Acute promyelocytic leukemia (APL) is a malignant and fatal disease. The authors report here for the first time in the world that a chemically synthesized diarsenic trisulfide (DATS, As 2S 3) used alone by mouth can h...Acute promyelocytic leukemia (APL) is a malignant and fatal disease. The authors report here for the first time in the world that a chemically synthesized diarsenic trisulfide (DATS, As 2S 3) used alone by mouth can have a remarkable effect in the treatment. The patient is reported to have attained not only hematological/clinical complete remission, but also cytogenetic and molecular biological remission after treatment. A male 47 year old patient with APL which was not treated before was given DATS by mouth from Nov.18, 1998. The dosage was 0.5 g tid. Promyelocytes in his bone marrow were decreased from 90% to normal range. Alongside, there was normalization of blood platelet count and hemoglobin value. He was discharged with hematological remission on Dec.31, 1998, and attained cytogenetic and molecular biological remission in March 1999. The side effect was minimal.展开更多
William Tongamp,et al.研究了在Cu3AsS4-NaHS-NaOH体系中从高碱性硫砷铜矿浸出液中脱砷的可行性和浸出剂的再生性。首先还原硫元素然后将亚砷酸盐（As（Ⅲ））氧化成砷酸盐（As（Ⅴ））以形成沉淀Na3AsS4。首先,在NaHS-NaOH体系中,用...William Tongamp,et al.研究了在Cu3AsS4-NaHS-NaOH体系中从高碱性硫砷铜矿浸出液中脱砷的可行性和浸出剂的再生性。首先还原硫元素然后将亚砷酸盐（As（Ⅲ））氧化成砷酸盐（As（Ⅴ））以形成沉淀Na3AsS4。首先,在NaHS-NaOH体系中,用三硫化二砷（As2S3）或硫砷铜矿（Cu3AsS4）制备砷质量浓度35g／L以上的溶液。展开更多
Objective: To evaluate whether realgar could down-regulate human telomerase reverse transcriptase (hTERT) gene expression and telomerase activity in acute promyelocytic leukemia cell line-NB4 cells. Methods: The expre...Objective: To evaluate whether realgar could down-regulate human telomerase reverse transcriptase (hTERT) gene expression and telomerase activity in acute promyelocytic leukemia cell line-NB4 cells. Methods: The expression of hTERT-mRNA was analyzed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay (PCR-ELISA). Flow cytometry using PI staining was applied to analyze the cell cycle and apoptosis. Results: Treatment of NB4 cells with 155, 300, 600 μg/L realgar reduced telomerase activity significantly accompanying with decrease of hTERT-mRNA and increasing cell apoptosis. G2/M phase arrest appeared when treated with realgar in 300, 600 μg/L. Conclusion: It is suggested that telomerase activity of NB4 cells can be specifically inhibited by realgar through the down-regulation of hTERT gene expression. G2/M phase arrest and apoptosis by realgar in NB4 cells might be related to the reduction of telomerase activity and hTERT-mRNA expression.展开更多
文摘Acute promyelocytic leukemia (APL) is a malignant and fatal disease. The authors report here for the first time in the world that a chemically synthesized diarsenic trisulfide (DATS, As 2S 3) used alone by mouth can have a remarkable effect in the treatment. The patient is reported to have attained not only hematological/clinical complete remission, but also cytogenetic and molecular biological remission after treatment. A male 47 year old patient with APL which was not treated before was given DATS by mouth from Nov.18, 1998. The dosage was 0.5 g tid. Promyelocytes in his bone marrow were decreased from 90% to normal range. Alongside, there was normalization of blood platelet count and hemoglobin value. He was discharged with hematological remission on Dec.31, 1998, and attained cytogenetic and molecular biological remission in March 1999. The side effect was minimal.
基金Supported by Xi'an Foundation of Science and Technology Program(200016)
文摘Objective: To evaluate whether realgar could down-regulate human telomerase reverse transcriptase (hTERT) gene expression and telomerase activity in acute promyelocytic leukemia cell line-NB4 cells. Methods: The expression of hTERT-mRNA was analyzed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay (PCR-ELISA). Flow cytometry using PI staining was applied to analyze the cell cycle and apoptosis. Results: Treatment of NB4 cells with 155, 300, 600 μg/L realgar reduced telomerase activity significantly accompanying with decrease of hTERT-mRNA and increasing cell apoptosis. G2/M phase arrest appeared when treated with realgar in 300, 600 μg/L. Conclusion: It is suggested that telomerase activity of NB4 cells can be specifically inhibited by realgar through the down-regulation of hTERT gene expression. G2/M phase arrest and apoptosis by realgar in NB4 cells might be related to the reduction of telomerase activity and hTERT-mRNA expression.
