Dibromocephalomannine(DIBROCs)is the intermediate product in the purification of taxol. In this paper, we successfully realized the efficient conversion of DIBROCs to cephalomannine under ultrasound irradiation for th...Dibromocephalomannine(DIBROCs)is the intermediate product in the purification of taxol. In this paper, we successfully realized the efficient conversion of DIBROCs to cephalomannine under ultrasound irradiation for the first time. Ultrasound irradiation, a favorable acceleration of reaction rates, could solve the problems of incomplete conversion of DIBROCs to cephalomannine and the by-products due to long reaction time under the traditional stirring condition. The results showed that under ultrasound irradiation zinc powder was more effective than other metal powder and the proper reaction temperature was 20 ℃. Under this condition the conversion was more than 98% in 40 min.展开更多
将自制的红豆杉果酒注入质谱仪,确定红豆杉在白酒中的特征成分为紫杉醇和三尖杉宁碱。红豆杉酒样品用70%(体积分数)甲醇溶液稀释,离心后,采用高效液相色谱-串联质谱法测定上清液中紫杉醇和三尖杉宁碱的含量。以ACQUITY UPLC R BEH C18...将自制的红豆杉果酒注入质谱仪,确定红豆杉在白酒中的特征成分为紫杉醇和三尖杉宁碱。红豆杉酒样品用70%(体积分数)甲醇溶液稀释,离心后,采用高效液相色谱-串联质谱法测定上清液中紫杉醇和三尖杉宁碱的含量。以ACQUITY UPLC R BEH C18色谱柱(2.1 mm×50mm,1.7μm)为固定相,以不同体积比的甲醇和水的混合液为流动相进行梯度洗脱,串联质谱分析中采用电喷雾离子源正离子模式和多反应监测模式。紫杉醇和三尖杉宁碱的质量浓度在一定范围内与其对应的峰面积呈线性关系,检出限(3S/N)均为2.5μg·kg-1,测定下限(10S/N)均为8.3μg·kg-1。以空白样品为基体进行加标回收试验,所得回收率为96.4%~116%,测定值的相对标准偏差(n=6)为1.1%~2.6%。展开更多
目的:测定并比较维吾尔药材喜马拉雅红豆杉与人工栽培品种曼地亚红豆杉中紫杉醇和三尖杉宁碱的含量。方法采用RP‐HPLC色谱法梯度洗脱,色谱柱为 Century SIL C18‐EPS 柱(250 mm x4.6 mm ,5μm );流动相为乙腈‐水;流速为1.0 m...目的:测定并比较维吾尔药材喜马拉雅红豆杉与人工栽培品种曼地亚红豆杉中紫杉醇和三尖杉宁碱的含量。方法采用RP‐HPLC色谱法梯度洗脱,色谱柱为 Century SIL C18‐EPS 柱(250 mm x4.6 mm ,5μm );流动相为乙腈‐水;流速为1.0 mL· min-1;检测波长为227 nm ;柱温为30℃。结果紫杉醇回归方程:Y=2.050975x107 X+5531.887,线性范围为0.042~1.69μg(r=0.9999),平均回收率为98.0%,RSD=0.98%(n=6);三尖杉宁碱回归方程Y=1.806535x107 X+3046.378,线性范围为0.040~1.6μg(r=0.9999),平均回收率为96.6%,RSD=2.97%(n=6),喜马拉雅红豆杉叶中三尖杉宁碱平均含量高于茎中含量,曼地亚红豆杉相反;两者叶中紫杉醇含量高于茎中含量,而曼地亚红豆杉茎叶中紫杉醇含量并不低于喜马拉雅红豆杉。结论该方法简便、准确、适用于红豆杉药材的质量评价。展开更多
文摘Dibromocephalomannine(DIBROCs)is the intermediate product in the purification of taxol. In this paper, we successfully realized the efficient conversion of DIBROCs to cephalomannine under ultrasound irradiation for the first time. Ultrasound irradiation, a favorable acceleration of reaction rates, could solve the problems of incomplete conversion of DIBROCs to cephalomannine and the by-products due to long reaction time under the traditional stirring condition. The results showed that under ultrasound irradiation zinc powder was more effective than other metal powder and the proper reaction temperature was 20 ℃. Under this condition the conversion was more than 98% in 40 min.
文摘目的:测定并比较维吾尔药材喜马拉雅红豆杉与人工栽培品种曼地亚红豆杉中紫杉醇和三尖杉宁碱的含量。方法采用RP‐HPLC色谱法梯度洗脱,色谱柱为 Century SIL C18‐EPS 柱(250 mm x4.6 mm ,5μm );流动相为乙腈‐水;流速为1.0 mL· min-1;检测波长为227 nm ;柱温为30℃。结果紫杉醇回归方程:Y=2.050975x107 X+5531.887,线性范围为0.042~1.69μg(r=0.9999),平均回收率为98.0%,RSD=0.98%(n=6);三尖杉宁碱回归方程Y=1.806535x107 X+3046.378,线性范围为0.040~1.6μg(r=0.9999),平均回收率为96.6%,RSD=2.97%(n=6),喜马拉雅红豆杉叶中三尖杉宁碱平均含量高于茎中含量,曼地亚红豆杉相反;两者叶中紫杉醇含量高于茎中含量,而曼地亚红豆杉茎叶中紫杉醇含量并不低于喜马拉雅红豆杉。结论该方法简便、准确、适用于红豆杉药材的质量评价。