In order to investigate the regulatory role of connective tissue growth factor （CTGF） on production of RANTES （regulated on activation, normal T cell expressed and secreted） in rat glomerular mesangial cells, and ...In order to investigate the regulatory role of connective tissue growth factor （CTGF） on production of RANTES （regulated on activation, normal T cell expressed and secreted） in rat glomerular mesangial cells, and the modulatory effect of lipoxin A4 （ LXA4 ） on action of CTGF, and to explore the mechanisms of action of CTGF and LXA4, cultured rat mesangial cells were treated with CTGF, with or without preincubation with LXA4. Expression of mRNA was analyzed by RT-PCR. Protein of RANTES in the supematants was determined by ELISA. Monocyte transmigration was assessed by in vitro chemotaxis assay. Expression of p42/44 mitogen-activated protein kinase （MAPK）, phosphoinositide 3-kinase （ PI3- K） and protein kinase B （PKB） was assessed by Western blotting. DNA-binding activity of nuclear factor-roB （NF-kB） was determined by electrophoretic mobility shift assay （EMSA）. To observe whether transfection of LXA4 receptor homologue gene （LRHg） into mesangial cells intensified these modulatory effects of LXA4, mesangial cells were transfected with pcDNA3.1/LRHG vector. The results showed that CTGF enhanced the mRNA expression and protein release of RANTES, and the expression of phospho （P）-p42/44 MAPK, P-PI3-K, P-PKB and NF-kB. P-p42/44 MAPK blockade inhibited the CTgF-induced expression of P-p42/44 MAPK and partially decreased the level of RANTES in supematants. P- PI3-K blockade downregulated the CTGF-stimulated expression of P-PI3-K, P-PKB and NF-kB, and partially decreased the release of RANTES. NF-kB blockade abrogated the CTGF-activated NF-kB and partially decreased the secretion of RANTES. LXA4 dose-dependently inhibited the CTGF-stimulated above action. Transfection of LRHG into mesangial cells intensified these inhibitory effects of LXA4 on CTGFinduced release of RANTES and expression of the P-p42/44 MAPK. In conclusion, LXA4 inhibits CTGFinduced production of RANTES via PI3-K/PKB/NF-kB and p42/44 MAPK-dependent signal pathway, which is mediated by LRHG in rat mesangial cells.展开更多
The P_(k)-path graph P_(k)(G)corresponding to a graph G has for vertices the set of all paths of length k in G.Two vertices are joined by an edge if and only if the intersection of the corresponding paths forms a path...The P_(k)-path graph P_(k)(G)corresponding to a graph G has for vertices the set of all paths of length k in G.Two vertices are joined by an edge if and only if the intersection of the corresponding paths forms a path of length k-1 in G,and their union forms either a cycle or a path of length k+1.Let Ek={(v,p),p E V(P_(k)(G)),v is an end vertex of p in G},we define total P_(k)-graphs T_(k)(G)as Yk(G)=(V(G)UV(P_(k)(G)),E(G)U E(PI(G))U Ek).In this note,we introduce total P,-graphs Th(G)and study their edge connectivity,as the generaliza-tion of total graphs.展开更多
Let G be a graph, an independent set Y in G is called an essential independent set (or essential set for simplicity), if there is {y 1,y 2} Y such that dist (y 1,y 2)=2. In this paper, we wi...Let G be a graph, an independent set Y in G is called an essential independent set (or essential set for simplicity), if there is {y 1,y 2} Y such that dist (y 1,y 2)=2. In this paper, we will use the technique of the vertex insertion on l connected ( l=k or k+1,k≥2 ) claw free graphs to provide a unified proof for G to be hamiltonian or 1 hamiltonian, the sufficient conditions are expressed by the inequality concerning ∑ki=0N(Y i) and n(Y) for each essential set Y={y 0,y 1,...,y k} of G , where Y i={y i,y i-1 ,...,y i-(b-1) }Y for i∈{0,1,...,k} (the subscriptions of y j ’s will be taken modulo k+1 ), b ( 0【b【k+1 ) is an integer, and n(Y)={v∈V(G): dist (v,Y)≤2 }.展开更多
文摘In order to investigate the regulatory role of connective tissue growth factor （CTGF） on production of RANTES （regulated on activation, normal T cell expressed and secreted） in rat glomerular mesangial cells, and the modulatory effect of lipoxin A4 （ LXA4 ） on action of CTGF, and to explore the mechanisms of action of CTGF and LXA4, cultured rat mesangial cells were treated with CTGF, with or without preincubation with LXA4. Expression of mRNA was analyzed by RT-PCR. Protein of RANTES in the supematants was determined by ELISA. Monocyte transmigration was assessed by in vitro chemotaxis assay. Expression of p42/44 mitogen-activated protein kinase （MAPK）, phosphoinositide 3-kinase （ PI3- K） and protein kinase B （PKB） was assessed by Western blotting. DNA-binding activity of nuclear factor-roB （NF-kB） was determined by electrophoretic mobility shift assay （EMSA）. To observe whether transfection of LXA4 receptor homologue gene （LRHg） into mesangial cells intensified these modulatory effects of LXA4, mesangial cells were transfected with pcDNA3.1/LRHG vector. The results showed that CTGF enhanced the mRNA expression and protein release of RANTES, and the expression of phospho （P）-p42/44 MAPK, P-PI3-K, P-PKB and NF-kB. P-p42/44 MAPK blockade inhibited the CTgF-induced expression of P-p42/44 MAPK and partially decreased the level of RANTES in supematants. P- PI3-K blockade downregulated the CTGF-stimulated expression of P-PI3-K, P-PKB and NF-kB, and partially decreased the release of RANTES. NF-kB blockade abrogated the CTGF-activated NF-kB and partially decreased the secretion of RANTES. LXA4 dose-dependently inhibited the CTGF-stimulated above action. Transfection of LRHG into mesangial cells intensified these inhibitory effects of LXA4 on CTGFinduced release of RANTES and expression of the P-p42/44 MAPK. In conclusion, LXA4 inhibits CTGFinduced production of RANTES via PI3-K/PKB/NF-kB and p42/44 MAPK-dependent signal pathway, which is mediated by LRHG in rat mesangial cells.
基金supported by Natural Sciences Foundation of Guangxi Province(2012GXNSFBA053005)
文摘The P_(k)-path graph P_(k)(G)corresponding to a graph G has for vertices the set of all paths of length k in G.Two vertices are joined by an edge if and only if the intersection of the corresponding paths forms a path of length k-1 in G,and their union forms either a cycle or a path of length k+1.Let Ek={(v,p),p E V(P_(k)(G)),v is an end vertex of p in G},we define total P_(k)-graphs T_(k)(G)as Yk(G)=(V(G)UV(P_(k)(G)),E(G)U E(PI(G))U Ek).In this note,we introduce total P,-graphs Th(G)and study their edge connectivity,as the generaliza-tion of total graphs.
文摘Let G be a graph, an independent set Y in G is called an essential independent set (or essential set for simplicity), if there is {y 1,y 2} Y such that dist (y 1,y 2)=2. In this paper, we will use the technique of the vertex insertion on l connected ( l=k or k+1,k≥2 ) claw free graphs to provide a unified proof for G to be hamiltonian or 1 hamiltonian, the sufficient conditions are expressed by the inequality concerning ∑ki=0N(Y i) and n(Y) for each essential set Y={y 0,y 1,...,y k} of G , where Y i={y i,y i-1 ,...,y i-(b-1) }Y for i∈{0,1,...,k} (the subscriptions of y j ’s will be taken modulo k+1 ), b ( 0【b【k+1 ) is an integer, and n(Y)={v∈V(G): dist (v,Y)≤2 }.
