The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days, In this paper, 40 color-different crab individuals we...The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days, In this paper, 40 color-different crab individuals were evaluated using 80 RAPD primers. 30 primers were selected using bulk segregation analysis （BSA） and RAPD-PCR analysis. The results showed that the ratio of polymorphic loci was 61.29%, and Nei＇s gene diversity was 0.375 6, and Shannon＇s information index was 0.554 5 among purple crab individuals; and the ratio of polymorphic loci was 62.44%, and Nei＇s gene diversity was 0.3451, and Shannon＇s information index was 0.5099 among tea-green crab individuals. It was clear that the genetic diversity between purple and tea-green crabs was similar, which indicated close phylogenetic relationships between the purple and tea-green crabs. In addition, two primers （SBS A09 and SBS G16） got specific DNA bands by comparing forty individuals of color-different crab individuals through RAPD-PCR analysis and the following electrophoresis. They indicated close phylogenetic relationships between purple and tea-green crabs.展开更多
基金supported by the grant of the Key Project in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2006BAD09A01)
文摘The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days, In this paper, 40 color-different crab individuals were evaluated using 80 RAPD primers. 30 primers were selected using bulk segregation analysis （BSA） and RAPD-PCR analysis. The results showed that the ratio of polymorphic loci was 61.29%, and Nei＇s gene diversity was 0.375 6, and Shannon＇s information index was 0.554 5 among purple crab individuals; and the ratio of polymorphic loci was 62.44%, and Nei＇s gene diversity was 0.3451, and Shannon＇s information index was 0.5099 among tea-green crab individuals. It was clear that the genetic diversity between purple and tea-green crabs was similar, which indicated close phylogenetic relationships between the purple and tea-green crabs. In addition, two primers （SBS A09 and SBS G16） got specific DNA bands by comparing forty individuals of color-different crab individuals through RAPD-PCR analysis and the following electrophoresis. They indicated close phylogenetic relationships between purple and tea-green crabs.
