目的研究内蒙古西部临河地区异常血红蛋白变异类型和异常血红蛋白基因频率。方法运用Hardy-Weinberg平衡法则计算异常血红蛋白在临河地区的基因频率。同时,应用异常血红蛋白化学结构分析方法对内蒙古临河地区发现的5例异常血红蛋白进行...目的研究内蒙古西部临河地区异常血红蛋白变异类型和异常血红蛋白基因频率。方法运用Hardy-Weinberg平衡法则计算异常血红蛋白在临河地区的基因频率。同时,应用异常血红蛋白化学结构分析方法对内蒙古临河地区发现的5例异常血红蛋白进行了一级结构分析。结果对5例异常血红蛋白一级结构分析,证明其分别为Hb G Taipei [β229(B4)Glu-Gly]2例;Hb G Coushatta[β22(B4)Glu-Ala]1例;Hb D Los Angeles[β121(GH4)Glu-Gln]1例和Hb Legnano [α141(HC3)Arg-Leu]1例。临河地区异常血红蛋白的基因频率为:α链异常5.77×10^(-5),β链异常1.15×10^(-4)。结论内蒙古临河地区共发现5种异常血红蛋白,其中Hb Legnano在我国为首次发现。展开更多
Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) is a rather new ’soft ionization’ techniques. Because of its high sensitivity and accuracy, it has been widely used in dete...Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) is a rather new ’soft ionization’ techniques. Because of its high sensitivity and accuracy, it has been widely used in detection and characterization of macromolecules such as peptides, proteins and olignucleotides. It also has been applied successfully in the analysis of posttranslational modification of proteins, such as phosphorylation and glycosylation. Compared with the conventional chemical methods, mass spectrometry is simple, rapid and does not require radiolabeling. In this research, a systematic method to analyse glycoprotein by MALDI-TOF-MS was developed. A practical sample-recombinant human erythropoietin was analysed by the method. The molecular weight, content of carbohydrate and glycosylation sites of the glycoprotein were determined by MALDI-TOF-MS, combined with the endo-glycosidase digestion and peptide mapping. The experimental result shows that MALDI-TOF-MS is a very powerful technique in the characterization of glycosylation proteins.展开更多
文摘目的研究内蒙古西部临河地区异常血红蛋白变异类型和异常血红蛋白基因频率。方法运用Hardy-Weinberg平衡法则计算异常血红蛋白在临河地区的基因频率。同时,应用异常血红蛋白化学结构分析方法对内蒙古临河地区发现的5例异常血红蛋白进行了一级结构分析。结果对5例异常血红蛋白一级结构分析,证明其分别为Hb G Taipei [β229(B4)Glu-Gly]2例;Hb G Coushatta[β22(B4)Glu-Ala]1例;Hb D Los Angeles[β121(GH4)Glu-Gln]1例和Hb Legnano [α141(HC3)Arg-Leu]1例。临河地区异常血红蛋白的基因频率为:α链异常5.77×10^(-5),β链异常1.15×10^(-4)。结论内蒙古临河地区共发现5种异常血红蛋白,其中Hb Legnano在我国为首次发现。
文摘Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) is a rather new ’soft ionization’ techniques. Because of its high sensitivity and accuracy, it has been widely used in detection and characterization of macromolecules such as peptides, proteins and olignucleotides. It also has been applied successfully in the analysis of posttranslational modification of proteins, such as phosphorylation and glycosylation. Compared with the conventional chemical methods, mass spectrometry is simple, rapid and does not require radiolabeling. In this research, a systematic method to analyse glycoprotein by MALDI-TOF-MS was developed. A practical sample-recombinant human erythropoietin was analysed by the method. The molecular weight, content of carbohydrate and glycosylation sites of the glycoprotein were determined by MALDI-TOF-MS, combined with the endo-glycosidase digestion and peptide mapping. The experimental result shows that MALDI-TOF-MS is a very powerful technique in the characterization of glycosylation proteins.
