A total of 206 SSR （Simple Sequence Repeats） primer pairs were used to detect genetic diversity in 52 accessions of three unique wheat varieties of western China. A total of 488, 472, and 308 allelic variants were d...A total of 206 SSR （Simple Sequence Repeats） primer pairs were used to detect genetic diversity in 52 accessions of three unique wheat varieties of western China. A total of 488, 472, and 308 allelic variants were detected in 31 Yunnan, 15 Tibetan and 6 Xinjiang wheat accessions with an average of PIC values 0.2764, 0.3082, and 0.1944, respectively. Substantial differences in allelic polymorphisms were detected by SSR markers in all the 21 chromosomes, the 7 homoeologous groups, and the three genomes （A, B, and D） in Yunnan, Tibetan, and Xinjiang wheat. The highest and lowest allelic polymorphisms in all the 21 chromosomes were observed in 3B and 1D chromosomes, respectively. The lowest and highest allelic polymorphisms among the seven homoeologous groups was observed in 6 and 3 homoeologous groups, respectively. Among the three genomes, B genome showed the highest, A the intermediate, and D the lowest allelic polymorphism. The genetic distance （GD） indexes within Yunnan, Tibetan, and Xinjiang wheat, and between different wheat types were calculated. The GD value was found to be much higher within Yunnan and Tibetan wheat than within Xinjiang wheat, but the GD value between Yunnan and Tibetan wheat was lower than those between Yunnan and Xinjiang wheat, and between Tibetan and Xinjiang wheat. The cluster analysis indicated a closer relationship between Yunnan and Tibetan wheat than that between Yunnan and Xinjiang wheat or between Tibetan and Xinjiang wheat.展开更多
Genetic diversity at Gli_1, Gli_2 and Glu_1 loci was investigated in 32 accessions of Chinese endemic wheat by using acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulfate (SDS)_PAGE. There were 8 ...Genetic diversity at Gli_1, Gli_2 and Glu_1 loci was investigated in 32 accessions of Chinese endemic wheat by using acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulfate (SDS)_PAGE. There were 8 gliadin and 3 high_molecular_weight (HMW)_glutenin patterns in 14 Yunnan hulled wheat ( Triticum aestivum ssp. yunnanese King) accessions, 9 gliadin and 4 HMW_glutenin patterns in 9 Tibetan weedrace ( T. aestivum ssp. tibetanum Shao ) accessions, and 9 gliadin and 5 HMW_glutenin patterns in 9 Xinjiang rice wheat ( T. petropavlovskyi Udacz. et Migusch.) accessions. One accession (i.e. Daomai 2) carried new subunits 2.1+10.1 encoded by Glu_D1. Among the three Chinese endemic wheat groups, a total of 10, 14 and 11 alleles at Gli_1 locus; 11, 14 and 12 alleles at Gli_2 locus; and 5, 6 and 8 alleles at Glu_1 locus were identified, respectively. Among Yunnan hulled wheat, Tibetan weedrace and Xinjiang rice wheat, the Nei's genetic variation indexes were 0.3798, 0.5625 and 0.5693, respectively. These results suggested that Tibetan weedrace and Xinjiang rice wheat had higher genetic diversity than Yunnan hulled wheat.展开更多
The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ...The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ( Hordeum vulgare L.) mapping. Out of the 22 STS_PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with Hin fⅠ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32 H. bogdanii accessions, a total of 315 bands were observed in 88 STS_PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty_three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS_PCR_based genetic diversity index ( GD ) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the genetic relationships between accessions was constructed using the unweighted pair_group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguished by STS_PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution.展开更多
基金Hi-Tech Research and Development (863) Program of China (No. 2006AA10Z1F6)Hi-Tech Re-search of Jiangsu Province (No.BG2005310)+2 种基金the Program for Changjiang Scholars and Innovative Research Team in University (No.10418) (PCSIRT)Innovation Foundation of Young Science and Technology of Nanjing Agriculture UniversityIntroduction of Talents Foundation of Nanjing Agriculture University.
文摘A total of 206 SSR （Simple Sequence Repeats） primer pairs were used to detect genetic diversity in 52 accessions of three unique wheat varieties of western China. A total of 488, 472, and 308 allelic variants were detected in 31 Yunnan, 15 Tibetan and 6 Xinjiang wheat accessions with an average of PIC values 0.2764, 0.3082, and 0.1944, respectively. Substantial differences in allelic polymorphisms were detected by SSR markers in all the 21 chromosomes, the 7 homoeologous groups, and the three genomes （A, B, and D） in Yunnan, Tibetan, and Xinjiang wheat. The highest and lowest allelic polymorphisms in all the 21 chromosomes were observed in 3B and 1D chromosomes, respectively. The lowest and highest allelic polymorphisms among the seven homoeologous groups was observed in 6 and 3 homoeologous groups, respectively. Among the three genomes, B genome showed the highest, A the intermediate, and D the lowest allelic polymorphism. The genetic distance （GD） indexes within Yunnan, Tibetan, and Xinjiang wheat, and between different wheat types were calculated. The GD value was found to be much higher within Yunnan and Tibetan wheat than within Xinjiang wheat, but the GD value between Yunnan and Tibetan wheat was lower than those between Yunnan and Xinjiang wheat, and between Tibetan and Xinjiang wheat. The cluster analysis indicated a closer relationship between Yunnan and Tibetan wheat than that between Yunnan and Xinjiang wheat or between Tibetan and Xinjiang wheat.
文摘Genetic diversity at Gli_1, Gli_2 and Glu_1 loci was investigated in 32 accessions of Chinese endemic wheat by using acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulfate (SDS)_PAGE. There were 8 gliadin and 3 high_molecular_weight (HMW)_glutenin patterns in 14 Yunnan hulled wheat ( Triticum aestivum ssp. yunnanese King) accessions, 9 gliadin and 4 HMW_glutenin patterns in 9 Tibetan weedrace ( T. aestivum ssp. tibetanum Shao ) accessions, and 9 gliadin and 5 HMW_glutenin patterns in 9 Xinjiang rice wheat ( T. petropavlovskyi Udacz. et Migusch.) accessions. One accession (i.e. Daomai 2) carried new subunits 2.1+10.1 encoded by Glu_D1. Among the three Chinese endemic wheat groups, a total of 10, 14 and 11 alleles at Gli_1 locus; 11, 14 and 12 alleles at Gli_2 locus; and 5, 6 and 8 alleles at Glu_1 locus were identified, respectively. Among Yunnan hulled wheat, Tibetan weedrace and Xinjiang rice wheat, the Nei's genetic variation indexes were 0.3798, 0.5625 and 0.5693, respectively. These results suggested that Tibetan weedrace and Xinjiang rice wheat had higher genetic diversity than Yunnan hulled wheat.
文摘The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ( Hordeum vulgare L.) mapping. Out of the 22 STS_PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with Hin fⅠ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32 H. bogdanii accessions, a total of 315 bands were observed in 88 STS_PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty_three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS_PCR_based genetic diversity index ( GD ) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the genetic relationships between accessions was constructed using the unweighted pair_group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguished by STS_PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution.
