Glutathione S-transferases （GSTs） are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis ph...Glutathione S-transferases （GSTs） are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs （VpGSTs） and four Mytilus galloprovincialis GSTs （MgGSTs） following exposure to BDE-47. Differential expressions of the seven VpGSTs and four MgGSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes （VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3） were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking, Hydrophobic and n were the most often observed interactions between BDE-47 and the GSTs.展开更多
Inspired by the potential computational capability of 3-Dimensional (3D) DNA structure,this paper presents a graph structure constructed by k-armed (k = 3or 4) branched junction DNA molecules to explore the possibilit...Inspired by the potential computational capability of 3-Dimensional (3D) DNA structure,this paper presents a graph structure constructed by k-armed (k = 3or 4) branched junction DNA molecules to explore the possibility of solving some intractable problems. In the proposed procedure,vertex building blocks consisting of 3,4-armed branched junction molecules are selectively used to form different graph structures. After separating these graph structures by gel electrophoresis,the connec-tivity of this graph can be determined. Furthermore,the amount of potential solutions can be reduced by a theorem of graph theory.展开更多
基金Supported by the National Natural Science Foundation of China(No.21107136)the International Foundation for Science(No.F/5230-1)
文摘Glutathione S-transferases （GSTs） are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs （VpGSTs） and four Mytilus galloprovincialis GSTs （MgGSTs） following exposure to BDE-47. Differential expressions of the seven VpGSTs and four MgGSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes （VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3） were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking, Hydrophobic and n were the most often observed interactions between BDE-47 and the GSTs.
基金Supported by the National Natural Science Foundation of China (No.30370356 and No.60574041).
文摘Inspired by the potential computational capability of 3-Dimensional (3D) DNA structure,this paper presents a graph structure constructed by k-armed (k = 3or 4) branched junction DNA molecules to explore the possibility of solving some intractable problems. In the proposed procedure,vertex building blocks consisting of 3,4-armed branched junction molecules are selectively used to form different graph structures. After separating these graph structures by gel electrophoresis,the connec-tivity of this graph can be determined. Furthermore,the amount of potential solutions can be reduced by a theorem of graph theory.
