A simple and rapid colorimetric method for the and Whatman filter paper chromatography is described. determination of some amino acids on thin layer chromatography （TLC） The studied compounds are cysteine, proline, ...A simple and rapid colorimetric method for the and Whatman filter paper chromatography is described. determination of some amino acids on thin layer chromatography （TLC） The studied compounds are cysteine, proline, methionine, tryptophan glutmine, glycine, thyrosine, threonine, leucine and lysine. The method is based on the formation of color from the reaction of stannous chloride （SnCI2） and ninhydrin reagents. The procedure has been successfully applied to the assay of the standard amino acid preparation after TLC separation, and the results of the studied compounds were clear and favorable, comparing to the official methods. However, the improved TLC is much more efficient for the detection of amino acid. The main advantage of this method is that the detection of amino acids can be preformed visually.展开更多
Horseshoe vortex topological structure has been studied extensively in the past,traditional"saddle of separation"and new"attachment saddle point"topologies found in literature both have theoretical...Horseshoe vortex topological structure has been studied extensively in the past,traditional"saddle of separation"and new"attachment saddle point"topologies found in literature both have theoretical basis and experimental and computational evidences for support.The laminar incompressible juncture flows at low Reynolds numbers especially are observed to have new topology.Studies concerning the existence of the new topology though found in literature,the topological evolution and its dependency on various critical flow parameters require further investigation.A Particle Image Velocimetry based analysis is carried out to observe the effect of aspect ratio,?*/D and shape of the obstacle on laminar horseshoe vortex topology for small obstacles.Rise in aspect ratio evolves the topology from the traditional to new for all the cases observed.The circular cross section obstacles are found more apt to having the new topology compared to square cross sections.It is noted that the sweeping effect of the fluid above the vortex system in which horseshoe vortex is immersed plays a critical role in this evolution.Topological evolution is observed not only in the most upstream singular point region of horseshoe vortex system but also in the corner region.The corner vortex topology evolves from the traditional type to new one before the topological evolution of the most upstream singular point,resulting in a new topological pattern of the laminar juncture flows"separation-attachment combination".The study may help extend the understanding of the three-dimensional boundary layer separation phenomenon.展开更多
文摘A simple and rapid colorimetric method for the and Whatman filter paper chromatography is described. determination of some amino acids on thin layer chromatography （TLC） The studied compounds are cysteine, proline, methionine, tryptophan glutmine, glycine, thyrosine, threonine, leucine and lysine. The method is based on the formation of color from the reaction of stannous chloride （SnCI2） and ninhydrin reagents. The procedure has been successfully applied to the assay of the standard amino acid preparation after TLC separation, and the results of the studied compounds were clear and favorable, comparing to the official methods. However, the improved TLC is much more efficient for the detection of amino acid. The main advantage of this method is that the detection of amino acids can be preformed visually.
基金supported by the National Natural Science Foundation of China(Grant No.11372027)
文摘Horseshoe vortex topological structure has been studied extensively in the past,traditional"saddle of separation"and new"attachment saddle point"topologies found in literature both have theoretical basis and experimental and computational evidences for support.The laminar incompressible juncture flows at low Reynolds numbers especially are observed to have new topology.Studies concerning the existence of the new topology though found in literature,the topological evolution and its dependency on various critical flow parameters require further investigation.A Particle Image Velocimetry based analysis is carried out to observe the effect of aspect ratio,?*/D and shape of the obstacle on laminar horseshoe vortex topology for small obstacles.Rise in aspect ratio evolves the topology from the traditional to new for all the cases observed.The circular cross section obstacles are found more apt to having the new topology compared to square cross sections.It is noted that the sweeping effect of the fluid above the vortex system in which horseshoe vortex is immersed plays a critical role in this evolution.Topological evolution is observed not only in the most upstream singular point region of horseshoe vortex system but also in the corner region.The corner vortex topology evolves from the traditional type to new one before the topological evolution of the most upstream singular point,resulting in a new topological pattern of the laminar juncture flows"separation-attachment combination".The study may help extend the understanding of the three-dimensional boundary layer separation phenomenon.
