期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
拟南芥蛋白质组研究中双向电泳技术条件的优化 被引量:9
1
作者 罗泽宇 杨粤军 刘选明 《激光生物学报》 CAS CSCD 2008年第4期539-543,共5页
双向电泳技术是蛋白质组学研究中的关键技术,是目前分辨率最高的工具之一。而提高双向电泳图蛋白质点的数目和分辨率,可以提高蛋白质组技术平台的信息完整性。通过对拟南芥双向电泳技术过程中的适当改进,如蛋白质的提取与溶解方法、上... 双向电泳技术是蛋白质组学研究中的关键技术,是目前分辨率最高的工具之一。而提高双向电泳图蛋白质点的数目和分辨率,可以提高蛋白质组技术平台的信息完整性。通过对拟南芥双向电泳技术过程中的适当改进,如蛋白质的提取与溶解方法、上样量和聚丙烯酰胺凝胶浓度,加入硫脲,硫代硫酸钠等,对拟南芥双向电泳技术进行了优化,提高了双向电泳图谱的蛋白质点数目与分辨率。 展开更多
关键词 一拟南芥 双向电泳 硫脲 硫代硫酸钠 固相PH梯度
下载PDF
Nitric Oxide Production is Involved in Inhibition Growth of Salicylate at Chilling Temperature
2
作者 夏金婵 何奕昆 《Agricultural Science & Technology》 CAS 2011年第9期1283-1285,共3页
[Objective] The aim was to investigate whether NO is involved in the inhibitory effect of salicylic acid on the growth at low temperature in Arabidopsis.[Method] The endogenous NO level in roots of wild-type and NahG ... [Objective] The aim was to investigate whether NO is involved in the inhibitory effect of salicylic acid on the growth at low temperature in Arabidopsis.[Method] The endogenous NO level in roots of wild-type and NahG mutant growing at 22 or 4 ℃ was measured with NO-specific probe DAF-FM DA staining method.[Result] NO level in plants growing at 4 ℃ for a long term could be increased with time;low temperature could induce NO production in wild-type and NahG mutant,and the NO level in NahG mutant with low level of salicylic acid was significantly lower than that in wild-type.[Conclusion] NO was involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis at low temperature. 展开更多
关键词 Arabidopsis thaliana Low temperature Salicylic acid Nitric oxide
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部