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小型猪骨膜干细胞的分离与鉴定
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作者 王安鸿 史尉利 +4 位作者 余慧镭 傅欣 段小宁 胡晓青 郭秦炜 《中国运动医学杂志》 CAS CSCD 北大核心 2020年第9期719-724,共6页
目的:研究哺乳动物的骨膜中是否存在间充质干细胞。方法:取1月半龄的贵州小香猪髂骨近端内侧的骨膜,经I型和II型胶原酶消化分离骨膜细胞,在标准条件下体外细胞培养约3周,加入抗CD90,CD44,CD105,CD14,CD45的单克隆抗体孵育后,经流式细胞... 目的:研究哺乳动物的骨膜中是否存在间充质干细胞。方法:取1月半龄的贵州小香猪髂骨近端内侧的骨膜,经I型和II型胶原酶消化分离骨膜细胞,在标准条件下体外细胞培养约3周,加入抗CD90,CD44,CD105,CD14,CD45的单克隆抗体孵育后,经流式细胞学分析;再通过干细胞分化试剂盒诱导成骨、成软骨和成脂分化,鉴定其干细胞特性。结果:从猪骨膜分离消化的细胞,经体外培养3周后,细胞呈梭形或不规则三角形生长,胞质向外伸出长短不同的突起;流式细胞学分析显示从骨膜分离的细胞能表达表面抗原CD44、CD90和CD105,而不表达CD14和CD45;经诱导该细胞能向成骨、成软骨和成脂分化。结论:本研究成功分离并鉴定了小型猪骨膜来源的干细胞(Periosteal derived stem cells,PDSCs),为软骨缺损修复的组织工程技术提供了种子细胞来源。 展开更多
关键词 骨膜 干细胞 分离 鉴定 三系分化
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Ternary compounds and solid-state phase equilibria in Mg-rich side of Mg-Zn-Ca system at 300 ℃
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作者 李洪晓 任玉平 +2 位作者 马倩倩 蒋敏 秦高梧 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2011年第10期2147-2153,共7页
The phase equilibria and compositions in Mg-rich side at 300 ℃ were investigated in Mg-Zn-Ca ternary system through the equilibrated alloy method by using scanning electron microscopy,electron probe microanalysis,X-r... The phase equilibria and compositions in Mg-rich side at 300 ℃ were investigated in Mg-Zn-Ca ternary system through the equilibrated alloy method by using scanning electron microscopy,electron probe microanalysis,X-ray diffraction and transmission electron microscopy.The results show that two ternary compounds T1 and T2 can be in equilibrium with the Mg-based solid solution in Mg-Zn-Ca system.T1 phase is a linear compound with the composition region(molar fraction) of 15% Ca,20.5%-48.9% Zn and balanced Mg at 300 ℃.Its hexagonal structure parameters decrease with increasing Zn content,i.e.a=0.992-0.945 nm,c=1.034-1.003 nm.T2 phase has hexagonal structure with the composition region of 26.4%-28.4% Mg,63.2%-65.5% Zn and 7.1%-8.4% Ca.At 300 ℃,the solubility of Zn in the Mg-based solid solution increases for the addition of Ca,the maximum solubility of Zn is 3.7%.Three-phase fields consisting of--Mg+Mg2Ca+T1,--Mg+T1+T2,--Mg+T2+MgZn and MgZn+T2+Mg2Zn3 exist in the Mg-Zn-Ca system at 300 ℃. 展开更多
关键词 Mg-Zn-Ca system ternary compound STRUCTURE COMPOSITION phase equilibrium
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TAMDAR Observation Assimilation in WRF 3D-Var and Its Impact on Hurricane Ike (2008) Forecast 被引量:2
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作者 Hong-Li WANG Xiang-Yu HUANG 《Atmospheric and Oceanic Science Letters》 2012年第3期206-211,共6页
This study evaluates the impact of atmospheric observations from the Tropospheric Airborne Meteorological Data Reporting (TAMDAR) observing system on numerical weather prediction of hurricane Ike (2008) using three-di... This study evaluates the impact of atmospheric observations from the Tropospheric Airborne Meteorological Data Reporting (TAMDAR) observing system on numerical weather prediction of hurricane Ike (2008) using three-dimensional data assimilation system for the Weather Research and Forecast (WRF) model (WRF 3D-Var). The TAMDAR data assimilation capability is added to WRF 3D-Var by incorporating the TAMDAR observation operator and corresponding observation processing procedure. Two 6-h cycling data assimilation and forecast experiments are conducted. Track and intensity forecasts are verified against the best track data from the National Hurricane Center. The results show that, on average, assimilating TAMDAR observations has a positive impact on the forecasts of hurricane Ike. The TAMDAR data assimilation reduces the track errors by about 30 km for 72-h forecasts. Improvements in intensity forecasts are also seen after four 6-h data assimilation cycles. Diagnostics show that assimilation of TAMDAR data improves subtropical ridge and steering flow in regions along Ike's track, resulting in better forecasts. 展开更多
关键词 data assimilation TAMDAR numerical weather prediction HURRICANE WRF
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