction of a mixture of ferric and ferrous ions with dextran polymers under alkaline conditions. The particles were coated with antiserum against E.coli O157: H7 by the periodate oxidation-borohydride reduction procedure. The oxidation time, amount of antibody coating the particles, amount of nanoparticles, incubation time and isolation time were varied to determine their effects on recovery of the organisms. Finally, the optimum conditions for isolating E.coli O157:H7 from food samples were established. RESULTS: E.coli O157:H7 can be isolated from samples within 15 min with the sensitivity of 101 CFU/mL or even less. In the presence of 108 CFU/mL of other organisms, the sensitivity is 101-102 CFU/mL. Nonspecific binding of other bacteria to the particles was not observed. Two and a half hours of enrichment is enough for the particles to detect the target from the food samples inoculated with 1 CFU/g. CONCLUSION: Isolation of target bacteria by immuno magnetic nanoparticles is an efficient method with high sensitivity and specificity. The technique is so simple that it can be operated in lab and field even by untrained personnel.展开更多
For the first time an anti-shock packaging model of an acoustic-vibration sensor system has been designed by using the shocking isolation principle. The finite element analysis has been applied for design and simulati...For the first time an anti-shock packaging model of an acoustic-vibration sensor system has been designed by using the shocking isolation principle. The finite element analysis has been applied for design and simulation of the model. The effects of Young’s modulus of anti-shock rubber on naturally occurring frequencies of the combination of rubber and an acoustic sensor chip were analyzed. The displacement of the acoustic sensor chip is loaded with force. The results of static analysis and harmonic analysis show that while increasing Young’s modulus of anti-chock rubber, the first five natural frequencies of the package body also increases. Yet the displacement of the acoustic sensor chip around the resonant frequency decreases. The results of static and transient analysis show that the displacement of the acoustic sensor chip decreases with the increase of Young’s modulus of anti-chock rubber being loaded with either transient force or static force at the bottom of the combination of rubber and acoustic sensor chip.展开更多
The Epic hero Beowulf is honored as a great leader of his nation, but such a hero is isolated. He was not born a hero. In his growth, he is often detached from his family and society. Isolation makes up the critical p...The Epic hero Beowulf is honored as a great leader of his nation, but such a hero is isolated. He was not born a hero. In his growth, he is often detached from his family and society. Isolation makes up the critical part of the process of being a great hero.展开更多
The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5' and 3...The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5' and 3' ends was 1,143 base pairs (bp) and 1,137 bp encoding 380 and 378 amino acids in the K-11 strain and HS strain, respectively. In addition, higher substitutions both in base and amino acid residues occurred more frequently between the former control (Hd-rR strain) and HS strains than the K-I 1 strain. Approximately similar values in polymorphism, as assessed by restriction endonuclease digestion, were detected when utilizing 20 different enzymes. Therefore, the evolutionary processes were unlikely to involve common ancestors, especially between the K-11 strain and HS strains, in O. latipes populations in Japan. The sequence had been deposited in GenBank Data Base under accession number AB480878 (K-I I strain) and AB480879 (HS strain).展开更多
The heat How generated from the infinite rock mass surrounding the underground tunnels is a major cause for the increasing cooling demands in deep mine tunnels.Insulation layers with lower thermal conductivities on tu...The heat How generated from the infinite rock mass surrounding the underground tunnels is a major cause for the increasing cooling demands in deep mine tunnels.Insulation layers with lower thermal conductivities on tunnel walls and roof ceilings are believed to supply a thermo-barrier for heat abatement.However,it is found that no systematic theoretical investigations were made to predict and confirm the effectiveness of underground thermal insulation.Specifically,investigations on the underground insulation problems involving heat flows through the semi-infinite hot rock mass and insulation layer were not sufficient.Thus,in this paper,the thermal characteristics,accompanied with heat flow through the semi-infinite rock mass and the insulation layer,were modeled by both analytical and numerical methods with focus on underground mine tunnels.The close agreements between models have indicated that the thermal insulation applied on tunnel surfaces is able to provide promising heat abatement effects.展开更多
Analogizing with the heat conduction process, the entransy dissipation extremum principle for thermal insulation process can be described as: for a fixed boundary heat flux (heat loss) with certain constraints, the th...Analogizing with the heat conduction process, the entransy dissipation extremum principle for thermal insulation process can be described as: for a fixed boundary heat flux (heat loss) with certain constraints, the thermal insulation process is optimized when the entransy dissipation is maximized (maximum average temperature difference), while for a fixed boundary temperature, the thermal insulation process is optimized when the entransy dissipation is minimized (minimum average heat loss rate). Based on the constructal theory, the constructal optimizations of a single plane and cylindrical insulation layers as well as multi-layer insulation layers of the steel rolling reheating furnace walls are carried out for the fixed boundary temperatures and by taking the minimization of entransy dissipation rate as optimization objective. The optimal constructs of these three kinds of insulation structures with distributed thicknesses are obtained. The results show that compared with the insulation layers with uniform thicknesses and the optimal constructs of the insulation layers obtained by minimum heat loss rate, the optimal constructs of the insulation layers obtained by minimum entransy dissipation rate are obviously different from those of the former two insulation layers; the optimal constructs of the insulation layers obtained by minimum entransy dissipation rate can effectively reduce the average heat loss rates of the insulation layers, and can help to improve their global thermal insulation performances. The entransy dissipation extremum principle is applied to the constructal optimizations of insulation systems, which will help to extend the application range of the entransy dissipation extremum principle.展开更多
基金Supported by the National High-technology Research and Development Program of China (863 Program), No. 2003AA302260
文摘AIM: To prepare a kind of magnetic iron-dextran nanopartides that was coated with anti-E.coli O157:H7 IgG, analyze its application conditions, and try to use it to isolate E.coli O157:H7 from foods. METHODS: Magnetic iron-dextran nanopartides were prepared by the reaction of a mixture of ferric and ferrous ions with dextran polymers under alkaline conditions. The particles were coated with antiserum against E.coli O157: H7 by the periodate oxidation-borohydride reduction procedure. The oxidation time, amount of antibody coating the particles, amount of nanoparticles, incubation time and isolation time were varied to determine their effects on recovery of the organisms. Finally, the optimum conditions for isolating E.coli O157:H7 from food samples were established. RESULTS: E.coli O157:H7 can be isolated from samples within 15 min with the sensitivity of 101 CFU/mL or even less. In the presence of 108 CFU/mL of other organisms, the sensitivity is 101-102 CFU/mL. Nonspecific binding of other bacteria to the particles was not observed. Two and a half hours of enrichment is enough for the particles to detect the target from the food samples inoculated with 1 CFU/g. CONCLUSION: Isolation of target bacteria by immuno magnetic nanoparticles is an efficient method with high sensitivity and specificity. The technique is so simple that it can be operated in lab and field even by untrained personnel.
基金Sponsored by the Creativity Ability Fund Project for Cadreman of General Provincial University of Heilongjiang(Grant No.1053G033).
文摘For the first time an anti-shock packaging model of an acoustic-vibration sensor system has been designed by using the shocking isolation principle. The finite element analysis has been applied for design and simulation of the model. The effects of Young’s modulus of anti-shock rubber on naturally occurring frequencies of the combination of rubber and an acoustic sensor chip were analyzed. The displacement of the acoustic sensor chip is loaded with force. The results of static analysis and harmonic analysis show that while increasing Young’s modulus of anti-chock rubber, the first five natural frequencies of the package body also increases. Yet the displacement of the acoustic sensor chip around the resonant frequency decreases. The results of static and transient analysis show that the displacement of the acoustic sensor chip decreases with the increase of Young’s modulus of anti-chock rubber being loaded with either transient force or static force at the bottom of the combination of rubber and acoustic sensor chip.
文摘The Epic hero Beowulf is honored as a great leader of his nation, but such a hero is isolated. He was not born a hero. In his growth, he is often detached from his family and society. Isolation makes up the critical part of the process of being a great hero.
文摘The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5' and 3' ends was 1,143 base pairs (bp) and 1,137 bp encoding 380 and 378 amino acids in the K-11 strain and HS strain, respectively. In addition, higher substitutions both in base and amino acid residues occurred more frequently between the former control (Hd-rR strain) and HS strains than the K-I 1 strain. Approximately similar values in polymorphism, as assessed by restriction endonuclease digestion, were detected when utilizing 20 different enzymes. Therefore, the evolutionary processes were unlikely to involve common ancestors, especially between the K-11 strain and HS strains, in O. latipes populations in Japan. The sequence had been deposited in GenBank Data Base under accession number AB480878 (K-I I strain) and AB480879 (HS strain).
基金The financial support from an Engage Grant in the Natural Sciences and Engineering Research Council(NSERC),Canada,is gratefully acknowledged
文摘The heat How generated from the infinite rock mass surrounding the underground tunnels is a major cause for the increasing cooling demands in deep mine tunnels.Insulation layers with lower thermal conductivities on tunnel walls and roof ceilings are believed to supply a thermo-barrier for heat abatement.However,it is found that no systematic theoretical investigations were made to predict and confirm the effectiveness of underground thermal insulation.Specifically,investigations on the underground insulation problems involving heat flows through the semi-infinite hot rock mass and insulation layer were not sufficient.Thus,in this paper,the thermal characteristics,accompanied with heat flow through the semi-infinite rock mass and the insulation layer,were modeled by both analytical and numerical methods with focus on underground mine tunnels.The close agreements between models have indicated that the thermal insulation applied on tunnel surfaces is able to provide promising heat abatement effects.
基金supported by the National Key Basic Research and Development Program of China (‘973’ Program) (Grant No. 2012CB720405)the National Natural Science Foundation of China (Grant No. 51176203)the Natural Science Foundation for Youngsters of Naval University of Engineering (Grant No. HGDQNJJ11008)
文摘Analogizing with the heat conduction process, the entransy dissipation extremum principle for thermal insulation process can be described as: for a fixed boundary heat flux (heat loss) with certain constraints, the thermal insulation process is optimized when the entransy dissipation is maximized (maximum average temperature difference), while for a fixed boundary temperature, the thermal insulation process is optimized when the entransy dissipation is minimized (minimum average heat loss rate). Based on the constructal theory, the constructal optimizations of a single plane and cylindrical insulation layers as well as multi-layer insulation layers of the steel rolling reheating furnace walls are carried out for the fixed boundary temperatures and by taking the minimization of entransy dissipation rate as optimization objective. The optimal constructs of these three kinds of insulation structures with distributed thicknesses are obtained. The results show that compared with the insulation layers with uniform thicknesses and the optimal constructs of the insulation layers obtained by minimum heat loss rate, the optimal constructs of the insulation layers obtained by minimum entransy dissipation rate are obviously different from those of the former two insulation layers; the optimal constructs of the insulation layers obtained by minimum entransy dissipation rate can effectively reduce the average heat loss rates of the insulation layers, and can help to improve their global thermal insulation performances. The entransy dissipation extremum principle is applied to the constructal optimizations of insulation systems, which will help to extend the application range of the entransy dissipation extremum principle.