互承结构造型漆艺首饰设计实践——以《鲤》系列首饰设计为例

当前漆艺首饰设计在造型方面往往较为单一重复,从造型角度出发,充分调研市场上各类漆艺首饰的造型结构,同时将广泛应用于建筑领域的互承结构引入漆艺首饰设计实践当中,以对漆艺首饰的单一结构进行造型方面的设计改良。通过运用3D打印这一新兴技术,尝试将传统的工艺美术与新兴科技进行结合,以期在漆艺首饰的造型方面及工艺美术与科技结合方面进行探索性的设计实践。

“鲤”“鳣”考辨

传统训诂大多认为"鲤"和"鳣"是同一种鱼。其实不然。"鲤"是今鲤鱼,"鳣"是今中华鲟。这种错误的根源在于误读《尔雅·释鱼》"鲤鳣鳢鲩鰋鲇"条。

网络热词——“锦鲤”初探

"锦鲤"本指色彩斑斓的鲤鱼,是个旧词,而作为网络热词,它在旧词基础上衍生新的语义,泛指"一切与好运相关的人或物"。文章结合社交网络使用的语料,从"锦鲤"的用法、结构功能、新义衍生机制和走红原因等方面进行了探析。

《鲤·我去二〇〇〇年》

出版社:民主与建设出版社在世界正准备去往2020年的时刻,我们在《鲤·我去二年》中潜入上世纪90年代的回忆,这也是80后作家们给予逐渐远去的少年时代的一次集体性省察,试图共同追溯当时的经验如何影响了现在的自己。

Searching for Protein-coding Genes Using Microsatellites in Common Carp by Comparing to Zebrafish EST Database

In this study, an in silico approach was utilized to identify homologies existing between common carp microsatellite sequences and GenBank database using Blastn and Blastx searches. About 875 microsatellite sites with flanking sequences over 50bp of common carp were first compared to the zebrafish EST database. The results showed that 121 homologies were found using Blastn. Subsequent Blastx searches confirmed 94 sites recorded in the protein database. Except for 33 hypothetical proteins and three unknown proteins, seven out of 58 characterized proteins have been mapped to two linkage maps. In addition, two polymorphic STS markers were developed using matched zebrafish EST sequences by PCR-SSCP method, of which one marker HLJZe33 was mapped successfully. This study was a pilot for comparative studies between common carp and zebrafish, and the results demonstrated that more genetic and genomic resources of zebrafish can be used for the genome research of common carp.

Microsatellite DNA Marker Analysis of Genetic Diversity in Wild Common Carp (Cyprinus carpio L.) Populations

Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and number of effective alleles （Ae） were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package （version 3.63）. There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1） the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2） the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution.

A New Species of the Genus Garra from Nujiang River Basin, Yunnan, China (Teleostei: Cyprinidae)

A new species of the subfamily Labeoninae, Garra nujiangensis Chen and Yang, sp. nov. is recognized from a secondary branch of Nujiang River （upper Salween） in Zhenkang county, Yunnan province, China. It can be distinguished from other beardless Garra species by the following combination of characters： 12 - 14 circumpedunele scales; 48 - 50 lateral- line scales; a pair of rudimentary rostral barbels present in little individuals; 8 - 9 branched dorsal-fin rays; 5 - 6 scales between dorsal-fin origin and lateral line and 3 - 4 scales between pelvic-fin origin and lateral line; caudal peduncle relatively stouter; length between snout tip to posterior margin of eye longer than body depth at anal fin origin; head length longer than body depth at dorsal fin origin; prepelvic length 50.0% - 53.7% of standard length; pre-anus length 58.9% - 63.9% of standard length; body width 16.7% - 19.6% of standard length; body depth at dorsal fin 19.2% - 22.6% of standard length. The present study also verified that the presence of tiny barbells is a juvenile character in some beardless Garra species, such as G. nujiangensis and G. alticorpora.

Electrophoretic Analysis of Isozymes and Discussion about Species Differentiation in Three Species of Genus Gymnocypris

By using the method of electrophoresis,three isozymes (lactate dehydrogenase,malate dehydrogenase and esterase) of three species of genus Gymnocypris were described and analyzed from North Tibet in this paper. The results showed that all three isozymes presented interspecific difference and distinct differentiation among individuals in the same population,and there was no electrophorectic difference between males and females. Analysis of relationships among three naked carps indicated a high degree of similarity between G. selincuoensis and G. cuoensis ,whereas low degree between G. selincuoensis and G. namensis . Furthermore,three isozymes presented expression of null alleles,and the duplicate genes of LDH A 2,LDH B 2,s MDH A 2 and m MDH B 2 also expressed in some individuals. Compared to other tetraploid fishes,three naked carps retained more functional duplicate genes and null alleles. This suggests fishes of genus Gymnocypris are at the early stage of evolution after polyploidization than that of fishes of Catostomidae,it directly related to the later time of schizothoracine fishes originate as well as severe environment.

Cladistic Analysis of the Cyprinid Subgenus Cyprinus (Mesocyprinus) Fang (Teleostei: Cyprinidae)

Phylogeny of the cyprinid subgenus Cyprinus (Mesocyprinus ) is elucidated based on both external and osteological features. All five species of Cyprinus (Mesocyprinus ) are including in the ingroup in addition to two Cyprinus (Cyprinus ) species. Procypris merus was treated as outgroup in comparison. A total of 48 characters were identified as variable among ingroup and outgroup. Phylogenetic analysis was accomplished using Parsimony and Bootstrap methods with Branch and Bound search of PAUP *. There are 28 characters remaining useful after excluding unpolarized characters and autapomorphies of terminal taxa. Analysis of the characters resulted in one tree with a length of 69 steps, Consistency Index (CI) of 0 7246, CI excluding uninformative characters of 0 6122, and Retention Index (RI) of 0 6346. It is apparent that the Mesocyprinus group consisting of five species is a polyphyletic group. This result suggests that subgenus Cyprinus (Mesocyprinus ) should be suspended.

Comparative Study on Muscle Fiber Characteristics and Muscle Nutritional Quality between Two Groups of Yellow River Carps

The aim was to protect the Henan Yellow River carp germplasm resources and provide a scientific basis for the meat quality improvement of Yellow River carps. With artificially farmed and wild Henan Yellow River carps as the research objects, comparative study on muscle fiber diameter and density, routine nutritional composition, calcium and phosphorus contents and amino acids composition was conducted between the two groups of carps. The results showed that the moisture content was significantly higher （P 〈0.05）, the crude fat and crude protein contents were significantly lower （P〈0.05）, the essential amino acids and total amino acids contents were lower （P〉0.05）, the phosphorus content was higher （P 〉0.05）, and the calcium content was lower （P〉0.05） in the wild group compared with those in the farming group. The analysis of muscle fiber characteristics showed that there were significant differences in the average muscle fiber diameter （P〈0.05） and muscle fiber density （P〈0.01） between the two groups of Henan Yellow River carps.

徒劳美学与受困的心——张悦然访谈录

张悦然是21世纪初登场的“80后”作家的标志性人物。当年那场由图书市场与媒体共同制造的青春文学热潮早已褪去,作为继续留在写作阵营中的一员,张悦然走过了向严肃文学试验、探索与转型的长路。在持久的自我更新中,张悦然的写作风格和问题目光渐趋复杂与开阔。在访谈中,张悦然分享了对故乡济南、求学地新加坡的空间情感,并从1980年代生人的身份出发,谈到了由她担任主编的《鲤》以及同代人的友情,谈到了年轻一代写作者面对历史,记忆与遗忘之间的复杂理解。最后,张悦然也谈到了近年来的创作对“受困的心灵”的挣扎与自救的关注,在此基础之上,她也逐渐形成了以“徒劳”为悲剧底色的个人美学取向。

Rates and patterns of microsatellite mutations in common carp (Cyprinus carpio L.)

During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.

Indoor Simulation Study on Nutrient Release of Fishponds Sediment

[Objective] The study aimed to research the nutrients release of ponds sediment.[Method] The sediments which from a new pond(A) and an old one (B) these analyses used to carry out indoor experiment under the anaerobic dark condition for researching on nutrient release. The N(nitrogenous) and P(phosphorous) release were analyzed every two days.[Result] At the prophase, the N release in B was bigger than that in A, while the decline sediment release in A was gentle.[Conclusion] There was no accumulation of organic compound during the breeding time. The NH4-N was the main form of N release; and the P release was correlated with N release, while PO4-P was the main form of P release.

Description of a New Subspecies of the Genus Saurogobio Bleeker (1870)

The genus Saurogobio Bleeker (1870) is a group of small sized cyprinids. Saurogobio dabryi Bleeker (1871) is widely distributed in East Asia.On the basis of careful inspection of morphological and meristic characters of specimen collected from Chenghai Lake in Yunnan and the opening river systems including Pearl River,Minjiang River,Yangtze River,Liaohe River and Songhuajiang River,the Chenghai Lake population of Saurogobio dabryi is considered to represent a new subspecies, Saurogobio dabryi chenghaiensis . Saurogobio dabryi chenghaiensis is distinguished by the combination of following characters.Rostral cap developed,covering over upper lip,and lips with developed papillae.Body and caudal peduncle very slender,body depth 12 7%-15 0% of standard length(SL),caudal peduncle depth 5 5%-6 1% of SL and 31 8%-39 1% of caudal peduncle length.A longitudinal dark stripe present along the axis on each side of body and 6-11 stick shaped black spots (its widths being 25%-50% of its lengths respectively) occur along the stripe.Distance from pelvic fin origin to anus is more than 50% of pelvic fin length.The length of the shortest caudal fin ray is less than 50% that of the longest one.The new subspecies occurs only in Chenghai Lake,Yunnan,China.

Fish maneuver analysis system based on sequence images

The aim is to establish an automatic system to analyze the maneuver performance of fish. A high speed camera （1 000 frame/s） is employed to record fast-start maneuver. Three steps are taken to analyze the kinematics： first, the midline in the first image is partitioned into equal interval lengths and the coordinates of all inter segmental points are saved. Secondly, these points coordinates are searched in the next frame with the digital image correlation （DIC） method, then these points are fitted with a spline curve function. Repeat this step until all the midlines are figured out frame by frame. Finally, according to the variety of midlines, the kinematics of the fast-start is calculated. Using this system to test carp C-start, the duration is divided into two stages： stage 1 is defined as the formation of the C shape and stage 2 as the return flip of the tail followed with forward motion. By tracing the middle line, the kinematic parameters of turning rate, centre of mass （CM） turning rate, CM turning radius, etc. are obtained.

Study on the Morphological Differences of Four Carp Populations

[Objective] The aim was to discuss the morphological differences of four carp populations.[Method] Based on traditional morphological data（including countable and measurable characters） and framework data,the morphological differences and phylogenesis of Yellow River carp population（YR）,wild four-naris carp population（WF）,farmed four-naris carp population（FF） and carp population from Suzhou section of Wusong River（WR） were studied by means of multivariate analysis.[Result] There was no significant difference（P〉0.05） in countable characters among four carp populations.Discriminant analysis showed the discriminating accuracy of YR,WF,FF and WR population were 87.5%,78.1%,73.5% and 96.8% respectively,and the synthetic discriminating accuracy of four populations was up to 83.7%.In addition,cluster analysis revealed that YR population and WF population clustered together,while FF population and WR population clustered together.Principal component analysis indicated that the morphological differences among four populations mainly came from the head,tail and vertical axis of fish.[Conclusion] The study could provide theoretical foundations for the purification and rejuvenation of rare four-naris carp.

Study on HSP70 Gene Expression in Different Tissue of Cyprinus carpio

[ Objective] The aim of this study was to investigate whether HSP70 can be used as a stress monitoring indicator in Cypnnus carpio breeding. [Method] Based on HSP70 sequence of Cyprinus carpio （AY120894）, one pair of primers was designed and synthesized, while the total RNA of liver tissues in Cyprinus carpio was extracted. Some cDNA fragments of Cyprinus carpio HSP70 were cloned by RT-PCR, and its differential expression in various tissues such as heart, intestine, mucus, gonad, swim bladder, gill and fin in Cyprinus carpio was also studied. [Result] The cDNA sequence of 480 bp was obtained from Cypdnus carpio HSPTO gene by RT-PCR amplification. Homology comparison between the deduced amino acid sequence after sequencing and that of other types of fish showed that the homology among Cyprinus carpio, Danio rerio, Ohcorhynehus mylciss, Paralichthys olivaceus, Xiphophoorus maculates and Carassius auratus was 96%, 98%, 98%, 96%, 98% and 96% respectively. The expression of HSP70 was detected in eight tissues of Cypnnus carpio. The expression was the highest in heart, followed by swim bladder and fin, but there was no significant difference between them （ P 〉 0.05 ）. There was no significant difference among the ex- pression in three tissues of intestine, mucus and fat （ P〉0.05）, but their expression was significantly higher than those in gonad and gill （ P〈 0.05）. [ Conclusion] HSPTO gene expression is a suitable criterion for monitoring the stress degree, stress capacity and healthy conditions in Cyprinus carpio breeding.

cGH Gene Transfer and PCR Detection in Crucian Carp

By inserting the CMV with HSV TK promoter into the plasmid pBlue-cGH which contained cGH gene, a chimeric expression vector was constructed for transgenic fish. After the vector was transferred into fertilized eggs of crucian carp by means of microinjection, the transgenic crucian carp was obtained. The result of PCR detection showed that the growth hormone gene of the exogenous carp was integrated into the genome of crucian carp, and the transgenic crucian carp displayed enhancement of its growth.

A Novel Conservation Structure Found in Vertebrate Mitochondrial tRNA￣(phe) Gene

The nucleotide scquence of tRNAphe gene of Carp mitochondria was determined. Sequence comparisons made among Whale,Human,Xenopus laevis, Bovine, Mouse,Chicken and Carp revealed a novel conservative structure in the D. stem (dihydrouridine stem),which is known to vary in other vertebrate mitochondrial and eytoplasmic tRNA genes.This conservative structure contains 13 bp. When the first 7 bp of the conserviative structure were compared with the A domain recognized by RNA Pol III, we noticed partial homology between these two kinds of sequences among different species. In view of the tRNAphe gene's close position to the D loop,it is reasonable to expect extraordinary functions in this novel conservation structure.

Availability Test of the Free Phospholipids in the Plasma of Three Species of Freshwater Fish

[Objective] The research aimed to determine the existence of free phospholipids in the plasma of three species of freshwater fish and lay the foundation for discussing the physiological mechanism of coagulation phenomena in three species of freshwater fish.[Method] The activity of heated phospholipids was detected by the availability test of heated rabbit cephalin.Small amount of the platelet-poor plasma of the rabbit Oryctolagus cuniculus,the cell-free plasma of the carp Cyprinus carpio,the crucian Carassi...