Experiments were conducted in a U-shaped open-channel flume with the intention of investigating the bursting phenomena in the meander channel. The experimental results of the secondary flow fields and the Reynolds she...Experiments were conducted in a U-shaped open-channel flume with the intention of investigating the bursting phenomena in the meander channel. The experimental results of the secondary flow fields and the Reynolds shear stress distributions show that the velocity and velocity fluctuation in the transverse direction are not negligible. Moreover, the bursting process is investigated using the three-dimensional quadrant analysis, which is more accurate than using the traditional two-dimensional quadrant analysis for the meandering channel. It is obtained from the experimental results that the internal group of events occurs more frequently than the external group, particularly the internal ejection and internal sweep events. In addition, the transition probabilities of the movements, which are defined as the changes of events from the current situation to the next situation in a time series, show that the stable organizations of events are the most possible movements, whereas the cross organizations of events have the least possible movements.展开更多
In this study, Computational Fluid Dynamics(CFD) is used to investigate and compare the impact of bioreactor parameters(such as its geometry, medium flow-rate, scaffold configuration) on the local transport phenomena ...In this study, Computational Fluid Dynamics(CFD) is used to investigate and compare the impact of bioreactor parameters(such as its geometry, medium flow-rate, scaffold configuration) on the local transport phenomena and, hence, their impact on human mesenchymal stem cell(hM SC) expansion. The geometric characteristics of the TissueFlex174;(Zyoxel Limited, Oxford, UK) microbioreactor were considered to set up a virtual bioreactor containing alginate(in both slab and bead configuration) scaffolds. The bioreactor and scaffolds were seeded with cells that were modelled as glucose consuming entities. The widely used glucose medium, Dulbecco's Modified Eagle Medium(DMEM), supplied at two inlet flow rates of 25 and 100 μl·h^(-1), was modelled as the fluid phase inside the bioreactors. The investigation, based on applying dimensional analysis to this problem, as well as on detailed three-dimensional transient CFD results, revealed that the default bioreactor design and boundary conditions led to internal and external glucose transport, as well as shear stresses, that are conducive to h MSC growth and expansion. Furthermore, results indicated that the ‘top-inout' design(as opposed to its symmetric counterpart) led to higher shear stress for the same media inlet rate(25 μl·h^(-1)), a feature that can be easily exploited to induce shear-dependent differentiation. These findings further confirm the suitability of CFD as a robust design tool.展开更多
To disclose the grain crushing effects on the weathered granular soil rheological behavior,a series of rheological tests (odometer compression and triaxial shearing) were carried out.At the same time,the sieving analy...To disclose the grain crushing effects on the weathered granular soil rheological behavior,a series of rheological tests (odometer compression and triaxial shearing) were carried out.At the same time,the sieving analysis tests of these specimens were also executed before and after tests,and the grain crushing degree,Br and n5,were collectively adopted to estimate the grain crushing.The grain crushing degree depends on the stress path,stress level,and load time,especially,the longer load time and more intensive gradient shearing path will increase the grain crushing quantity.The Hardin crushing degrees Br are 0.191,0.118 and 0.085 in the ordinary compression,rheological compression and triaxial rheological shearing,respectively;The grain crushing degrees n5 are 1.9,1.4 and 1.32,respectively.The strain softening phase indicates the grain crushing and diffusive collapse,and the strain hardening phase indicates the rearrangement of these crushed grains and formation of new bearing soil skeleton.The rheological deformation of granular soil can be attributed to the coarse grain crushing and the filling external porosity with crushed fragments.展开更多
基金Supported by National Natural Science Foundation of China(No.50979066 and No.51279124)Foundation for Creative Research Groups of National Natural Science Foundation of China(No.51021004)
文摘Experiments were conducted in a U-shaped open-channel flume with the intention of investigating the bursting phenomena in the meander channel. The experimental results of the secondary flow fields and the Reynolds shear stress distributions show that the velocity and velocity fluctuation in the transverse direction are not negligible. Moreover, the bursting process is investigated using the three-dimensional quadrant analysis, which is more accurate than using the traditional two-dimensional quadrant analysis for the meandering channel. It is obtained from the experimental results that the internal group of events occurs more frequently than the external group, particularly the internal ejection and internal sweep events. In addition, the transition probabilities of the movements, which are defined as the changes of events from the current situation to the next situation in a time series, show that the stable organizations of events are the most possible movements, whereas the cross organizations of events have the least possible movements.
基金Department of Engineering Science, University of Oxford, Scholarship
文摘In this study, Computational Fluid Dynamics(CFD) is used to investigate and compare the impact of bioreactor parameters(such as its geometry, medium flow-rate, scaffold configuration) on the local transport phenomena and, hence, their impact on human mesenchymal stem cell(hM SC) expansion. The geometric characteristics of the TissueFlex174;(Zyoxel Limited, Oxford, UK) microbioreactor were considered to set up a virtual bioreactor containing alginate(in both slab and bead configuration) scaffolds. The bioreactor and scaffolds were seeded with cells that were modelled as glucose consuming entities. The widely used glucose medium, Dulbecco's Modified Eagle Medium(DMEM), supplied at two inlet flow rates of 25 and 100 μl·h^(-1), was modelled as the fluid phase inside the bioreactors. The investigation, based on applying dimensional analysis to this problem, as well as on detailed three-dimensional transient CFD results, revealed that the default bioreactor design and boundary conditions led to internal and external glucose transport, as well as shear stresses, that are conducive to h MSC growth and expansion. Furthermore, results indicated that the ‘top-inout' design(as opposed to its symmetric counterpart) led to higher shear stress for the same media inlet rate(25 μl·h^(-1)), a feature that can be easily exploited to induce shear-dependent differentiation. These findings further confirm the suitability of CFD as a robust design tool.
基金Project(50908233) supported by the National Natural Science Foundation of ChinaProject(200413) supported by Communication Science and Technology Fund of Hunan Province,China
文摘To disclose the grain crushing effects on the weathered granular soil rheological behavior,a series of rheological tests (odometer compression and triaxial shearing) were carried out.At the same time,the sieving analysis tests of these specimens were also executed before and after tests,and the grain crushing degree,Br and n5,were collectively adopted to estimate the grain crushing.The grain crushing degree depends on the stress path,stress level,and load time,especially,the longer load time and more intensive gradient shearing path will increase the grain crushing quantity.The Hardin crushing degrees Br are 0.191,0.118 and 0.085 in the ordinary compression,rheological compression and triaxial rheological shearing,respectively;The grain crushing degrees n5 are 1.9,1.4 and 1.32,respectively.The strain softening phase indicates the grain crushing and diffusive collapse,and the strain hardening phase indicates the rearrangement of these crushed grains and formation of new bearing soil skeleton.The rheological deformation of granular soil can be attributed to the coarse grain crushing and the filling external porosity with crushed fragments.
