[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and externa...[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and external morphology. [Method] The paraffin section method was used to observe the flower bud of Gentiana triflora un- der microscope. [Result] The flower bud differentiation process of Gentiana triflora could be divided into six stages： the undifferentiated stage, initial differentiation stage, sepal primordia differentiation stage, petal primordia differentiation stage, stamen pri- mordia differentiation stage and the pistil primordia differentiation stage. The flower bud differentiation started from early May, when the plant had 10-11 stem nodes, and completed in early June, lasted about 40 d. The effect of plant height on flower bud differentiation was not significant. [Conclusion] The study provided theoretical basis for the high-yield cultivation, variety selection and flowering regulation of Gentiana triflora.展开更多
[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of tri...[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of triazophos manufacture. Then the strain C-Y106 was identified according to the morphology,physiological and biochemical characteristics,and 16S rRNA sequence analysis. The effect of medium with different nutrients on triazophos-degrading rate by C-Y106 was studied. [Result] The strain C-Y106 was identified as Bacillus subtilis. The strain C-Y106 could grow in the mineral salt medium with 40 mg/L of triazophos as the sole sources of carbon,Nitrogen and Phosphorus. The triazophos-degrading rate was the highest as 76.8% in the mineral salt medium with 40 mg/L of triazophos as the sole source of Phosphorus,after being incubated at 31 ℃,pH 8.0 and 150 r/min for 60 h. [Conclusion] The research had provided theoretical basis for the identification and purification of enzymes for triazophos degradation.展开更多
To optimize the regeneration system of Bougainvillea, the effects of different hormone ratios and concentrations on axillary bud induction, proliferation and re-rooting were studied using annual semi-lignified branch ...To optimize the regeneration system of Bougainvillea, the effects of different hormone ratios and concentrations on axillary bud induction, proliferation and re-rooting were studied using annual semi-lignified branch cuttings of Bougainvillea ‘Yunnan Purple’ as experimental materials. The results showed that MS+6-BA 2.5 mg/L+NAA 0.1 mg/L was the optimal medium for stem axillary bud initiation, and the initiation rate reached 91.3%. The optimal medium for axillary bud proliferation was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L, and the proliferation coefficient was 3.28. The optimal rooting medium was 1/2 MS+IBA 1.0 mg/L, with the rooting rate of 90% and the average root number of 7.4. After 15 d of hardening seedling, the survival rate of the sterile seedlings was 97.83%. This study laid a basis for rapid propagation and genetic transformation system of Bougainvillea.展开更多
This study aimed to evaluate the potential use of Thai rice for the production of prebiotic isomalto-oligosaccharides through fungal fermentation. Solid-state fermentations of two rice varieties, waxy rice RD6 and non...This study aimed to evaluate the potential use of Thai rice for the production of prebiotic isomalto-oligosaccharides through fungal fermentation. Solid-state fermentations of two rice varieties, waxy rice RD6 and non-waxy rice KDM 105 with Aspergillus oryzae TISTR 3108 were compared. The effects of the main parameters such as incubation time, temperature, pH, fungal spore inoculum size and moisture content were also studied individually to maximise the isomalto-oligosaccharides yield. Results showed that the maximum values of amylolytic activity and total reducing sugar were observed when using rice in SSF with initial moisture content of 70% and inoculated with the inoculum size of 107 spores/g. The optimal conditions of SSF were performed at initial pH 6 and 30 ~C for 5 d. SSF of waxy rice RD6 with Aspergillus oryzae produced highest concentrations of isomalto-oligosaccharides which consisted of isomaltose, panose and isomaltotriose. After fermentation, mashing was used to further hydrolyse the remaining starch in rice slurry. The subsequent rice syrup contained high amounts of isomaltose, panose and isomaltotriose with the values of 44, 10 and 7 g/L respectively.展开更多
基金Supported by the Youth Fund of Heilongjiang University,China~~
文摘[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and external morphology. [Method] The paraffin section method was used to observe the flower bud of Gentiana triflora un- der microscope. [Result] The flower bud differentiation process of Gentiana triflora could be divided into six stages： the undifferentiated stage, initial differentiation stage, sepal primordia differentiation stage, petal primordia differentiation stage, stamen pri- mordia differentiation stage and the pistil primordia differentiation stage. The flower bud differentiation started from early May, when the plant had 10-11 stem nodes, and completed in early June, lasted about 40 d. The effect of plant height on flower bud differentiation was not significant. [Conclusion] The study provided theoretical basis for the high-yield cultivation, variety selection and flowering regulation of Gentiana triflora.
文摘[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of triazophos manufacture. Then the strain C-Y106 was identified according to the morphology,physiological and biochemical characteristics,and 16S rRNA sequence analysis. The effect of medium with different nutrients on triazophos-degrading rate by C-Y106 was studied. [Result] The strain C-Y106 was identified as Bacillus subtilis. The strain C-Y106 could grow in the mineral salt medium with 40 mg/L of triazophos as the sole sources of carbon,Nitrogen and Phosphorus. The triazophos-degrading rate was the highest as 76.8% in the mineral salt medium with 40 mg/L of triazophos as the sole source of Phosphorus,after being incubated at 31 ℃,pH 8.0 and 150 r/min for 60 h. [Conclusion] The research had provided theoretical basis for the identification and purification of enzymes for triazophos degradation.
文摘To optimize the regeneration system of Bougainvillea, the effects of different hormone ratios and concentrations on axillary bud induction, proliferation and re-rooting were studied using annual semi-lignified branch cuttings of Bougainvillea ‘Yunnan Purple’ as experimental materials. The results showed that MS+6-BA 2.5 mg/L+NAA 0.1 mg/L was the optimal medium for stem axillary bud initiation, and the initiation rate reached 91.3%. The optimal medium for axillary bud proliferation was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L, and the proliferation coefficient was 3.28. The optimal rooting medium was 1/2 MS+IBA 1.0 mg/L, with the rooting rate of 90% and the average root number of 7.4. After 15 d of hardening seedling, the survival rate of the sterile seedlings was 97.83%. This study laid a basis for rapid propagation and genetic transformation system of Bougainvillea.
文摘This study aimed to evaluate the potential use of Thai rice for the production of prebiotic isomalto-oligosaccharides through fungal fermentation. Solid-state fermentations of two rice varieties, waxy rice RD6 and non-waxy rice KDM 105 with Aspergillus oryzae TISTR 3108 were compared. The effects of the main parameters such as incubation time, temperature, pH, fungal spore inoculum size and moisture content were also studied individually to maximise the isomalto-oligosaccharides yield. Results showed that the maximum values of amylolytic activity and total reducing sugar were observed when using rice in SSF with initial moisture content of 70% and inoculated with the inoculum size of 107 spores/g. The optimal conditions of SSF were performed at initial pH 6 and 30 ~C for 5 d. SSF of waxy rice RD6 with Aspergillus oryzae produced highest concentrations of isomalto-oligosaccharides which consisted of isomaltose, panose and isomaltotriose. After fermentation, mashing was used to further hydrolyse the remaining starch in rice slurry. The subsequent rice syrup contained high amounts of isomaltose, panose and isomaltotriose with the values of 44, 10 and 7 g/L respectively.
