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狗脑抗吗啡镇痛肽“60a”的分离纯化及部分一级结构
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作者 廖助荣 张申奴 +2 位作者 王镜岩 陈玉珍 周舒 《北京大学学报(自然科学版)》 CAS CSCD 北大核心 1993年第6期680-686,共7页
本实验室从正常狗脑中又分离获得一个暂称之为“60a”的肽。冻干粉经SephadexG-50和羧甲基纤维素CM_(22)柱层析,再经反相高效液相层析纯化得最后样品,经SDS电泳和等电聚焦电泳鉴定均为一条带,分子量约8000,等电点在pH7.8左右;N-末端氨... 本实验室从正常狗脑中又分离获得一个暂称之为“60a”的肽。冻干粉经SephadexG-50和羧甲基纤维素CM_(22)柱层析,再经反相高效液相层析纯化得最后样品,经SDS电泳和等电聚焦电泳鉴定均为一条带,分子量约8000,等电点在pH7.8左右;N-末端氨基酸为Met;自N-末端的部分氨基酸序列为:H_2N-Met-Leu-Ser-Pro-Ala-Asp-Leu-Thr-Asp-Ile-Leu-Phe-Thr-His-Asp-Leu-Ile-Gly-Gly-His-Phe-Arg-Pro-Ala-Lys-Tyr-Lys-Lys-His-Pro-Val-His-Trp-Phe-Val-Ile-Trp-Trp-Pro-……。 展开更多
关键词 分离 抗吗啡作用 一经结构
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Urban and Rural Spatial Layout based on Taiji Interactive Balance of I Ching
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作者 张国勇 陈晓刚 《Agricultural Science & Technology》 CAS 2017年第4期707-709,718,共4页
Taiji is a traditional Chinese philosophy. The content is so diversified that can be applied to so many aspects. The research applied yin and yang, unity be- tween heaven and man to urban planning, and analyzed relati... Taiji is a traditional Chinese philosophy. The content is so diversified that can be applied to so many aspects. The research applied yin and yang, unity be- tween heaven and man to urban planning, and analyzed relationship between urban- rural spatial structure, from transport and material/energy exchange. With the prob- lems reviewed, measures are proposed to deal with the relationship between urban- rural areas in urbanization process. 展开更多
关键词 Taiji of I Ching Spatial structure Urban-rural integration
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