从《高达》试论日本动画成功之道

动画作为一种出色的艺术表现形式，早已融入了我们生活的方方面面。诞生于1979年，作为占据全球动画领域半壁江山的日本动画代表之作，同时也成为其象征符号的《机动战士——高达》系列，历经32载依然热度不减，带来巨大的文化影响和经济收益。是什么创造了《高达》的神话？本文将从《高达》的剧本内涵、角色设定、声优配乐及周边市场四个方面进行分析，并结合实际为国产动画的发展寻求一点启示。

冬天里的一把火 高达周边

随着动画《高达Seed Destiny》的热播，相关周边也开始不断推出，本次我们就推出以《高达Seed Destiny》为主动的高达周边主题的购物。

宇宙中起舞 高达OL

《高达Online：一年战争篇》（以下简称《高达OL》）是以著名动画高达系列之《一年战争篇》为故事背景开发的一款在线战略模拟游戏，它再现了高达原作中地球圈的真实环境，并拥有能同时容纳16万玩家在线的系统，在防止服务器断线方面有很大改进，历经两件等待，它终于登陆中国台湾，即将开始测试，下面就让我们来一睹芳容。

High resolution GPR and its experimental study

We develop a high resolution ground penetrating radar system （LANRCS-GPR） based on the E5071B Vector Network Analyzer （VNA）. This system takes advantage of a wideband and adjustable frequency domain ground penetrating radar system and adds the characteristics of a network analyzer with ultra-wideband and high precision measurement. It adopts the LAN mode to concatenate system control that reduces construction cost and makes the system easy to expand. The high resolution ground penetrating radar system carries out real time imaging using F-K migration with high calculation efficiency. The experiment results of the system indicate that the LANRCS-GPR system provides high resolution and precision, high signal-to-noise ratio, and great dynamic range. Furthermore, the LANRCS-GPR system is flexible and reliable to operate with easy to expand system functions. The research and development of the LANRCS-GPR provide the theoretical and experimental foundation for future frequency domain ground penetrating radar production and also can serve as an experimental platform with high data gathering precision, enormous information capability, wide application, and convenient operation for electromagnetic wave research and electromagnetic exploration.

Cloning, E. coli Expression and Molecular Analysis of a Novel Sesquiterpene Synthase Gene from Artemisia annua

A 1 886 bp full-length sesquiterpene synthase (AaSES) cDNA was cloned front a high-yield Artemisia annua L. strain 001 by a rapid amplification of cDNA end (RACE) strategy. AaSES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua , 48% identical to amorpha-4, 11-diene synthase from A. annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38 % identical to vetispiradiene synthase front H. muticus, 41 % identical to the, delta-cadinene synthase from cotton. The coding region of the cDNA was inserted into a procaryotic expression vector pET-30a and overexpressed in E. coli BL21 ( DE3). The cyclase proteins extracted front bacterial culture were found largely in an insoluble protein fraction. AaSES expresses in leaves, stems a-rid flowers, not in roots as indicated by Northern blotting analysis.

Effect of the Expression of HSP 70 Gene on the Fertility of Sorghum 1

It was found that the Cytoplasmic Male Sterility (CMS) line of sorghum 3197A (3A) can be induced to become fertile by heat shock treatment, whereas the maintainer line 3197B (3B) is fertile, whether it is heat shocked or not. During the pollen mother cell (PMC) stage, with heat shock treatment, the 3A line changed into fertile from sterile, and five protein bands: 70kD (HSP 70 ), 31kD, 24kD, 18kD and 16kD were found. And identical bands were also appeared in 3B line with similar treatment. By comparing the amount of protein in mitochondria during ear stage of 3A sorghum, the amount of protein increased by 2.7 times, and was almost as much as that in 3B. A comparison of the heat shock proteins (HSPs) of mitochondria in 3A and 3B lines revealed that HSPs were encoded by the nuclear DNA and transported to the mitochondria. Moreover, HSPs were more heat stable in 3B than in 3A line, and this may be related to the stability of fertility in 3B line. To demonstrate the correlation between fertility and HSPs, DNA complementary to HSP 70 was injected into ears of 3A and 3B line. It is found that under normal conditions, there was no HSP 70 mRNA in 3A line, whereas after heat shock this mRNA appeared, and the antisense cDNA could combine with the target RNA of 3B either dealt with normal temperature or heat shocked. These results are discussed within the frame of fertility versus sterility in sorghum.

Hyperspectral image classification based on spatial and spectral features and sparse representation

To minimize the low classification accuracy and low utilization of spatial information in traditional hyperspectral image classification methods, we propose a new hyperspectral image classification method, which is based on the Gabor spatial texture features and nonparametric weighted spectral features, and the sparse representation classification method（Gabor–NWSF and SRC）, abbreviated GNWSF–SRC. The proposed（GNWSF–SRC） method first combines the Gabor spatial features and nonparametric weighted spectral features to describe the hyperspectral image, and then applies the sparse representation method. Finally, the classification is obtained by analyzing the reconstruction error. We use the proposed method to process two typical hyperspectral data sets with different percentages of training samples. Theoretical analysis and simulation demonstrate that the proposed method improves the classification accuracy and Kappa coefficient compared with traditional classification methods and achieves better classification performance.

AUTOFOCUSING OF SYNTHETIC HRR RADAR USING STEPPED FREQUENCY WAVEFORMS

Most operating radar systems don′t have sufficient frequency bandwidth to produce high range resolution(HRR) profile of a target. But we can use stepped frequency waveform in a narrow band coherent radar to obtain the HRR profile of a target. For moving targets which are of great importance in practical radar usage, autofocusing,i.e. phase correction, is a necessary and critical step of the synthetic HRR processing. The purpose of autofocusing is to remove the radial motion effect of the target from radar echoes, and only reserve the stepped frequency effect which is the basis of synthetic HRR capability. We investigate two autofocusing approaches for synthetic HRR radars using stepped frequency waveform in this paper. The first is motion fitting method. This method depends on a certain parametric model, and is computationally expensive. Then we propose the iterative dominant scatterer method. It is robust, non parametric and simple in computation in comparison with the motion fitting method. Experimental results based on data acquired by using a metallised scale model B 52 in a microwave anechoic chamber reveal the validity and effectiveness of the method.

A Modeling-Based Radar Superresolution Approach

On the basis of modeling of multiple targets and radar returning signals, a new method for radar range superresolution is introduced in this paper. This method tums the resolution of multiple closely spaced targets into an identification of the target impulse response functions. The least-square (LS) method is then used to estimate impulse resoonse functions of the targets. The concept of radar range resolution probability is also introduced.It is shown from the simulation results that the new superresolution method is much efficient, and has advantages such as having a simple structure, as well as high resolution.

Cloning of H6H Gene from Atropa belladonna and Construction of the Efficient Plant Expression Vector

[Objective] The aim was to clone H6H gene from Atropa belladonna and construct an efficient plant expression vector.[Method] The coding sequence of H6H（Hyoscyamine 6β-hydroxylase）was cloned from Atropa belladonna with RT-PCR.Then,the sequence was subcloned into the reconstructed plant binary expression vector p2301 to construct the recombinant vector p2301-H6H,which was then introduced into Agrobacterium tumefaciens strain LBA4404 and Agrobacterium rhizogenes strain C58C1,respectively.[Result] The engineering bacteria p2301-H6H-LBA4404 and p2301-H6H-C58C1 which could be directly used in genetic improvement were obtained.[Conclusion] The present research provided basis for the increasing of alkaloid content of Atropa belladonna by plant genetic engineering technology.

OPTIMIZATION OF WEIGHTED HIGH-RESOLUTION RANGE PROFILE FOR RADAR TARGET RECOGNITION

For the recognition of high-resolution range profile （HRRP） in radar, the weighted HRRP can reduce the instability of range cells caused by the attitude change of targets. A novel approach is proposed to optimize the weighted HRRP. In the approach, the separability of weighted HRRPs in different targets is measured by de- signing an objective function, and the weighted coefficients are computed by using the gradient descent method, thus enhancing the influence of stable range cells. Simulation results based on five aircraft models show that the approach can effectively optimize the weighted HRRP and improve the recognition accuracy.

High Frequency of GFP Gene Transient Expression in Electroporated Zygotes and Early Proembryos of Wheat

Green fluorescent protein (GFP) gene was successfully transferred into the isolated zygotes and early proembryos of wheat (Triticum aestivum L.) by electroporation. A frequency, as high as 46.7% of GFP gene transient expression in early proembryos, was achieved under 150 V/cm electric field strength, 25 muF capacitor, 200 mug/mL of linear plasmid DNA and an electroporation buffer at pH 7.2. Compared with five-day-old proembryos, the zygotes and early proembryos needed lower optimum strength of electric field. After culturing in KM8p medium, the electroporated early proembryos divided and GFP gene expression was observed in daughter cells and subsequent divisions. There was no mosaicism of gene expression in the zygotes and 2-, 4- and 8-celled proembryos.

Computations on the Earth Terrain Return of Pulse Doppler Radar Altimeter

Concerning the PDRA (pulse doppler radar altimeter) designing and evaluation, owing to that the specifications of PDRA should be adaptively fixed according to the ETR (earth terrain return), and that in certain stages of product evaluation of PDRA which means the designations of PDRA are successful or not, the usage of ETR are indispensable, so the terrain return from spherical earth is critically important. A complete analytic derivation of the antenna shot section model of PDRA and the bright section model constrained by pulse emitted from antenna are given. Furthermore, the doppler effect mode and the earth terrain RCF (radar crossing factor) model are constructively analyzed. Then, the computing methodology on PDRA, which are used to compute the scattering power, scattering doppler spectrum, and the scattering signal, is studied. Besides, in order to check the correctness and efficiency of the algorithm, computing examples of ETR (earth terrain return) under the supposing premises are furnished. Finally, the conclusion is drawn that the models and algorithm are rational, the computational precise is satisfactory, the cost of computing time is low.

Predicting Arabidopsis thaliana Gene Function by Transitiving Co-expression in Shortest-path

The present paper predicted the function of unknow genes by analyzing the co-expression data of Arabidopsis thaliana from biological pathway based on the shortest-path algorithm. This paper proposed that transitive co-expression among genes can be used as an important attribute to link genes of the same biological pathway. The genes from the same biological pathway with similar functions are strongly correlated in expression. Moreover,the function of unknown genes can be predicted by the known genes where they are strongly correlated in expression lying on the same shortest-path from the biological pathway. Analyzing the Arabidopsis thaliana from the biological pathway,this study showed that this method can reliably reveal function of the unknown Arabidopsis thaliana genes and the approach of predicting gene function by transitiving co-expression in shortest-path is feasible and effective.

High expression of △Np73 predicts poor prognosis in NSCLC cancer patients

Objective: To study the expression of △Np73, an isoform of the p53 homologue p73, in the different stages of human non-small-cell lung cancer (NSCLC) and the association of△Np73 expression with in patient survival. Methods: Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to study the expression of△Np73 mRNA in 51 resected NSCLC tissues. Its relation to clinicopathological factors and survival outcome were analyzed. Results: The positive rate and expression level of△Np73 mRNA in the cancer tissues were significantly higher than that in the matched non-cancer lung tissues. The incidence of positive expression of△Np73 was 50. 0% , 52. 6% , and 87. 5% in patients with stageⅠ,Ⅱ, andⅢ, respectively. Positive expression of△Np73 was associated with pathological TNM stage (P = 0. 046), while not with age, gender, histological type and differentiation status. Survival rate of patients with high△Np73 mRNA was significantly poorer than those with low△Np73 mRNA levels (P<0. 001). Multivariate analysis revealed that△Np73 mRNA levels were a significant prognostic factor, independent of the other conventional prognostic factors. Conclusion: NSCLC has over-expression of△Np73 mRNA, which is closely related to TNM stages and prognosis of patients with NSCLC. These results suggest that measurement of△Np73 mRNA levels in tumor tissues might be useful as a promising predictor for the prognosis of patients with NSCLC.

THE INCREASE IN PLASMINOGEN ACTIVATOR INHIBITOR TYPE-1 EXPRESSION BY STIMULATION OF ACTIVATORS FOR PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTHELIAL CELLS

Objective.To investigate the effect of peroxis ome proliferator-activated recept ors(PPARs )activators on plasminogen activator inhibitor ty pe-1(PAI-1)expression in human umbilical vein e ndothelial cells and the possi-ble mechanism.Methods.Human umbilical vein endothelial ce lls(HUVECs )were obtained from normal fetus,and cul-tured conventionally.Then the HUVECs were exposed to test agents(linolenic acid,linoleic acid,oleic acid,stearic acid and prostaglandin J 2 respectively)in varying concentrations with fresh media.RT -PCR and ELISA were applied to determine the expression of PPARs and PAI-1in HUVECs.Results.PPARα,PPARδand PPARγmRNA were detected by using RT-PCR in HUVECs.Treatment of HUVECs with PPARαand PPARγactivators---linolenic acid,linoleic acid,oleic acid and prostaglandin J 2 respectively,but not with stearic a cid could augment PAI-I mRNA expression and protein secretion in a concentration-dependent manner.However,the mRNA expressions of 3subclasses of PPAR with their activators in HUVECs were not changed compared w ith controls.Conclusion.HUVECs express PPARs.PPARs activators may increase PAI-1expression in ECs,but the underlying mechanism remains uncle ar.Although PPARs expression was not enhanced after stimulated by their activators in ECs,the role of functionally active PPARs in regulating PA I-1expression in ECs needs to be further investigated by using transient gen e transfection assay.

Sea State Bias Estimation with Least Absolute Shrinkage and Selection Operator(LASSO)

With the sea-level rising,the measurement of sea surface height(SSH) is attracting more and more attention in the area of oceanography.Satellite radar altimeter is usually used to measure the SSH.However,deviation between the measured value and the actual one always exists.Among others,the sea state bias(SSB) is a main reason to cause the deviation.In general,one needs to estimate SSB first to correct the measured SSH.Currently,existing SSB estimation methods more or less have shortcomings,such as with many parameters,high prediction error and long training time.In this paper,we introduce an effective and efficient linear model called LASSO to the SSB estimation.The LASSO algorithm minimizes the residual sum of squares under the condition that the sum of the absolute values of each coefficient is less than a certain constant.In the implementation of LASSO,we use the significant wave height and wind speed to fit the LASSO model.Hence,the applied model has only 3 parameters,corresponding to the two inputs and a bias.Experimental results on the data of JASON-2,JASON-3,T/P and HY-2 radar altimetry show that LASSO performs better than geophysical data records(GDR) and ordinary least squares(OLS) estimator.Moreover,from the running time,we can see that LASSO is very efficient.

RESEARCH ON MOTION COMPENSATION FOR HIGH RESOLUTION PROFILE OF EXTENDED TARGET FOR STEPPED CHIRP RADAR AND DSP IMPLEMENTATION

A discrete model is set up for High Resolution Range Profile (HRRP) of an extended target and the model of echo from an extended target for a Stepped Chirp Radar (SCR) is proposed. The effect of target motion on a range profile is thoroughly analyzed, and based on which precision re- quirement is developed for motion compensation. By studying the time domain correlation and the rule based on the least burst error, a motion compensation algorithm which satisfies the project requirement is presented, and the cyber-emulation confirms the conclusion. At last the processor is designed by using DSP devices to realize motion compensation and target recognition.

RADAR HRRP RECOGNITION BASED ON THE MINIMUM KULLBACK-LEIBLER DISTANCE CRITERION

To relax the target aspect sensitivity and use more statistical information of the High Range Resolution Profiles (HRRPs), in this paper, the average range profile and the variance range profile are extracted together as the feature vectors for both training data and test data representa-tion. And a decision rule is established for Automatic Target Recognition (ATR) based on the mini-mum Kullback-Leibler Distance (KLD) criterion. The recognition performance of the proposed method is comparable with that of Adaptive Gaussian Classifier (AGC) with multiple test HRRPs, but the proposed method is much more computational efficient. Experimental results based on the measured data show that the minimum KLD classifier is effective.

Sleeve gastrectomy prevents lipoprotein receptor-1 expression in aortas of obese rats

AIM: To investigate the effects of sleeve gastrectomy on adipose tissue infiltration and lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) expression in rat aortas. METHODS: Twenty-four rats were randomized into three groups: normal chow (control), high fat diet (HD) and high fat diet with sleeve gastrectomy (SG). After surgery, the HD and SG groups were fed a high fat diet. Animals were sacrificed and plasma high density lipoprotein (HDL) and low density lipoprotein (LDL) levels were determined. LOX-1 protein and LOX-1 mRNA expression was also measured. Aortas were stained with Nile red to visualize adipose tissue. RESULT: Body weights were higher in the HD group compared to the other groups. HDL levels in control,HD, and SG groups were 32.9 ± 6.2 mg/dL, 43.4 ± 4.0 mg/dL and 37.5 ± 4.3 mg/dL, respectively. LDL levels in control, HD, and SG groups were 31.8 ± 4.5 mg/dL, 53.3 ± 5.1 mg/dL and 40.5 ± 3.7 mg/dL, respectively. LOX-1 protein and LOX-1 mRNA expression was greater in the HD group versus the other groups. Staining for adipose tissue in aortas was greater in the HD group in comparison to the other groups. Thus, a high fat diet elevates LOX-1 protein and mRNA expression in aorta. CONCLUSION: Sleeve gastrectomy decreases plasma LDL levels, and downregulates LOX-1 protein and mRNA expression.