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水蕨血红蛋白基因的分子克隆和序列分析 被引量:1
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作者 齐小琼 葛亚飞 李大卫 《广西植物》 CAS CSCD 北大核心 2016年第2期216-223,161,共9页
植物血红蛋白(Hemoglobin)是一类由珠蛋白(Globin)和血红素(Ferroheme)组成的结合蛋白,在植物中广泛分布,迄今已在苔藓植物、裸子植物和被子植物中克隆到血红蛋白基因序列,但在蕨类植物中相关研究还未见报道。该研究采用热不对称交错PCR... 植物血红蛋白(Hemoglobin)是一类由珠蛋白(Globin)和血红素(Ferroheme)组成的结合蛋白,在植物中广泛分布,迄今已在苔藓植物、裸子植物和被子植物中克隆到血红蛋白基因序列,但在蕨类植物中相关研究还未见报道。该研究采用热不对称交错PCR(TAIL-PCR)方法克隆了水蕨血红蛋白基因的全长序列。该基因的序列总长为949 bp,包含4个外显子和3个内含子,编码189个氨基酸。预测的蛋白质(命名为CtHb)的分子量为21.14 kDa,等电点(pI)为7.81。三维结构模拟表明CtHb具有植物血红蛋白典型的三级结构:即含有A、B、C、E、F、G和H螺旋,形成了3-on-3的"三明治"结构。和水稻血红蛋白的三级结构相比,CtHb的大部分结构(包括具有远端和近端组氨酸定位的E螺旋和F螺旋的位置等)同水稻的结构极为相似。两者的不同之处主要表现在:(1)CtHb含有较长的N-端区域;(2)两者CD-loop的折叠方式不同;(3)两者螺旋B和螺旋C的连接方式不同,CtHb是通过卷曲连接的,而水稻中借助的是螺旋。结构进化分析揭示了植物血红蛋白从非共生到共生进化过程中的一些关键改变,这些改变可能有助于非共生血红蛋白向共生血红蛋白结构的转变,特别是有助于豆血红蛋白共生功能的实现。 展开更多
关键词 植物血红蛋白 序列分析 三级结构模拟 结构进化 水蕨
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Three-dimensional structure of mesoscale eddies in the western tropical Pacific as revealed by a high-resolution ocean simulation 被引量:1
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作者 WANG QingYe 《Science China Earth Sciences》 SCIE EI CAS CSCD 2017年第9期1719-1731,共13页
The three-dimensional structure of mesoscale eddies in the western tropical Pacific(6°S–20°N, 120°E–150°E)is investigated using a high-resolution ocean model simulation. Eddy detection and eddy t... The three-dimensional structure of mesoscale eddies in the western tropical Pacific(6°S–20°N, 120°E–150°E)is investigated using a high-resolution ocean model simulation. Eddy detection and eddy tracking algorithms are applied to simulated horizontal velocity vectors, and the anticyclonic and cyclonic eddies identified are composited to obtain their three-dimensional structures. The mean lifetime of all long-lived eddies is about 52 days, and their mean diameter is 147 km. Two typical characteristics of mesoscale eddies are revealed and possible dynamic explanations are analyzed. One typical characteristic is that surface eddies are generally separated from subthermocline eddies along the bifurcation latitude(~13°N) of the North Equatorial Current in the western tropical Pacific, which may be associated with different eddy energy sources and vertical eddy energy fluxes in subtropical and tropical gyres. Surface eddies have maximum swirl velocities of 8–9 cm s^(-1) and can extend to about 1500 m depth. Subthermocline eddies occur below 200 m, with their cores at about 400–600 m depth, and their maximum swirl velocities can reach 10 cm s^(-1). The other typical characteristic is that the meridional velocity component of the eddy is much larger than the zonal component. This characteristic might be due to more zonal eddy pairs(two eddies at the same latitude),which is also supported by the zonal wavelength(about 200 km) in the high-frequency meridional velocity component of the horizontal velocity. 展开更多
关键词 Ocean eddies Western tropical Pacific Three-dimensional structure
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