To compare serum levels of nitric oxide (NO) and endothelin- 1 (ET- 1), and urinary concentrations of NO and cyclic guanosine monophosphate (cGMP) between preeclamptic and normotensive pregnant women. Method: Ninety-o...To compare serum levels of nitric oxide (NO) and endothelin- 1 (ET- 1), and urinary concentrations of NO and cyclic guanosine monophosphate (cGMP) between preeclamptic and normotensive pregnant women. Method: Ninety-one preeclamptic (48 mild, 43 severe) and forty healthy normotensive pregnant women above 32 gestational weeks were recruited into study. Chemiluminesence technique was used for measuring plasma and urinary NO levels, and radioimmunoassay was used to determine plasma ET- 1 and urinary cGMP levels. Result: Plasma and urinary NO, and urinary cGMP levels were significantly lower in preeclamptics than in the control group (respectively, p < 0.001, p < 0.001, p < 0.01). Plasma ET- 1 levels were significantly higher in the preeclamptics than in the control group (p < 0.001). There were significant negative correlations between plasmaET- 1, plasma NO and urinary NO and cGMP in all groups. There were positive correlations between plasma NO, urinary NO and cGMP in all groups. Conclusion: The imbalance between NO and ET- 1 may play a significant role in the pathophysiology of preeclampsia.展开更多
目的 :了解 n NOS m RNA和 i NOSm RNA在局灶性脑梗死表达中的时程变化与细胞定位。方法 :鼠局灶性脑缺血 /再灌注模型 ;用原位杂交技术进行缺血 /再灌注后 1,3,5天 n NOS m RNA、i NOSm RNA表达的细胞定位并用点杂交方法测量不同时间...目的 :了解 n NOS m RNA和 i NOSm RNA在局灶性脑梗死表达中的时程变化与细胞定位。方法 :鼠局灶性脑缺血 /再灌注模型 ;用原位杂交技术进行缺血 /再灌注后 1,3,5天 n NOS m RNA、i NOSm RNA表达的细胞定位并用点杂交方法测量不同时间段梗死半球与非梗死半球内 n NOS m RNA、i NOSm RNA水平。结果 :正常鼠 n NOSm RNA以神经元表达为主 ,i NOSm RNA无表达 ,梗死后 i NOSm RNA表达以小胶质细胞为主 ,梗死区未见 n NOSm RNA;n NOSm RNA表达从 4h就开始下降 ,1～ 3天最低 ,5天左右又上升 ,i NOSm RNA在缺血再灌注后 12 h开始表达 ,1天左右升至高峰 ,3天左右缓慢下降。结论 :n NOSm RNA在缺血损伤后有一短暂表达增高 ,而在缺血损伤 4h后就开始缓慢下降 ;而 i NOSm RNA在缺血 /再灌注后 12 h开始表达 ,高峰在 1天左右 ,3天左右开始下降 ,其细胞定位以小胶质细胞为主。展开更多
文摘目的:探讨MAdCAM-1在恶唑酮结肠炎中的表达及一氧化氮供体DETA NONOate和GTN对恶唑酮结肠炎的干预作用.方法:32只C57BL/6小鼠随机分为正常对照组(A)、恶唑酮结肠炎组(B)、GTN干预组(C)和DETA NONOate干预组(D).除正常对照组外,其余3组均建立小鼠恶唑酮结肠炎模型,两个一氧化氮供体干预后处死小鼠.评价DAI、大体评分和组织学评分;免疫组织化学法检测MAdCAM-1及NF-КB;硝酸还原酶法测定血清中NO含量;测定结肠组织MPO活性.结果:B组小鼠DAI评分自灌肠之日起逐渐增高,而D组则变化不明显.B组小鼠大体评分、组织学评分、NF-КB的表达、MAdCAM-1的表达、MPO活性(3.13±0.84,20.31±2.63,30.29±8.68,17.60±6.53,3.83±0.60)均分别高于A组(0.38±0.52,0.88±0.83,7.38±2.29,4.08±1.30,1.75±0.25,P<0.01)和D组(1.38±0.52,11.13±1.48,12.60±3.54,8.42±2.16,2.76±0.48,P<0.01),而C组则与B组相近.B组小鼠血清NO浓度高于A组(54.51±22.28 vs 32.17±14.88,P<0.05),而低于D组和C组(54.51±22.28 vs 88.53±24.77,80.12±19.79,均P<0.05).B组MAdCAM-1的表达与组织学评分、MPO活性、NF-КB P65 L4值呈正相关(r=0.786,r=0.833,r=0.833,P<0.05).结论:小鼠恶唑酮结肠炎中结肠固有层MAdCAM-1的表达增加,DETA NONOate可能是治疗溃疡性结肠炎的有价值药物.
文摘To compare serum levels of nitric oxide (NO) and endothelin- 1 (ET- 1), and urinary concentrations of NO and cyclic guanosine monophosphate (cGMP) between preeclamptic and normotensive pregnant women. Method: Ninety-one preeclamptic (48 mild, 43 severe) and forty healthy normotensive pregnant women above 32 gestational weeks were recruited into study. Chemiluminesence technique was used for measuring plasma and urinary NO levels, and radioimmunoassay was used to determine plasma ET- 1 and urinary cGMP levels. Result: Plasma and urinary NO, and urinary cGMP levels were significantly lower in preeclamptics than in the control group (respectively, p < 0.001, p < 0.001, p < 0.01). Plasma ET- 1 levels were significantly higher in the preeclamptics than in the control group (p < 0.001). There were significant negative correlations between plasmaET- 1, plasma NO and urinary NO and cGMP in all groups. There were positive correlations between plasma NO, urinary NO and cGMP in all groups. Conclusion: The imbalance between NO and ET- 1 may play a significant role in the pathophysiology of preeclampsia.
文摘目的 :了解 n NOS m RNA和 i NOSm RNA在局灶性脑梗死表达中的时程变化与细胞定位。方法 :鼠局灶性脑缺血 /再灌注模型 ;用原位杂交技术进行缺血 /再灌注后 1,3,5天 n NOS m RNA、i NOSm RNA表达的细胞定位并用点杂交方法测量不同时间段梗死半球与非梗死半球内 n NOS m RNA、i NOSm RNA水平。结果 :正常鼠 n NOSm RNA以神经元表达为主 ,i NOSm RNA无表达 ,梗死后 i NOSm RNA表达以小胶质细胞为主 ,梗死区未见 n NOSm RNA;n NOSm RNA表达从 4h就开始下降 ,1～ 3天最低 ,5天左右又上升 ,i NOSm RNA在缺血再灌注后 12 h开始表达 ,1天左右升至高峰 ,3天左右缓慢下降。结论 :n NOSm RNA在缺血损伤后有一短暂表达增高 ,而在缺血损伤 4h后就开始缓慢下降 ;而 i NOSm RNA在缺血 /再灌注后 12 h开始表达 ,高峰在 1天左右 ,3天左右开始下降 ,其细胞定位以小胶质细胞为主。
