Background/Aims CD4+ lymphocytes constitutively expressing the IL-2-receptor α-chain (CD25) regulate the activation of CD4 and CD8 autoreactive T-cells by suppressing their proliferation and effector function. The ai...Background/Aims CD4+ lymphocytes constitutively expressing the IL-2-receptor α-chain (CD25) regulate the activation of CD4 and CD8 autoreactive T-cells by suppressing their proliferation and effector function. The aim of this study is to:(1) measure the percentage of CD4+ CD25+ T-cells (T-regs)in patients with autoimmune liver disease at presentation and during remission, (2) correlate their frequency with disease activity, (3) determine their ability to expand and (4) to inhibit interferon-gamma (IFNγ ) production by CD4+ CD25-T-cells.Methods 41 patients were studied. Percentage of T-regs was determined on peripheral blood mononuclear cells (PBMCs) by triple-colour flow cytometry; their ability to expand by exposing PBMCs to a T-cell expander (CD3/CD28 Dynabeads); their immunoregulatory function by measuring their ability to suppress IFNγ production by CD4+ CD25-T-cells. Results T-regs were significantly less in patients than in controls, and at diagnosis than during remission. Their percentage was inversely correlated with titres of anti-liver kidney microsomal and soluble liver antigen autoantibodies. T-regs ability to expand was significantly lower in patients than in controls, but that to suppress IFNγ production by CD4+ CD25-T-cells was maintained. Conclusions Decreased T-regs numbers and ability to expand may favour the emergence of liver-targeted autoimmunity, despite preserved suppressor function. Treatment should aim at increasing T-regs number.展开更多
文摘Background/Aims CD4+ lymphocytes constitutively expressing the IL-2-receptor α-chain (CD25) regulate the activation of CD4 and CD8 autoreactive T-cells by suppressing their proliferation and effector function. The aim of this study is to:(1) measure the percentage of CD4+ CD25+ T-cells (T-regs)in patients with autoimmune liver disease at presentation and during remission, (2) correlate their frequency with disease activity, (3) determine their ability to expand and (4) to inhibit interferon-gamma (IFNγ ) production by CD4+ CD25-T-cells.Methods 41 patients were studied. Percentage of T-regs was determined on peripheral blood mononuclear cells (PBMCs) by triple-colour flow cytometry; their ability to expand by exposing PBMCs to a T-cell expander (CD3/CD28 Dynabeads); their immunoregulatory function by measuring their ability to suppress IFNγ production by CD4+ CD25-T-cells. Results T-regs were significantly less in patients than in controls, and at diagnosis than during remission. Their percentage was inversely correlated with titres of anti-liver kidney microsomal and soluble liver antigen autoantibodies. T-regs ability to expand was significantly lower in patients than in controls, but that to suppress IFNγ production by CD4+ CD25-T-cells was maintained. Conclusions Decreased T-regs numbers and ability to expand may favour the emergence of liver-targeted autoimmunity, despite preserved suppressor function. Treatment should aim at increasing T-regs number.
文摘目的分析急性髓系细胞白血病 M1(AML M1)免疫学特征及预后,探讨其与 AML M2及急性淋巴细胞白血病(ALL)的免疫学鉴别要点。方法采用 CD45/SSC 双参数散点图设门技术,应用三色流式细胞术,对47例 M1初诊患者骨髓标本进行免疫分型,并对其中17例进行核型分析。并用同期51例 M2初诊患者及58例 ALL
