针灸足三里穴的临床疗效及其胃与结肠电的关系

本研究采用临床疗效观察与动物实验相结合的方法。动物实验采用胃和结肠壁浆膜上缝植Ag-Agcl乏极化电极双极导联法,同时描记针刺“足三里”前后家兔空腹胃和结肠肌电变化。结果证明针刺足三里对胃肠功能有双向调节作用,对腹泻,便秘,肠痉挛等消化系病有良好疗效,总有效率96.7％。本文还首次揭示了胃电兴奋时结肠电抑制,结肠电兴奋时胃电抑制的相关变化规律。

电针刺“足三里”对脾气虚大鼠下丘脑CaM/CaMKⅡ和NPY表达影响随机平行对照研究

[目的]观测电针刺"足三里"对脾气虚大鼠下丘脑CaM/CaMKⅡ和NPY表达影响。[方法]使用随机平行对照方法,将32只SPF级SD大鼠随机数字表分为4组,空白对照组、脾气虚组、足三里组和非经非穴组,8只/组。参照劳倦过度和饮食不节复合因素复制脾气虚大鼠模型:实验第7d,先饱食1日,再禁食2日,3日为1个周期,每日负重游泳(水温35～37℃,5%体重保险丝绑于大鼠尾部)至力竭(即大鼠鼻尖没入水面10秒),连续5个周期;正常组每日自由饮水进食。实验第3d开始干预,1次/d,持续7d;足三里组:毫针(0.25×13mm)直刺双侧"足三里"5mm,接电针仪(SDZ-Ⅱ型),疏密波刺激(密波15Hz,疏波2Hz),电流0.5mA,连续刺激20min;非经非穴组:毫针(0.25×13mm)直刺双侧"非经非穴点"-双侧髂嵴上10～15mm、后正中线旁开20mm区段内一个固定对照点,平刺8～10mm,电针处理同足三里组;空白对照组和脾气虚组在操作台上固定20min,无处理。每日测量大鼠体质量,实验第30d,处死,取丘脑,荧光定量PCR法检测下丘脑组织中CaM/CaMKⅡ及NPY的mRNA表达。[结果]实验第1d至第8d,体质量空白对照组呈稳定增长趋势,脾气虚模型组、足三里组、非经非穴组轻度下降;实验第8d,脾气虚模型组、足三里组、非经非穴组均低于空白对照组,足三里组高于脾气虚模型组;非经非穴组低于足三里组,与脾气虚模型组无明显差异。实验第8d,大鼠下丘脑CAM/CAMKⅡ、NPY足三里组均高于空白对照组、脾气虚组、非经非穴组(P<0.01);非经非穴组、脾气虚组低于空白对照组(P<0.01);非经非穴组与脾气虚组无明显差异(P>0.05)。[结论]电针刺脾气虚大鼠"足三里"可提高下丘脑CaM/CaMKⅡ和NPY表达,提高体质量,明显改善神疲乏力、被毛稀疏无光泽、大便稀溏、体质量逐渐下降等"脾气虚"表现,提示针刺"足三里"对脾气虚良性调节作用可能是通过提高下丘脑CAM/CAMKⅡ、NPY表达水平来调控的。

Effect of acupuncture at Bǎihuì (百会GV 20) and Zúsānlǐ (足三里ST 36) on the level of serum inflammatory cytokines in patients with depression

Objective To investigate the immunologic mechanism of acupuncture at Bǎihuì（百 会 GV 20） and Zúsānlǐ（足三里 ST 36） for treatment of depression. Methods Eighty-four cases of depression patients were randomly divided into an electroacupuncture observation group（group A）, an electroacupuncture control group（group B） and a medication control group（group C）, 28 cases in each group. Bǎihuì（百会 GV 20） and Zúsānlǐ（足三里 ST 36） were used in the group A; Tàichōng （太冲 LR 3）, Sānyīnjiāo （三阴交 SP 6）, Nèiguān （内关 PC 6） and Shénmén （神门 HT 7） were used in the group B; and the group C was treated with oral administration of Fluoxetine. The scores of Hamilton Depression Scale （HAMD） were tested and the level of serum interleukin 1（IL-1β）, interleukin 6 （IL-6）, and tumor necrosis factor α （TNF-α） were measured by Enzyme Linked Immunosorbent Assay （ELISA）before and after treatment. Results Their scores of HAMD were obviously decreased after treatment in three groups（all P0.01）. In the group A, 2 cases were cured, 19 cases were markedly effective, 5 cases were effective and 2 cases were failed. In the group B, 16 cases were markedly effective, 12 cases were effective. While in the group C, 1 case was cured, 17 cases were markedly effective, 7 cases were effective and 3 cases were failed. The grade distribution of clinical effect showed that the effect of group A was better （P0.05）. The levels of serum IL-1β and IL-6 in the three groups were obviously decreased after treatment （P0.05, P0.01）, while there was no significant difference between the level of serum TNF-α before and after treatment. Conclusion The effect of electroacupuncture observation group is superior to those of electroacupuncture control group and medication control group.All of these three methods can clear the inflammatory cytokines such as IL-1β and IL-6 away and improve the symptoms of depression.

艾灸防治绝经后骨质疏松的实验研究

目的观察艾灸对可造成卵巢切除大鼠骨质疏松的各项指标的影响,探讨艾灸对绝经后妇女骨质疏松的治疗作用。方法将30只大鼠随机分为对照组、模型组、艾灸组,每组10只。除正常对照组外,其余两组大鼠摘除双侧卵巢。3 d后对艾灸组经行治疗,三组均正常摄食饮水,之后观察并测定每组大鼠血清雌二醇(E2)、骨钙素(BGP)含量及尿Ca、肌酐(Cr)的含量。结果卵巢切除的模型组与正常对照组相比血清E2明显下降(P<0.05);艾灸组治疗后与模型组比较E2水平有显著差异(P<0.05),与正常对照组E2水平没有显著差异(P>0.05);艾灸组的血清骨钙素明显高于模型组与对照组。尿钙与肌酐的比值艾灸组明显低于模型组(P<0.05),而与正常对照组比较差异无统计学意义。结论艾灸对骨质疏松症具有明显的防治作用,值得临床应用推广。

Effect of purulent moxibustion on Zúsānlǐ(足三里ST 36) point on the clinical symptoms of patients with malignant tumor

Objective To discuss the effect of purulent moxibustion on Zusanli （足三里 ST 36） point on the clinical symptoms and life quality of patients with malignant tumor. Methods Sixty patients with malignant tumor were divided into an observation group and a control group with 30 cases in each group. The observation group was treated with purulent moxibustion on Zusanli （足三里 ST 36） in combination with herbal medicine; the control group was only treated with herbal medicine. After 80 days, the changes of the clinical symptoms and KPS of the two groups before and after the treatment were observed. Results The effective rates for the improvement of lassitude, poor appetite and insomnia in the observation group were 72.4% （21/29） ,25.0% （15/20） ,71.4% （15/21）, and those in the control group were 42.9%（12/28） ,55.6% （10/18） ,42.9% （6/14）, and there was no statistical significance of difference between the two groups（P〉0.05）. KPS has statistical significance in the two groups after the treatment （P〈0.01）,and the observation group was higher than the control group. Conclusion Purulent moxibustion on the point of Zusanli （足三里 ST 36） has certain effect to improve the clinical symptoms of patients with malignant tumor, and raise KPS and upgrade the life quality of patients.

Protective effect of electroacupuncture preconditioning at zúsānlǐ(足三里 ST36) on mitochondria in the intestinal ischemia/reperfusion injury

Objective： The study explored the effect of applying electroacupuncture（EA） preconditioning at ST 36 on mitochondria in rats with intestinal ischemia/reperfusion injury.Methods： Forty SD rats were divided into four sets： sham operation group（sham group）; intestinal ischemia/reperfusion group（I/R group）; EA preconditioning at ST 36 followed by intestinal ischemia/reperfusion injury（ST 36 ＋ I/R group）; EA preconditioning at the lateral site away from ST360.5 cm followed by intestinal ischemia/reperfusion injury（N＋I/R group）. For the sham group, the rats were opened abdominal cavity for 3 h and 20 min and their abdominal cavities were covered with wet gauze avoiding drying and kept on the thermostat at 37 0 C. For the ischemia/reperfusion（I/R） group,rats were anaesthetised and their abdominal cavities were opened to expose jejunum segments. The segment＇s collateral blood supply was restricted by bilateral ligation of the intestine. Next, one of the branches of a mesenteric artery was occluded with a thread for 20 min and then the thread was released after such ischemia conditions, keeping reperfusion for 3 h. For the ST36 ＋ I/R group, the electroacupuncture at ST36 was first performed, then the intestinal ischemia/reperfusion model was constructed. For the N ＋ I/R group, electroacupuncture at non ST36 acupoint, which is away from ST36 about 0.5 cm, and then the intestinal ischemia/reperfusion model was performed. Measurements of the levels of inflammatory markers tumour necrosis factor a（TNFa） and interleukin-1 beta（IL-1β）, cytochrome c（CYCS）, and the mitochondrial membrane pro-apoptotic protein（BAX）, anti-apoptotic protein Bcl-2 were performed.Results： Compared to I/R group, the intensity of cytoplasmic CYCS in intestinal tissues was significantly decreased in the ST 36 ＋ I/R group（1.65 vs. 0.18, p〈0.05）. Compared to N ＋ I/R group, the intensity of cytoplasmic CYCS in intestinal tissues was also dramatically declined in the ST 36 ＋ I/R group（1.37 vs. 0.18, p〈0.05）. The level of CYCS in mitochondria in rats in the ST 36 ＋ I/R group were appreciably increased than those of rats in the I/Rgroup（1.42 vs. 0.06, p〈0.05）, and CYCS in mitochondria was also largely expressed in ST36 ＋ I/R group than N ＋ I/R group（1.42 vs. 0.08, p〈0.05）. Bcl-2 was shown to be elevated in the ST 36 ＋ I/R group than I/R group（1.01 vs. 0.10） and N ＋ I/R group（1.01 vs. 0.09, all p〈0.05）, whereas BAX expression was greatly decreased in the ST36 ＋ I/R group than I/R group（0.11 vs.0.78） and N ＋ I/R group（0.11 vs. 0.87, all p〈0.05）.Conclusion： The results suggest the EA intervention has a protective effect upon mitochondria, preventing CYCS release and the subsequent activation of downstream apoptosis pathway. It is proposed that patients due to undergo gastrointestinal surgery get benefit from EA preconditioning at ST 36.

针灸治疗术后恶心呕吐概况

术后恶心呕吐（PONV）对患者生理及心理都有影响,既延长住院天数,又增加治疗费用,尤其是肥胖、吸烟、腹腔镜手术等危险因素,发生率明显高于一般患者;药物治疗效果不佳,且有副作用,依从性差,非药物治疗成为新选择。针灸可刺激机体免疫功能,反馈至大脑呕吐中枢;促使内啡肽释放止吐;也可通过神经-内分泌系统调节,影响5-HT3转运;针刺时大脑中枢特定区域会发生相应神经系统改变;增强迷走神经调节作用。术前、术中持续刺激穴位获得满意疗效均有报告,尚未形成统一认识,有近部选穴,远部选穴,辨证选穴,配穴按经脉（本经、表里经、同名经）、按部位（上下、前后、左右）,考虑手术因素,多选择四肢部位相关联穴位,常用内关、足三里、三阴交。＂刺之要,气至而有效＂＂得气＂可激活大脑皮质A10神经分布领域,激活旧皮质同时也激发新皮质活性,对脑细胞有广泛影响。留针时间长短与针刺部位、病情、体质、疾病相关,一般留针10~20min。

Effects of Warm Needling at Zusanli(ST 36)on NO and IL-2 Levels in the Middle-Aged and Old People

42 middle-aged and old people at the age between 55-70 years were selected and given the warm needling at Zusanli (ST 36), and their IL-2 and NO contents of peripheral blood before and after acupuncture were determined. The results showed that IL-2 and NO contents increased significantly after the warm needling (P<0.01).

Clinical Study on the Needling and Drug Treatment of Acute Cerebral Hemorrhage

Researches in recent years have shown that cellular immune factor plays an important role in the generation and development of cerebral hemorrhage1-3.

EXPERIMENTAL STUDY ON MOXIBUSTION AT ZUSANLI(ST 36) AND LIANGMEN (ST 21) INDUCING HEAT SHOCK PROTEIN 70 (HSP70) EXPRESSION TO RESIST OXIDATIVE INJURY OF GASTRIC MUCOSA

To observe effect of moxibustion at Zusanli （足三里 ST 36） and Liangmen （梁门 ST 21 ） on expression of heat shock protein 70 （HSP70） in gastric mucosa of the rat of stress ulcer （SU） to explore the mechanism of moxibustion in resisting oxidative injury of the gastric mucosa. Methods： Sixty SD rats were evenly randomized into 4 groups, a blank group, a model group, an acupoint moxibustion group and a non-acupoint moxibustion group. Water restraint stress （WRS） method was used to make stress gastric ulcer rat model. The ulcerative index （Ul） of gastric mucosa was evaluated by using GUTH method, the gastric mu- cosa blood flux （GMBF） was detected by a laser Doppler bloodflow monitor, and HSP70 expression and malondialdehyde （MDA） content in the gastric mucosa were determined respectively with immunohistochemical and thiobarbiturate methods. Results： Moxibustion at Zusanli （ST 36） and Liangmen （ST 21 ） significantly de- creased Ul, up-regulated HSP70 expression, increased GMBF, and decreased MDA content in the gastric mucosa in the rat of stress gastric ulcer, with significant differences as compared with the model group and the non-acupoint moxibustion group （P 〈 0.05 or P 〈 0.01 ）. Conclusion： Moxibustion at Zusanli （ST 36） and Liangmen （ST 21 ） can induce high expression of HSP70 and decrease MDA content in the gastric mucosa, so as to resist oxidative injury, with relative acupoint specificity.

CLINICAL APPLICATION OF NEIGUAN (内关 PC 6) IN THE TREATMENT OF DISORDERS OF THE DIGESTIVE SYSTEM——THE RELATED RESEARCH PROGRESS

In the present paper, the authors review recent situations of clinical application of Neiguan (PC 6内关), etc. in the treatment of disorders of the digestive system and its related research progresses. In clinical practice, Neiguan (PC 6) is usually selected as one of the main acupoints to treat gastrointestinal reactions induced by ① nausea and vomiting, ② hiccup, ③ gastrointestinal dyskinesia, ④ gastritis, and ⑤ intra-gastric examination, cesarean section, etc. Concerning experimental studies, the effects of Neiguan (PC 6), etc. on ① gastric electrical activities, ② gastrointestinal motion, ③ gastrointestinal hormone secretion, and ④ gastric mucosal structure were observed. Results indicated that Neiguan (PC 6) is an important and effective acupoint for treatment of gastrointestinal disorders in clinic. Stimulation of Neiguan (PC 6) induced favorable regulation of both the peripheral nervous system and central nervous system, and changes of the gastrointestinal hormone secretion may contribute to its effects in treating various disorders.

OBSERVATION ON THE EFFECT OF MOXIBUSTION ON HYPERBILIRUBINEMIA IN HEPATITIS B TYPE CIRRHOSIS PATIENTS

Objective: To study the effect of moxibustion on hyperbilirubinemia in hepatitis B cirrhosis patients. Methods: 56 cases of inpatients with hepatitis B cirrhosis were divided into treatment group (n=27) and control group (n=29) randomly. All the patients of these two groups were given with routine expectant treatment including administration of medicines (Bifendate, Eessentiale, Potenline, etc) for protecting liver functions, reducing the level of alanine aminotransferase (ALT), etc., and in the meantime, patients of the treatment group were also treated with moxibustion of Zusanli (足三里 ST 36), Taichong (太冲 LR 3) and Sanyinjiao (三阴交 SP 6), once daily, continuously for 4 weeks. Results: After 4 weeks of treatment, of the 27 and 29 cases of treatment and control group, 23 and 10 patients had improvement in clinical symptoms, 4 and 19 failed, with the total effective rates being 85.18% and 34.48% respectively. Serum total bilirubin (TBil) contents of treatment and control groups decreased significantly, and the level of TBil in the treatment group was significantly lower than that in the control group (P<0.01). Conclusion: Moxibustion is an effective remedy in relieving hyperbilirubinemia and improving clinical symptoms in the treatment of hepatitis B cirrhosis patients.

CLINICAL OBSERVATION ON THE TREATMENT OF KNEE OSTEOARTHRITIS BY ACUPUNCTURE IN BA-HE TECHNIQUE

Objective: To observe the clinical therapeutic effect of Ba-He techniqu e of acupuncture in the treatment of knee osteoarthritis. Methods: A total of 90 cases of knee osteoarthritis were evenly randomized into Ba-He technique group (observation group) and common technique group (control group), with 45 cases in each group. Zusanli (足三里 ST 36), Heding (鹤顶 EX-LE 2) and Dubi (犊鼻 ST 35 ) were punctured respectively in Ba-He technique and common technique in the two gr oups. The therapeutic effects of the two groups were compared after one course o f treatment (10 sessions) in accordance with Japanese assessment criteria for kn ee-joint functions. Results: After treatment, among the 74 and 7 1 affected knees in the observation and control groups, the therapeutic effect was excellent in 42 (56.8%) and 26 (36.6%) knees, fine in 14 (18.9%) and 20 (28.2%), OK in 10 (13 .5%) and 11 (15.5%), and poor in 8 (10.8%) and 14 (19.7%) respectively. The ther apeutic effect of the observation group was significantly better than that of co ntrol group (P<0.01). Conclusion: The Ba- He technique of acupuncture applied to the above-mentioned three acupoints exerted remarkable therapeutic effect for k n ee osteoarthritis, which is obviously better than that of the common needling te chnique.

CLINICAL OBSERVATION ON THE THERAPEUTIC EFFECT OF ACUPUNCTURE OF JIAJI (EX-B2) IN THE TREATMENT OF APOPLECTIC SPASTIC HEMIPLEGIA

Objective: To compare the therape utic effects of acupuncture of different acupoints in the treatment of spast ic hemiplegia of apoplexy. Methods: Eighty stro ke patients with spastic hemiplegia were evenly randomized into control grou p and treatment group. Forty cases of treatment group were given with acupun cture of Jiaji (夹脊 EX-B2) from T1 to L5, while forty cases of control gro up were treated with conventional acupoints as Jianyu (肩NFDA1 LI 15), Quchi (曲池 LI 11), Huantiao (环跳 GB 30), Zusanli (足三里 ST 36), Kunlun (昆仑 BL 60), etc. o n the diseased side, once daily, with 10 sessions being one therapeutic course, and 3 courses all together. The modified Ashworth Scale (MAS) and neurological d eficit grading scale (NDS) were used as the indexes to assess the therapeutic ef fects. Results: After treatment, the scores of NDS of both treat ment and control groups reduced and the difference value between post- and pre-treatment of tr ea tment group was significantly bigger than that of control group. MAS showed a si milar tendency in both groups. Of the 40 cases in treatment and control groups, 6 and 1 were cured, 20 and 15 had marked improvement, 9 and 11 had improvement, 4 and 8 failed, 1 and 5 got worse, with the total effective rates being 87.5% an d 67.5% respectively. The therapeutic effect of treatment group was significantl y superior to that of control group (P<0.01). Conc lusion: Acupuncture of Jiaji ( EX-B2) is superior to acupuncture of conventional acupoints in alleviating the state of spastic hemiplegia of apoplexy.

EFFECT OF ACUPUNCTURE OF "ZUSANLI" AND "WEISHU" ACUPOINTS ON EXPERIMENTAL GASTRIC ULCER

Objective: To observe the therapeutic effect of electroacupuncture (EA) of "Zusanli"(ST 36) and "Weishu"(BL 21)on the experimental gastric ulcer in Xu period of a day for analyzing the best opportunity of acupuncture treatment of gastric ulcer. Methods:49 Wistar rats were randomly divided into ① control group, ② ST 36 Xu group, ③ BL 21 Xu group, ④ ST 36 Chen group, ⑤ BL 21 Chen group, ⑥ ST 36 IT group, and ⑦ BL 21 IT group, with 7 rats in each group. Rat gastric ulcer model was established using intra gastric administration of glacial acetic acid. Gastric mucosal thickness,mucosal muscular defect width,superficial mucosal mucus index (MI),intramucosal MI ,Total MI,plasma 6 keto PGF1α ,serum NO and gastric tissue CGRP concentrations were used as the indexes. The EA treatment was conducted once daily at Xu period (7-9 o’clock in the evening), Chen period (8-10 o’clock in the morning) and irregular time (IT) with 6 days being a therapeutic course, two courses altogether. Results:After EA treatment,①the regenerated mucosal thickness values of the aforementioned 6 EA groups, particularly ST 36 Xu group and BL 21 Xu group, were all apparently higher than that of control group (P<0.05-0.01), while the mucosal muscle layer defect width values of different EA groups, particularly ST 36 Xu group and BL 21 Xu group, were strikingly lower than that of control group (P<0.05-0.01); ②the 3 MI of the 6 EA groups, particularly those of ST 36 Xu group were all significantly higher than those of control group (P<0.05-0.01); ③plasma 6 keto PGF1α contents of the 6 EA groups, particularly those of ST 36 Xu and BL 21 Xu groups, were all significantly higher than that of control group (P<0.05-0.01); ④serum NO and gastric tissue CGRP concentrations of the 6 EA groups, particularly those of ST 36 Xu and BL 21 Xu groups and CGRP concentration of ST 36 Chen group, were all significantly higher than those of control group (P<0.05-0.01);and ⑤there were positive correlation between superficial MI or total MI and NO level (r=0.858, 0.987, P<0.05,and 0.01),and between the superficial mucus index and CGRP concentration (r=0.9051, t=4.68, P<0.01), suggesting that the increase of the secretion function of the regenerated mucosal mucus is related to acupuncture induced increase of release of both NO and CGRP.Conclusion:The therapeutic effect of EA of ST 36 and BL 21 in Xu period is better in the treatment of gastric ulcer,and that of EA of ST 36 is the best.

Acupuncture Treatment of Melancholia

Case HistoryMs. Liu, a 63-year-old housewife, paid her first visit on Oct.7, 1986 with main complaint of distension and pain in the chest and hypochondrium, and insomnia for one month.

Effect of electroacupuncture on hepatic blood flow and lipid peroxidation in rats with sepsis

Objective To investigate protective effects of electroacupuncture on injury of lipid peroxidation-induced by liver ischemia in rats with sepsis.Methods Forty-eight male SD rats were subjected to sepsis by cecal ligation and puncture （CLP）,and were randomly divided into a sham operation group （group A）,a CLP model group （group B）,a CLP model plus electroacupuncture （EA） at Zúsānlǐ （足三里 ST 36） group （group C）,a CLP model plus electroacupuncture at a sham acupoint group （group D）,a CLP model with vagotomy group （group E） and a CLP model plus electroacupuncture group after vagotomy （group F）,with 8 rats in each group.CLP was performed after abdominal vagotomy in group E and group F.EA （2 mA,2/100 Hz） was applied at bilateral ＂Zúsānlǐ＂ （足三里 ST 36） in group C and group F,and at sham acupoints in group D,respectively,for 1 hour.The hepatic blood flow （HBF） was detected by a laser-doppler flowmetry at 6 hours after CLP.The activity of plasma alanine transarninase （ALT） was determined;contents of hepatic malondialdehyde （MDA）,activity of xanthine oxidase （XOD） and the rate of water content in the hepati tissue were assessed,respectively.Results Hepatic blood flow was （56.97±11.95） U in group C,which was significantly lower than （80.12± 19.57） U shown in group A,but higher than （42.61±10.97） U in group B,（44.53±9.23） U in group D,（30.05 ±4.46） U in group E and （30.46±6.38） U in group F （all P0.05） at 6 hours after CLP.Meanwhile,the levels of MDA,XOD,ALT and hepatic water content in group C were all significantly higher than those in group A,but lower than those in the other four groups （all P0.05）.The levels of MDA,XOD,ALT and hepatic water content in group E and group F were all significantly higher than those in group D （all P0.05）,while their hepatic blood flow were lower than that in group D （both P0.05）,and no significant differences were found in all the indices mentioned above between group E and group F （all P0.05）.Conclusion Electroacupuncture at ＂Zúsānlǐ＂ （足三里 ST 36） can improve hepatic ischemia,inhibit lipid peroxidation and alleviate hepatic edema and dysfunction in septic rats,which might be related with the completeness of vagus nerves.

Therapeutic observation on electroacupuncture at Zusanli(ST 36) and Fenglong(ST 40) for senile dementia

Objective： To explore the clinical effects of electroacupuncture（EA） at Zusanli（ST 36） and Fenglong（ST 40） in treating senile dementia. Methods： A total of 74 patients were randomly divided into an EA group and a medication group based upon the random digital table, 37 cases in each group. EA at Zusanli（ST 36） and Fenglong（ST 40） was given in the EA group, once every day, for six treatments per week. Donepezil Hydrochloride Tablets were given to the medication group, 5 mg per time and once a day in the former four weeks, 10 mg per time and once a day after 4 weeks, oral administration before sleep at night. The courses of the treatment were 12 weeks in both groups. The scores of mini-mental state examination（MMSE） and Barthel index（BI） were observed before and after the treatment, for processing the comparative analysis of the clinical effects after the course of the treatment. Results： The total effective rate was 86.5% in the EA group and 70.3% in the medication group. The therapeutic effect was better in the EA group than that in the medication group, and the difference between the two groups was statistically significant（P〈0.05）. MMSE and BI scores after the treatment in the two groups were all elevated than those of the same groups before the treatment（P〈0.05, P〈0.01）. The improving situation was obviously better in the EA group than that in the medication group, and the difference between the two groups was statistically significant（P〈0.05）. Conclusion： EA at Zusanli（ST 36） and Fenglong（ST 40） is affirmative in the therapeutic effect for senile dementia and can also improve the cognitive function and enhance the patients＇ quality of life.

Effect of ear acupuncture plus injection at Zusanli(ST 36) on shoulder pain and cytokines after gynecologic laparoscopic surgery

Objective： To explore the clinical efficacy of ear acupuncture plus injection at Zusanli（ST 36） in treating shoulder pain after laparoscopic gynecological surgery, and to observe its effect on cytokines.Methods： Two hundred patients with shoulder pain after laparoscopic gynecological surgery were randomized into two groups based on their visiting sequence, 100 cases each. The observation group was intervened by ear acupuncture plus injection at Zusanli（ST 36）, and the control group was intervened by oral administration of Ibuprofen, 10 d as a treatment course. The clinical efficacies of the two groups were compared after 2 treatment courses; the visual analogue scale（VAS）,present pain intensity（PPI） and 36-item short-form health survey（SF-36） were measured before and after the treatment;the changes of interleukin（IL）-6 and IL-10 after the treatment were also observed.Results： The VAS and PPI scores were significantly changed after the treatment in both groups（both P〈0.01）. After the treatment, the VAS score in the observation group was significantly different from that in the control group（P〈0.05）. The component scores of SF-36 were significantly changed after the treatment in both groups（P〈0.01）; after the treatment, the scores of physical functioning（PF）, bodily pain（BP）, social functioning（SF）, and mental health（MH） in the observation group were significantly different from those in the control group（all P〈0.05）. The contents of IL-6 and IL-10 dropped significantly after the intervention in both groups（both P〈0.01）, and the between-group differences were also statistically significant（both P〈0.01）. The total effective rate of the observation group was higher than that of the control group（P〈0.05）.Conclusion： Ear acupuncture plus injection at Zusanli（ST 36） can significantly improve the shoulder pain after laparoscopic gynecological surgery, down-regulate the expressions of IL-6 and IL-10, and boost the recovery.

Instant effect of moxibustion at different points on electrogastrogram of patients with functional dyspepsia

Objective： To observe different effects of moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） on electrogastrogram （EGG） of patients with functional dyspepsia （FD）. Methods： A total of 65 cases were randomized into a Zusanli （ST 36） group （n=33） and a Yanglingquan （GB 34） group （n=32） by random serial number, to receive moxibustion therapy to the corresponding point respectively. The 8-lead EGG was used before and after treatment to collect EGG parameter of the gastric body, antrum of stomach, lesser curvature and greater curvature, including the dominant frequency, slow wave percentage, average frequency, response area and motility index. Results： After treatment, the dominant frequency and slow wave percentage were improved in nearly the whole stomach area in Zusanli （ST 36） group, showing statistical significances （both P〈0.05）; the slow wave percentage and average frequency were improved in stomach body and antrum of stomach in Yanglingquan （GB 34） group, showing statistical significances （both P〈0.05）; moxibustion at Zusanli （ST 36） had a significantly higher influence on EGG than Yanglingquan （GB 34）, in which the lifting of slow wave percentage was significantly higher than Yanglingquan （GB 34） group （P〈0.05）. Conclusion： Moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） has the effect of adjusting abnormal EGG pattern in FD patients. Compared with Yanglingquan （GB 34）, Zusanli （ST 36） has a superior effect in improving EGG in stomach area.