小反刍兽疫是由小反刍兽疫病毒（Peste des petits ru minants，PPRV）引起的一种山羊、绵羊等小反刍动物发生的一种急性接触性传染性疾病，山羊较绵羊易感，具有高发病率和死亡率的特点。感染羊只临床表现为咳嗽、呼吸异常、口腔内坏...小反刍兽疫是由小反刍兽疫病毒（Peste des petits ru minants，PPRV）引起的一种山羊、绵羊等小反刍动物发生的一种急性接触性传染性疾病，山羊较绵羊易感，具有高发病率和死亡率的特点。感染羊只临床表现为咳嗽、呼吸异常、口腔内坏死性病灶、水样腹泻等症状。蓝舌病是由呼肠孤病毒科环状病毒属蓝舌病病毒（Bluetongue virus，BTv）引起的反刍动物发生的一种虫媒传染病。展开更多
Coat protein gene of 12 isolates of Strawberry vein banding virus(SVBV) was studied by multiple sequence alignment and the primers located in conserved region were designed.The detection protocol for SVBV by reverse t...Coat protein gene of 12 isolates of Strawberry vein banding virus(SVBV) was studied by multiple sequence alignment and the primers located in conserved region were designed.The detection protocol for SVBV by reverse transcriptase polymerase chain reaction(RT-PCR) was developed.The primers of multiplex RT-PCR were selected by primer-primer interactions and the melting temperature.The annealing temperature,the concentration of PCR buffer,the extension temperature,the extension time and the concentration of pri-mers were optimized,respectively.A multiplex RT-PCR assay was made for simultaneous detecting Strawberry mottle virus(SMoV),Strawberry mild yellow edge virus(SMYEV) and SVBV.Both field-grown strawberries and microplants were detected effectively.It was the first report that multiplex RT-PCR was used to assay the efficacy of strawberry viruses elimination.展开更多
文摘Coat protein gene of 12 isolates of Strawberry vein banding virus(SVBV) was studied by multiple sequence alignment and the primers located in conserved region were designed.The detection protocol for SVBV by reverse transcriptase polymerase chain reaction(RT-PCR) was developed.The primers of multiplex RT-PCR were selected by primer-primer interactions and the melting temperature.The annealing temperature,the concentration of PCR buffer,the extension temperature,the extension time and the concentration of pri-mers were optimized,respectively.A multiplex RT-PCR assay was made for simultaneous detecting Strawberry mottle virus(SMoV),Strawberry mild yellow edge virus(SMYEV) and SVBV.Both field-grown strawberries and microplants were detected effectively.It was the first report that multiplex RT-PCR was used to assay the efficacy of strawberry viruses elimination.
