LightWave 3D作品「贝壳」制作全记录

这是一篇比较基础的LightWave教程，作者对一幅完事的作品的主要制作步骤进行了细致的讲解，特别是材质部分，读者如能举一反三，相信会创作出更为出色的作品。

A Preliminary Analysis on the Construction and Expression of Eukaryotic Expression Vectors of Mytilin and Myticin from Mytilus coruscus

[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides （mytilin and myticin） from Mytilus coruscus.[Method] By the screening of antibacterial peptide genes of mytilin and myticin of Mytilus coruscus,five antibacterial peptide genes were selected.Then,the relative eukaryotic expressing vectors were constructed by the use of PCR technique and DNA recombinant technology.Subsequently,they were transferred in to S78 Saccharomyces cerevisia by using LiAC transformation method,and then preliminary expressing analysis was carried out.[Result] Five eukaryotic expressing vectors of antibacterial peptides from Mytilus coruscus were successfully constructed,and the results of mRNA detection revealed that the five antibacterial peptides from Mytilus coruscus were successfully transcribed.[Conclusion] The results provide a basis for using genetic engineering to express antibacterial peptides of mytilin and myticin from Mytilus coruscus,and for developing the further study of antibacterial peptides from Mytilus coruscus based on this.

Two New ent-Kauranoids from Isodon tenuifolia

Two new ent-kauranoids, tenuifolin A (3beta,6alpha, 15beta-trihydroxy-1alpha, 7beta-diacetoxy-11beta, 16beta-epoxy-ent-kaurane) (1) and tenuifolin B (1alpha,6alpha, 11beta-trihydroxy-3beta,7beta-diacetoxy-ent-kaur-16-en-15-one) (2), together with four known compounds were isolated from the aerial parts of Isodon tenuifolia (W. W. Smith) Kudo collected from Zhongdian County, Yunnan Province, China. Their structures were determined by the spectral methods (including 2D NMR techniques).

Genetic Analysis of Cultured and Wild Populations of Mytilus coruscus Based on Mitochondrial DNA

DNA sequences from the mitochondrial gene cytochrome oxidase subunit I （mtDNA CO I） were used to estimate the genetic variability in two wild populations and two cultured populations of the hard shelled mussel, Mytilus coruscus. Thirty haplotypes were identified in the four populations. The cultured populations exhibited a lower number of haplotypes and genetic diversity than those of the wild populations, suggesting that a small number of effective founding breeders contributed to the genetic variation of the cultured populations. No significant differentiation was observed between the cultured population and local wild population, implying that persistent gene flow occurred in these populations. This genetic survey is intended as a baseline for future genetic monitoring of M. coruseus aquaculture stocks.

A New Sampling Approach to Obtain Genomic DNA of Marine Shellfish

[Objective] The aim was to explore a new sampling approach to obtain genomic DNA of marine shellfish,as well as to provide reference for the molecular biology research on precious shellfish.[Method] Meretrix meretrix,Atrina pectinata,Perna viridis,Crassostrea hongkongensis and Scapharca kagoshimensis were used as experimental materials and the genomic DNA of adductor muscle was taken as reference to extract the genomic DNA of shell cavity fluids with the conventional phenol-chloroform method.And then biophotometer,agarose gel electrophoresis,amplification and sequencing of the target fragments were used to examine the quality of genomic DNA.At the same time,the phylogenic tree was constructed to verify the reality of source contributions.[Result] The quality of genomic DNA of shell cavity fluids extracted by the phenol-chloroform method was better than the genomic of adductor muscle.The genomic DNA extracted by this method showed less content of protein,polyphenol and pigment,which could completely meet the demands of amplification and sequencing of the target fragments.Through the phylogenic tree,it was verified that the source contributions of shell cavity fluids were not come from foreign pollutions.[Conclusion] It is completely feasible to obtain the genomic DNA from shell cavity fluids,which could be applied in the target fragments amplification.

ent-Kaurene Diterpenoids from Isodon adenanthus

Three new ent-kaurene diterpenoids, adenanthins N, 0 and P (1-3), and four known diterpenoids, leucophyllin E (4), glabcensin C (5), adenanthin A (6), leucophyllin B (7), together with a highly unsaturated fatty acid, 9,16-dioxo-10,1 2,14-octadeca-trienoic acid (8), were isolated from Isodon adenanthus (Diels) Kudo. The structure of compound 4 was revised accordingly. Compound I showed significant cytotoxic activity against K562 cells with IC50 = 0.45 mug/mL.

Comparison of growth and survival of larvae among different shell color stocks of bay scallop Argopecten irradians irradians(Lamarck 1819)

In order to improve production and breed new broods of bay scallop Argopecten irradians irradians, different-colored orange, purple and white lines were established by two mating methods of self-fertilization and mass spawning at a commercial scallop hatchery in spring, 2002. And then larval growth and survival of different lines was compared to test whether there is a relationship between shell color and growth and survival at early developmental stage. Both growth and survival have no significant differences among different experimental larvae of self-fertilization or mass spawning. Results are as following in the order of orange, purple and white shell stock. For the self-fertilization, growth rates of larvae were 6.174, 6.412, and 6.599 μm/d, respectively. Survival rates of larvae at Day 3 were 74.41%, 76.86%, and 82.05%; Day 6 were 49.14%, 65.63%, and 52.79%; and Day 9 were 25.06%, 20.80%, and 26.47%, respectively. For the mass spawning, the growth rates were 7.836, 7.941, and 7.878 μm/d, respectively. Survival rates at Day 3 were 93.05%, 91.95%, and 92.50%; Day 6 were 79.17%, 78.05%, and 82.50%; and Day 9 were 34.72%, 36.67%, and 38.33%, respectively. The absence of any relationship between shell color and growth and survival at the larval stage may be resulted from their common genetic basis.

Significant role of carbonate radicals in tetracycline hydrochloride degradation based on solar light-driven TiO2-seashell composites:Removal and transformation pathways

TiO2-seashell composites prepared via a sol-gel method were used to generate carbonate radicals(·CO3–) under solar light irradiation. ·CO3–, a selective radical, was employed to degrade the target tetracycline hydrochloride contaminant. A series of characterizations was carried out to study the structure and composition of the synthesized TiO2-seashell composite. This material exhibits excellent solar light-driven photochemical activity in the decomposition of tetracycline hydrochloride. The possible pathway and mechanism for the photodegradation process were proposed on the basis of high-resolution electrospray ionization time-of-flight mass spectrometry experiments. Finally, we investigated the reusability of the TiO2-seashell composite. This study is expected to provide a new facile pathway for the application of ·CO3– radicals to degrade special organic pollutants in water.

Concentrations and distribution of organochlorine pesticides in shellfish from Changjiang estuary

To analyze and evaluate the status of organochlorine pollutants in the Changjiang （Yangtze River） estuary and adjacent waters, the concentrations of hexachlorocyclohexane （HCHs） and dichlorodiphenyltrichloroethane （DDTs） in shellfish collected in study area from 2006 to 2007 were determined with gas chromatography （GC）. The concentration range of HCHs was （ND-12.13）×10^-3 mg/kg wet weight and averaged at 0.54×10^-3 mg/kg while the concentration of DDTs was in the range of （4.06-281.73） ×10^-3 mg/kg with a mean of 57.52×10^-3 mg/kg in the survey areas. The concentrations of DDTs in the shellfish were higher than HCHs＇, so that DDTs could be considered as typical organochlorine pollutants in the areas. The concentrations of DDTs in the shellfish were higher than HCHs＇, so that DDTs could be considered as typical organochlorines pollutants. The HCHs in all the shellfish conformed to the first level of criterion （0.02 mg/kg） of the Marion Biology Quality （GB 18421-2001）, and that of DDTs in most samples were beyond the first level （0.01 mg/kg） but conformed to the second level （0.10 mg/kg）. On average, α-HCH and δ-HCH occupied the most part of HCHs, while O,P＇-DDT and P,P＇-DDT occupied the most part of DDTs. The concentrations of organocholorine pesticides in shellfish samples varied in site and in species. The highest level occurred at the Shengsi （SS）, followed by Yangkougang （YKG）, Lvsi （LS）, Dongyuan （DY） and Beibayao （BBY）, low concentrations were observed at Changsha （CS）, Beidaodi （BDD）, and Gouqi （GQ）. The concentration of HCHs and DDTs in most sites decreased clearly from 2006 to 2007 except for YKG, DY, BDD, LYS, and SS. All of above results suggested that the study area was slightly affected by organochlorine pesticide, special by DDTs.

De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop Argopecten irradians

Molluscan shell color has received persistent attention for its distinctive diversity and complexity. In the present study, six transcriptome libraries obtained from two developmental stages, pre-pigmentation and post-pigmentation, were used for paired-end sequencing in the bay scallop Argopecten irradians. In total, 289 839 646 paired-end reads were assembled into 70 929 transcripts. Using BLASTX and BLASTN, 30 896 unigenes were successfully annotated against the SWISS-PROT, NR, and KOG database. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes classification identified numbers of unigenes involved in biomineralization and pigmentation. Digital gene expression analysis revealed that melanin, trace metal elements and porphyrins are potentially involved in shell coloration of A. irradians.

Influences of Oyster Shell Soil Conditioner on Soil and Plant Rhizospheric Microorganisms

Oyster shell soil conditioner had significant influence on soil and rhizospheric microorganisms in their biomass,respiratory intensity and nutritional requirement. It could stimulate growth of soil and rhizospheric microorganisms, especially nitrogen-fixers, and intensify soil respiration in proportion to the dose and fertilizing time of the conditioner, leading to the increase in the number of nitrogen fixing bacteria and the decrease in the number of bacteria with special nutrition demands.

Importance of kelp-derived organic carbon to the scallop Chlamys farreri in an integrated multi-trophic aquaculture system

Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture （IMTA） systems. A beneficial relationship between seaweed and bivalve in the seaweed- based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharinajaponica to the co-cultured scallop Chlamysfarreri in a typical IMTA farm in Sungo Bay （Weihai, North China）. The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 ~tg/L, which was 25.6% to 73.8% of total particulate organic carbon （TPOC） during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water （F=0.993, P=0.003）. Evaluation showed that for 17 000 tons （wet weight） of annual scallop production, the kelp contributed about 139.3 tons of carbon （535.8 tons of dry mass）. This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.

REPORT ON PONTONIINE SHRIMPS (CRUSTACEA: DECAPODA) COLLECTED BY JOINT CHINESE-GERMAN MARINE BIOLOGY EXPEDITIONS TO HAINAN ISLAND,SOUTH CHINA SEA I. ANCHISTUS, CONCHODYTES, CORALLIOCARIS

The present paper reports 7 species of pontoniine shrimps collected from Hainan Island, South China Sea, by the Joint Chinese German Marine Biology Expeditions, in which, Conchodytes nipponensis (De Haan, 1844) and Coralliocaris brevirostris Borradaile, 1898 are new records from Chinese waters.

Proton Orbital[541]1/2 and Backbending in ^(178)W

The microscopic mechanism of nine experimentally observed bands in ^178W is investigated using the particle-number conserving method of the cranked shell model with monopole and quadrupole paring interactions. The experimental results, including the moments of inertia and angular momentum alignments of nine bands in ^178W, are reproduced well by the particle-number conserving calculations, in which no free parameter is involved. Calculations demonstrate that occurrence of sharp backbending comes mainly from the contribution of high-j intruder orbitals vi13/2 or πh11/2 and their interference effect with orbitals near the Fermi surface. Theω variation of the occupation probability of each cranked orbital and the contribution to moment of inertia from each cranked orbital are analyzed.

Bacteriological Analysis of the Digestive Tube of the Mud Snail (Bullacta exarata Philippi) and Its Rearing Shoal

The bacterial flora in the digestive tube of Bullacta exarata Philippi and its rearing shoal were investigated. A total of 157 strains of heterotrophic bacteria, isolated from crop, stomach intestine and other parts of the digestive tube, mainly belong to the genera Photobacterium, Bacillus, Pseudomonas Vibrio and some genera of the family Enterobacteriaceae. There are significantly more varieties of bacteria in crop than in stomach and intestine. A total of 173 strains of bacteria were isolated from the rearing shoal, belonging to 13 genera. The 5 predominant genera, such as Bacillus and Photobacterium, are the same as those in the digestive tube, but greatly differ in percentages. The number of heterotrophic bacteria and Vibrio in rearing shoal change in line with the alteration of the temperature, and are significantly affected by the use of pesticides.

Biomarker responses in the bivalve Chlamys farreri to the water-soluble fraction of crude oil

To investigate the effect of the water soluble fraction of crude oil(WSF) on marine bivalves, the scallop C hlamys farreri was exposed to three WSF concentrations(0.18 mg/L, 0.32 mg/L, and 0.51 mg/L, respectively) in seawater. Petroleum hydrocarbon contents in scallops and a suite of enzymes [7-Ethoxyresorufin-O-deethylase(EROD), aryl hydrocarbon hydroxylase(AHH), glutathione S-transferase(GST), and glutathione peroxidase(GPx)] in gills and digestive glands were monitored over 10 days. The results revealed that WSF affected the activity of the four enzymes in the gills and digestive glands. EROD activity in the gills was significantly induced in most individuals of the three test groups, while in the digestive gland it was significantly induced in the low-concentration group within 4 days but was inhibited in the middle- and high-concentration groups on days 1, 4, and 10. AHH activity in the gills of all treatment groups was significantly induced on day 1. In the digestive gland, AHH activity was induced in most individuals from the treatment groups. In all treatment groups, GST activity was significantly inhibited from days 2 to 10 in the gills and was induced after day 4 in the digestive gland. GPx activity in the gills was significantly inhibited throughout the exposure period in all treatment groups. There was no overall significant difference in GPx activity in the digestive gland between the control and treatment groups. Our results also revealed that petroleum hydrocarbon concentrations in the tissues increased linearly with exposure time. EROD activity in the digestive gland and GST and GPx activity in the gill tissue were negatively correlated with petroleum hydrocarbon body burden. These enzymes play important roles in detoxification and can act as potential biomarkers for monitoring petroleum hydrocarbon contaminants in the marine environment.

Clean and Safe Supply of Fish and Shellfish to Clear the HACCP Regulation by Use of Clean and Cold Deep Ocean Water in Rausu,Hokkaido,Japan

For the supply of fish and shellfish to consumers in fresh condition, clean handling after catch from the sea is essential. According to HACCP （Hazard Analysis and Critical Control Points）, it is important to meet such requirement by keeping fish and shellfish under a certain low temperature and clean conditions after catching. The deep ocean water （DOW） characterized by low temperature and cleanliness has been chosen for fish and shellfish handlings, particularly for salmon, cod, and sea urchin in Town ＇Rausu＇ in Hokkaido, Japan. DOW below 2.9℃ of an amount of nearly 5 000 m^3 is planned to be pumped up every day from a depth of about 350 m, and temporarily stored in a large simulated tank on land. DOW is then supplied to fish boats through hydrants distributed throughout the harbor and used for keeping salmon in clean and cold conditions. Ice made from DOW is also used for lowering temperature if necessary. DOW and ice made from DOW are also used during the transportation of fish and shellfish. The entire system is scheduled to be completed by the summer of 2005.

Nonlinear Analysis of Laminated Shells via Piece-wise Rayleigh-Ritz Technique

Based on Reddy higher-order shear deformation theory, a general refined shell theory suitable to nonlinear analysis of tire structure is developed in this paper. The piece-wise Rayleigh-Ritz procedure and Bezier polynomials are applied to analyze deformations and stress distributions of the multlayered tire subjected to uniform inflation in detail. Furthermore, 3-dimension FEM analysis of laminated tire by standard software ANSYS is adopted to compare with the present model. It is demonstrated that both two solutions are in fairly good agreement.

Use of Mussel Shells as a Soil Amendment:Effects on Bulk and Rhizosphere Soil and Pasture Production

Large quantities of mussel shells （66000-94000 t year^-1）, an alkaline material that can be used as a soil amendment, are generated as waste in Galicia, NW Spain. A field trial was carried out by planting different pasture species in a Haplic Umbrisol using a randomized block design with four blocks and six treatments （not amended control or soil amended with lime, finely ground shell, coarsely ground shell, finely ground calcined shell or coarsely ground calcined shell） to compare the effects of lime and mussel shells additions on a soil with a low cation exchange capacity and high AI saturation. The trial was established in March 2007, and samples of plants and soil were collected when plots were harvested in summer 2008 （separating the bulk and rhizosphere soil）. The soils were analyzed for pH, total C, total N, available P, exchangeable cations, effective cation exchange capacity and available micronutrients. Dry matter yield was measured in all plots and plants were analyzed for nutrients. Application of mussel shells and the commercial lime resulted in an increase in pH and exchangeable Ca and a decrease in exchangeable Al and Al were most noticeable in the rhizosphere. The amendment of Ca in the plant. saturation. The stability of pH over time was high. These effects also had a positive effect on dry matter yield and concentration

Network pharmacology-based strategy to investigate harmacological mechanisms of Isodon serra(Maxim.)Hara for treatment of inflammatory

Widely distributed in plants,ent-kaurane diterpenoids could reduce the incidence of inflammatory.The most important active ingredient of Isodon serra(Maxim.)Hara is ent-kaurane diterpenoids,which contribute to the anti-inflammatory pharmacological effects of Isodon serra.However,the ingredients,the active compounds,drug targets,inflammatory targets and exact molecular mechanism of Isodon serra in treating inflammatory are still unclear.The purpose of this study was to use the method of network pharmacological analysis to find the active compounds in Isodon serra.These active compounds match the library of ent-kaurane diterpenoids compounds we established,and we find all the eligible ent-kaurane diterpenoids compounds.Isodon serra related and anti-inflammatory targets were found and then combined to get intersection,which represented potential anti-inflammatory targets of active compounds in Isodon serra.Moreover,anti-inflammatory targets and active compounds targets protein-protein interaction network were merged to get the protein-protein interaction network intersection and core genes in anti-inflammatory target protein-protein interaction network.For the anti-inflammatory targets of Isodon serra,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis were executed to confirm gene functions of Isodon serra in antagonizing inflammation.Finally,TCMSP analysis identified 10 active compounds out of 48 ent-kaurane.The pathway analysis showed enrichment for different pathways like AGE-RAGE signaling pathway in diabetic complications,small cell lung cancer and human cytomegalovirus infection,which were all connected to inflammatory.On the whole,the proposed method clearly identified the ent-kaurane diterpenoids of Isodon serra and the results gave the active compounds of Isodon serra for the first time.The combining use of the qualitative analysis of traditional Chinese medicine(TCM)and network pharmacological methods could discover potential drug targets and reveal the biological process of TCM,which would open up a new approach in the study of TCM in future.