目的:异常咬合信息可以通过牙周本体感受器、三叉神经系向高级中枢传递,三叉神经感觉主核背内侧部(dorsomedial part of the trigeminal nucleus,Vpdm)-丘脑腹后内侧核(ventral posteromedial thalamic nucleus,VPM)是重要的三叉神经系...目的:异常咬合信息可以通过牙周本体感受器、三叉神经系向高级中枢传递,三叉神经感觉主核背内侧部(dorsomedial part of the trigeminal nucleus,Vpdm)-丘脑腹后内侧核(ventral posteromedial thalamic nucleus,VPM)是重要的三叉神经系上传通路,Vpdm是否会受到异常咬合影响,是否继续上传至VPM值得深入研究。本文通过检测单侧前牙反[牙合](unilateral anterior crossbite,UAC)大鼠,Vpdm及VPM中兴奋性神经传导标志物—囊泡膜谷氨酸转运体1,2(vesicular glutamate transporter1,2,VGLUT1,2)的表达变化,探讨该问题。方法:将雌性大鼠42只分为UAC组和对照组,造模后2、4和8周取材,以免疫荧光、Western blot及实时定量PCR技术,检测目标神经核团中VGLUT1,2的表达差异。结果:UAC组Vpdm内VGLUT1蛋白和mRNA的表达在各个时间点均明显高于对照组,而VGLUT2 mRNA的表达则无显著性差异。在VPM内,VGLUT1,2的蛋白水平和mRNA水平两组间均无显著差异。结论:UAC促进了Vpdm的VGLUT1表达,但对VPM的VGLUT1,2表达水平没有显著影响。展开更多
To discuss the hazard of migraine in acute phase and analgesic effect, acupoint selection features, method as well as mechanism of acupuncture; to put forward relevant questions, answer these questions, propose relati...To discuss the hazard of migraine in acute phase and analgesic effect, acupoint selection features, method as well as mechanism of acupuncture; to put forward relevant questions, answer these questions, propose relative specificity hypothesis on neural pathway of analgesic effect of acupoints in different nerve distribution areas（trigeminal nerve distribution area, occipital nerve distribution area, and spinal nerve distribution area） in treatment of migraine in acute phase, and analyze the deficiencies of hypothesis.展开更多
文摘目的:异常咬合信息可以通过牙周本体感受器、三叉神经系向高级中枢传递,三叉神经感觉主核背内侧部(dorsomedial part of the trigeminal nucleus,Vpdm)-丘脑腹后内侧核(ventral posteromedial thalamic nucleus,VPM)是重要的三叉神经系上传通路,Vpdm是否会受到异常咬合影响,是否继续上传至VPM值得深入研究。本文通过检测单侧前牙反[牙合](unilateral anterior crossbite,UAC)大鼠,Vpdm及VPM中兴奋性神经传导标志物—囊泡膜谷氨酸转运体1,2(vesicular glutamate transporter1,2,VGLUT1,2)的表达变化,探讨该问题。方法:将雌性大鼠42只分为UAC组和对照组,造模后2、4和8周取材,以免疫荧光、Western blot及实时定量PCR技术,检测目标神经核团中VGLUT1,2的表达差异。结果:UAC组Vpdm内VGLUT1蛋白和mRNA的表达在各个时间点均明显高于对照组,而VGLUT2 mRNA的表达则无显著性差异。在VPM内,VGLUT1,2的蛋白水平和mRNA水平两组间均无显著差异。结论:UAC促进了Vpdm的VGLUT1表达,但对VPM的VGLUT1,2表达水平没有显著影响。
文摘To discuss the hazard of migraine in acute phase and analgesic effect, acupoint selection features, method as well as mechanism of acupuncture; to put forward relevant questions, answer these questions, propose relative specificity hypothesis on neural pathway of analgesic effect of acupoints in different nerve distribution areas（trigeminal nerve distribution area, occipital nerve distribution area, and spinal nerve distribution area） in treatment of migraine in acute phase, and analyze the deficiencies of hypothesis.
