目的:建立七味诃子散中丁香酚含量的HPLC测定方法。方法:采用Phenomenex Luna 5μC18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(55∶45),流速为1 m L·min-1,检测波长为柱温为25℃。结果:丁香酚的进样量在0.564~2.820μg范...目的:建立七味诃子散中丁香酚含量的HPLC测定方法。方法:采用Phenomenex Luna 5μC18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(55∶45),流速为1 m L·min-1,检测波长为柱温为25℃。结果:丁香酚的进样量在0.564~2.820μg范围内线性关系良好(r=0.9999),平均回收率为99.03%(RSD=1.01%);样品中丁香酚的含量测定结果为14.7~15.6 mg·g-1。结论:本方法可用于测定七味诃子散中丁香酚的含量,可为该药质量标准修定提供参考。展开更多
Nine compounds were isolated from the extract of the roots of Oplopanax elatus Nakai(Echinopanax elatus Nakai),family Araliaceae.The chemical structures of six compounds wereestablished by measuring chemical and physi...Nine compounds were isolated from the extract of the roots of Oplopanax elatus Nakai(Echinopanax elatus Nakai),family Araliaceae.The chemical structures of six compounds wereestablished by measuring chemical and physical constants,spectral data(IR,EI-MS,FD-MS,~1HNMR and ^(13)CNMR)and chemical methods,as 1-heptacosanol,β-sitosterol,daucosterol,展开更多
文摘目的:建立七味诃子散中丁香酚含量的HPLC测定方法。方法:采用Phenomenex Luna 5μC18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(55∶45),流速为1 m L·min-1,检测波长为柱温为25℃。结果:丁香酚的进样量在0.564~2.820μg范围内线性关系良好(r=0.9999),平均回收率为99.03%(RSD=1.01%);样品中丁香酚的含量测定结果为14.7~15.6 mg·g-1。结论:本方法可用于测定七味诃子散中丁香酚的含量,可为该药质量标准修定提供参考。
文摘Nine compounds were isolated from the extract of the roots of Oplopanax elatus Nakai(Echinopanax elatus Nakai),family Araliaceae.The chemical structures of six compounds wereestablished by measuring chemical and physical constants,spectral data(IR,EI-MS,FD-MS,~1HNMR and ^(13)CNMR)and chemical methods,as 1-heptacosanol,β-sitosterol,daucosterol,
